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2304 results for "Secondary Antibody" - showing 1650-1700
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with ADFP antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ADFP, Monoclonal Antibody (Cat# AAA30342)
Full Name
ADFP Antibody
Gene Names
PLIN2; ADFP; ADRP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
ProA affinity purified
Pricing
SDS-PAGE
(The antibody wsas purified by protein G affinity achromatography from cell culture supernatants and verified by SDS-Page (Fig.2).Fig.2: SDS-PAGE analysis of purified YD-4E9 monoclonal antibody.Lane 1: molecular weight markerLane 2: 2ug of purified YD-4E9 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
CLAUDIN9, Monoclonal Antibody (Cat# AAA14098)
Full Name
anti-human Claudin 9 monoclonal antibody
Reactivity
Human
Applications
Flow Cytometry, Competitive ELISA
Purity
Protein G
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human small intestine using AAA26955 at dilution 1:100)
PPM1D, Polyclonal Antibody (Cat# AAA26955)
Full Name
PPM1D Antibody
Gene Names
PPM1D; WIP1; PP2C-DELTA
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Pricing
FCM (Flow Cytometry)
(BDKRB1 Antibody (Center) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
BDK_1, Polyclonal Antibody (Cat# AAA28760)
Full Name
BDK_1 Antibody (Center)
Gene Names
BDKRB1; B1R; BKR1; B1BKR; BKB1R; BDKRB2; BRADYB1
Reactivity
Human
Applications
Western Blot, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western blot analysis of lung1 lysate using FGF10 antibody. The lane on the left is treated with the antigen-specific peptide.)
FGF10, Polyclonal Antibody (Cat# AAA31123)
Full Name
FGF10 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohistchemistry-Paraffin)
(AAA31208 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
SV2A, Polyclonal Antibody (Cat# AAA31208)
Full Name
SV2A Antibody
Gene Names
SV2A; SV2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Applications
ELISA
Purity
Peptide affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of RPL22/L1 using K562 whole cell lysates)
RPL22/L1, Polyclonal Antibody (Cat# AAA31142)
Full Name
RPL22/L1 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
Application Data
(Peripheral dog granulocytes stained with Mouse anti Dog CD107b following permeabilization with Leucoperm (BUF09). Goat anti Mouse IgG (H/L):FITC used as secondary antibody.)
CD107b, Monoclonal Antibody (Cat# AAA12260)
Full Name
Mouse Anti Dog CD107b
Applications
Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
WB (Western Blot)
(SLCO1B3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SLCO1B3 antibody detected the SLCO1B3 protein (arrow).)
SLCO1B3, Polyclonal Antibody (Cat# AAA28785)
Full Name
SLCO1B3 Antibody (C-term)
Gene Names
SLCO1B3; LST3; HBLRR; LST-2; OATP8; OATP-8; OATP1B3; SLC21A8; LST-3TM13
Reactivity
Human
Applications
Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(AAA31206 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31206) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Activin Receptor Type IA, Polyclonal Antibody (Cat# AAA31206)
Full Name
Activin Receptor Type IA Antibody
Gene Names
ACVR1; FOP; ALK2; SKR1; TSRI; ACTRI; ACVR1A; ACVRLK2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31196 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31196) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
PRDM16, Polyclonal Antibody (Cat# AAA31196)
Full Name
PRDM16 Antibody
Gene Names
PRDM16; MEL1; KMT8F; LVNC8; PFM13; CMD1LL
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(Immunofluorescence Validation of cIAP in Human Lung Tissue Immunofluorescent analysis of 4% paraformaldehydefixed human lung tissue abeling cIAP with 3325 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).)
