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Gene Name
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Application
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733 results for " l type" - showing 700-733
IF (Immunofluorescence)
(AAA31202 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31202 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
GABA A Receptor alpha 5, Polyclonal Antibody (Cat# AAA31202)
Full Name
GABA A Receptor alpha 5 Antibody
Gene Names
GABRA5; EIEE79
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(88%), Chicken(100%)
Predicted Reactivity: Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Dog(88%), Chicken(100%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistchemistry)
(AAA31064 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
PKC theta, Polyclonal Antibody (Cat# AAA31064)
Full Name
Phospho-PKC theta (Ser676) Antibody
Gene Names
PRKCQ; PRKCT; nPKC-theta
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12211)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
IHC (Immunohistochemistry)
(Immunofluorescence staining of HepG2 cells with AAA27007 at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
ZDHHC14, Polyclonal Antibody (Cat# AAA27007)
Full Name
ZDHHC14 Antibody
Gene Names
ZDHHC14; NEW1CP
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)
Dnmt1, Polyclonal Antibody (Cat# AAA31301)
Full Name
Phospho-Dnmt1 (Ser714) Antibody
Gene Names
DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
Peptide affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of lysate from mouse heart tissue lysate, using MYBPC3 Antibody (N-term). AAA28794 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
MYBPC3, Polyclonal Antibody (Cat# AAA28794)
Full Name
MYBPC3 Antibody (N-term)
Gene Names
MYBPC3; FHC; CMH4; CMD1MM; LVNC10; MYBP-C
Reactivity
Human, Mouse, Rat
(Predicted Reactivity: Rat)
(Predicted Reactivity: Rat)
Applications
Immunohistochemistry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
CEA, Native Protein (Cat# AAA14396)
Full Name
CEA Ag(Preservative-free)
Purity
>95% by SDS-PAGE and electrophoresis
Pricing
Application Data
(TNFR2/TNFRSF1B-mediated inhibition of cytotoxicity was Neutralized by Human TNFR2 Antibody. Recombinant Human TNFR2/TNFRSF1B inhibits Recombinant Human TNFa induced cytotoxicity in the L-929 mouse fibroblast cell line. Inhibition of Recombinant Human TNFa (0.25 ng/mL) activity elicited by Recombinant Human TNFR2/TNFRSF1B (1 ug/mL) is neutralized by increasing concentrations of Human TNFR2/TNFRSF1B Monoclonal Antibody. The IC50 is typically 1-3 ug/mL in the presence of the metabolic inhibitor actinomycin D (1 ug/mL).)
TNFR2/CD120b/TNFRSF1B, Monoclonal Antibody (Cat# AAA27733)
Full Name
TNFR2/CD120b/TNFRSF1B Neutralizing Antibody
Gene Names
TNFRSF1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II
Reactivity
Human
Applications
Blocking, Neutralization
Purity
>95% SDS-PAGE
Pricing
IHC (Immunohistochemistry)
(AAA28674 staining GLUL in Human liver tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
GLUL, Polyclonal Antibody (Cat# AAA28674)
Full Name
GLUL Antibody (N-term)
Gene Names
GLUL; GS; GLNS; PIG43; PIG59
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Chicken, Monkey, Pig)
Applications
Western Blot, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(Western blot analysis of lysates from 293T cell line human brain and mouse brain tissue lysate(from left to right), using STRADA Antibody (C-term). AAA28673 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
STRADA, Polyclonal Antibody (Cat# AAA28673)
Full Name
STRADA Antibody (C-term)
Gene Names
STRADA; LYK5; PMSE; Stlk; STRAD; NY-BR-96
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Applications
Western Blot, Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western blot analysis of XIAP phosphorylation expression in Anisomycin treated HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
XIAP, Polyclonal Antibody (Cat# AAA31060)
Full Name
Phospho-XIAP (Ser87) Antibody
Gene Names
XIAP; API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
SLIT2N, Recombinant Protein (Cat# AAA14389)
Full Name
SLIT2N protein
Purity
>98% by SDS-PAGE
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11850)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
Immunohistochemistry, Western Blot
Pricing
WB (Western Blot)
(Western blot analysis of extracts of rat thymus, using T2R38 antibody (AAA27928) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.)
T2R38, Polyclonal Antibody (Cat# AAA27928)
Full Name
Taste receptor type 2 member 38 (T2R38) (PTC bitter taste receptor) (Taste receptor type 2 member 61)
Gene Names
TAS2R38; PTC; T2R38; T2R61
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
IHC (Immunohistchemistry)
(Figure 9 Immunohistochemistry Validation of IRF7 in Human Liver Tissue Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IRF7 antibody (8991) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
IRF7, Polyclonal Antibody (Cat# AAA11032)
Full Name
IRF7 Antibody
Gene Names
IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
IRF7 Antibody is Protein A purified.
Pricing
IF (Immunofluorescence)
(AAA30921 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
CCR5, Polyclonal Antibody (Cat# AAA30921)
Full Name
Phospho-CCR5(Ser349) Antibody
Gene Names
CCR5; CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
WB (Western Blot)
(All lanes : Anti-LAG3 Antibody (Center) at 1:1000 dilutionLane 1: human ovary lysateLane 2: mouse liver lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 57 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
LAG3, Polyclonal Antibody (Cat# AAA28766)
Full Name
LAG3 Antibody (Center)
Gene Names
LAG3; CD223
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(IHC of rat spleen using anti-CD45 antibody and FFPE tissue after heat-induced antigen retrieval. Anti-CD45 Ab at 1:500/DAB detection.)
CD45, Polyclonal Antibody (Cat# AAA13860)
Full Name
CD45 Polyclonal Antibody
Gene Names
PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
Reactivity
Reacts against Human, Rat, Mouse, Canine, and Monkey proteins.
Applications
Western Blot
Pricing
IF (Immunofluorescence)
(LGR5/GPR49 Antibody (loop2) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).)
LGR5/GPR49, Polyclonal Antibody (Cat# AAA28712)
Full Name
LGR5/GPR49 Antibody (loop2)
Gene Names
LGR5; FEX; HG38; GPR49; GPR67; GRP49
Reactivity
Human, mouse, rat
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(DRD2 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DRD2 antibody detected the DRD2 protein (arrow).)
DRD2, Polyclonal Antibody (Cat# AAA28691)
Full Name
DRD2 Antibody (C-term)
Gene Names
DRD2; D2R; D2DR
Reactivity
Human, mouse
Applications
Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of NIH/3T3 cell using MMP16 antibody (AAA28228) at dilution of 1:100. Blue: DAPI for nuclear staining)
MMP16, Polyclonal Antibody (Cat# AAA28228)
Full Name
MMP16 Polyclonal Antibody
Gene Names
MMP16; MMP-X2; C8orf57; MT-MMP2; MT-MMP3; MT3-MMP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Pricing
Application Data
CEA, Native Protein (Cat# AAA14367)
Full Name
CEA protein
Gene Names
CEACAM6; NCA; CEAL; CD66c
Applications
Control
Purity
>95% by SDS-PAGE and electrophoresis
Pricing
WB (Western Blot)
(All lanes : Anti-hDCAMKL1-V705 at 1:8000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lane 3: Rat brain lysate Lane 4: TT whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
DCAMKL1, Polyclonal Antibody (Cat# AAA28779)
Full Name
DCAMKL1 Antibody (C-term)
Gene Names
DCLK1; CL1; DCLK; CLICK1; DCDC3A; DCAMKL1
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(BCORL1 Antibody (N-term) (AAA28773) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
BCORL1, Polyclonal Antibody (Cat# AAA28773)
Full Name
BCORL1 Antibody (N-term)
Gene Names
BCORL1; BCoR-L1; CXorf10
Reactivity
Human
Applications
Flow Cytometry, Western Blot, Immunohistochemistry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western blot analysis of extracts from rat brain, using MMP14 Ab.)
MMP14, Polyclonal Antibody (Cat# AAA30926)
Full Name
MMP14 Antibody
Gene Names
MMP14; MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; WNCHRS; MT1-MMP; MT-MMP 1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
FCM (Flow Cytometry)
(SCNN1A Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SCNN1A, Polyclonal Antibody (Cat# AAA28784)
Full Name
SCNN1A Antibody (Center)
Gene Names
SCNN1A; BESC2; ENaCa; SCNEA; SCNN1; ENaCalpha
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation analysis of extracts of Jurkat cells, using DNMT1 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
DNMT1, Polyclonal Antibody (Cat# AAA28351)
Full Name
DNMT1 Rabbit pAb
Gene Names
DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Chromatin Immunoprecipitation
Purity
Affinity purification
Pricing
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug DNMT1 antibody . Western blot was performed from the immunoprecipitate using DNMT1 antibody at a dilition of 1:1000.)
DNMT1, Polyclonal Antibody (Cat# AAA28335)
Full Name
DNMT1 Rabbit pAb
Gene Names
DNMT1; AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
Affinity purification
Pricing
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human lung tissue using AAA26920 at dilution of 1:100)
60 kDa SS-A/Ro ribonucleoprotein, Polyclonal Antibody (Cat# AAA26920)
Full Name
Rabbit anti-human 60 kDa SS-A/Ro ribonucleoprotein polyclonal Antibody
Gene Names
TROVE2; RO60; SSA2; RORNP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
WB (Western Blot)
(Western blot analysis of extracts from K562 cells, using Collagen XI alph1 antibody.)
Collagen XI alpha1, Polyclonal Antibody (Cat# AAA31109)
Full Name
Collagen XI alpha1 Antibody
Gene Names
COL11A1; STL2; COLL6; CO11A1
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Peptide affinity purification
Pricing
Free Thyroxine, FT4, ELISA Kit (Cat# AAA15023)
Full Name
Mouse Free Thyroxine, FT4 ELISA Kit
Reactivity
Mouse
Pricing
CD105, Antibody (Cat# AAA20025)
Full Name
Anti-Hu CD105 PE
Gene Names
ENG; END; HHT1; ORW1
Reactivity
Pig, Human
Applications
Flow Cytometry
Purity
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Pricing
Standard Curve (Sample)
Tumor Necrosis Factor alpha (TNF-alpha), ELISA Kit (Cat# AAA18946)
Full Name
Hamster Tumor Necrosis Factor alpha (TNF-alpha) ELISA Kit
Reactivity
Hamster
Pricing