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Type
Reactivity
Gene Name
Isotype
Host
Application
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951 results for "Synthetic Peptide" - showing 800-850
IHC (Immunohistchemistry)
(Fig.6. Immunohistochemical analysis of paraffin-embedded rat testis tissue. 1, PCNA Monoclonal Antibod y(1D7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
PCNA, Monoclonal Antibody (Cat# AAA31541)
Full Name
Anti-PCNA Mouse Monoclonal Antibody (1D7)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Pricing
WB (Western Blot)
(Anti-CCR5 antibody, AAA11522, IHC(P)All lanes: Anti CCR5 (AAA11522) at 0.5ug/mlWB: Mouse Liver Tissue Lysate at 50ugPredicted bind size: 40KDObserved bind size: 40KD)
CCR5, Polyclonal Antibody (Cat# AAA11522)
Full Name
Anti-CCR5 antibody
Gene Names
CCR5; CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
Reactivity
Reacts with: Human, Mouse
Predicted to work with: Rat
Predicted to work with: Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of BAF250B staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
BAF250B, Polyclonal Antibody (Cat# AAA27801)
Full Name
Anti-BAF250B Antibody
Gene Names
ARID1B; OSA2; 6A3-5; DAN15; MRD12; P250R; BRIGHT; BAF250B; ELD/OSA1
Reactivity
Human, Mouse, Rat, Porcine
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
FCM (Flow Cytometry)
(CB2 Antibody (C-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
CB2, Polyclonal Antibody (Cat# AAA28672)
Full Name
CB2 Antibody (C-term)
Gene Names
CNR2; CB2; CX5; CB-2
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(Anti-NR6A1 / GCNF antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
NR6A1 / GCNF, Polyclonal Antibody (Cat# AAA12361)
Full Name
Rabbit Polyclonal to Human NR6A1 / GCNF
Gene Names
NR6A1; RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Applications
Immunohistochemistry
Purity
Immunoaffinity Purified
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded Human skin tissue with Phospho-MLKL (Ser358) Monoclonal Antibody at dilution of 1:200)
MLKL, Monoclonal Antibody (Cat# AAA22113)
Full Name
Phospho-MLKL (Ser358) Monoclonal Antibody
Gene Names
MLKL; hMLKL
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A purification
Pricing
WB (Western Blot)
(Western blot analysis on human serum using anti- Complement C3 mouse monoclonal antibody.)
Complement C3, Antibody (Cat# AAA17956)
Full Name
Complement C3 (betachain) Antibody
Gene Names
C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
Reactivity
Human
Applications
Western Blot
Pricing
IHC (Immunohistochemistry)
(The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using AAA29763 (ISM1 Antibody) at dilution 1/60.(Original magnification: 200))
ISM1, Polyclonal Antibody (Cat# AAA29763)
Full Name
ISM1 Antibody
Gene Names
ISM1; ISM; Isthmin; C20orf82; bA149I18.1; dJ1077I2.1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Pricing
Application Data
IRAK4 (pT345), Monoclonal Antibody (Cat# AAA14096)
Full Name
Mouse anti Phospho-IRAK-4 (pThr345)
Gene Names
IRAK4; IPD1; REN64; NY-REN-64
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The Mouse IgG is purified by Isotyping-specific Affinity Purification.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded mouse kidney using SNRNP70 antibody (AAA28160) at dilution of 1:100 (40x lens).)
SNRNP70, Polyclonal Antibody (Cat# AAA28160)
Full Name
SNRNP70 Polyclonal Antibody
Gene Names
SNRNP70; RPU1; Snp1; U1AP; U170K; U1RNP; RNPU1Z; SNRP70; U1-70K
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of DNA Polymerase zeta staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
DNA Polymerase zeta, Polyclonal Antibody (Cat# AAA27811)
Full Name
Anti-DNA Polymerase zeta Antibody
Gene Names
REV3L; POLZ; REV3
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Pig
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
CIAS1/NALP3, Polyclonal Antibody (Cat# AAA11523)
Full Name
Anti-CIAS1/NALP3 antibody
Gene Names
NLRP3; AII; AVP; FCU; MWS; FCAS; CIAS1; NALP3; C1orf7; CLR1.1; PYPAF1; AGTAVPRL
Reactivity
Human, Rat; Predicted: Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry
Purity
Immunogen affinity purified
Pricing
WB (Western Blot)
(WB Suggested Anti-ACTN1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: 293T cell lysate)
ACTN1, Polyclonal Antibody (Cat# AAA23579)
Full Name
ACTN1 antibody - N-terminal region
Gene Names
ACTN1; BDPLT15
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Pricing
WB (Western Blot)
(Western blot analysis of BMP2 expression in HepG2 (A); HeLa (B) whole cell lysates.)
