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998 results for "Rabbit Anti Human IgG H L " - showing 900-950
IF (Immunofluorescence)
(AAA30936 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
NF kappaB p65, Polyclonal Antibody (Cat# AAA30936)
Full Name
Phospho-NF kappaB p65 (Ser536) Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of FOXO4 phosphorylation expression in EGF treated HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
FOXO4, Polyclonal Antibody (Cat# AAA31061)
Full Name
Phospho-FOXO4 (Thr451) Antibody
Gene Names
FOXO4; AFX; AFX1; MLLT7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Ezrin phosphorylation expression in Na3VO4 treated K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Ezrin, Polyclonal Antibody (Cat# AAA31010)
Full Name
Phospho-Ezrin (Tyr478) Antibody
Gene Names
EZR; CVL; CVIL; VIL2; HEL-S-105
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31054 at 1/200 staining Mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
JNK1/2/3, Polyclonal Antibody (Cat# AAA31054)
Full Name
Phospho-JNK1/2/3 (Thr183+Tyr185) Antibody
Gene Names
MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Dnmt1, Polyclonal Antibody (Cat# AAA31301)
Full Name
Phospho-Dnmt1 (Ser714) Antibody
Gene Names
DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
Peptide affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2OS cells using RAB7A antibody (A12784) at dilution of 1:100. Blue: DAPI for nuclear staining.)
RAB7A, Polyclonal Antibody (Cat# AAA28256)
Full Name
RAB7A Polyclonal Antibody
Gene Names
RAB7A; RAB7; PRO2706
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of lysate from mouse heart tissue lysate, using MYBPC3 Antibody (N-term). AAA28794 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
MYBPC3, Polyclonal Antibody (Cat# AAA28794)
Full Name
MYBPC3 Antibody (N-term)
Gene Names
MYBPC3; FHC; CMH4; CMD1MM; LVNC10; MYBP-C
Reactivity
Human, Mouse, Rat
(Predicted Reactivity: Rat)
(Predicted Reactivity: Rat)
Applications
Immunohistochemistry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH-3T3 cells using mTOR Rabbit pAb (AAA28241) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)
MTOR, Polyclonal Antibody (Cat# AAA28241)
Full Name
MTOR Polyclonal Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Western blot analysis of lysates from 293T cell line human brain and mouse brain tissue lysate(from left to right), using STRADA Antibody (C-term). AAA28673 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
STRADA, Polyclonal Antibody (Cat# AAA28673)
Full Name
STRADA Antibody (C-term)
Gene Names
STRADA; LYK5; PMSE; Stlk; STRAD; NY-BR-96
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Applications
Western Blot, Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(AAA28674 staining GLUL in Human liver tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
GLUL, Polyclonal Antibody (Cat# AAA28674)
Full Name
GLUL Antibody (N-term)
Gene Names
GLUL; GS; GLNS; PIG43; PIG59
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Chicken, Monkey, Pig)
Applications
Western Blot, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(staining A549 cells cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor® 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Cox2, Polyclonal Antibody (Cat# AAA31143)
Full Name
Cox2 Antibody
Gene Names
PTGS2; COX2; COX-2; PHS-2; PGG/HS; PGHS-2; hCox-2; GRIPGHS
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-HDAC3 (Ser424) Antibody expression in mouse lung and mouse brain tissues lysates.The lane on the right is treated with the antigen-specific peptide.)
HDAC3, Polyclonal Antibody (Cat# AAA30951)
Full Name
Phospho-HDAC3 (Ser424) Antibody
Gene Names
HDAC3; HD3; RPD3; RPD3-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31053 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
GATA4, Polyclonal Antibody (Cat# AAA31053)
Full Name
Phospho-GATA4 (Ser262) Antibody
Gene Names
GATA4; TOF; ASD2; VSD1; TACHD
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30948 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Chk1, Polyclonal Antibody (Cat# AAA30948)
Full Name
Phospho-Chk1 (Ser317) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of XIAP phosphorylation expression in Anisomycin treated HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
XIAP, Polyclonal Antibody (Cat# AAA31060)
Full Name
Phospho-XIAP (Ser87) Antibody
Gene Names
XIAP; API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30995 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
BCL-2, Polyclonal Antibody (Cat# AAA30995)
Full Name
Phospho-BCL-2 (Ser70) Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31265 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31265) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
PPP2R5C, Polyclonal Antibody (Cat# AAA31265)
Full Name
PPP2R5C Antibody
Gene Names
PPP2R5C; B56G; PR61G; B56gamma
Reactivity
Human, Rat
Predicted Reactivity: Rabbit(88%)
Predicted Reactivity: Rabbit(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(AAA30934 at 1/100 staining human TB tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
I kappaB alpha, Polyclonal Antibody (Cat# AAA30934)
Full Name
Phospho-I kappaB alpha (Ser32/Ser36) Antibody
Gene Names
NFKBIA; IKBA; MAD-3; NFKBI
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(RSPO2 Antibody (C-term) (AAA28708) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
RSPO2, Polyclonal Antibody (Cat# AAA28708)
Full Name
RSPO2 Antibody (C-term)
Gene Names
RSPO2; CRISTIN2
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Westen Blot, Immunohistochemistry, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western blot analysis of LoVo cell lysate, using PIBF1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
PIBF1, Polyclonal Antibody (Cat# AAA31130)
Full Name
PIBF1 Antibody
Gene Names
PIBF1; PIBF; CEP90; JBTS33; C13orf24
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
WB (Western Blot)
(Western blot analysis of Chk2 phosphorylation expression in Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Chk2, Polyclonal Antibody (Cat# AAA30957)
Full Name
Phospho-Chk2 (Thr387) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of p53 phosphorylation expression in HU treated HeLa whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
p53, Polyclonal Antibody (Cat# AAA30977)
Full Name
Phospho-p53 (Ser15) Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PAR4, Polyclonal Antibody (Cat# AAA31375)
Full Name
Phospho-PAR4 (Thr163) Antibody
Gene Names
PAWR; PAR4; Par-4
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (92%), Rabbit (100%), Chicken (100%), Xenopus (92%)
Predicted Reactivity: Pig (100%), Zebrafish (92%), Rabbit (100%), Chicken (100%), Xenopus (92%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Validation Data Western blot analysis of extracts of various cell lines, using GAA antibody (AAA28197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 60s)
GAA, Polyclonal Antibody (Cat# AAA28197)
Full Name
GAA Polyclonal Antibody
Gene Names
GAA; LYAG
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation was performed with cross-linked chromatin from 293T, using Acetyl-Histone H3-K27 Rabbit pAb antibody (AAA28163) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram constructed the ratios of the ratio of the immunoprecipitated DNA versus the input.)
H3K27AC, Antibody (Cat# AAA28163)
Full Name
Histone H3K27AC Polyclonal Antibody
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts of rat thymus, using T2R38 antibody (AAA27928) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.)
T2R38, Polyclonal Antibody (Cat# AAA27928)
Full Name
Taste receptor type 2 member 38 (T2R38) (PTC bitter taste receptor) (Taste receptor type 2 member 61)
Gene Names
TAS2R38; PTC; T2R38; T2R61
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohistchemistry)
(Figure 9 Immunohistochemistry Validation of IRF7 in Human Liver Tissue Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IRF7 antibody (8991) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IRF7, Polyclonal Antibody (Cat# AAA11032)
Full Name
IRF7 Antibody
Gene Names
IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
IRF7 Antibody is Protein A purified.
Pricing
IHC (Immunohistochemistry)
(AAA30939 at 1/100 staining rat appendiceal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Chk1, Polyclonal Antibody (Cat# AAA30939)
Full Name
Phospho-Chk1 (Ser280) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30955 staining A549 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
JAK2, Polyclonal Antibody (Cat# AAA30955)
Full Name
Phospho-JAK2 (Tyr931) Antibody
Gene Names
JAK2; JTK10; THCYT3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(Overlay histogram showing U-2OS cells stained with AP10510c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated n 2% bovine serum albumin to block non-specificprotein-protein interactions followed by the antibody (AP10510c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used wasGoat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events wasperformed)
CF150, Polyclonal Antibody (Cat# AAA28789)
Full Name
CF150 Antibody (Center)
Gene Names
MB21D1; cGAS; h-cGAS; C6orf150
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Anti-SLC6A19 Antibody (C-Term) at 1:2000 dilution + 293 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 71 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
SLC6A19, Polyclonal Antibody (Cat# AAA28802)
Full Name
SLC6A19 Antibody (C-Term)
Gene Names
SLC6A19; HND; B0AT1
Reactivity
Human
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(AAA31046 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
STAT6, Polyclonal Antibody (Cat# AAA31046)
Full Name
Phospho-STAT6 (Thr645) Antibody
Gene Names
STAT6; STAT6B; STAT6C; D12S1644; IL-4-STAT
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31015 at 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
CREB, Polyclonal Antibody (Cat# AAA31015)
Full Name
Phospho-CREB (Ser142) Antibody
Gene Names
CREB1; CREB; CREB-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31211 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31211 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
SEPT4, Polyclonal Antibody (Cat# AAA31211)
Full Name
SEPT4 Antibody
Gene Names
SEPTIN4; H5; ARTS; MART; SEP4; CE5B3; SEPT4; PNUTL2; hucep-7; BRADEION; C17orf47; hCDCREL-2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing U87 cells stained with AAA28063 (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
CD9, Monoclonal Antibody (Cat# AAA28063)
Full Name
CD9 Monoclonal Antibody
Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
>95%, Protein G purified
Pricing
IF (Immunofluorescence)
(AAA30966 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Myc, Polyclonal Antibody (Cat# AAA30966)
Full Name
Phospho-Myc (Thr58) Antibody
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30967 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Lamin A/C, Polyclonal Antibody (Cat# AAA30967)
Full Name
Phospho-Lamin A/C (Ser392) Antibody
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30924 staining MCF-7 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Ki67, Polyclonal Antibody (Cat# AAA30924)
Full Name
Ki67 Antibody
Gene Names
MKI67; KIA; MIB-; MIB-1; PPP1R105
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientifiv).
Pricing
IF (Immunofluorescence)
(AAA30990 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Lyn, Polyclonal Antibody (Cat# AAA30990)
Full Name
Phospho-Lyn (Tyr508) Antibody
Gene Names
LYN; JTK8; p53Lyn; p56Lyn
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immmunohistochemistry)
(PLA2G7 Antibody (Center) (Cat. #AAA28698)IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PLA2G7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
PLA2G7, Polyclonal Antibody (Cat# AAA28698)
Full Name
PLA2G7 Antibody (Center)
Gene Names
PLA2G7; PAFAD; PAFAH; LP-PLA2; LDL-PLA2
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H3, Polyclonal Antibody (Cat# AAA31353)
Full Name
Acetyl-Histone H3 (Lys122) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%)
Predicted Reactivity: Bovine (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2 OS cells using CLUH Polyclonal Antibody (AAA28214) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
CLUH, Polyclonal Antibody (Cat# AAA28214)
Full Name
CLUH Polyclonal Antibody
Gene Names
CLUH; CLU1; KIAA0664
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Affinity purification
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300 ?g extracts of HeLa cells using 3 ?g DBI antibody (AAA28262). Western blot was performed from the immunoprecipitate using DBI antibody (AAA28262) at a dilution of 1:1000.)
DBI, Polyclonal Antibody (Cat# AAA28262)
Full Name
DBI Polyclonal Antibody
Gene Names
DBI; EP; ACBP; ACBD1; CCK-RP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(AAA30991 at 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary Ab.)
SP1, Polyclonal Antibody (Cat# AAA30991)
Full Name
Phospho-SP1 (Thr453) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30921 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CCR5, Polyclonal Antibody (Cat# AAA30921)
Full Name
Phospho-CCR5(Ser349) Antibody
Gene Names
CCR5; CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
IHC (Immunohistchemistry)
(AAA30994 at 1/200 staining Mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IGF1R, Polyclonal Antibody (Cat# AAA30994)
Full Name
Phospho-IGF1R (Tyr1161) Antibody
Gene Names
IGF1R; IGFR; CD221; IGFIR; JTK13
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30959 at 1/100 staining rat testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Chk2, Polyclonal Antibody (Cat# AAA30959)
Full Name
Phospho-Chk2 (Ser516) Antibody
Gene Names
CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-EGFR (Ser1071) expression in various lysates)
EGFR, Polyclonal Antibody (Cat# AAA30964)
Full Name
Phospho-EGFR (Ser1071) Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of MMP12 Antibody (C-term) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
MMP12, Polyclonal Antibody (Cat# AAA28713)
Full Name
MMP12 Antibody (C-term)
Gene Names
MMP12; ME; HME; MME; MMP-12
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(AAA30935 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
GR, Polyclonal Antibody (Cat# AAA30935)
Full Name
Phospho-GR (Ser211) Antibody
Gene Names
NR3C1; GR; GCR; GRL; GCCR; GCRST
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing