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Application
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2304 results for "Secondary Antibody" - showing 1600-1650
IF (Immunofluorescence)
(ICC/IF analysis of APP/Protease Nexin II in U87MG cells. The cell was stained with AAA11729 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
APP, Monoclonal Antibody (Cat# AAA11729)
Full Name
APP antibody
Gene Names
APP; AAA; AD1; PN2; ABPP; APPI; CVAP; ABETA; PN-II; preA4; CTFgamma
Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence
Purity
By protein-G affinity chromatography
Pricing
ELISA
(Examples: Interaction against hIgG1 determined by Gator (BLI)*KD=1.2×10-11)
Fab (CH1), Monoclonal Secondary Antibody (Cat# AAA27978)
Full Name
Anti-Human Fab (CH1) mAb, AP
Reactivity
Human
Applications
Western Blot
Pricing
ELISA
(Figure 2 ELISA TestAntibodies: SARS-CoV-2 Nucleocapsid antibody, AAA11029. An ELISA was performed using human SARS-CoV-2 Nucleocapsid recombinant protein as coating antigen and the SARS-CoV-2 Nucleocapsid antibody, as the capture antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 2 ng/mL to 1000 ng/mL.)
COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Antibody (Cat# AAA11029)
Full Name
SARS-CoV-2 (COVID-19) Nucleoprotein
Reactivity
Virus
Applications
Immunofluorescence
Purity
SARS-CoV-2 (COVID-19, 2019-nCoV) Nucleoprotein antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31269 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Snx6, Polyclonal Antibody (Cat# AAA31269)
Full Name
Snx6 Antibody
Gene Names
SNX6; TFAF2; MSTP010
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31251 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Dcp2, Polyclonal Antibody (Cat# AAA31251)
Full Name
Dcp2 Antibody
Gene Names
DCP2; NUDT20
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 11H10. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27707)
Full Name
VPS35 Antibody, Clone 11H10: PerCP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation
Purity
Protein G Purified
Pricing
IHC (Immunohistochemistry)
(Immunofluorescence staining of MCF-7 cells with AAA27006 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
NECTIN4, Polyclonal Antibody (Cat# AAA27006)
Full Name
NECTIN4 Antibody
Gene Names
NECTIN4; LNIR; PRR4; EDSS1; PVRL4; nectin-4
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
IF (Immunofluorescence)
(AAA31242 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31242 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Arc, Polyclonal Antibody (Cat# AAA31242)
Full Name
Arc Antibody
Gene Names
ARC; hArc; Arg3.1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Chicken(88%), Xenopus(83%)
Predicted Reactivity: Pig(100%), Bovine(100%), Chicken(88%), Xenopus(83%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31249 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31249 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Amino-terminal enhancer of split, Polyclonal Antibody (Cat# AAA31249)
Full Name
Amino-terminal enhancer of split Antibody
Gene Names
TLE5; AES; GRG; ESP1; GRG5; AES-1; AES-2; Grg-5
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Xenopus(86%)
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Xenopus(86%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31033 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CDC25B, Polyclonal Antibody (Cat# AAA31033)
Full Name
Phospho-CDC25B (Ser323) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunoflouorescence)
(AAA31011 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
ATF2, Polyclonal Antibody (Cat# AAA31011)
Full Name
Phospho-ATF2 (Thr69/51) Antibody
Gene Names
ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human lymph reacted with CD19 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
CD19, Polyclonal Antibody (Cat# AAA28685)
Full Name
CD19 Antibody (N-term)
Gene Names
CD19; B4; CVID3
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Immunohistochemistry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffinembedded mouse brain using KISS1R Antibody (AAA10717) at dilution of 1:100 (40x lens).)
KISS1R, Polyclonal Antibody (Cat# AAA10717)
Full Name
KISS1R Polyclonal Antibody
Gene Names
KISS1R; HH8; CPPB1; GPR54; AXOR12; KISS-1R; HOT7T175
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Pricing
IF (Immunofluorescence)
(AAA30956 staining HepG2 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA30956 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
MITF, Polyclonal Antibody (Cat# AAA30956)
Full Name
Phospho-MITF (Ser180/73) Antibody
Gene Names
MITF; MI; WS2; CMM8; WS2A; COMMAD; bHLHe32
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity puriification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin-embedded human breast cancer using KIF22 antibody (AAA28282) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol)
KIF22, Polyclonal Antibody (Cat# AAA28282)
Full Name
KIF22 Polyclonal Antibody
Gene Names
KIF22; KID; OBP; KNSL4; OBP-1; OBP-2; SEMDJL2; A-328A3.2
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using AAA29717 at dilution 1/5.)
