Search results for "Yeast Antibody"

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ICC (Immunocytochemistry) (ICC staining SMC1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

SMC1, Monoclonal Antibody (Cat# AAA30184)

Full Name

SMC1 Antibody

Gene Names

SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IF, IHC

Purity

ProA affinity purified

Pricing

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WB (Western Blot) (WB Suggested Anti-FOXA1 antibody Titration: 1 ug/mLSample Type: Human liver)

FOXA1, Polyclonal Antibody (Cat# AAA23415)

Full Name

FOXA1 antibody - C-terminal region

Gene Names

FOXA1; HNF3A; TCF3A

Reactivity

Cow, Dog, Guinea Pig, Human, Mouse, Rat, Yeast, Zebrafish

Applications

WB, IHC

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ATOX1 on HeLa cell. [antibody concentration 10 ug/ml])

ATOX1, Monoclonal Antibody (Cat# AAA26078)

Full Name

ATOX1 (ATX1 Antioxidant Protein 1 Homolog (yeast), ATX1, HAH1, MGC138453, MGC138455) (APC)

Gene Names

ATOX1; ATX1; HAH1

Applications

IF, WB

Purity

Purified

Pricing

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WB (Western Blot) (TIMM9 monoclonal antibody. Western Blot analysis of TIMM9 expression in Raw 264.7.)

TIMM9, Monoclonal Antibody (Cat# AAA25568)

Full Name

TIMM9 (Mitochondrial Import Inner Membrane Translocase Subunit Tim9, TIM9, TIM9A, TIMM9A) (HRP)

Gene Names

TIMM9; TIM9; TIM9A

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3194).)

Prohibitin, Monoclonal Antibody (Cat# AAA23932)

Full Name

Prohibitin (Mitochondrial Marker)

Gene Names

PHB; PHB1; HEL-215; HEL-S-54e

Reactivity

Human

Applications

WB, IF, IHC

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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Application Data (Detection limit for recombinant GST tagged CSE1L is approximately 3ng/ml as a capture antibody.)

CSE1L, Monoclonal Antibody (Cat# AAA26056)

Full Name

CSE1L (CSE1 Chromosome Segregation 1-like (yeast), CAS, CSE1, MGC117283, MGC130036, MGC130037, XPO2) (APC)

Gene Names

CSE1L; CAS; CSE1; XPO2

Applications

IF, IHC, WB

Purity

Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ATOX1 on HeLa cell. [antibody concentration 10 ug/ml])

ATOX1, Monoclonal Antibody (Cat# AAA26609)

Full Name

ATOX1 (ATX1 Antioxidant Protein 1 Homolog (yeast), ATX1, HAH1, MGC138453, MGC138455) (PE)

Gene Names

ATOX1; ATX1; HAH1

Applications

IF, WB

Purity

Purified

Pricing

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WB (Western Blot) (TIMM9 monoclonal antibody. Western Blot analysis of TIMM9 expression in Raw 264.7.)

TIMM9, Monoclonal Antibody (Cat# AAA24979)

Full Name

TIMM9 (Mitochondrial Import Inner Membrane Translocase Subunit Tim9, TIM9, TIM9A, TIMM9A) (Biotin)

Gene Names

TIMM9; TIM9; TIM9A

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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eRF1/ETF1, Monoclonal Antibody (Cat# AAA19382)

Full Name

Anti-eRF1/ETF1 Antibody (monoclonal, 3B6)

Gene Names

ETF1; ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)

Hsp70/Hsc70, Monoclonal Antibody (Cat# AAA17795)

Full Name

Hsp70/Hsc70 Antibody: RPE

Gene Names

HSPA2; HSP70

Reactivity

Human, Mouse, Rat, Sheep, Dog, Beluga, Bovine, Fish, Guinea pig, Scallop pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast

Applications

WB, IP, IHC

Pricing

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WB (Western Blot) (Western Blot analysis of TOMM22 expression in transfected 293T cell line by TOMM22 monoclonal antibody. Lane 1: TOMM22 transfected lysate (15.5kD). Lane 2: Non-transfected lysate.)

TOMM22, Monoclonal Antibody (Cat# AAA25277)

Full Name

TOMM22 (Translocase of Outer Membrane 22kD Subunit Homolog, Mitochondrial Import Receptor Subunit TOM22 Homolog, TOM22, hTom22, 1C9-2, MST065, MSTP065) (FITC)

Gene Names

TOMM22; 1C9-2; TOM22; MST065; MSTP065

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (BUB1B monoclonal antibody Western Blot analysis of BUB1B expression in Hela NE.)

BUBR1, Monoclonal Antibody (Cat# AAA25036)

Full Name

BUBR1 (Mitotic Checkpoint Serine/Threonine-protein Kinase BUB1 beta, MAD3/BUB1-related Protein Kinase, hBUBR1, Mitotic Checkpoint Kinase MAD3L, Protein SSK1, BUB1B, MAD3L, SSK1) (FITC)

Gene Names

BUB1B; MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (WB Suggested Anti-SDHB Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysateSDHB is supported by BioGPS gene expression data to be expressed in Jurkat)

SDHB, Polyclonal Antibody (Cat# AAA23528)

Full Name

SDHB antibody - middle region

Gene Names

SDHB; IP; SDH; CWS2; PGL4; SDH1; SDH2; SDHIP

Reactivity

Tested: Human
Predicted Reactivity: Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish

Applications

WB

Purity

Affinity Purified

Pricing

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WB (Western Blot) (WB Suggested Anti-RPS14 Antibody Titration: 2.5ug/mlELISA Titer: 1:12500Positive Control: HepG2 cell lysate)

RPS14, Polyclonal Antibody (Cat# AAA23472)

Full Name

RPS14 antibody - middle region

Gene Names

RPS14; S14; EMTB

Reactivity

Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish

Applications

WB

Purity

Protein A purified

Pricing

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WB (Western Blot) (WB Suggested Anti-THOC1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: HepG2 cell lysate)

THOC1, Polyclonal Antibody (Cat# AAA23479)

Full Name

THOC1 Antibody - C-terminal region

Gene Names

THOC1; P84; HPR1; P84N5

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Yeast, Zebrafish

Applications

IHC, WB

Purity

Affinity Purified

Pricing

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TOMM20 on HeLa cell. [antibody concentration 10ug/ml])

TOMM20, Monoclonal Antibody (Cat# AAA24980)

Full Name

TOMM20 (Mitochondrial Import Receptor Subunit TOM20 Homolog, TOM20, Mitochondrial 20kD Outer Membrane Protein, Outer Mitochondrial Membrane Receptor Tom20, KIAA0016, MAS20, MOM19)

Gene Names

TOMM20; MAS20; MOM19; TOM20

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

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WB (Western Blot) (Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3225).)

Prohibitin, Monoclonal Antibody (Cat# AAA23933)

Full Name

Prohibitin (Mitochondrial Marker)

Gene Names

PHB; PHB1; HEL-215; HEL-S-54e

Reactivity

Human

Applications

WB, IF, IHC

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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TOMM40, Polyclonal Antibody (Cat# AAA10745)

Full Name

TOMM40 Polyclonal Antibody

Gene Names

TOMM40; TOM40; PEREC1; C19orf1; PER-EC1; D19S1177E

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity Purification

Pricing

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IHC (Immunohistochemistry) (EIF2S1 Antibody (N-term) (Cat. #AAA28744)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EIF2S1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

EIF2S1, Polyclonal Antibody (Cat# AAA28744)

Full Name

EIF2S1 Antibody (N-term)

Gene Names

EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha

Reactivity

Mouse (Predicted Reactivity: Zebrafish, Bovine, Chicken, Drosophila, Pig, Rat, Yeast)

Applications

Western Blot, Immunofluorescence, Immunohistochemistry

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

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DECTIN-1, Monoclonal Antibody (Cat# AAA12129)

Full Name

RAT ANTI MOUSE DECTIN-1:Low Endotoxin

Gene Names

Clec7a; BGR; beta-GR; Clecsf12

Applications

Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation

Pricing

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TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12010)

Full Name

RAT ANTI TUBULIN ALPHA

Applications

Immunohistochemistry, Immunofluorescence, Radioimmunoassay, Western Blot

Pricing

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DECTIN-1, Monoclonal Antibody (Cat# AAA12130)

Full Name

RAT ANTI MOUSE DECTIN-1:FITC

Gene Names

Clec7a; BGR; beta-GR; Clecsf12

Applications

Flow Cytometry

Pricing

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DECTIN-1, Monoclonal Antibody (Cat# AAA12136)

Full Name

RAT ANTI MOUSE DECTIN-1:RPE

Gene Names

Clec7a; BGR; beta-GR; Clecsf12

Applications

Flow Cytometry

Pricing

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DECTIN-1, Monoclonal Antibody (Cat# AAA12131)

Full Name

RAT ANTI MOUSE DECTIN-1:FITC

Gene Names

Clec7a; BGR; beta-GR; Clecsf12

Applications

Flow Cytometry

Pricing

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DECTIN-1, Monoclonal Antibody (Cat# AAA12133)

Full Name

RAT ANTI MOUSE DECTIN-1:FITC

Gene Names

Clec7a; BGR; beta-GR; Clecsf12

Applications

Flow Cytometry

Pricing

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DECTIN-1, Monoclonal Antibody (Cat# AAA12135)

Full Name

RAT ANTI MOUSE DECTIN-1:RPE

Gene Names

Clec7a; BGR; beta-GR; Clecsf12

Applications

Flow Cytometry

Pricing

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DECTIN-1, Monoclonal Antibody (Cat# AAA12126)

Full Name

RAT ANTI MOUSE DECTIN-1

Gene Names

Clec7a; BGR; beta-GR; Clecsf12

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Pricing

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DECTIN-1, Monoclonal Antibody (Cat# AAA12134)

Full Name

RAT ANTI MOUSE DECTIN-1

Gene Names

Clec7a; BGR; beta-GR; Clecsf12

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Pricing

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IHC (Immunohistchemistry) (Fig.6. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.)

beta-Tubulin, Monoclonal Antibody (Cat# AAA31540)

Full Name

Anti-beta-Tubulin Mouse Monoclonal Antibody (3G6)

Gene Names

TUBB3; CDCBM; TUBB4; beta-4; CFEOM3A

Reactivity

Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Sheep, Insect, Yeast, Hamster

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Pricing

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Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

Application Data (Published customer image: Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A') The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A'). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 um; D-F, I-K, N-P: 200 um; Q: 100 um; R, S: 10 um; T: 50 um; U, V, W: 25 um.From: Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. Dayraud, CC. et al. BMC Evolutionary Biology 2012, 12:107.)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12232)

Full Name

RAT ANTI TUBULIN ALPHA:HRP

Applications

Immunohistochemistry, Western Blot

Pricing

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Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

Application Data (Published customer image: Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A') The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A'). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 um; D-F, I-K, N-P: 200 um; Q: 100 um; R, S: 10 um; T: 50 um; U, V, W: 25 um.From: Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. Dayraud, CC. et al. BMC Evolutionary Biology 2012, 12:107.)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12009)

Full Name

RAT ANTI TUBULIN ALPHA

Applications

Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western Blot

Pricing

HBcAg, Recombinant Protein (Cat# AAA14357)

Full Name

HBcAg protein

Applications

Western Blot

Purity

>95 % (by SDS-PAGE)

Pricing

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WB (Western Blot) (Fig.8 Western blot analysis of Hela, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25°C, 3h).)

GAPDH, Monoclonal Antibody (Cat# AAA31539)

Full Name

Anti-GAPDH Mouse Monoclonal Antibody (2B5)

Gene Names

GAPDH; G3PD; GAPD

Reactivity

Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Pig, Sheep, Yeast, Hamster

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Pricing

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IF (Immunofluorescence) (Fig.7. Immunofluorescence analysis of rat liver tissue. 1, Histone H3 Monoclonal Antibody (2D10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)

Histone H3, Monoclonal Antibody (Cat# AAA31543)

Full Name

Anti-Histone H3 Mouse Monoclonal Antibody (2D10)

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat, Yeast

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Pricing

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IF (Immunofluorescence) (AAA31230 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31230 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)