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2097 results for "Peptide" - showing 1900-1950
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2OS cells using RAB7A antibody (A12784) at dilution of 1:100. Blue: DAPI for nuclear staining.)
RAB7A, Polyclonal Antibody (Cat# AAA28256)
Full Name
RAB7A Polyclonal Antibody
Gene Names
RAB7A; RAB7; PRO2706
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Aquaporin 4 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Aquaporin 4, Monoclonal Antibody (Cat# AAA27803)
Full Name
Anti-Aquaporin 4 Antibody
Gene Names
AQP4; MIWC; WCH4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Pricing
FCM (Flow Cytometry)
(ENAM Antibody (C-term) (AAA28699) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
ENAM, Polyclonal Antibody (Cat# AAA28699)
Full Name
ENAM Antibody (C-term)
Gene Names
ENAM; ADAI; AI1C; AIH2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(AAA28674 staining GLUL in Human liver tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
GLUL, Polyclonal Antibody (Cat# AAA28674)
Full Name
GLUL Antibody (N-term)
Gene Names
GLUL; GS; GLNS; PIG43; PIG59
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Chicken, Monkey, Pig)
Applications
Western Blot, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(AAA31139 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
POLD4, Polyclonal Antibody (Cat# AAA31139)
Full Name
POLD4 Antibody
Gene Names
POLD4; p12; POLDS
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31231 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H1.5, Polyclonal Antibody (Cat# AAA31231)
Full Name
Histone H1.5 Antibody
Gene Names
H1-5; H1; H1B; H1.5; H1F5; H1s-3; HIST1H1B
Reactivity
Human, Mouse, Rat
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.)
Argininosuccinate synthetase 1, Polyclonal Antibody (Cat# AAA13660)
Full Name
Goat anti-Argininosuccinate synthetase 1 Antibody
Gene Names
ASS1; ASS; CTLN1
Reactivity
Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Applications
Peptide ELISA, Western Blot, Immunofluorescence, Immunoprecipitation, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistochemistry)
(AAA31140 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
HOXC13, Polyclonal Antibody (Cat# AAA31140)
Full Name
HOXC13 Antibody
Gene Names
HOXC13; HOX3; ECTD9; HOX3G
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Peptide affinity purification
Pricing
GFAP, Antibody (Cat# AAA13651)
Full Name
Goat anti-GFAP Antibody
Gene Names
GFAP; ALXDRD
Reactivity
Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog
Expected from sequence similarity: Human, Mouse, Rat, Dog
Applications
Peptide ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
IHC (Immunohistochemistry)
(Western blot analysis of lysates from 293T cell line human brain and mouse brain tissue lysate(from left to right), using STRADA Antibody (C-term). AAA28673 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
STRADA, Polyclonal Antibody (Cat# AAA28673)
Full Name
STRADA Antibody (C-term)
Gene Names
STRADA; LYK5; PMSE; Stlk; STRAD; NY-BR-96
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Applications
Western Blot, Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(AAA30948 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Chk1, Polyclonal Antibody (Cat# AAA30948)
Full Name
Phospho-Chk1 (Ser317) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of lysate from mouse heart tissue lysate, using MYBPC3 Antibody (N-term). AAA28794 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
MYBPC3, Polyclonal Antibody (Cat# AAA28794)
Full Name
MYBPC3 Antibody (N-term)
Gene Names
MYBPC3; FHC; CMH4; CMD1MM; LVNC10; MYBP-C
Reactivity
Human, Mouse, Rat
(Predicted Reactivity: Rat)
(Predicted Reactivity: Rat)
Applications
Immunohistochemistry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(AAA31265 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31265) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
PPP2R5C, Polyclonal Antibody (Cat# AAA31265)
Full Name
PPP2R5C Antibody
Gene Names
PPP2R5C; B56G; PR61G; B56gamma
Reactivity
Human, Rat
Predicted Reactivity: Rabbit(88%)
Predicted Reactivity: Rabbit(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(AAA31059 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Smad2/3, Polyclonal Antibody (Cat# AAA31059)
Full Name
Phospho-Smad2/3 (Thr8) Antibody
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide affinity columns
Pricing
IF (Immunofluorescence)
(staining A549 cells cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Cox2, Polyclonal Antibody (Cat# AAA31143)
Full Name
Cox2 Antibody
Gene Names
PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-HDAC3 (Ser424) Antibody expression in mouse lung and mouse brain tissues lysates.The lane on the right is treated with the antigen-specific peptide.)