CLEC7A, Polyclonal Antibody (Cat# AAA29717)
Full Name
CLEC7A Antibody
Gene Names
CLEC7A; BGR; CD369; CANDF4; DECTIN1; CLECSF12
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Pricing
IF (Immunofluorescence)
(AAA31156 staining HepG2 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31156 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
Beclin-1, Polyclonal Antibody (Cat# AAA31156)
Full Name
Phospho-Beclin-1 (Ser90/93/96) Antibody
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human, Mouse, Rat
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry-Paraffin)
(AAA31217 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
TBLR1, Polyclonal Antibody (Cat# AAA31217)
Full Name
TBLR1 Antibody
Gene Names
TBL1XR1; C21; DC42; IRA1; MRD41; TBLR1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of extracts from A431, using Phospho-SHP-2 (Tyr62) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)
SHP-2, Polyclonal Antibody (Cat# AAA31155)
Full Name
Phospho-SHP-2 (Tyr62) Antibody
Gene Names
PTPN11; CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3
Reactivity
Human, Mouse, Rat
Predicted: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Predicted: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(MUSK Antibody (AAA28697) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
MUSK, Polyclonal Antibody (Cat# AAA28697)
Full Name
MUSK Antibody
Gene Names
MUSK; CMS9
Reactivity
Human, mouse
Applications
Western Blot, Flow Cytometry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (PAB)
Pricing
FCM (Flow Cytometry)
(CCR7 Antibody (N-term) (AAA28701) flow cytometric analysis of 293 cells (right histogram) compared to a negative controlcell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
CCR7, Polyclonal Antibody (Cat# AAA28701)
Full Name
CCR7 Antibody (N-term)
Gene Names
CCR7; BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(LTF Antibody flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (lefthistogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for theanalysis.)
LTF, Polyclonal Antibody (Cat# AAA28680)
Full Name
LTF Antibody
Gene Names
LTF; LF; HLF2; GIG12; HEL110
Reactivity
Human, mouse
Applications
Western Blot, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA12069)
Full Name
RAT ANTI MOUSE CD8
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Reactivity
Mouse
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence
Pricing
WB (Western Blot)
(Western blot analysis of various lysates, using NACA Rabbit pAb (AAA28217) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.)
NACA, Antibody (Cat# AAA28217)
Full Name
NACA Polyclonal Antibody
Gene Names
NACA; HSD48; NACA1; skNAC; NAC-alpha
Applications
Western Blot
Purity
Affinity purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue AAA29660 using at dilution 1/40.)
PRKD1, Polyclonal Antibody (Cat# AAA29660)
Full Name
PRKD1 Antibody
Gene Names
PRKD1; PKD; PKCM; PRKCM; PKC-MU
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA28788 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28788, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was Rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
IDUA, Polyclonal Antibody (Cat# AAA28788)
Full Name
IDUA Antibody (Center)
Gene Names
IDUA; IDA; MPS1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western blot analysis of lysate from HT-29 cell line, using ERN2 Antibody (N-term). AAA28749 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)
ERN2, Polyclonal Antibody (Cat# AAA28749)
Full Name
ERN2 Antibody (N-term)
Gene Names
ERN2; IRE1b; IRE2p; hIRE2p; IRE1-BETA
Reactivity
Human
Applications
Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31243 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
ADCY10, Polyclonal Antibody (Cat# AAA31243)
Full Name
ADCY10 Antibody
Gene Names
ADCY10; SAC; HCA2; SACI; Sacy; hsAC; HEL-S-7a
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Bovine(88%), Sheep(88%), Rabbit(88%), Dog(88%)
Predicted Reactivity: Pig(88%), Bovine(88%), Sheep(88%), Rabbit(88%), Dog(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells with AAA26952 at 1:333, counter- stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
XKR8, Polyclonal Antibody (Cat# AAA26952)
Full Name
XKR8 Antibody
Gene Names
XKR8; XRG8; hXkr8
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
IHC (Immunohistochemistry)
(AAA31014 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Histone H2A.X, Polyclonal Antibody (Cat# AAA31014)
Full Name
Phospho-Histone H2A.X (Ser139) Antibody
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of FKHRL1 phosphorylation expression in serum treated NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
FKHRL1, Polyclonal Antibody (Cat# AAA30954)
Full Name
Phospho-FKHRL1 (Ser253) Antibody
Gene Names
FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31197 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31197 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
CXCL16, Polyclonal Antibody (Cat# AAA31197)
Full Name
CXCL16 Antibody
Gene Names
CXCL16; SRPSOX; CXCLG16; SR-PSOX
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistchemistry)
(AAA31110 at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
CHSY1, Polyclonal Antibody (Cat# AAA31110)
Full Name
CHSY1 Antibody
Gene Names
CHSY1; CHSY; CSS1; TPBS; ChSy-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistchemistry)
(AAA30928 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Nrf2, Polyclonal Antibody (Cat# AAA30928)
Full Name
Nrf2 Antibody
Gene Names
NFE2L2; NRF2; HEBP1; IMDDHH
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
Application Data
(The cancer cell lines were stably expressing DYKDDDDK-tagged RIP3 and RIP expression was detected by anti-RIP3 antibodies (AAA10922) in RIP3-overexpressed cells.)