BMP2, Polyclonal Antibody (Cat# AAA12380)
Full Name
Anti-BMP2 Antibody (C-Terminus) IHC-plus
Gene Names
BMP2; BDA2; BMP2A
Reactivity
Chicken, Zebrafish, Pig, Rabbit, Mouse, Sheep, Bovine, Rat, Human
Applications
Immunohistochemistry, Western Blot
Purity
Immunoaffinity purified
Pricing
IHC (Immunohistochemistry)
(Anti-SLC7A5 antibody IHC of human placenta, terminal villi. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)
SLC7A5 / CD98, Polyclonal Antibody (Cat# AAA12328)
Full Name
SLC7A5 / CD98 Light Chain
Gene Names
SLC7A5; E16; CD98; LAT1; 4F2LC; MPE16; hLAT1; D16S469E
Reactivity
Human, Monkey, Dog.
Predicted: Bat, Rabbit
Predicted: Bat, Rabbit
Applications
Immunohistochemistry
Purity
Immunoaffinity Purified
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry: Human breast carcinoma tissue (FFPE) stained with Anti-STAT5 (pY694) (Cat# AAA14089) antibody at 1:100 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
STAT5 (pY694), Antibody (Cat# AAA14089)
Full Name
Rabbit anti Phospho- STAT5 (pTyr 694)
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Pricing
WB (Western Blot)
(Anti- ERV31 antibody, Western blotting)
ERV31, Polyclonal Antibody (Cat# AAA11665)
Full Name
Anti-ERV31 Antibody
Gene Names
ERV3-1; ERV3; ERVR; envR; ERV-R; HERVR; HERV-R
Reactivity
Human
Applications
Western Blot
Purity
Immunogen Affinity Purified
Pricing
Application Data
LRP6 (phospho), Antibody (Cat# AAA14084)
Full Name
Rabbit anti LRP6 Phospho-specific antibody
Gene Names
LRP6; ADCAD2; FLJ90062; FLJ90421
Reactivity
Human, Rat, Mouse, Bovine, Canine
Applications
Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by Site-specific Epitope Affinity Purification.
Pricing
WB (Western Blot)
(Western Blot of Rat brain using AAA14727 (1ug/mL). The signal was developed with IRDyeTM800 conjugated giat anti-rabbit IgG)
Tau, Polyclonal Antibody (Cat# AAA14727)
Full Name
Tau, phosphorylated (Thr217) (Tau Protein, Microtubule-associated Protein, MAP)
Gene Names
tau; BcDNA:RE16764; CG12881; CG31057; CG5606; DmelCG31057; dtau
Reactivity
Human
Applications
Western Blot
Purity
Purified by Affinity chromatography.
Pricing
WB (Western Blot)
(Anti-EMP1 Antibody (N-term) at 1:500 dilution + Human skeletal muscle lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted bandsize: 18 kDa Blocking/Dilution buffer: 5%NFDM/TBST.)
EMP1, Polyclonal Antibody (Cat# AAA28735)
Full Name
EMP1 Antibody (N-term)
Gene Names
EMP1; TMP; CL-20; EMP-1
Reactivity
Human
Applications
Western Blot
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cells, using CYCS antibody.)
AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26880)
Full Name
AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (MaxLight 650)
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Mouse, Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using AAA28688 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody)
Ubiquitin, Polyclonal Antibody (Cat# AAA28688)
Full Name
Ubiquitin Antibody (N-term)
Gene Names
UBC; HMG20
Reactivity
Human \Human, Mouse, Rat
Predicted Reactivity: Bovine, Drosophila, Monkey, Pig, Sheep, Chicken, Horse, Hamster, Xenopus, Rabbit
Predicted Reactivity: Bovine, Drosophila, Monkey, Pig, Sheep, Chicken, Horse, Hamster, Xenopus, Rabbit
Applications
Western Blot, Immunohistochemistry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
DB (Dot Blot)
(Dot Blot: 1 ug peptide was blot onto NC membraneA: JAK2 (pY1008, pY1009)B: JAK2 (Non phosphospecific)C: Non-related Phosphopeptide) were blotted at a 1:2000 dilution by:1: Rabbit anti-JAK2(pY1008/pY1009) (AAA14087)2: Rabbit anti-JAK2 (Nonphospho specific).)
JAK2 (pY1007/Y1008), Antibody (Cat# AAA14087)
Full Name
Rabbit anti Phospho- JAK2 (pTyr 1007/1008)
Gene Names
JAK2; JTK10
Reactivity
Human, Rat, Mouse
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
The Rabbit IgG is purified by site-modified Epitope Affinity Purification.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of LPHN2 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
LPHN2, Polyclonal Antibody (Cat# AAA27788)
Full Name
Anti-LPHN2 Antibody
Gene Names
ADGRL2; CL2; LEC1; CIRL2; LPHH1; LPHN2
Reactivity
Human, Mouse, Rat, Bovine
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Vasopressin V2 Receptor staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Vasopressin V2 Receptor, Polyclonal Antibody (Cat# AAA27813)
Full Name
Anti-Vasopressin V2 Receptor Antibody
Gene Names
AVPR2; DI1; DIR; NDI; V2R; ADHR; DIR3
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
FCM (Flow Cytometry)
(PYY Antibody (C-term) (AAA28733) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
PYY, Polyclonal Antibody (Cat# AAA28733)
Full Name
PYY Antibody (C-term)
Gene Names
PYY; PYY1; PYY-I
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with NRK Antibody (Center) (AAA28689). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NRK primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NRK immunoreactivity is localized to Cytoplasm and Nucleus significantly.)
NRK, Polyclonal Antibody (Cat# AAA28689)
Full Name
NRK Antibody (Center)
Gene Names
NRK; NESK
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of SLK staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
SLK, Polyclonal Antibody (Cat# AAA27796)
Full Name
Anti-SLK Antibody
Gene Names
SLK; LOSK; STK2; se20-9; bA16H23.1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Human placenta tissue using AAA28695 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
GAA, Polyclonal Antibody (Cat# AAA28695)
Full Name
GAA Antibody (N-term)
Gene Names
GAA; LYAG
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing Hela cells stained with AAA28750 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28750, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10, 000 events wasperformed.)
WNT5A, Polyclonal Antibody (Cat# AAA28750)
Full Name
WNT5A Antibody (Center)
Gene Names
WNT5A; hWNT5A
Reactivity
Human, Mouse, Rat
Predicted: Rabbit
Predicted: Rabbit
Applications
Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western blot analysis of SLC10A1 using anti- SLC10A1 antibody (AAA11653).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates (positive control), Lane 2: rat kidney tissue lysates, (negative control)After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1 antigen affinity purified polyclonal antibody (AAA11653) at 0.25ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SLC10A1 at approximately 50KD. The expected band size for SLC10A1 is at 38KD.)
SLC10A1, Polyclonal Antibody (Cat# AAA11653)
Full Name
Anti-SLC10A1 Antibody
Gene Names
Slc10a1; Ntcp
Reactivity
Mouse, Rat
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11864)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
Immunofluorescence
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11930)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
WB (Western Blot)
(Western blot analysis of CD298 expression in HEK293T (A), Hela (B), A2780 (C), mouse heart (D), mouse liver (E), rat heart (F), rat liver (G) whole cell lysates.(Predicted band size: 31 kD; Observed band size: 40 kD))
CD298, Polyclonal Antibody (Cat# AAA27791)
Full Name
Anti-CD298 Antibody
Gene Names
ATP1B3; CD298; ATPB-3
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohistochemistry)
P2Y2, Polyclonal Antibody (Cat# AAA14402)
Full Name
P2Y2 Antibody
Gene Names
P2RY2; P2U; HP2U; P2U1; P2UR; P2Y2; P2RU1; P2Y2R
Reactivity
Cat, Human, Mouse, Rat
Applications
Confocal Microscopy, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Pricing
WB (Western Blot)
(Western BlotPositive WB detected in: recombinant proteinAll lanes: csgA Antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 17 kDaObserved band size: 17 kDa)
csgA, Polyclonal Antibody (Cat# AAA27056)
Full Name
csgA Antibody
Gene Names
csgA; agfA; ECK1028; JW1025
Reactivity
E Coli (strain K12)
Applications
Western Blot
Purity
Affinity-chromatography
Pricing
WB (Western Blot)
(Anti-AVPR1A antibody, AAA11596, All Western blottingAll lanes: Anti-AVPR1A(AAA11596) at 0.5ug/mlLane 1: Rat Liver Tissue Lysate at 40ugLane 2: Rat Kidney Tissue Lysate at 40ugPredicted bind size: 47KDObserved bind size: 47KD)
AVPR1A, Polyclonal Antibody (Cat# AAA11596)
Full Name
Anti-AVPR1A antibody
Gene Names
Avpr1a; V1a; AVPR; V1aR
Reactivity
Reacts with: Human, Rat
Predicted to work with: Mouse
Predicted to work with: Mouse
Applications
Western Blot
Purity
Immunogen affinity purified.
Pricing
Application Data
Collagen 1, alpha 1 propeptide, Polyclonal Antibody (Cat# AAA14237)
Full Name
Anti-Collagen I alpha1 Propeptide Sequence
Gene Names
COL1A1; OI4
Reactivity
Human, Mouse, Rat
Expected Reactivity: Bovine, Canine, Chicken, Feline, Finch, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep, Vole, Xenopus
Expected Reactivity: Bovine, Canine, Chicken, Feline, Finch, Goat, Guinea Pig, Hamster, Horse, Non-Human Primate, Rabbit, Sheep, Vole, Xenopus
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purified rabbit serum.
Pricing
IHC (Immunohistochemistry)
(Figure 3. IHC analysis of SFTPA1 using anti- SFTPA1 antibody (AAA11521).SFTPA1 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- SFTPA1 Antibody (AAA11521) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Surfactant Protein A, Polyclonal Antibody (Cat# AAA11521)
Full Name
Polyclonal Anti-SFTPA1 Antibody
Gene Names
Sftpa1; SP-A; Sftp1; Sftp-1
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IP (Immunoprecipitation)
(Immunoprecipitating TUBB in Hela whole cell lysateLane 1: Mouse control IgG instead in Hela whole cell lysate.Lane 2: (2ug) + Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (5ug)For western blotting, the blot was detected at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:5000)
TUBB, Monoclonal Antibody (Cat# AAA28058)
Full Name
TUBB Monoclonal Antibody
Reactivity
Human, Rat, Rabbit, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein A purified
Pricing
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
HRI, Polyclonal Antibody (Cat# AAA28727)
Full Name
HRI Antibody (N-term)
Gene Names
EIF2AK1; HCR; HRI
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12211)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
H1 (A/California/04/2009)(H1N1), Polyclonal Antibody (Cat# AAA13734)
Full Name
Anti-H1 (aa 200-260)(A/California/04/2009)(H1N1)
Applications
Western Blot
Purity
Purity: >95%
Purification: Immunoaffinity chromatography
Purification: Immunoaffinity chromatography
Pricing
IHC (Immunohistchemistry)
(Western blot analysis of lysates from HUVEC and COLO205 cells. The lane on the right is blocked with the synthesized peptide. Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)
FGR, Polyclonal Antibody (Cat# AAA29727)
Full Name
FGR Antibody
Gene Names
FGR; SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antigen Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cells, using CYCS antibody.)
AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26881)
Full Name
AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (MaxLight 750)
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Mouse, Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of paraffin embedded rat thymus sections using AAA14692 at a 1:250 dilution and detected by Alexa-488, which appears fluorescent green. 8-oxo-dG is present in the cytoplasm and nucleus of most but not all cells. Sections were counter-stained with 7-Aminoactinomycin D (7AAD), which labels nuclear DNA. Images were captured at 40X magnification.)
8-Hydroxy-2'-deoxyguanosine, Monoclonal Antibody (Cat# AAA14692)
Full Name
Mouse anti-8-Hydroxy-2'-deoxyguanosine (8-oxo-dG)
Applications
Immunocytochemistry, Immunohistochemistry, Immunofluorescence
Purity
Purified from ascites.
Pricing
WB (Western Blot)
(Positive WB detected in Recombinant proteinAll lanes: L1R antibody at 1:2000Secondary Goat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 26.8 kDabserved band size: 29 kDa)
L1R, Polyclonal Antibody (Cat# AAA27054)
Full Name
Rabbit anti-Vaccinia virus (strain Copenhagen)(VACV) L1R Polyclonal Antibody
Reactivity
Vaccinia virus
Applications
Western Blot
Purity
Protein G
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of BCOX1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
BCOX1, Polyclonal Antibody (Cat# AAA27816)
Full Name
Anti-BCOX1 Antibody
Gene Names
KIAA0100; BCOX; BCOX1; CT101
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Immunogen affinity chromatography
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of SHARP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
SHARP1, Polyclonal Antibody (Cat# AAA27795)
Full Name
Anti-SHARP1 Antibody
Gene Names
BHLHE41; DEC2; hDEC2; BHLHB3; SHARP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Anti-PIAS1 antibody, AAA11605, All Western blottingAll lanes: Anti-PIAS1(AAA11605) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 40ugLane 2: Mouse Testis Tissue Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: MCF-7 Whole Cell Lysate at 40ugLane 6: SKOV Whole Cell Lysate at 40ugPredicted bind size: 72KDObserved bind size: 100KD)
PIAS1, Polyclonal Antibody (Cat# AAA11605)
Full Name
Anti-PIAS1 antibody
Gene Names
PIAS1; GBP; ZMIZ3; DDXBP1; GU/RH-II
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Immunogen affinity purified.
Pricing