HDAC3, Polyclonal Antibody (Cat# AAA30951)
Full Name
Phospho-HDAC3 (Ser424) Antibody
Gene Names
HDAC3; HD3; RPD3; RPD3-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(RSPO2 Antibody (C-term) (AAA28708) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
RSPO2, Polyclonal Antibody (Cat# AAA28708)
Full Name
RSPO2 Antibody (C-term)
Gene Names
RSPO2; CRISTIN2
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Westen Blot, Immunohistochemistry, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(AAA31053 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
GATA4, Polyclonal Antibody (Cat# AAA31053)
Full Name
Phospho-GATA4 (Ser262) Antibody
Gene Names
GATA4; TOF; ASD2; VSD1; TACHD
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of XIAP phosphorylation expression in Anisomycin treated HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
XIAP, Polyclonal Antibody (Cat# AAA31060)
Full Name
Phospho-XIAP (Ser87) Antibody
Gene Names
XIAP; API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30995 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
BCL-2, Polyclonal Antibody (Cat# AAA30995)
Full Name
Phospho-BCL-2 (Ser70) Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of LoVo cell lysate, using PIBF1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
PIBF1, Polyclonal Antibody (Cat# AAA31130)
Full Name
PIBF1 Antibody
Gene Names
PIBF1; PIBF; CEP90; JBTS33; C13orf24
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-Tau (Ser214) expression in various lysates)
Tau, Polyclonal Antibody (Cat# AAA30996)
Full Name
Phospho-Tau (Ser214) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Tau, Polyclonal Antibody (Cat# AAA31002)
Full Name
Phospho-Tau (Thr231) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho- peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30934 at 1/100 staining human TB tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
I kappaB alpha, Polyclonal Antibody (Cat# AAA30934)
Full Name
Phospho-I kappaB alpha (Ser32/Ser36) Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U20S cells using anti-Calpastatin antibody (AAA11679).Overlay histogram showing U20S cells stained with AAA11679 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calpastatin Antibody (AAA11679,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Calpastatin, Polyclonal Antibody (Cat# AAA11679)
Full Name
Anti-Calpastatin Antibody
Gene Names
CAST; BS-17; PLACK
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U251 cells using anti-PNP antibody (AAA11680).Overlay histogram showing U251 cells stained with AAA11680 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PNP Antibody (AAA11680,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight ®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PNP, Polyclonal Antibody (Cat# AAA11680)
Full Name
Anti-PNP Antibody
Gene Names
PNP; NP; PUNP; PRO1837
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Figure 7. IF analysis of CD68 using anti- CD68 antibody CD68 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti- CD68 Antibody overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
CD68, Polyclonal Antibody (Cat# AAA11512)
Full Name
Anti-CD68 antibody
Gene Names
Cd68; Lamp4; gp110; Scard1
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
AOC3 / VAP-1, Polyclonal Antibody (Cat# AAA12319)
Full Name
Goat Polyclonal to Human AOC3 / VAP-1
Gene Names
AOC3; HPAO; SSAO; VAP1; VAP-1
Reactivity
Human, Hamster
Predicted Reactivity: Monkey, Mouse, Rat, Rabbit
Predicted Reactivity: Monkey, Mouse, Rat, Rabbit
Applications
Immunohistochemistry, Immunohistochemistry, Peptide ELISA
Purity
Immunoaffinity Purified
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PAR4, Polyclonal Antibody (Cat# AAA31375)
Full Name
Phospho-PAR4 (Thr163) Antibody
Gene Names
PAWR; PAR4; Par-4
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (92%), Rabbit (100%), Chicken (100%), Xenopus (92%)
Predicted Reactivity: Pig (100%), Zebrafish (92%), Rabbit (100%), Chicken (100%), Xenopus (92%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Chk2 phosphorylation expression in Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Chk2, Polyclonal Antibody (Cat# AAA30957)
Full Name
Phospho-Chk2 (Thr387) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Product Limitations
GATA3, Monoclonal Antibody (Cat# AAA13571)
Full Name
GATA3
Gene Names
GATA3; cGATA-3
Reactivity
Human
Predicted: Rat
Predicted: Rat
Applications
Immunohistochemistry
Pricing
WB (Western Blot)
(Western blot analysis of p53 phosphorylation expression in HU treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
p53, Polyclonal Antibody (Cat# AAA30977)
Full Name
Phospho-p53 (Ser15) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11850)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
Immunohistochemistry, Western Blot
Pricing
ABCB10, Blocking Peptide (Cat# AAA13682)
Full Name
ABCB10 Immunizing Peptide
Gene Names
ABCB10; M-ABC2; MTABC2; EST20237
Reactivity
Human, Mouse, Rat
Pricing
WB (Western Blot)
(Western Blot analysis of KB, Mouse lung using Pan myristoylation Polyclonal Antibody at dilution of 1:1000.)
Pan myristoylation, Polyclonal Antibody (Cat# AAA22115)
Full Name
Pan myristoylation Polyclonal Antibody
Reactivity
All
Applications
Western Blot
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AAA29762(FIZ1 Antibody) at dilution 1/35.(Original magnification: 200))
FIZ1, Polyclonal Antibody (Cat# AAA29762)
Full Name
FIZ1 Antibody
Gene Names
FIZ1; ZNF798
Reactivity
Human, Mouse
Applications
Immunohistochemistry
Purity
Antigen affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of Arylsulfatase B expression in HEK293T (A), NS-1 (B), H9C2 (C) whole cell lysates.)
