Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
1718 results for " Cell Tissue Lysates" - showing 1550-1600
WB (Western Blot)
(Western blot analysis of extracts of various tissue sample, using Src Antibody.)
Src, Polyclonal Antibody (Cat# AAA31086)
Full Name
Src Antibody
Gene Names
SRC; ASV; SRC1; THC6; c-SRC; p60-Src
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(AAA31012 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
ATF2, Polyclonal Antibody (Cat# AAA31012)
Full Name
Phospho-ATF2 (Thr71/53) Antibody
Gene Names
ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Chk2 phosphorylation expression in UV treated COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Chk2, Polyclonal Antibody (Cat# AAA30958)
Full Name
Phospho-Chk2 (Thr383) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of SGK phosphorylation expression in Insulin treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
SGK, Polyclonal Antibody (Cat# AAA30942)
Full Name
Phospho-SGK (Ser422) Antibody
Gene Names
SGK1; SGK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA28750 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28750, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10, 000 events wasperformed.)
WNT5A, Polyclonal Antibody (Cat# AAA28750)
Full Name
WNT5A Antibody (Center)
Gene Names
WNT5A; hWNT5A
Reactivity
Human, Mouse, Rat
Predicted: Rabbit
Predicted: Rabbit
Applications
Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western blot analysis of EGFR phosphorylation expression in TSA treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
EGFR, Polyclonal Antibody (Cat# AAA30963)
Full Name
Phospho-EGFR (Ser1026) Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30920 at 1/200 staining human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Akt, Polyclonal Antibody (Cat# AAA30920)
Full Name
Phospho-Akt(Ser473) Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11930)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11864)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
Immunofluorescence
Pricing
FCM (Flow Cytometry)
(Intracellular flow cytometric analysis of RIG-I in K562 Cell line using 0.5 ug/10^6 cells of Anti-RIGI antibody (ABM40B5). Green represent isotype control and red represent Anti-RIG I antibody (AAA14865). Goat anti-mouse PE conjugate was used as secondary.)
RIG-I, Monoclonal Antibody (Cat# AAA14865)
Full Name
RIG-I Monoclonal Antibody
Gene Names
DDX58; RIGI; RIG-I; RLR-1; SGMRT2
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot
Purity
Protein G Chromatography
Pricing
IHC (Immunohistochemistry)
(AAA31136 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
PLK4, Polyclonal Antibody (Cat# AAA31136)
Full Name
PLK4 Antibody
Gene Names
PLK4; SAK; STK18; MCCRP2
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry
Purity
Peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IF (Immunofluorescence)
(AAA31031 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CDC25A, Polyclonal Antibody (Cat# AAA31031)
Full Name
Phospho-CDC25A (Ser124) Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(AAA31087 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
CREB, Polyclonal Antibody (Cat# AAA31087)
Full Name
CREB Antibody
Gene Names
CREB1; CREB; CREB-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
IHC (Immunohistchemistry)
(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
eIF2B epsilon, Polyclonal Antibody (Cat# AAA31414)
Full Name
Phospho-eIF2B epsilon (Ser540) Antibody
Gene Names
EIF2B5; CLE; CACH; LVWM; EIF-2B; EIF2Bepsilon
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (80%), Bovine (80%), Horse (80%), Rabbit (80%), Dog (80%), Chicken (80%)
Predicted Reactivity: Pig (80%), Bovine (80%), Horse (80%), Rabbit (80%), Dog (80%), Chicken (80%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(AAA31064 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
PKC theta, Polyclonal Antibody (Cat# AAA31064)
Full Name
Phospho-PKC theta (Ser676) Antibody
Gene Names
PRKCQ; PRKCT; nPKC-theta
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC-P (Immunohistochemistry Paraffin)
(AAA31041 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was use das the secondary antibody.)
STAT3, Polyclonal Antibody (Cat# AAA31041)
Full Name
Phospho-STAT3 (Tyr705) Antibody
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Peptide ELISA
Purity
Peptide affinity purification.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
CSK, Polyclonal Antibody (Cat# AAA31403)
Full Name
Phospho-CSK (Tyr304) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of AFX phosphorylation expression in serum treated 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
AFX, Polyclonal Antibody (Cat# AAA31062)
Full Name
Phospho-AFX (Ser197) Antibody
Gene Names
FOXO4; AFX; AFX1; MLLT7
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Vimentin, Polyclonal Antibody (Cat# AAA31419)
Full Name
Phospho-Vimentin (Ser39) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 488)
CD81, Monoclonal Antibody (Cat# AAA11942)
Full Name
HAMSTER ANTI MOUSE CD81
Gene Names
Cd81; Tapa1; Tapa-1; Tspan28
Reactivity
Rat
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12211)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of SHARP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
SHARP1, Polyclonal Antibody (Cat# AAA27795)
Full Name
Anti-SHARP1 Antibody
Gene Names
BHLHE41; DEC2; hDEC2; BHLHB3; SHARP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using AAA28679 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
HK1 (Hexokinase), Polyclonal Antibody (Cat# AAA28679)
Full Name
HK1 (Hexokinase) Antibody (N-term)
Gene Names
HK1; HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Jurkat cells, using CD3D Antibody.)
CD3D, Monoclonal Antibody (Cat# AAA30236)
Full Name
CD3D Antibody
Gene Names
CD3D; T3D; IMD19; CD3-DELTA
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
Affinity - chromatography.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with p70 S6 Kinase beta antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
p70S6 Kinase beta, Polyclonal Antibody (Cat# AAA29952)
Full Name
p70 S6 Kinase beta Antibody
Gene Names
RPS6KB2; KLS; SRK; S6K2; STK14B; p70S6Kb; P70-beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
Peptide affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with CD86 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
CD86, Monoclonal Antibody (Cat# AAA30048)
Full Name
CD86 Antibody
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Human, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
WB (Western Blot)
(All lanes : Anti-ERVK-7 Antibody (N-Term) at 1:1000-1:2000 dilutionLane 1: Raji whole cell lysateLane 2: human lung lysateLane 3: human testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 67 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
ERVK-7, Polyclonal Antibody (Cat# AAA28797)
Full Name
ERVK-7 Antibody (N-Term)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(AAA31032 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CDC25A, Polyclonal Antibody (Cat# AAA31032)
Full Name
Phospho-CDC25A (Thr507) Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Flow Cytometry detection of FOLR1 in MCF-7 human cell line using AAA14691. MCF-7 human breast cancer cell line was stained with AAA14691 (filled histogram) or isotype control antibody (open histogram), followed byPhycoerythrin-conjugated Anti-MouseIgG Secondary Antibody.)
FOLR1, Monoclonal Antibody (Cat# AAA14691)
Full Name
FOLR1 (Folate receptor 1 (adult) Adult folate-binding protein, FBP, Folate receptor, adult, Folate receptor 1, Folate receptor alpha, FOLR, FR-alpha, KB cells FBP, MOv18, Ovarian tumor-associated antigen MOv18)
Gene Names
FOLR1; FBP; FOLR
Reactivity
Human
Applications
Flow Cytometry, Western Blot, Immunocytochemistry
Purity
Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Pricing
IHC (Immunohistchemistry)
(AAA30947 at 1/100 staining rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Chk1, Polyclonal Antibody (Cat# AAA30947)
Full Name
Phospho-Chk1 (Ser301) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31029 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CDC25A, Polyclonal Antibody (Cat# AAA31029)
Full Name
Phospho-CDC25A (Ser75) Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30992 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
SP1, Polyclonal Antibody (Cat# AAA30992)
Full Name
Phospho-SP1 (Thr739) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various tissue sample, using Phospho-PLCG1 (Tyr771) Antibody.)
