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Gene Name

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Host

Application

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10000 results for "Protein" - showing 9450-9500


product-image-AAA15915_WB.jpg WB (Western Blot) (Western BlotPositive WB detected in: Rosseta bacteria lysate at 80ug, 40ug, 20ug, 10ugAll lanes: rpsB antibody at 2.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionpredicted band size: 27 kDaobserved band size: 27 kDa)

30S ribosomal protein S2, Polyclonal Antibody (Cat# AAA15915)

Full Name
Rabbit anti-Escherichia coli 30S ribosomal protein S2 polyclonal Antibody
Gene Names
rpsB; ECK0168; JW0164
Reactivity
Escherichia coli
Applications
Western Blot
Purity
>95%, Protein G purified
Pricing

anti-Cotton Rat IgG h+l, Polyclonal Antibody (Cat# AAA14444)

Full Name
HRP Conjugated Chicken anti-Cotton Rat IgG h+l
Applications
Immunohistochemistry
Pricing

C3, Antibody (Cat# AAA14446)

Full Name
FITC Conjugated Goat anti-Mouse C3
Applications
Immunofluorescence
Pricing

H3 (A/Shangdong/9/93) (H3N2), Monoclonal Antibody (Cat# AAA13686)

Full Name
Anti-H3 (A/Shangdong/9/93) (H3N2), clone 227
Applications
Western Blot, Sandwich ELISA
Purity
Chromatography on protein G Sepharose
Pricing
product-image-AAA27913_IHC2.jpg IHC (Immunohistochemistry)

DEPDC5, Antibody (Cat# AAA27913)

Full Name
DEPDC5 Antibody
Gene Names
DEPDC5; DEP.5; FFEVF; FFEVF1
Reactivity
Reacts with: Human
Predicted: Rat, Mouse
Not yet tested in other species.
Applications
Western Blot, Immunohistochemistry
Pricing
product-image-AAA13881_APP2.jpg Application Data (Anti-TurboRFP Ab at 1/2,500 dilution using HEK293 transfected cell lysates at 50 ug per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;)

TurboRFP, Polyclonal Antibody (Cat# AAA13881)

Full Name
anti-TurboRFP
Reactivity
Transfected cells proteins
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Immunoelectron Microscopy
Purity
This antibody is epitope-affinity purified from goat antiserum.
Pricing

Xin actin-binding repeat-containing protein 1, ELISA Kit (Cat# AAA13274)

Full Name
Human Xin actin-binding repeat-containing protein 1 (XIRP1) ELISA Kit
Reactivity
Human
Pricing
product-image-AAA28789_APP6.jpg Application Data (Overlay histogram showing U-2OS cells stained with AP10510c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated n 2% bovine serum albumin to block non-specificprotein-protein interactions followed by the antibody (AP10510c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used wasGoat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events wasperformed)

CF150, Polyclonal Antibody (Cat# AAA28789)

Full Name
CF150 Antibody (Center)
Gene Names
MB21D1; cGAS; h-cGAS; C6orf150
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA11769_SDS_PAGE.jpg SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

Midkine, Recombinant Protein (Cat# AAA11769)

Full Name
Midkine, 21-143aa, Human, His tag, E Coli
Gene Names
MDK; MK; ARAP; NEGF2
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
product-image-AAA26951_IHC2.jpg IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using AAA26951 at dilution 1:100)

MMP19, Polyclonal Antibody (Cat# AAA26951)

Full Name
MMP19 Antibody
Gene Names
MMP19; CODA; MMP18; RASI-1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Pricing

Prothrombin, Native Protein (Cat# AAA14354)

Full Name
Prothrombin protein (Bovine)
Purity
> 98% by SDS-PAGE
Pricing

Alpha-1-Antitrypsin (SERPINA1), ELISA Kit (Cat# AAA23136)

Full Name
Canine Alpha-1-Antitrypsin (SERPINA1) ELISA Kit
Reactivity
Canine
Pricing
product-image-AAA11749_SDS_PAGE.jpg SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

HMGB1, Recombinant Protein (Cat# AAA11749)

Full Name
HMGB1, 1-215aa, Human, His tag, Insect cell
Gene Names
HMGB1; HMG1; HMG3; HMG-1; SBP-1
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing

COVID 19 Spike RBD IgM Humanized Coronavirus, Monoclonal Antibody (Cat# AAA13547)

Full Name
Anti-SARS-CoV-2 Spike RBD IgM, Humanized antibody (Positive Control)
Reactivity
Viral
Applications
Lateral Flow
Purity
Purification: Protein A purified
Purity: >95% by HPLC & SDS-PAGE
Pricing
product-image-AAA17978_WB.jpg WB (Western Blot) (Western blot analysis of extracts from LOVO cells and HepG2 cells, using CA6 antibody.)