RIP3, Polyclonal Antibody (Cat# AAA10922)
Full Name
RIP3 Antibody
Gene Names
Ripk3; Rip3; AW107945; 2610528K09Rik
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
RIP3 Antibody is affinity chromatography purified via peptide column.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human testis tissue using AAA26950 at dilution of 1:100)
FLVCR2, Polyclonal Antibody (Cat# AAA26950)
Full Name
FLVCR2 Antibody
Gene Names
FLVCR2; CCT; EPV; PVHH; MFSD7C; C14orf58; FLVCRL14q
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Pricing
IHC (Immunohistochemistry-Paraffin)
(Immunohistochemical analysis of paraffin-embedded H. liver section using TYK2 Antibody (C-term) . AAA28807 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
TYK2, Polyclonal Antibody (Cat# AAA28807)
Full Name
TYK2 Antibody (C-term)
Gene Names
TYK2; JTK1; IMD35
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of TLR2 in normal human prostate tissue using TLR2 antibody (Clone: ABM3A87) at 5 ug/ml.)
TLR2, Monoclonal Antibody (Cat# AAA14864)
Full Name
TLR2 Monoclonal Antibody
Gene Names
TLR2; TIL4; CD282
Reactivity
Mouse, Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot, Immunofluorescence
Purity
Protein G Chromatography
Pricing
WB (Western Blot)
(Western blot analysis of EGFR phosphorylation expression in 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
EGFR, Polyclonal Antibody (Cat# AAA30962)
Full Name
Phospho-EGFR (Ser695) Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(ERV3 Antibody (C-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ERV3 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.)
ERV3, Polyclonal Antibody (Cat# AAA28716)
Full Name
ERV3 Antibody (C-Term)
Gene Names
ERV3-1; ERV3; ERVR; envR; ERV-R; HERVR; HERV-R
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM (Flow Cytometry)
(Flow cytometry analysis of Crystallin alpha A in Balb/3T3 cells. The cell was stained with AAA11723 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
A crystallin A, Monoclonal Antibody (Cat# AAA11723)
Full Name
A crystallin A antibody
Gene Names
CRYAA; CRYA1; HSPB4; CTRCT9
Reactivity
Human
Applications
Western Blot, Flow Cytometry
Purity
By protein-G affinity chromatography
Pricing
IHC (Immunohistchemistry-Paraffin)
(AAA31276 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
SCAMP2, Polyclonal Antibody (Cat# AAA31276)
Full Name
SCAMP2 Antibody
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Predicted Reactivity: Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Collagen 4 alpha 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Collagen 4 alpha 5, Polyclonal Antibody (Cat# AAA27790)
Full Name
Anti-Collagen 4 alpha 5 Antibody
Gene Names
COL4A5; ATS; ASLN; CA54
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohistchemistry)
(Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.)
c-FOS, Antibody (Cat# AAA13673)
Full Name
Goat anti-c-FOS (aa283-295) Antibody
Gene Names
FOS; p55; AP-1; C-FOS
Reactivity
Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
Applications
Peptide ELISA, Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Pricing
WB (Western Blot)
(Immunofluorescent analysis of U251 cells, using LC3 Antibody (APG8B)(N-term). U251 cells(right) were treated with Chloroquine (50 uM,16h). AAA28778 was diluted at 1:25 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).)
LC3, Polyclonal Antibody (Cat# AAA28778)
Full Name
LC3 Antibody (APG8B) (N-term)
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, rat (Predicted Reactivity: Bovine)
Applications
Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western blot analysis of JunD phosphorylation expression in 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
JunD, Polyclonal Antibody (Cat# AAA31019)
Full Name
Phospho-JunD (Ser255) Antibody
Gene Names
JUND; AP-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31224 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31224 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
CASC5, Polyclonal Antibody (Cat# AAA31224)
Full Name
CASC5 Antibody
Gene Names
KNL1; D40; CT29; Spc7; CASC5; MCPH4; hKNL-1; AF15Q14; PPP1R55; hSpc105
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Horse(88%), Rabbit(86%)
Predicted Reactivity: Pig(88%), Horse(88%), Rabbit(86%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)
VPS35, Monoclonal Antibody (Cat# AAA27689)
Full Name
VPS35 Antibody, Clone 8A3: PerCP
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing
IF (Immunofluorescence)
(AAA29758 staining HepG2 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)
STING, Polyclonal Antibody (Cat# AAA29758)
Full Name
STING (Phospho-Ser366) Antibody
Gene Names
TMEM173; ERIS; MITA; MPYS; SAVI; NET23; STING; hMITA; hSTING
Reactivity
Human, Mouse
Applications
Westen Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of various lysates, using TIPARP Rabbit pAb (AAA28273) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
TIPARP, Polyclonal Antibody (Cat# AAA28273)
Full Name
TIPARP Polyclonal Antibody
Gene Names
TIPARP; PARP7; ARTD14; pART14
Applications
Western Blot
Purity
Affinity Purification
Pricing