Search results for " l type"

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IF (Immunofluorescence) (AAA29758 staining HepG2 cells(4h of LPS treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).)

STING, Polyclonal Antibody (Cat# AAA29758)

Full Name

STING (Phospho-Ser366) Antibody

Gene Names

TMEM173; ERIS; MITA; MPYS; SAVI; NET23; STING; hMITA; hSTING

Reactivity

Human, Mouse

Applications

Westen Blot, Immunohistochemistry, Immunofluorescence

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Pricing

ZNRF3, Polyclonal Antibody (Cat# AAA26914)

Full Name

ZNRF3 Antibody

Gene Names

ZNRF3; RNF203; BK747E2.3

Reactivity

Human

Applications

Immunofluorescence

Purity

>95%, Protein G purified

Pricing

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WB (Western Blot) (SLCO1B3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SLCO1B3 antibody detected the SLCO1B3 protein (arrow).)

SLCO1B3, Polyclonal Antibody (Cat# AAA28785)

Full Name

SLCO1B3 Antibody (C-term)

Gene Names

SLCO1B3; LST3; HBLRR; LST-2; OATP8; OATP-8; OATP1B3; SLC21A8; LST-3TM13

Reactivity

Human

Applications

Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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IF (Immunofluorescence) (AAA31206 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31206) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Activin Receptor Type IA, Polyclonal Antibody (Cat# AAA31206)

Full Name

Activin Receptor Type IA Antibody

Gene Names

ACVR1; FOP; ALK2; SKR1; TSRI; ACTRI; ACVR1A; ACVRLK2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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IHC (Immunohistochemistry) (AAA30985 at 1/50 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Keratin 8, Polyclonal Antibody (Cat# AAA30985)

Full Name

Phospho-Keratin 8 (Ser432) Antibody

Gene Names

KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

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CD45, Monoclonal Antibody (Cat# AAA27049)

Full Name

CD45 Monoclonal Antibody

Gene Names

PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

>95%, Protein A purified

Pricing

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IF (Immunofluorescence) (AAA31071 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

HER4, Polyclonal Antibody (Cat# AAA31071)

Full Name

Phospho-HER4 (Tyr1284) Antibody

Gene Names

ERBB4; HER4; ALS19; p180erbB4

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

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FCM (Flow Cytometry) (WB - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail IHC-P - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail FC - MCSF Receptor (CSF1R) Antibody (C-term) AAA28757 detail Overlay histogram showing HepG2 cells stained with AAA28757(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28757, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)

MCSF Receptor (CSF1R), Polyclonal Antibody (Cat# AAA28757)

Full Name

MCSF Receptor (CSF1R) Antibody (C-term)

Gene Names

CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R

Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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CD40, Monoclonal Antibody (Cat# AAA12090)

Full Name

MOUSE ANTI HUMAN CD40

Gene Names

CD40; p50; Bp50; CDW40; TNFRSF5

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry

Pricing

TIGIT, Polyclonal Antibody (Cat# AAA28803)

Full Name

TIGIT Antibody

Gene Names

TIGIT; VSIG9; VSTM3; WUCAM

Reactivity

Human, Mouse

Applications

Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

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CD40, Monoclonal Antibody (Cat# AAA11867)

Full Name

MOUSE ANTI HUMAN CD40:FITC

Gene Names

CD40; p50; Bp50; CDW40; TNFRSF5

Applications

Flow Cytometry

Pricing

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CD40, Monoclonal Antibody (Cat# AAA11936)

Full Name

MOUSE ANTI HUMAN CD40

Gene Names

CD40; p50; Bp50; CDW40; TNFRSF5

Reactivity

Dog

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Immunohistochemistry

Pricing

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IF (Immunofluorescence) (AAA31173 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31173 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Nav1.8, Polyclonal Antibody (Cat# AAA31173)

Full Name

Nav1.8 Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

Peptide affinity purification

Pricing

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PCSK9, Polyclonal Antibody (Cat# AAA28774)

Full Name

PCSK9 Antibody (N-term)

Gene Names

PCSK9; FH3; PC9; NARC1; LDLCQ1; NARC-1; HCHOLA3

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Pricing

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CD40, Monoclonal Antibody (Cat# AAA12089)

Full Name

MOUSE ANTI HUMAN CD40:RPE

Gene Names

CD40; p50; Bp50; CDW40; TNFRSF5

Applications

Flow Cytometry

Pricing

ZDHHC9, Polyclonal Antibody (Cat# AAA28212)

Full Name

ZDHHC9 Polyclonal Antibody

Gene Names

ZDHHC9; CGI89; DHHC9; MMSA1; MRXSZ; ZNF379; ZNF380; CXorf11; ZDHHC10

Applications

Western Blot

Purity

Affinity Purification

Pricing

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IHC (Immunohistchemistry) (AAA31073 at 1/100 staining Carcinoid tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 107°C.)

SIRT3, Polyclonal Antibody (Cat# AAA31073)

Full Name

SIRT3 Antibody

Gene Names

SIRT3; SIR2L3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Peptide ELISA

Purity

Peptide affinity purification

Pricing

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

IRF7, Polyclonal Antibody (Cat# AAA31330)

Full Name

Phospho-IRF7 (Ser471/Ser472) Antibody

Gene Names

IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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CD40, Monoclonal Antibody (Cat# AAA12031)

Full Name

MOUSE ANTI HUMAN CD40:RPE

Gene Names

CD40; p50; Bp50; CDW40; TNFRSF5

Applications

Flow Cytometry

Pricing

TNFR1/CD120a/TNFRSF1A, Monoclonal Antibody (Cat# AAA27740)

Full Name

TNFR1/CD120a/TNFRSF1A Neutralizing Antibody

Gene Names

TNFRSF1A; FPF; MS5; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; TNFR1-d2

Applications

Neutralization

Pricing

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Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

Application Data (Published customer image: Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A') The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A'). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 um; D-F, I-K, N-P: 200 um; Q: 100 um; R, S: 10 um; T: 50 um; U, V, W: 25 um.From: Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. Dayraud, CC. et al. BMC Evolutionary Biology 2012, 12:107.)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12232)

Full Name

RAT ANTI TUBULIN ALPHA:HRP

Applications

Immunohistochemistry, Western Blot

Pricing

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Application Data (Published customer image: Spindle abnormalities in embryos derived from imp-a2D14/imp-betaKetRE34 and imp-a2D14/imp-betac02473; NLSB-/+ females. (A -D) Wild-type and mutant embryos stained for a-tubulin (green) and DNA (blue). (A) Mitotic spindles in wild-type embryos at metaphase and anaphase. (B, C) Categories of spindle abnormalities found in embryos derived from (B) imp-a2D14/imp-betaKetRE34 and (C) imp-a2D14/imp-betac02743; NLSB-/+ females. (D) Formation of aster networks found in both genotypes. Scale bar: 10 um. (E) Frequency of spindle defects in embryos from both types of mutant females. Female genotypes are displayed at the upper right corner. At least 200 spindles were scored for both genotypes.From: Specific Cooperation Between Imp-a2 and Imp-beta/Ketel in Spindle Assembly During Drosophila Early Nuclear Divisions Erika Vir¡gh, M¡ty¡s Gorj¡n¡cz, Istv¡n T¶r¶k, Tolga Eichhorn, Sowjanya Kallakuri, Tam¡s Szlanka, Istv¡n Kiss, and Bernard M. Mechler G3 January 2012 2:1-14.)

Application Data (Published customer image: Expression of PpiMHCIIa, PpiMHCIIb1 and PpiMHCIIb2 in the tentacular apparatus. (A) Schematic drawing of the tentacle root in internal view. The three coloured horizontal lines materialise the three different planes of transverse cryosections corresponding to panels D, I, N (in green), E, J, O (in orange) and F, K, P (in purple). (A') The tentacle root in external view (corresponding to the side of tentacle insertion). The dotted lines delineate the longitudinal dissections to remove tentacle root lateral expansions in order to obtain the preparations shown in (C, H, M). (A) Schematic drawing of the tentacle root in longitudinal section, after removal of the lateral expansions following the dotted lines in (A'). Horizontal lines indicating the three planes of cryosections as in (A). (B, G, L) Internal views of isolated tentacle roots showing PpiMHCIIa (B), PpiMHCIIb1 (G), PpiMHCIIb2 (L) expressions. (C, H, M) Longitudinal sections of the tentacle root stained with PpiMHCIIa (C), PpiMHCIIb1 (H), PpiMHCIIb2 (M) anti-sense probes. The aboral pole is at the top in panels (B, C, G, H, L, M). (D-F, I-K, N-P) Transverse cryosections of whole-mount ISH for the three genes, with sectioning plane indicated by the colour of the surrounding line according to the colour code outlined in panel (A). The black arrowhead in (G-J) points to a stained line above the median ridge which appears to be more or less in continuity with the two layered bands labelled M Tcl. (Q) YL1/2 (anti-tyrosylated-a-tubulin, in red) and DAPI (in blue) counterstaining of the region boxed in (N). (R) Higher magnification of the region indicated by the box in (Q). (S) YL1/2 (red) and DAPI (blue) counterstaining of the region boxed in (P). (T-W) Expression of PpiMHCIIb1 gene in tentillae. (T) Whole mount ISH of tentacle and tentillae. (U) Higher magnification view of the region boxed in (T). (V) Transverse cryosection of whole-mount ISH of a tentilla. Two symmetrical muscle fibres are stained. (W) YL1/2 (in red) and DAPI (in blue) counterstaining of (V). The YL1/2 staining in colloblasts is certainly due to non-specific fixation of the antibody on the sticky colloblast granules. (X) Schematic drawing of a tentilla in transverse section. Coll: Colloblasts; F tt: Forming tentillae; LR: Lateral Ridge; MR: Median Ridge; M tcl: Tentacle Muscle progenitors; M tt: Tentilla muscle progenitors; Mu: Muscle fibres; N Tcl: Tentacle Neural cells; N tt: Tentilla Neural cells Tcl: Tentacle; Tt: Tentilla. Scale bars: B, C, G, H, L, M: 100 um; D-F, I-K, N-P: 200 um; Q: 100 um; R, S: 10 um; T: 50 um; U, V, W: 25 um.From: Independent specialisation of myosin II paralogues in muscle vs. non-muscle functions during early animal evolution: a ctenophore perspective. Dayraud, CC. et al. BMC Evolutionary Biology 2012, 12:107.)

TUBULIN ALPHA, Monoclonal Antibody (Cat# AAA12009)

Full Name

RAT ANTI TUBULIN ALPHA

Applications

Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Radioimmunoassay, Western Blot

Pricing

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Application Data (Sandwich ELISA analysis of CD178 binding using Mouse anti Human CD178 as a capture reagent and biotinylated Mouse anti Human CD178 as a detection reagent with purified human CD178 as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . Plasma (orange) sample is displayed at 1:2 dilution.)

CD178, Monoclonal Antibody (Cat# AAA11962)

Full Name

MOUSE ANTI HUMAN CD178

Gene Names

FASLG; APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1

Reactivity

Human

Applications

Flow Cytometry, Immunoprecipitation

Pricing

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V5-TAG, Monoclonal Antibody (Cat# AAA12081)

Full Name

MOUSE ANTI V5-TAG:HRP

Applications

Western Blot

Pricing

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IHC (Immunohistchemistry) (At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Ephrin B1/B2/B3, Polyclonal Antibody (Cat# AAA31292)

Full Name

Phospho-Ephrin B1/B2/B3 (Tyr324) Antibody

Gene Names

EFNB1; CFND; CFNS; EFL3; EPLG2; Elk-L; LERK2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Peptide ELISA

Purity

Peptide affinity purification

Pricing

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IF (Immunofluorescence) (AAA31177 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31177 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

Proteasome 20S LMP7, Polyclonal Antibody (Cat# AAA31177)

Full Name

Proteasome 20S LMP7 Antibody

Gene Names

PSMB8; JMP; ALDD; LMP7; NKJO; D6S216; PRAAS1; PSMB5i; RING10; D6S216E

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Horse(86%), Rabbit(100%), Dog(88%)

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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WB (Western Blot) (HHLA2 Antibody (N-term) western blot analysis in MDA-MB453,CEM,HL-60,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HHLA2 antibody detected the HHLA2 protein (arrow).)

HHLA2, Polyclonal Antibody (Cat# AAA28722)

Full Name

HHLA2 Antibody (N-term)

Gene Names

HHLA2; B7H7; B7-H7

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

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CD163, Monoclonal Antibody (Cat# AAA11943)

Full Name

MOUSE ANTI HUMAN CD163

Gene Names

CD163; M130; MM130

Reactivity

Guinea Pig, Pig, Rhesus Monkey, Sheep

Applications

Immunohistochemistry, Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoassay, Western Blot

Pricing

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Histone H2B, Polyclonal Antibody (Cat# AAA31352)

Full Name

Acetyl-Histone H2B (Lys20) Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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IF (Immunofluorescence) (Immunofluorescence staining of Hela cell with AAA26999 at 1:90, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

SLC7A6, Polyclonal Antibody (Cat# AAA26999)

Full Name

SLC7A6 Antibody

Gene Names

SLC7A6; LAT3; LAT-2; y+LAT-2

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

>95%, Protein G purified

Pricing

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TAS1R3 antibody (AAA28219) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1s.)

TAS1R3, Polyclonal Antibody (Cat# AAA28219)

Full Name

TAS1R3 Polyclonal Antibody

Gene Names

TAS1R3; T1R3

Applications

Western Blot

Purity

Affinity purification

Pricing

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IF (Immunofluorescence) (Immunofluorescent analysis of HepG2 cells using AAA26930 at a dilution of 1:100 and Alexa Fluor 489-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Lysosomal acid lipase/cholesteryl ester hydrolase, Polyclonal Antibody (Cat# AAA26930)

Full Name

Rabbit anti-human Lysosomal acid lipase/cholesteryl ester hydrolase polyclonal Antibody

Gene Names

LIPA; LAL; CESD

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

>95%, Protein G purified

Pricing

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Application Data (AAA13666 (1.5ug/ml) as the reporter with EB002025 as the capture rabbit antibody (5ug/ml).)

FTL, Polyclonal Antibody (Cat# AAA13666)

Full Name

Goat anti-FTL Antibody

Gene Names

FTL; LFTD; NBIA3

Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

Applications

Peptide ELISA, Western Blot, Immunohistochemistry

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

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RECQL5, Polyclonal Antibody (Cat# AAA31264)

Full Name

RECQL5 Antibody

Gene Names

RECQL5; RECQ5

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig(86%)

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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WB (Western Blot) (Western blot analysis of Phospho-PKC-pan (Thr497) expression in various lysates)

PKC-pan, Polyclonal Antibody (Cat# AAA31017)

Full Name

Phospho-PKC-pan (Thr497) Antibody

Gene Names

PRKCA; AAG6; PKCA; PRKACA; PKCI+/-; PKCalpha; PKC-alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

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WB (Western Blot) (Western blot analysis of n-NOS phosphorylation expression in A549 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

n-NOS, Polyclonal Antibody (Cat# AAA31028)

Full Name

Phospho-n-NOS (Ser852) Antibody

Gene Names

NOS1; NOS; bNOS; nNOS; IHPS1; N-NOS; NC-NOS

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

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Smad3, Polyclonal Antibody (Cat# AAA31434)

Full Name

Phospho-Smad3 (Ser423+Ser425) Antibody

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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OSR2, Polyclonal Antibody (Cat# AAA31112)

Full Name

OSR2 Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

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IF (Immunofluorescence) (AAA31227 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25Â°C. Samples were then incubated with primary Ab(AAA31227 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37Â°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

SET, Polyclonal Antibody (Cat# AAA31227)

Full Name

SET Antibody

Gene Names

SET; 2PP2A; IGAAD; MRD58; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA

Reactivity

Human, Rat

Applications

ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).

Pricing

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WB (Western Blot) (Western blot analysis of Phospho-IL-8R beta/CDw128 beta (Ser347) expression in Mouse kidney lysate)

IL-8R beta/CDw128 beta, Polyclonal Antibody (Cat# AAA31026)

Full Name

Phospho-IL-8R beta/CDw128 beta (Ser347) Antibody

Gene Names

CXCR2; CD182; IL8R2; IL8RA; IL8RB; CMKAR2; CDw128b

Reactivity

Human, Mouse, Monkey

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

SLC7A5 / CD98, Polyclonal Antibody (Cat# AAA12328)

Full Name

SLC7A5 / CD98 Light Chain

Gene Names

SLC7A5; E16; CD98; LAT1; 4F2LC; MPE16; hLAT1; D16S469E

Reactivity

Human, Monkey, Dog.
Predicted: Bat, Rabbit

Applications

Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

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PD-L1, Monoclonal Antibody (Cat# AAA27016)

Full Name

PD-L1 Monoclonal Antibody

Gene Names

CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry

Purity

>95%, Protein G purified

Pricing

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

eNOS, Polyclonal Antibody (Cat# AAA31394)

Full Name

Phospho-eNOS (Ser1179) Antibody

Gene Names

NOS3; eNOS; ECNOS

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Rabbit (100%), Dog (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of 293T cells using anti-ARG2 antibody (AAA19256).Overlay histogram showing 293T cells stained with AAA19256 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ARG2 Antibody (AAA19256, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ARG2, Polyclonal Antibody (Cat# AAA19256)

Full Name

Anti-ARG2 Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

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IF (Immunofluorescence) (AAA31098 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AAA31098 1:200) and mouse antibeta tubulin Ab for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)

Piezo1, Polyclonal Antibody (Cat# AAA31098)

Full Name

Piezo1 Antibody

Gene Names

PIEZO1; DHS; Mib; LMPH3; FAM38A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

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WB (Western Blot) (Western blotAll lanes: CXCR4 antibody at 2ug/ml+Hela cellsSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 40,41 kDaObserved band size: 40 kDa)

CXCR4, Polyclonal Antibody (Cat# AAA26937)

Full Name

CXCR4 Antibody

Gene Names

CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; WHIMS; HSY3RR; NPYY3R; D2S201E

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

>95%, Protein G purified

Pricing

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FCM (Flow Cytometry) (Overlay histogram showing Hela cells stained with AAA28750 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28750, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (1583138) at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x106 cells) used under the same conditions. Acquisition of >10, 000 events wasperformed.)