PLCG1, Polyclonal Antibody (Cat# AAA31021)
Full Name
Phospho-PLCG1 (Tyr771) Antibody
Gene Names
PLCG1; PLC1; NCKAP3; PLC-II; PLC148; PLCgamma1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30937 staining A-431 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
SYK, Polyclonal Antibody (Cat# AAA30937)
Full Name
Phospho-SYK (Tyr323) Antibody
Gene Names
SYK; p72-Syk
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30938 at 1/100 staining human muscle tumor tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
JAK1, Polyclonal Antibody (Cat# AAA30938)
Full Name
Phospho-JAK1 (Tyr1034/Tyr1035) Antibody
Gene Names
JAK1; JTK3; JAK1A; JAK1B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30945 at 1/100 staining rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Chk1, Polyclonal Antibody (Cat# AAA30945)
Full Name
Phospho-Chk1 (Ser296) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30953 at 1/100 staining rat ovarian tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
MAP3K7, Polyclonal Antibody (Cat# AAA30953)
Full Name
Phospho-MAP3K7 (Thr187) Antibody
Gene Names
MAP3K7; CSCF; FMD2; TAK1; MEKK7; TGF1a
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31043 at 1/200 staining Rat kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
STAT1, Polyclonal Antibody (Cat# AAA31043)
Full Name
Phospho-STAT1 (Ser727) Antibody
Gene Names
STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunoflouorescence)
(AAA31056 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
LIMK2, Polyclonal Antibody (Cat# AAA31056)
Full Name
Phospho-LIMK2 (Ser283) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(AAA31049 at 1/50 staining human colon cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Caspase 3, Polyclonal Antibody (Cat# AAA31049)
Full Name
Phospho-Caspase 3 (Ser150) Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30936 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
NF kappaB p65, Polyclonal Antibody (Cat# AAA30936)
Full Name
Phospho-NF kappaB p65 (Ser536) Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of FOXO4 phosphorylation expression in EGF treated HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
FOXO4, Polyclonal Antibody (Cat# AAA31061)
Full Name
Phospho-FOXO4 (Thr451) Antibody
Gene Names
FOXO4; AFX; AFX1; MLLT7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Ezrin phosphorylation expression in Na3VO4 treated K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Ezrin, Polyclonal Antibody (Cat# AAA31010)
Full Name
Phospho-Ezrin (Tyr478) Antibody
Gene Names
EZR; CVL; CVIL; VIL2; HEL-S-105
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31054 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
JNK1/2/3, Polyclonal Antibody (Cat# AAA31054)
Full Name
Phospho-JNK1/2/3 (Thr183+Tyr185) Antibody
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 antibody (N-term) (Cat.#AAA28756), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
EN1 (Engrailed 1), Polyclonal Antibody (Cat# AAA28756)
Full Name
EN1 (Engrailed 1) Antibody (N-term)
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(AAA30987 at 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
NPM, Polyclonal Antibody (Cat# AAA30987)
Full Name
Phospho-NPM (Thr199) Antibody
Gene Names
NPM1; B23; NPM
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CNTN1 Antibody at dilution 1/100, on the right is treated with synthetic peptide. (Original magnification: x200))
CNTN1, Polyclonal Antibody (Cat# AAA29730)
Full Name
CNTN1 Antibody
Gene Names
CNTN1; F3; GP135; MYPCN
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antigen affinity purification
Pricing
IHC (Immunohistochemistry)
(The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using AAA29759 (L1RE1 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200))
L1RE1, Polyclonal Antibody (Cat# AAA29759)
Full Name
L1RE1 Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Pricing
FCM (Flow Cytometry)
(ENAM Antibody (C-term) (AAA28699) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
ENAM, Polyclonal Antibody (Cat# AAA28699)
Full Name
ENAM Antibody (C-term)
Gene Names
ENAM; ADAI; AI1C; AIH2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Aquaporin 4 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Aquaporin 4, Monoclonal Antibody (Cat# AAA27803)
Full Name
Anti-Aquaporin 4 Antibody
Gene Names
AQP4; MIWC; WCH4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Pricing