cIAP, Polyclonal Antibody (Cat# AAA10954)
Full Name
cIAP Antibody
Gene Names
BIRC2; API1; MIHB; HIAP2; RNF48; cIAP1; Hiap-2; c-IAP1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
cIAP Antibody is affinity chromatography purified via peptide column.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-PI3K p85 alpha (Tyr607) expression in various lysates)
PI3K p85 alpha, Polyclonal Antibody (Cat# AAA31027)
Full Name
Phospho-PI3K p85 alpha (Tyr607) Antibody
Gene Names
PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA
Reactivity
Human, Mouse, Rat, Pig
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of Adrenergic Receptor beta2 phosphorylation expression in nocodazole treated HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Adrenergic Receptor beta2, Polyclonal Antibody (Cat# AAA30989)
Full Name
Phospho-Adrenergic Receptor beta2 (Ser346) Antibody
Gene Names
ADRB2; BAR; B2AR; ADRBR; ADRB2R; BETA2AR
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
IHC (Immunohistochemistry)
(AAA30985 at 1/50 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Keratin 8, Polyclonal Antibody (Cat# AAA30985)
Full Name
Phospho-Keratin 8 (Ser432) Antibody
Gene Names
KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31083 at 1/100 staining human breast tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 92)
EGFR, Polyclonal Antibody (Cat# AAA31083)
Full Name
EGFR Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
WB (Western Blot)
(Western blot analysis of SARS-COV-2 N Protein using SARS-COV-2 N Protein Rabbit pAb (AAA28308) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
COVID 19 Nucleoprotein Coronavirus, Monoclonal Antibody (Cat# AAA28308)
Full Name
2019-nCoV N Protein Rabbit mAb
Reactivity
Reactivity SARS-CoV-2 Nucleoprotein
Applications
Western Blot, Flow Cytometry, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of lysate from A431 cell line, using SPRR2A Antibody (C-term). AAA28741 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
SPRR2A, Polyclonal Antibody (Cat# AAA28741)
Full Name
SPRR2A Antibody (C-term)
Reactivity
Human
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Host: Rabbit Target Name: FOSL1 Sample Tissue: Human HepG2 Whole Cell Antibody Dilution: 1ug/ml)
FOSL1, Polyclonal Antibody (Cat# AAA23383)
Full Name
FOSL1 antibody - middle region
Gene Names
FOSL1; FRA; FRA1; fra-1
Reactivity
Tested Species Reactivity Human, Rat
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysisi of NIH/3T3 cells using Asymmertric DiMethyl-Histone H3-R2 antibody (AAA10658) at dilution of 1:100.Blue: DAPI for nuclear staining)
H3R2me2a, Antibody (Cat# AAA10658)
Full Name
Histone H3R2me2a Polyclonal Antibody
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity Purification
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27682)
Full Name
VPS35 Antibody, Clone 8A3
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IF (Immunofluorescence)
(AAA31216 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31216) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
FKBP25, Polyclonal Antibody (Cat# AAA31216)
Full Name
FKBP25 Antibody
Gene Names
FKBP3; FKBP-3; FKBP25; PPIase; FKBP-25
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Raji cells stained with (red line) at 1:500. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
CD45, Monoclonal Antibody (Cat# AAA27049)
Full Name
CD45 Monoclonal Antibody
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
>95%, Protein A purified
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human Liver tissue reacted with SYVN1 (HRD1) Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
SYVN1 (HRD1), Polyclonal Antibody (Cat# AAA28764)
Full Name
SYVN1 (HRD1) Antibody (C-term)
Gene Names
SYVN1; DER3; HRD1
Reactivity
Human, mouse
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescence of LOVO cells, using CHRM4 Antibody. The picture on the right is treated with the synthesized peptide.)
CHRM4 / M4, Polyclonal Antibody (Cat# AAA12369)
Full Name
Rabbit Polyclonal (IgG) to Human CHRM4 / M4
Gene Names
CHRM4; HM4; M4R
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunofluorescence, Peptide ELISA
Purity
Immunoaffinity Purified
Pricing
IHC (Immunohistochemistry)
(MCOLN1 Antibody (C-term) (AAA28690)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MCOLN1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
MCOLN1, Polyclonal Antibody (Cat# AAA28690)
Full Name
MCOLN1 Antibody (C-term)
Gene Names
MCOLN1; ML4; MG-2; MLIV; MST080; TRPML1; MSTP080; TRP-ML1; TRPM-L1
Reactivity
Human
Predicted Reactivity: Monkey, Mouse
Predicted Reactivity: Monkey, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(AAA30986 at 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Fyn, Polyclonal Antibody (Cat# AAA30986)
Full Name
Phospho-Fyn (Tyr530) Antibody
Gene Names
FYN; SLK; SYN; p59-FYN
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Antibody (1 ug/ml) staining of HUVEC lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
ROBO1, Polyclonal Antibody (Cat# AAA12322)
Full Name
Goat Polyclonal to Human ROBO1
Gene Names
ROBO1; SAX3; DUTT1
Reactivity
Bat, Bovine, Chicken, Dog, Xenopus, Hamster, Horse, Pig, Human, Monkey, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Pricing
IF (Immunofluorescence)
(AAA31071 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
HER4, Polyclonal Antibody (Cat# AAA31071)
Full Name
Phospho-HER4 (Tyr1284) Antibody
Gene Names
ERBB4; HER4; ALS19; p180erbB4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PRC1, Polyclonal Antibody (Cat# AAA31450)
Full Name
Phospho-PRC1 (Thr481) Antibody
Gene Names
PRC1; ASE1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (80%), Horse (90%)
Predicted Reactivity: Pig (80%), Horse (90%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(AAA31369 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31369 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
FOXM1, Polyclonal Antibody (Cat# AAA31369)
Full Name
Phospho-FOXM1 (Ser35) Antibody
Gene Names
FOXM1; MPP2; TGT3; HFH11; HNF-3; INS-1; MPP-2; PIG29; FKHL16; FOXM1B; HFH-11; TRIDENT; MPHOSPH2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Analysis of Protein Array containing more than 19,000 full-length human proteins using ICOS-L Mouse Monoclonal Antibody (ICOSL/3111). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
ICOS-L/ICOS Ligand/B7RP-1 (Immuno-Oncology Target), Monoclonal Antibody (Cat# AAA23916)
Full Name
ICOS-L/ICOS Ligand/B7RP-1 (Immuno-Oncology Target)
Gene Names
ICOSLG; B7h; B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml
Pricing
FCM (Flow Cytometry)
(HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-FOLH1 antibody, and then analyzed by flow cytometry.)
FOLH1 / PSMA, Monoclonal Antibody (Cat# AAA12376)
Full Name
Anti-FOLH1 / PSMA Antibody (clone 3H5) IHC-plus
Gene Names
FOLH1; PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
Reactivity
Mouse, Human
Applications
Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry
Purity
Protein A/G purified
Pricing
FCM (Flow Cytometry)
(WB - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail FC - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail Overlay histogram showing HepG2 cells stained with AAA28757(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28757, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
MCSF Receptor (CSF1R), Polyclonal Antibody (Cat# AAA28757)
Full Name
MCSF Receptor (CSF1R) Antibody (C-term)
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Mouse Anti-Monkey IgG, Monoclonal Secondary Antibody (Cat# AAA14895)
Full Name
Mouse Anti-Monkey IgG-HRP
Reactivity
Monkey
Applications
ELISA
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using HDAC5 antibody at dilution of 1:100 (40x lens).)
HDAC5, Polyclonal Antibody (Cat# AAA10627)
Full Name
Rabbit polyclonal to Human HDAC5
Gene Names
HDAC5; HD5; NY-CO-9
Reactivity
Human, mouse, rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using cGAS Rabbit pAb (AAA28207) at dilution of 1:50 (40x lens).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
MB21D1, Polyclonal Antibody (Cat# AAA28207)
Full Name
MB21D1 Polyclonal Antibody
Gene Names
MB21D1; cGAS; h-cGAS; C6orf150
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stainedwith Company A (5ug), Company B (5ug), Company C (5ug), Company D (5ug), AAA27031 (5ug) (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 hat 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
TUBA1A, Monoclonal Antibody (Cat# AAA27031)
Full Name
TUBA1A
Gene Names
Tuba1a; Tuba1; Tuba-1
Reactivity
Human, Rabbit, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein A purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of PGD2 Receptor staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
PGD2 Receptor, Polyclonal Antibody (Cat# AAA27807)
Full Name
Anti-PGD2 Receptor Antibody
Gene Names
PTGDR; DP; AS1; DP1; ASRT1; PTGDR1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
LeY, Monoclonal Recombinant Antibody (Cat# AAA14186)
Full Name
Anti-LeY [H18A]
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A affinity purified
Pricing
IF (Immunofluorescence)
(Fig.6. Immunofluorescence analysis of rat kidney tissue. 1, COX IV Monoclonal Antibody (14Y2) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
COX IV, Monoclonal Antibody (Cat# AAA31542)
Full Name
Anti-COX IV Mouse Monoclonal Antibody (14Y2)
Gene Names
COX4I1; COX4; COXIV; COX4-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Pricing
WB (Western Blot)
(All lanes: FOLR4 Antibody (N-term) at 1:500 dilution Lane 1: HepG2 whole cell lysateLane 2: U266B1 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size: 36 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
FOLR4, Polyclonal Antibody (Cat# AAA28763)
Full Name
FOLR4 Antibody (N-term)
Gene Names
IZUMO1R; JUNO; FOLR4; Folbp3
Reactivity
Human
Applications
Western Blot
Pricing
WB (Western Blot)
(All lanes : Anti-TIGIT Antibody at 1:2000 dilutionLane 1: EL4 whole cell lysateLane 2: human fetal thymus lysateLane 3: human spleen lysateLane 4: HUT78 whole cell lysateLane 5: Jurkat whole cell lysateLane 6: MOLT-4 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 26 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
TIGIT, Polyclonal Antibody (Cat# AAA28803)
Full Name
TIGIT Antibody
Gene Names
TIGIT; VSIG9; VSTM3; WUCAM
Reactivity
Human, Mouse
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(Fig.7. Immunohistochemical analysis of paraffin-embedded rat kidney tissue.1, alpha-SMA Monoclonal Antibody was diluted at 1:200 (4 degree C, overnight).2, Sodium citrate pH 6.0 was used for antibody retrieval (>98 degree C, 20min).3, secondary antibody was diluted at 1:200 (room temperature, 30min).Negative control was used by secondary antibody only.)
alpha-SMA, Monoclonal Antibody (Cat# AAA31477)
Full Name
alpha-SMA Monoclonal Antibody
Gene Names
ACTA2; ACTSA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(AAA31188 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31188) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
ARF3, Polyclonal Antibody (Cat# AAA31188)
Full Name
ARF3 Antibody
Reactivity
Human, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Chicken(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31204 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31204 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
MSH4, Polyclonal Antibody (Cat# AAA31204)
Full Name
MSH4 Antibody
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine(100%), Horse(100%), Sheep(100%)
Predicted Reactivity: Bovine(100%), Horse(100%), Sheep(100%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31047 staining RAW264.7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
STAT5A/B, Polyclonal Antibody (Cat# AAA31047)
Full Name
Phospho-STAT5A/B (Ser725/730) Antibody
Gene Names
STAT5A; MGF; STAT5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-Tau (Ser396) expression in various lysates)
Tau, Polyclonal Antibody (Cat# AAA31003)
Full Name
Phospho-Tau (Ser396) Antibody
Gene Names
MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(AAA31372 staining Hela cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31372 1:200) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.)
RUNX2, Polyclonal Antibody (Cat# AAA31372)
Full Name
Phospho-RUNX2 (S340) Antibody
Gene Names
RUNX2; CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with NANOG Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NANOG primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NANOG immunoreactivity is localized to Nucleus significantly.)
NANOG, Polyclonal Antibody (Cat# AAA28706)
Full Name
NANOG Antibody (N-term)
Reactivity
Human (Predicted Reactivity: Monkey)
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing