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Application Data (Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)

IFN GAMMA, Monoclonal Antibody (Cat# AAA12096)

Full Name

MOUSE ANTI BOVINE INTERFERON GAMMA:RPE

Applications

Flow Cytometry

Pricing

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CD81, Monoclonal Antibody (Cat# AAA12033)

Full Name

HAMSTER ANTI MOUSE CD81:RPE

Gene Names

Cd81; Tapa1; Tapa-1; Tspan28

Applications

Flow Cytometry

Pricing

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Application Data (PE conjugatedMouse anti Human CD169 antibody, clone 7-239 used to block CD169 function on myeloid cells.Image caption:Siglec-1 mediates HIV-1 uptake into a storage compartment and enhances HIV-1 trans-infection specially in IFN?-treated monocytes and DCs. A. Uptake of HIV-1NL4–3 by different myeloid cells exposed to IFN?. Cells were cultured with HIV-1 to measure p24Gag by ELISA. Mean values and SEM from four experiments include cells from 12 donors. B. Fold change in HIV-1NL4–3 uptake of cells treated with bafilomycin A1 compared to untreated cells. Mean values and SEM include cells from three donors. C. Relative uptake of HIV-1NL4–3 by IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 uptake by mock-treated cells (set at 100%). Mean values and SEM from two experiments include cells from six donors. D. Confocal microscopy analysis of different IFN?-treated myeloid cells pulsed with HIV-1Cherry and stained for Siglec-1 (Alexa 488), HLA-DR (Alexa 647) and DAPI. (Top) Representative viral pattern for each kind of myeloid cell analyzed, showing maximum fluorescence intensity of four channels. (Bottom) Percentage of myeloid cells with distinct viral patterns: random distribution, polarized accumulation, and sac-like compartment formation, as illustrated in the left drawing. Mean values of 50 cells from two different donors are shown. E. HIV-1 transmission from IFN?-treated myeloid cells to a luciferase reporter CD4+ cell line. HIV-1 infection was determined by induced luciferase activity in relative light units (RLUs). Mean values and SEM from four experiments include cells from 12 donors. F. Relative HIV-1 transmission from IFN?-treated myeloid cells pre-incubated with the indicated mAbs. Values are normalized to the level of HIV-1 trans-infected by mock-treated cells. Mean values and SEM from two experiments include cells from six donors. Statistical differences were assessed with a paired t test in A and E, and with a one sample t-test in B, C and F.From: Pino M, Erkizia I, Benet S, Erikson E, Fernández-Figueras MT, Guerrero D, Dalmau J, Ouchi D, Rausell A, Ciuffi A, Keppler OT, Telenti A, Kräusslich HG, Martinez-Picado J, Izquierdo-Useros N.HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells.Retrovirology. 2015 May 7;12:37.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data (PE conjugated Mouse anti CD169 antibody, clone 7-239 used for the evaluation of CD169 expression on myeloid cell populations by flow cytometry. Mouse anti Human CD169 antibody, clone 7-239 used to block CD169 function on myeliod cells.Image caption:Siglec-1 mediates HIV-1 capture by IFN?-treated myeloid cells. A. Representative profiles of Siglec-1 staining in distinct myeloid cells cultured with or without 1000 U/ml of IFN? and assessed by FACS. Staining of matched-isotype control is also shown. The mean fold increase in fluorescence after IFN&apha; treatment of cells derived from three donors is shown in red numbers. B. Mean number of Siglec-1 antibody binding sites per cell displayed by different myeloid cells exposed to 1000 U/ml of IFN? for 48 h and assessed by quantitative FACS analysis. Data show mean values and SEM from four experiments including cells from 12 donors. C. Comparative binding of HIV-1NL4–3 to different myeloid cells ly exposed to 1000 U/ml of IFN? for 48 h. Cells were cultured with HIV-1NL4–3 for 4 h at 4°C, washed and lysed to measure p24Gag by ELISA. Data show mean values and SEM from two experiments including cells from six donors. D. Relative binding of HIV-1NL4–3 by different IFN?-treated myeloid cells that had been pre-incubated with 10 ug/ml of the indicated mAbs before HIV-1 exposure for 4 h at 4°C as described in C. To compare the effect of the mAbs in different myeloid cells, values were normalized to the level of HIV-1 binding by mock-treated cells (set to 100%). Data show mean values and SEM from two experiments including cells from six donors. Statistical differences were assessed with a paired t test in B and C, and with a one sample t-test in D.From: Pino M, Erkizia I, Benet S, Erikson E, Fernández-Figueras MT, Guerrero D, Dalmau J, Ouchi D, Rausell A, Ciuffi A, Keppler OT, Telenti A, Kräusslich HG, Martinez-Picado J, Izquierdo-Useros N.HIV-1 immune activation induces Siglec-1 expression and enhances viral trans-infection in blood and tissue myeloid cells.Retrovirology. 2015 May 7;12:37.This image is from an open access article distributed under the terms of the Creative Commons Attribution License.)

CD169, Monoclonal Antibody (Cat# AAA12265)

Full Name

Mouse Anti Human CD169: RPE

Gene Names

SIGLEC1; SN; CD169; SIGLEC-1

Reactivity

Human

Applications

Flow Cytometry

Purity

>95% by SDS PAGE
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.

Pricing

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CD81, Monoclonal Antibody (Cat# AAA11869)

Full Name

HAMSTER ANTI MOUSE CD81:FITC

Gene Names

Cd81; Tapa1; Tapa-1; Tspan28

Applications

Flow Cytometry

Pricing

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RECA-1, Monoclonal Antibody (Cat# AAA12019)

Full Name

MOUSE ANTI RAT RECA-1

Applications

Immunohistochemistry, Immunofluorescence

Pricing

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CD163, Monoclonal Antibody (Cat# AAA11943)

Full Name

MOUSE ANTI HUMAN CD163

Gene Names

CD163; M130; MM130

Reactivity

Guinea Pig, Pig, Rhesus Monkey, Sheep

Applications

Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoassay, Western Blot

Pricing

HA (B/Phuket/3073/2013), Monoclonal Antibody (Cat# AAA13690)

Full Name

Anti-HA (B/Phuket/3073/2013) Monoclonal Antibody

Applications

Western Blot, Immunoprecipitation, Immunofluorescence

Pricing

P.falciparum pLDH, Recombinant Protein (Cat# AAA13454)

Full Name

P.falciparum pLDH, Recomb.

Applications

Lateral Flow

Purity

> 95% pure (SDS-PAGE), Immobilized metal affinity chromatography (IMAC) using Ni (II)-NTA.

Pricing

PAI-1, Monoclonal Antibody (Cat# AAA27728)

Full Name

Anti-PAI-1 Antibody, Mouse Monoclonal

Gene Names

SERPINE1; PAI; PAI1; PAI-1; PLANH1

Applications

ELISA

Purity

Protein A affinity chromatography.

Pricing

p. VIVAX Pldh, Recombinant Protein (Cat# AAA13455)

Full Name

p. VIVAX Pldh, Recomb.

Applications

Lateral Flow, Western Blot

Purity

> 95% pure (SDS-PAGE), Immobilized metal affinity chromatography (IMAC) using Ni (II)-NTA.

Pricing

PPME1, Recombinant Protein (Cat# AAA11812)

Full Name

PPME1, 1-386aa, Human, His tag, E Coli

Gene Names

PPME1; PME-1

Applications

SDS-PAGE

Purity

> 95% by SDS-PAGE

Pricing

NCOR1, Monoclonal Antibody (Cat# AAA14104)

Full Name

Anti-NCOR1 Mouse mAb

Gene Names

NCOR1; N-CoR; TRAC1; N-CoR1; hN-CoR; PPP1R109

Reactivity

Human

Applications

Immunohistochemistry

Purity

Ascetic Fluid

Pricing

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CX43, Polyclonal Antibody (Cat# AAA13870)

Full Name

Connexin 43 Polyclonal Antibody

Gene Names

cx43; gja1; cx43a1; zfCx43.3

Reactivity

Reacts against human, rat, mouse, canine and monkey proteins

Applications

Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

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Asporin, Recombinant Protein (Cat# AAA20992)

Full Name

Recombinant Asporin (ASPN)

Gene Names

ASPN; OS3; PLAP1; PLAP-1; SLRR1C

Reactivity

Mus musculus (Mouse)

Applications

Western Blot

Purity

> 95%

Pricing

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IF (Immunofluorescence) (AAA31212 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31212 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

PAPP A2, Polyclonal Antibody (Cat# AAA31212)

Full Name

PAPP A2 Antibody

Gene Names

PAPPA2; PAPPE; PLAC3; PAPP-E; PAPP-A2

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Sheep(80%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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CD1a, Monoclonal Antibody (Cat# AAA11890)

Full Name

MOUSE ANTI HUMAN CD1a:FITC

Gene Names

CD1A; R4; T6; CD1; FCB6; HTA1

Applications

Flow Cytometry

Pricing

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Application Data (Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)

Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12188)

Full Name

RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:Biotin

Applications

Flow Cytometry

Pricing

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Application Data (Analysis of Protein Array containing >19,000 full-length human proteins using MSH6 Mouse Monoclonal Antibody (MSH6/3086) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

MSH6, Monoclonal Antibody (Cat# AAA23917)

Full Name

MSH6 (DNA Mismatch Repair Protein)

Gene Names

MSH6; GTBP; HSAP; p160; GTMBP; HNPCC5

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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FCM (Flow Cytometry) (Figure 5. Flow Cytometry analysis of RH35 cells using anti-Podoplanin/gp36/Pdpn antibody (AAA19233).Overlay histogram showing RH35 cells stained with AAA19233 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Podoplanin/gp36/Pdpn Antibody (AAA19233, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Podoplanin/gp36/Pdpn, Polyclonal Antibody (Cat# AAA19233)

Full Name

Anti-Podoplanin/gp36/Pdpn Antibody

Gene Names

Pdpn; T1a; Gp38; OTS-8; T1alpha; RANDAM-2; T1-alpha

Reactivity

Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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CD8 ALPHA, Monoclonal Antibody (Cat# AAA11981)

Full Name

MOUSE ANTI RAT CD8 ALPHA

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot

Pricing

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CD105, Monoclonal Antibody (Cat# AAA12084)

Full Name

MOUSE ANTI HUMAN CD105:RPE

Gene Names

ENG; END; HHT1; ORW1

Applications

Flow Cytometry

Pricing

E (DIII) (WNV), Recombinant Protein (Cat# AAA13741)

Full Name

West Nile Virus E (DIII) Protein

Applications

Western Blot

Purity

> 95% purity (SDS-PAGE)

Pricing

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Cre, Polyclonal Antibody (Cat# AAA13864)

Full Name

Cre Recombinase Polyclonal Antibody

Reactivity

Bacteriophage P1

Applications

Western Blot

Pricing

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IHC (Immunohistochemistry) (IHC(P):Rat Spleen Tissue)

FOXP3, Polyclonal Antibody (Cat# AAA11651)

Full Name

Anti-FOXP3 Antibody

Gene Names

FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Immunogen Affinity Purified

Pricing

ACO1, Recombinant Protein (Cat# AAA11797)

Full Name

ACO1, 1-889aa, Human, His tag, E Coli

Gene Names

ACO1; IRP1; ACONS; HEL60; IREB1; IREBP; IREBP1

Applications

SDS-PAGE

Purity

> 90% by SDS-PAGE

Pricing

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FCM (Flow Cytometry) (Flow cytometry analysis of PARP2 in U87MG cells. The cell was stained with AAA11732 at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black))

PARP2, Monoclonal Antibody (Cat# AAA11732)

Full Name

PARP2 antibody

Gene Names

PARP2; ARTD2; ADPRT2; PARP-2; ADPRTL2; ADPRTL3; pADPRT-2

Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence

Purity

By protein-A affinity chromatography

Pricing

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Application Data (Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)

Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12196)