Arylsulfatase B, Polyclonal Antibody (Cat# AAA27789)
Full Name
Anti-Arylsulfatase B Antibody
Gene Names
ARSB; ASB; G4S; MPS6
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Anti-YFP Ab at 1/2,000 dilution; 293HEK cells transduced with YFP Ad; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)
YFP, Polyclonal Antibody (Cat# AAA13878)
Full Name
YFP Polyclonal Antibody
Reactivity
Transfected cells proteins
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
This antibody is epitope-affinity purified from goat antiserum.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of parafffin-embedded rat spleen using Lysozyme Polyclonal Antibody at dilution of 1:400.)
Lysozyme, Polyclonal Antibody (Cat# AAA22122)
Full Name
Lysozyme Polyclonal Antibody
Gene Names
Lyz2; Lys; Lzm; Lzp; Lysm; Lyzs; Lyzf2; Lzm-s1; AI326280
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts from LOVO cells and HepG2 cells, using CA6 antibody.)
CA6, Antibody (Cat# AAA17978)
Full Name
CA6 Antibody
Gene Names
CA6; CA-VI; GUSTIN
Reactivity
Human (Identities = 100%, Positives = 100%)
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
WB (Western Blot)
(Validation Data Western blot analysis of extracts of various cell lines, using GAA antibody (AAA28197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 60s)
GAA, Polyclonal Antibody (Cat# AAA28197)
Full Name
GAA Polyclonal Antibody
Gene Names
GAA; LYAG
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation was performed with cross-linked chromatin from 293T, using Acetyl-Histone H3-K27 Rabbit pAb antibody (AAA28163) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram constructed the ratios of the ratio of the immunoprecipitated DNA versus the input.)
H3K27AC, Antibody (Cat# AAA28163)
Full Name
Histone H3K27AC Polyclonal Antibody
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
p16, Polyclonal Antibody (Cat# AAA13850)
Full Name
p16 (C20) Antibody
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Pricing
IEM (Immunogold Electron Microscopy)
(Immunogold labeling of RPE, in vivo injected with mCherry expressing vector)
mCherry, Polyclonal Antibody (Cat# AAA13867)
Full Name
mCherry Polyclonal Antibody
Reactivity
Transfected cells proteins
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, Immunohistochemistry, Immunoelectron Microscopy
Pricing
WB (Western Blot)
(Western blot analysis of extracts of rat thymus, using T2R38 antibody (AAA27928) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.)
T2R38, Polyclonal Antibody (Cat# AAA27928)
Full Name
Taste receptor type 2 member 38 (T2R38) (PTC bitter taste receptor) (Taste receptor type 2 member 61)
Gene Names
TAS2R38; PTC; T2R38; T2R61
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
WB (Western Blot)
(Western blot analysis Rpt6 (Phospho-Ser120) using 293 whole cell lysates)
Rpt6, Polyclonal Antibody (Cat# AAA29757)
Full Name
Rpt6 (Phospho-Ser120) Antibody
Gene Names
PSMC5; S8; p45; SUG1; SUG-1; TBP10; TRIP1; p45/SUG
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Pricing
IHC (Immunohistochemistry)
(IHC of human adipose tissue anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:750/DAB detection.)
CANX, Polyclonal Antibody (Cat# AAA13872)
Full Name
Calnexin Polyclonal Antibody
Gene Names
canx; zgc:63524; wu:fe06b12
Reactivity
Human, Rat, Mouse, Monkey, Canine proteins
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, Immunohistochemistry
Pricing
WB (Western Blot)
(25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.)
RIPK3, Polyclonal Antibody (Cat# AAA23385)
Full Name
RIPK3 antibody - N-terminal region
Gene Names
RIPK3; RIP3
Reactivity
Tested Species Reactivty: Human
Predicted Species Reactivity: Cow, Dog, Horse, Human, Mouse, Pig, Rat
Predicted Species Reactivity: Cow, Dog, Horse, Human, Mouse, Pig, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Protein A purified
Pricing
IHC (Immunohistochemistry)
(AAA30941 at 1/100 staining Human lung cancer by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
AC-FKHR, Polyclonal Antibody (Cat# AAA30941)
Full Name
AC-FKHR(K294) Antibody
Gene Names
FOXO1; FKH1; FKHR; FOXO1A
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry
Purity
Peptide affinity purification
Pricing
IHC (Immunohistochemistry)
(Anti-5HT1A Receptor antibody, AAA11520, IHC(P)IHC(P): Rat Brain Tissue)
5HT1A Receptor, Polyclonal Antibody (Cat# AAA11520)
Full Name
Anti-5HT1A Receptor antibody
Gene Names
HTR1A; G-21; 5HT1a; PFMCD; 5-HT1A; 5-HT-1A; ADRBRL1; ADRB2RL1
Reactivity
Reacts with: Human, Rat
Predicted to work with: Mouse
Predicted to work with: Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing