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Application
Clone
2304 results for "Secondary Antibody" - showing 1050-1100
IHC (Immunohistochemistry)
(At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Smad5, Polyclonal Antibody (Cat# AAA31331)
Full Name
Phospho-Smad5 (Ser463/Ser465) Antibody
Gene Names
SMAD5; DWFC; JV5-1; MADH5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of MCF-7 cells using anti- PCK2 antibody (AAA19384).Overlay histogram showing MCF-7 cells stained with AAA19384 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PCK2 Antibody (AAA19384, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PCK2, Monoclonal Antibody (Cat# AAA19384)
Full Name
Anti-PCK2 Antibody (monoclonal, 3F7)
Gene Names
PCK2; PEPCK; PEPCK2; PEPCK-M
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of HL-60 cells using anti-NDUFB10 antibody (AAA19339).Overlay histogram showing HL-60 cells stained with AAA19339 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NDUFB10 Antibody (AAA19339, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
NDUFB10, Polyclonal Antibody (Cat# AAA19339)
Full Name
Anti-NDUFB10 Antibody
Gene Names
NDUFB10; PDSW
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using GLUD2 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
GLUD2, Polyclonal Antibody (Cat# AAA28149)
Full Name
GLUD2 Polyclonal Antibody
Gene Names
GLUD2; GDH2; GLUDP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Hela cells using AAA26918 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
NOL4L, Polyclonal Antibody (Cat# AAA26918)
Full Name
NOL4L Antibody
Gene Names
NOL4L; C20orf112; C20orf113; dJ1184F4.2; dJ1184F4.4
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
>95%,Protein G purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Zyxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Zyxin, Monoclonal Antibody (Cat# AAA30399)
Full Name
Zyxin Antibody
Gene Names
ZYX; ESP-2; HED-2
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Cortactin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Cortactin, Monoclonal Antibody (Cat# AAA30148)
Full Name
Cortactin Antibody
Gene Names
CTTN; EMS1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of CRM1 using anti-CRM1 antibody (AAA11673).CRM1 was detected in frozen section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CRM1 Antibody (AAA11673) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
CRM1, Polyclonal Antibody (Cat# AAA11673)
Full Name
Anti-CRM1 Antibody
Gene Names
XPO1; emb; CRM1; exp1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-AML1 (Ser397) in lysates of Jurkat, using Phospho-AML1 (Ser397) Antibody.)
AML1, Polyclonal Antibody (Cat# AAA31153)
Full Name
Phospho-AML1 (Ser397) Antibody
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Predicted: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Figure 5. IHC analysis of GNG4 using anti-GNG4 antibody (AAA19341).GNG4 was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GNG4 Antibody (AAA19341) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)
GNG4, Polyclonal Antibody (Cat# AAA19341)
Full Name
Anti-GNG4 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-CHRNA5 antibody (AAA11685).Overlay histogram showing MCF-7 cells stained with AAA11685 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CHRNA5 Antibody (AAA11685,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CHRNA5, Polyclonal Antibody (Cat# AAA11685)
Full Name
Anti-CHRNA5 Antibody
Gene Names
CHRNA5; LNCR2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of A431 cells using anti-UBE2D1/2/3/4 antibody (AAA19298).Overlay histogram showing A431 cells stained with AAA19298 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-UBE2D1/2/3/4 Antibody (AAA19298, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
UBE2D1/2/3/4, Polyclonal Antibody (Cat# AAA19298)
Full Name
Anti-UBE2D1/2/3/4 Antibody
Gene Names
UBE2D1; SFT; UBCH5; UBC4/5; UBCH5A; E2(17)KB1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of THP-1 cells using anti-Ku70 antibody (AAA19362).Overlay histogram showing THP-1 cells stained with AAA19362 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Ku70 Antibody (AAA19362, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Ku70, Monoclonal Antibody (Cat# AAA19362)
Full Name
Anti-Ku70 Antibody (monoclonal, 3D7)
Gene Names
XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Positive WB detected in: recombinant proteinAll lanes:mpt64 Antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 26 kDaObserved band size: 26 kDa)
Mycobacterium tuberculosis Immunogenic protein MPT64, Polyclonal Antibody (Cat# AAA15950)
Full Name
Rabbit anti-Mycobacterium tuberculosis Immunogenic protein MPT64 polyclonal Antibody
Gene Names
mpt64; mpb64
Reactivity
Mycobacterium tuberculosis
Applications
EIA, WB
Purity
>95%, Protein G purified
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using CETN3 antibody at dilution of 1:100 (40x lens).)
CETN3, Polyclonal Antibody (Cat# AAA28193)
Full Name
CETN3 Polyclonal Antibody
Gene Names
CETN3; CEN3; CDC31
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of LOVO cells with Wnt2b antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Wnt2b, Monoclonal Antibody (Cat# AAA30442)
Full Name
Wnt2b Antibody
Gene Names
WNT2B; WNT13
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
ProA affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of paraffin-embedded mouse spleen using LILRB4 Rabbit pAb (AAA28090) at dilution of 1:100 (40x lens).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.)
LILRB4, Antibody (Cat# AAA28090)
Full Name
LILRB4
Gene Names
LILRB4; ILT3; LIR5; CD85K; ILT-3; LIR-5
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY-5Y cells with PSD95 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
PSD95, Monoclonal Antibody (Cat# AAA29987)
Full Name
PSD95 Antibody
Gene Names
DLG4; PSD95; SAP90; SAP-90
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
WB (Western Blot)
(Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.)
Hsp90, Monoclonal Antibody (Cat# AAA17788)
Full Name
Hsp90 Antibody: PerCP
Gene Names
HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B
Reactivity
Human (beta-specific), Rabbit (Beta Specific), Chicken (Alpha/Beta), Rat, Canine
Applications
WB, IP, EIA, IHC, IF
Pricing
IHC (Immunohistochemistry)
(Figure 8. IHC analysis of DDB1 using anti-DDB1 antibody (AAA11660).DDB1 was detected in frozen section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DDB1 Antibody (AAA11660) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
DDB1, Polyclonal Antibody (Cat# AAA11660)
Full Name
Anti-DDB1 Antibody
Gene Names
DDB1; XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
Application Data
(Analysis of Protein Array containing >19, 000 full-length human proteins using EpCAM Mouse Monoclonal Antibody (EGP40/1372) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
Ep-CAM/CD326, Monoclonal Antibody (Cat# AAA23890)
Full Name
Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker)
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human. Others not known.
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
FRS2, Polyclonal Antibody (Cat# AAA31412)
Full Name
Phospho-FRS2 (Tyr196) Antibody
Gene Names
FRS2; SNT; SNT1; FRS2A; SNT-1; FRS2alpha
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(Figure 6. IHC analysis of DYNLT1 using anti-DYNLT1 antibody (AAA19175).DYNLT1 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DYNLT1 Antibody (AAA19175) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
DYNLT1, Polyclonal Antibody (Cat# AAA19175)
Full Name
Anti-DYNLT1 Picoband antibody
Gene Names
DYNLT1; CW-1; TCTEL1; tctex-1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using ALDH1A1 antibody at dilution of 1:100 (40x lens).)
ALDH1A1, Polyclonal Antibody (Cat# AAA10725)
Full Name
ALDH1A1 Polyclonal Antibody
Gene Names
ALDH1A1; ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human esophagus using GTF2I antibody (40x lens).)
GTF2I, Polyclonal Antibody (Cat# AAA28199)
Full Name
GTF2I Polyclonal Antibody
Gene Names
GTF2I; WBS; DIWS; SPIN; IB291; BAP135; BTKAP1; TFII-I; WBSCR6; GTFII-I
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
WB (Western Blot)
(WB Suggested Anti-RCAN1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: Human Muscle)
RCAN1, Polyclonal Antibody (Cat# AAA23463)
Full Name
RCAN1 antibody - middle region
Gene Names
RCAN1; CSP1; DSC1; RCN1; DSCR1; MCIP1; ADAPT78
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Sheep, Zebrafish
Applications
WB
Purity
Affinity Purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Daudi cells with SENP1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
SENP1, Monoclonal Antibody (Cat# AAA30500)
Full Name
SENP1 Antibody
Gene Names
SENP1; SuPr-2
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with SULT2A1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
SULT2A1, Monoclonal Antibody (Cat# AAA30489)
Full Name
SULT2A1 Antibody
Gene Names
SULT2A1; HST; ST2; STD; hSTa; DHEAS; ST2A1; ST2A3; DHEA-ST
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells with eIF4A3 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
eIF4A3, Monoclonal Antibody (Cat# AAA30480)
Full Name
eIF4A3 Antibody
Gene Names
EIF4A3; RCPS; DDX48; MUK34; NUK34; NMP265; eIF4AIII
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
HBP1, Polyclonal Antibody (Cat# AAA31326)
Full Name
Phospho-HBP1 (Ser402) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of anti-NEK2 Antibody (Center) in HL60 cell line lysates (35ug/lane). NEK2(arrow) was detected using the purified Pab.)
NEK2, Polyclonal Antibody (Cat# AAA28694)
Full Name
NEK2 Antibody (Center)
Gene Names
NEK2; NLK1; RP67; NEK2A; HsPK21; PPP1R111
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of A549 cells using anti-AGO3 antibody (AAA19290).Overlay histogram showing A549 cells stained with AAA19290 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AGO3 Antibody (AAA19290, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
AGO3, Polyclonal Antibody (Cat# AAA19290)
Full Name
Anti-AGO3 Antibody
Gene Names
AGO3; EIF2C3
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of K562 cells using anti-HNRNPH3 antibody (AAA19338).Overlay histogram showing K562 cells stained with AAA19338 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HNRNPH3 Antibody (AAA19338, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
HNRNPH3, Polyclonal Antibody (Cat# AAA19338)
Full Name
Anti-HNRNPH3 Antibody
Gene Names
HNRNPH3; 2H9; HNRPH3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of A549 cells using HDAC1 antibody. Blue: DAPI for nuclear staining.)
HDAC1, Polyclonal Antibody (Cat# AAA10632)
Full Name
HDAC1 Polyclonal Antibody
Gene Names
HDAC1; HD1; RPD3; GON-10; RPD3L1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of mouse brain using GAD2 Rabbit pAb (AAA10726) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
GAD2, Polyclonal Antibody (Cat# AAA10726)
Full Name
GAD2 Polyclonal Antibody
Gene Names
GAD2; GAD65
Applications
Western Blot, Immunofluorescence
Purity
Affinity Purification
Pricing
Application Data
(Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)
EMR1, Monoclonal Antibody (Cat# AAA26814)
Full Name
EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (MaxLight 550)
Reactivity
Mouse
Applications
FC/FACS, IHC, IP, RIA, WB
Purity
Purified by protein G affinity chromatography from tissue culture supernatant.
Pricing
IHC (Immunohistchemistry)
(At 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
CUTL1, Polyclonal Antibody (Cat# AAA31424)
Full Name
Phospho-CUTL1 (Ser1237) Antibody
Gene Names
CUX1; CDP; CUX; p75; CASP; CDP1; COY1; Clox; p100; p110; p200; CUTL1; GOLIM6; CDP/Cut; Cux/CDP; Nbla10317
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Sheep (100%), Dog (100%), Xenopus (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse brain using ACTR2 antibody at dilution of 1:100 (40x lens).)
ACTR2, Polyclonal Antibody (Cat# AAA28152)
Full Name
ACTR2 Polyclonal Antibody
Gene Names
ACTR2; ARP2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western Blot analysis of human muscle lysate (35ug protein in RIPAbuffer) using AAA24036 (0.01ug/ml). Primary incubation was 1 hour. Detected by chemiluminescence.)
COX4I1, Polyclonal Antibody (Cat# AAA24036)
Full Name
COX4I1, CT (Cytochrome C Oxidase Subunit 4 Isoform 1, Mitochondrial, Cytochrome C Oxidase Polypeptide IV, Cytochrome C Oxidase Subunit IV Isoform 1, COX IV-1, COX IV-1)
Gene Names
COX4I1; COX4; COXIV; COX4-1
Reactivity
Human
Applications
EL/EIA, WB, IHC
Purity
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Cdk6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
CDK6, Monoclonal Antibody (Cat# AAA30195)
Full Name
CDK6 Antibody
Gene Names
CDK6; PLSTIRE
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with cMet antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)
cMet, Polyclonal Antibody (Cat# AAA29965)
Full Name
cMet Antibody
Gene Names
Met; HGF; HGFR; Par4; c-Met; AI838057
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
Peptide affinity purified
Pricing
IHC (Immunohistochemistry)
(IHC image diluted at 1:1000 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
PKM, Monoclonal Antibody (Cat# AAA27048)
Full Name
PKM Monoclonal Antibody
Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein G purified
Pricing
IHC (Immunohistochemistry)
(Figure 7. IHC analysis of L1CAM using anti-L1CAM antibody (AAA19138).L1CAM was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-L1CAM Antibody (AAA19138) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
L1CAM, Polyclonal Antibody (Cat# AAA19138)
Full Name
Anti-L1CAM antibody
Gene Names
L1CAM; S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with ORP150 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ORP150, Monoclonal Antibody (Cat# AAA30512)
Full Name
ORP150 Antibody
Gene Names
HYOU1; Grp170; HSP12A; ORP150; GRP-170; ORP-150
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HUVEC cells with CD146 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
CD146, Monoclonal Antibody (Cat# AAA30472)
Full Name
CD146 Antibody
Gene Names
MCAM; CD146; MUC18
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with p16INK4A antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
p16INK4A, Monoclonal Antibody (Cat# AAA30079)
Full Name
p16INK4A Antibody
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cells using ID3 antibody.)
ID3, Polyclonal Antibody (Cat# AAA28175)
Full Name
ID3 Polyclonal Antibody
Gene Names
ID3; HEIR-1; bHLHb25
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of PC-12 cells with HMGB1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody)
HMGB1, Monoclonal Antibody (Cat# AAA29975)
Full Name
HMGB1 Antibody
Gene Names
HMGB1; HMG1; HMG3; SBP-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
WB (Western Blot)
(Western Blot analysis of Mouse, Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain cell lysate. Lane 3: Rat brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~30 kDa (dimer).)
Alpha Synuclein, Monoclonal Antibody (Cat# AAA17810)
Full Name
Alpha Synuclein Antibody, Clone 3C11
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human; Mouse; Rat
Applications
WB, DB, ICC, IF, EIA
Purity
Protein G Purified
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of Raji cells using anti-CD38 antibody (AAA19217).Overlay histogram showing Raji cells stained with AAA19217 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD38 Antibody (AAA19217, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CD38, Polyclonal Antibody (Cat# AAA19217)
Full Name
Anti-CD38 Antibody
Gene Names
CD38; T10; ADPRC 1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry
Purity
Immunogen affinity purified.
Pricing