Full Name

RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

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Application Data (Staining of New Zealand Black mouse peripheral blood granulocytes with Rat anti Mouse Ly-6B.2 conjugated to FITC Data)

Ly-6B.2 ALLOANTIGEN, Monoclonal Antibody (Cat# AAA12199)

Full Name

RAT ANTI MOUSE Ly-6B.2 ALLOANTIGEN:RPE

Applications

Flow Cytometry

Pricing

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A549 cells using anti-MDR3 antibody (AAA11652).Overlay histogram showing A549 cells stained with AAA11652 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MDR3 Antibody (AAA11652,1ug/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ABCB4, Polyclonal Antibody (Cat# AAA11652)

Full Name

Anti-ABCB4 Antibody

Gene Names

ABCB4; GBD1; ICP3; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen Affinity Purified

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of 293T cells using anti-ARG2 antibody (AAA19256).Overlay histogram showing 293T cells stained with AAA19256 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARG2 Antibody (AAA19256, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ARG2, Polyclonal Antibody (Cat# AAA19256)

Full Name

Anti-ARG2 Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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CD105, Monoclonal Antibody (Cat# AAA11933)

Full Name

MOUSE ANTI HUMAN CD105

Gene Names

ENG; END; HHT1; ORW1

Reactivity

Cynomolgus monkey, Horse, Monkey, Primate, Rhesus Monkey

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot

Pricing

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Application Data (Published customer image: gamma delta T cells are the primary source of IL-17 during B. abortus infection. C57BL/6 mice were infected i.p. with 5x104 CFUs of B. abortus 2308, and two weeks later gamma delta T cells (>95% purity) and an enriched TCRalphabeta (~55% CD4+, 25% CD8+) cell fraction were isolated from the spleens of infected mice. Cells were stimulated with 500 ng/ml ionomycin and 50 ng/ml PMA for three days, and cell-free supernatants from triplicate wells were assayed for cytokine production via ELISA. The mean +/- SD is shown; * P)

IFN GAMMA, Monoclonal Antibody (Cat# AAA12094)

Full Name

MOUSE ANTI BOVINE INTERFERON GAMMA:Biotin

Applications

Flow Cytometry

Pricing

SCCA1, Recombinant Protein (Cat# AAA13527)

Full Name

SCCA1 Protein (Active)

Applications

ELISA, SDS-PAGE

Purity

>95% by SDS-PAGE

Pricing

HIV-1 tat, Recombinant Protein (Cat# AAA14180)

Full Name

HIV-1 TAT antigen (recombinant) full length 14 kDa

Purity

> 95% pure
Purity of proteins is evaluated by SDS-PAGE by measuring optimal density at 280 nm.

Pricing

gp120/160 (SIV/mac239), Polyclonal Antibody (Cat# AAA13713)

Full Name

Anti-gp120/160 (SIV-1/mac239) Rabbit Polyclonal Antibody

Applications

Western Blot

Purity

Immunoaffinity chromatography

Pricing

CLC, Recombinant Protein (Cat# AAA11808)

Full Name

CLC, 1-142aa, Human, His tag, E Coli

Gene Names

CLC; GAL10; Gal-10; LGALS10; LGALS10A; LPPL_HUMAN

Applications

SDS-PAGE

Purity

> 90% by SDS-PAGE

Pricing

HA HRP, Polyclonal Antibody (Cat# AAA11721)

Full Name

HA Affinity Purified Antibody, HRP Conjugated

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Antibody was purified by peptide affinity chromatography and conjugated to horseradish peroxidase (HRP).

Pricing

GroEL, Recombinant Protein (Cat# AAA11737)

Full Name

GroEL, 1-548aa, E Coli, E Coli

Gene Names

groL; ECK4137; groEL; JW4103; mopA

Applications

SDS-PAGE

Purity

> 95% by SDS-PAGE

Pricing

FGF2, Polyclonal Antibody (Cat# AAA11513)

Full Name

Anti-human FGF2 antibody

Gene Names

FGF2; BFGF; FGFB; FGF-2; HBGF-2

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Human placenta tissue using AAA28695 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)