CA6, Antibody (Cat# AAA17978)

Full Name
CA6 Antibody
Gene Names
CA6; CA-VI; GUSTIN
Reactivity
Human (Identities = 100%, Positives = 100%)
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing

SEX HORMONE BINDING GLOBULIN, Native Protein (Cat# AAA12299)

Full Name
PURIFIED HUMAN SEX HORMONE BINDING GLOBULIN
Gene Names
SHBG; ABP; SBP; TEBG
Applications
Immunoassay
Purity
>90% by SDS PAGE
Pricing
product-image-AAA13867_IEM8.jpg IEM (Immunogold Electron Microscopy) (Immunogold labeling of RPE, in vivo injected with mCherry expressing vector)

mCherry, Polyclonal Antibody (Cat# AAA13867)

Full Name
mCherry Polyclonal Antibody
Reactivity
Transfected cells proteins
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, Immunohistochemistry, Immunoelectron Microscopy
Pricing
product-image-AAA27928_WB.jpg WB (Western Blot) (Western blot analysis of extracts of rat thymus, using T2R38 antibody (AAA27928) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.)

T2R38, Polyclonal Antibody (Cat# AAA27928)

Full Name
Taste receptor type 2 member 38 (T2R38) (PTC bitter taste receptor) (Taste receptor type 2 member 61)
Gene Names
TAS2R38; PTC; T2R38; T2R61
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing

CA 15-3, Native Protein (Cat# AAA14341)

Full Name
CA 15-3 protein
Purity
Purity Ratio: 5,057.95 U/ml/OD
Pricing

CA 125, Native Protein (Cat# AAA14342)

Full Name
CA 125 Ag protein (preservative free)
Reactivity
CA 19-9:<1.0%, CA 72-4<1.0%, CA15-3:<0.1%
Applications
Control
Purity
> 95% pure
Pricing

CA 125, Monoclonal Antibody (Cat# AAA14302)

Full Name
CA 125 antibody
Reactivity
High affinity to Epitope group A
Applications
Immunoassay, Immunoblot
Pricing
product-image-AAA25088_WB7.jpg WB (Western Blot) (EIF4G1 monoclonal antibody. Western Blot analysis of EIF4G1 expression in NIH/3T3.)

EIF4G1, Monoclonal Antibody (Cat# AAA25088)

Full Name
EIF4G1 (Eukaryotic Translation Initiation Factor 4 gamma 1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220, EIF4G1, EIF4F, EIF4G, EIF4GI) (FITC)
Gene Names
EIF4G1; P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing

HLA-C, Antibody (Cat# AAA20035)

Full Name
Anti-HLA-C Purified
Gene Names
HLA-C; HLC-C; D6S204; PSORS1; HLA-JY3
Reactivity
Non-human primates, Human
Applications
Immunoprecipitation, Flow Cytometry
Purity
Purified by protein-A affinity chromatography.
Pricing

HVEM-Fc, Active Protein (Cat# AAA14394)

Full Name
HVEM-Fc protein
Purity
>= 98% by SDS-PAGE and HPLC
Pricing
product-image-AAA29757_WB.jpg WB (Western Blot) (Western blot analysis Rpt6 (Phospho-Ser120) using 293 whole cell lysates)

Rpt6, Polyclonal Antibody (Cat# AAA29757)

Full Name
Rpt6 (Phospho-Ser120) Antibody
Gene Names
PSMC5; S8; p45; SUG1; SUG-1; TBP10; TRIP1; p45/SUG
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Pricing
product-image-AAA11804_SDS_PAGE.jpg SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)

SEPSECS, Recombinant Protein (Cat# AAA11804)

Full Name
SEPSECS, 1-501aa, Human, His tag, E Coli
Gene Names
SEPSECS; LP; SLA; PCH2D; SLA/LP
Applications
SDS-PAGE
Purity
> 90% by SDS-PAGE
Pricing
product-image-AAA26960_IF4.jpg IF (Immunofluorescence) (Immunofluorescence staining of MCF-7 cells with AAA26960 at 1:100, counter- stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

IFNLR1, Polyclonal Antibody (Cat# AAA26960)

Full Name
IFNLR1 Antibody
Gene Names
IFNLR1; IFNLR; LICR2; IL28RA; CRF2/12; IL-28R1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antigen Affinity Purified
Pricing
product-image-AAA14697_IF2.jpg IF (Immunofluorescence) (Immunofluorescence (IFC) analysis of PC3 cells using AAA14697 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)

Ribonuclease inhibitor 1, Monoclonal Antibody (Cat# AAA14697)

Full Name
Ribonuclease inhibitor 1 (RNH1)
Gene Names
RNH1; RAI; RNH; MGC4569; MGC18200; MGC54054
Applications
Immunofluorescence, Western Blot
Purity
Affinity Purified
Purified by Protein G affinity chromatography.
Pricing
product-image-AAA13872_IHC8.jpg IHC (Immunohistochemistry) (IHC of human adipose tissue anti-CANX antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CANX Ab at 1:750/DAB detection.)

CANX, Polyclonal Antibody (Cat# AAA13872)

Full Name
Calnexin Polyclonal Antibody
Gene Names
canx; zgc:63524; wu:fe06b12
Reactivity
Human, Rat, Mouse, Monkey, Canine proteins
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, Immunohistochemistry
Pricing
product-image-AAA29648_SDS_PAGE4.jpg SDS-PAGE (Sample (30 ug of whole cell lysate)A: HelaB: JurKat5% SDS PAGEPrimary antibody diluted at 1: 1000)

LTBP-4, Polyclonal Antibody (Cat# AAA29648)

Full Name
LTBP-4 antibody
Gene Names
LTBP4; ARCL1C; LTBP-4; LTBP4L; LTBP4S
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
Antigen-affinity purification
Pricing
product-image-AAA12041_APP6.gif Application Data (Staining of mouse peripheral blood platelets with Hamster anti Mouse CD61:FITC)

CD61, Monoclonal Antibody (Cat# AAA12041)

Full Name
HAMSTER ANTI MOUSE CD61:RPE
Gene Names
Itgb3; CD61; GP3A; INGRB3
Applications
Flow Cytometry
Pricing
product-image-AAA23385_WB9.jpg WB (Western Blot) (25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.)

RIPK3, Polyclonal Antibody (Cat# AAA23385)

Full Name
RIPK3 antibody - N-terminal region
Gene Names
RIPK3; RIP3
Reactivity
Tested Species Reactivty: Human
Predicted Species Reactivity: Cow, Dog, Horse, Human, Mouse, Pig, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Protein A purified
Pricing
product-image-AAA12241_APP.gif Application Data (Human C-Reactive Protein detected using Goat anti Human C-Reactive Protein (AAA12241))

C-REACTIVE PROTEIN, Polyclonal Antibody (Cat# AAA12241)

Full Name
GOAT ANTI HUMAN C-REACTIVE PROTEIN
Gene Names
CRP; PTX1
Reactivity
Human
Applications
Immunodiffusion
Purity
Purified IgG by affinity chromatography.
Pricing
product-image-AAA27037_APP3.jpg Colloidal Gold Immunochromatography Assay (In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (AAA27037) is clean, the detection limit can be as low as 125ng/ml (8.75ng/0.07ml), and the sensitivity is very good.)

COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Recombinant Antibody (Cat# AAA27037)

Full Name
Human Novel Coronavirus Nucleoprotein (N) (1-419aa) Antibody
Reactivity
Human Novel Coronavirus (SARS-CoV-2/2019-nCoV)
Applications
Western Blot, Gold Immunochromatography Assay
Purity
Affinity-chromatography
Pricing
product-image-AAA27038_APP3.jpg Application Data (ELISA: Immobilize various types of SARS proteins at concentration of 2g/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100g/ml, 10g/ml and 1g/ml. It shows the SARS-CoV-2-N Antibody (AAA27038) is specific for SARS-CoV-2-N protein, without any cross- reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1.)

COVID 19 Nucleocapsid (NP) Coronavirus, Monoclonal Recombinant Antibody (Cat# AAA27038)

Full Name
Human Novel Coronavirus Nucleoprotein (N) (1-419aa) Antibody
Reactivity
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Applications
Colloidal Gold Immunochromatographic Assay
Purity
Affinity-chromatography
Pricing
product-image-AAA30413_FCM6.jpg FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with Growth hormone receptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

Growth Hormone, Monoclonal Antibody (Cat# AAA30413)

Full Name
Growth Hormone Receptor Antibody
Gene Names
GHR; GHBP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing

Alanine Aminotransferase (ALT), Polyclonal Antibody (Cat# AAA20296)

Full Name
Polyclonal Antibody to Alanine Aminotransferase (ALT)
Gene Names
Gpt; ALT; ALT1; Gpt1; Gpt-1; 1300007J06Rik; 2310022B03Rik
Reactivity
Mouse
Applications
Westen Blot, Immunohistochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
product-image-AAA30018_FCM10.jpg FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with VEGF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

VEGF, Monoclonal Antibody (Cat# AAA30018)

Full Name
VEGF Antibody
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
product-image-AAA13804_FCM10.jpg FCM (Flow Cytometry) (Flow Cytometric Analysis of PFA-fixed Raji cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).)

Human Nuclear Antigen, Monoclonal Antibody (Cat# AAA13804)

Full Name
Human Nuclear Antigen
Reactivity
Human and Non-human primates. Does not react with mouse, rat, and chicken. Others not known.
Applications
Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry
Pricing

CORONAVIRUS, Monoclonal Antibody (Cat# AAA11955)

Full Name
MOUSE ANTI FELINE CORONAVIRUS
Reactivity
Feline
Applications
Flow Cytometry, Immunohistochemistry, Immunohistochemistry, Western Blot, Immunofluorescence, Immunoprecipitation
Pricing
product-image-AAA11520_IHC2.jpg IHC (Immunohistochemistry) (Anti-5HT1A Receptor antibody, AAA11520, IHC(P)IHC(P): Rat Brain Tissue)

5HT1A Receptor, Polyclonal Antibody (Cat# AAA11520)

Full Name
Anti-5HT1A Receptor antibody
Gene Names
HTR1A; G-21; 5HT1a; PFMCD; 5-HT1A; 5-HT-1A; ADRBRL1; ADRB2RL1
Reactivity
Reacts with: Human, Rat
Predicted to work with: Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
product-image-AAA30941_IHC2.jpg IHC (Immunohistochemistry) (AAA30941 at 1/100 staining Human lung cancer by IHC-P. Thesample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

AC-FKHR, Polyclonal Antibody (Cat# AAA30941)

Full Name
AC-FKHR(K294) Antibody
Gene Names
FOXO1; FKH1; FKHR; FOXO1A
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry
Purity
Peptide affinity purification
Pricing
product-image-AAA11510_IHC2.jpg IHC (Immunohistochemistry) (Anti-5HT2A Receptor antibody, AAA11510, IHC(P)IHC(P): Rat Brain Tissue)

5HT2A Receptor, Polyclonal Antibody (Cat# AAA11510)

Full Name
Anti-5HT2A Receptor antibody
Gene Names
HTR2A; HTR2; 5-HT2A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
product-image-AAA27786_IF3.jpg IF (Immunofluorescence) (Immunofluorescent analysis of Cytokeratin 20 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

Cytokeratin 20, Polyclonal Antibody (Cat# AAA27786)

Full Name
Anti-Cytokeratin 20 Antibody
Gene Names
KRT20; K20; CD20; CK20; CK-20; KRT21
Reactivity
Human, Mouse, Rat, Bovine, Pig
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
product-image-AAA28802_WB.jpg WB (Western Blot) (Anti-SLC6A19 Antibody (C-Term) at 1:2000 dilution + 293 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 71 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

SLC6A19, Polyclonal Antibody (Cat# AAA28802)

Full Name
SLC6A19 Antibody (C-Term)
Gene Names
SLC6A19; HND; B0AT1
Reactivity
Human
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
product-image-AAA11646_IHC8.jpg IHC (Immunohistochemistry) (Anti- TGM2 Picoband antibody, AAA11646, IHC(F)IHC(F): Human Placenta Tissue)

TGM2, Polyclonal Antibody (Cat# AAA11646)

Full Name
Anti-TGM2 Antibody
Gene Names
TGM2; TGC; TG(C)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
product-image-AAA30955_IF6.jpg IF (Immunofluorescence) (AAA30955 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

JAK2, Polyclonal Antibody (Cat# AAA30955)

Full Name
Phospho-JAK2 (Tyr931) Antibody
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA11517_WB.jpg WB (Western Blot) (Figure. Western blot analysis of IFN gamma using anti- IFN gamma antibody (AAA11517).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane: Recombinant Human IFN gamma Protein 0.5ngAfter Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- IFN gamma antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IFN gamma at approximately 17KD. The expected band size for IFN gamma is at 17KD.)

Interferon gamma, Polyclonal Antibody (Cat# AAA11517)

Full Name
Anti-human Interferon gamma antibody
Gene Names
IFNG; IFG; IFI
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Neutralization Assay, Immunoprecipitation
Purity
Immunogen affinity purified.
Pricing
product-image-AAA31211_IF6.jpg IF (Immunofluorescence) (AAA31211 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31211 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

SEPT4, Polyclonal Antibody (Cat# AAA31211)

Full Name
SEPT4 Antibody
Gene Names
SEPTIN4; H5; ARTS; MART; SEP4; CE5B3; SEPT4; PNUTL2; hucep-7; BRADEION; C17orf47; hCDCREL-2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA27681_IP7.png IP (Immunoprecipitation) (Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 5A9. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4 degree C and next incubated with 250ug of MEF lysate for 2 hours at 4 degree C.)

VPS35, Monoclonal Antibody (Cat# AAA27681)

Full Name
VPS35 Antibody, Clone 5A9: RPE
Gene Names
VPS35; MEM3; PARK17
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein G Purified
Pricing

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