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Application
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797 results for " Synthetic Protein" - showing 700-750
IHC (Immunohistchemistry)
(Western blot analysis of lysates from HUVEC and COLO205 cells. The lane on the right is blocked with the synthesized peptide. Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)
FGR, Polyclonal Antibody (Cat# AAA29727)
Full Name
FGR Antibody
Gene Names
FGR; SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antigen Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts of various cells, using CYCS antibody.)
AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26881)
Full Name
AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (MaxLight 750)
Gene Names
ACACA; ACC; ACAC; ACC1; ACCA; ACACAD
Reactivity
Mouse, Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Positive WB detected in Recombinant proteinAll lanes: L1R antibody at 1:2000Secondary Goat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 26.8 kDabserved band size: 29 kDa)
L1R, Polyclonal Antibody (Cat# AAA27054)
Full Name
Rabbit anti-Vaccinia virus (strain Copenhagen)(VACV) L1R Polyclonal Antibody
Reactivity
Vaccinia virus
Applications
Western Blot
Purity
Protein G
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of BCOX1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
BCOX1, Polyclonal Antibody (Cat# AAA27816)
Full Name
Anti-BCOX1 Antibody
Gene Names
KIAA0100; BCOX; BCOX1; CT101
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
Immunogen affinity chromatography
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of SHARP1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
SHARP1, Polyclonal Antibody (Cat# AAA27795)
Full Name
Anti-SHARP1 Antibody
Gene Names
BHLHE41; DEC2; hDEC2; BHLHB3; SHARP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Anti-PIAS1 antibody, AAA11605, All Western blottingAll lanes: Anti-PIAS1(AAA11605) at 0.5ug/mlLane 1: Rat Testis Tissue Lysate at 40ugLane 2: Mouse Testis Tissue Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: JURKAT Whole Cell Lysate at 40ugLane 5: MCF-7 Whole Cell Lysate at 40ugLane 6: SKOV Whole Cell Lysate at 40ugPredicted bind size: 72KDObserved bind size: 100KD)
PIAS1, Polyclonal Antibody (Cat# AAA11605)
Full Name
Anti-PIAS1 antibody
Gene Names
PIAS1; GBP; ZMIZ3; DDXBP1; GU/RH-II
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(WB of BTBD3 antibody (AAA14405) with mouse kidney. Antibody dilution 1:500 in DiluOBuffer.Apparent MW of BTBD3 varients are 46, 56, and 64 kDa.)
BTBD3, Polyclonal Antibody (Cat# AAA14405)
Full Name
BTBD3 Antibody
Gene Names
BTBD3; dJ742J24.1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using AAA28679 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
HK1 (Hexokinase), Polyclonal Antibody (Cat# AAA28679)
Full Name
HK1 (Hexokinase) Antibody (N-term)
Gene Names
HK1; HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(All lanes : Anti-ERVK-7 Antibody (N-Term) at 1:1000-1:2000 dilutionLane 1: Raji whole cell lysateLane 2: human lung lysateLane 3: human testis lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 67 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
ERVK-7, Polyclonal Antibody (Cat# AAA28797)
Full Name
ERVK-7 Antibody (N-Term)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
ISG15, Polyclonal Antibody (Cat# AAA23589)
Full Name
ISG15 antibody - middle region
Gene Names
ISG15; G1P2; IP17; UCRP; IFI15; IMD38; hUCRP
Reactivity
Tested Reactivity: Human, Mouse
Predicted Reactivity: Cow, Dog, Goat, Horse, Human, Pig, Rabbit, Sheep
Predicted Reactivity: Cow, Dog, Goat, Horse, Human, Pig, Rabbit, Sheep
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 antibody (N-term) (Cat.#AAA28756), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
EN1 (Engrailed 1), Polyclonal Antibody (Cat# AAA28756)
Full Name
EN1 (Engrailed 1) Antibody (N-term)
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
Application Data
SAPOSIN (PSAP), Antibody (Cat# AAA14090)
Full Name
Rabbit anti Saposin antibody
Reactivity
Human
Applications
Western Blot
Purity
The Rabbit IgG is purified by specific Epitope Affinity Purification.
Pricing
WB (Western Blot)
(Western blot analysis of Beta-3 Adrenergic Receptor expression in HEK293T (A), Hela (B), HepG2 (C), A2780 (D), mouse kidney (E), rat liver (F) whole cell lysates.)
Beta-3 Adrenergic Receptor, Polyclonal Antibody (Cat# AAA27792)
Full Name
Anti-Beta-3 Adrenergic Receptor Antibody
Gene Names
ADRB3; BETA3AR
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
DB (Dot Blot)
(Dot blot analysis of anti-AKT3-pS472 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Phospho-AKT3 (S472), Polyclonal Antibody (Cat# AAA28777)
Full Name
Phospho-AKT3 (S472) Antibody
Gene Names
AKT3; MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma
Reactivity
Human
Predicted: Mouse, Rat.
Predicted: Mouse, Rat.
Applications
Dot Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using AAA29759 (L1RE1 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200))
L1RE1, Polyclonal Antibody (Cat# AAA29759)
Full Name
L1RE1 Antibody
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Pricing
IHC (Immunohistochemistry)
(The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using CNTN1 Antibody at dilution 1/100, on the right is treated with synthetic peptide. (Original magnification: x200))
CNTN1, Polyclonal Antibody (Cat# AAA29730)
Full Name
CNTN1 Antibody
Gene Names
CNTN1; F3; GP135; MYPCN
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Antigen affinity purification
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of U-2OS cells using RAB7A antibody (A12784) at dilution of 1:100. Blue: DAPI for nuclear staining.)
RAB7A, Polyclonal Antibody (Cat# AAA28256)
Full Name
RAB7A Polyclonal Antibody
Gene Names
RAB7A; RAB7; PRO2706
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunohistochemistry
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
(AAA28674 staining GLUL in Human liver tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
GLUL, Polyclonal Antibody (Cat# AAA28674)
Full Name
GLUL Antibody (N-term)
Gene Names
GLUL; GS; GLNS; PIG43; PIG59
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Chicken, Monkey, Pig)
Applications
Western Blot, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(ENAM Antibody (C-term) (AAA28699) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
ENAM, Polyclonal Antibody (Cat# AAA28699)
Full Name
ENAM Antibody (C-term)
Gene Names
ENAM; ADAI; AI1C; AIH2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Aquaporin 4 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Aquaporin 4, Monoclonal Antibody (Cat# AAA27803)
Full Name
Anti-Aquaporin 4 Antibody
Gene Names
AQP4; MIWC; WCH4
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Pricing
IHC (Immunohistochemistry)
(Western blot analysis of lysates from 293T cell line human brain and mouse brain tissue lysate(from left to right), using STRADA Antibody (C-term). AAA28673 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
STRADA, Polyclonal Antibody (Cat# AAA28673)
Full Name
STRADA Antibody (C-term)
Gene Names
STRADA; LYK5; PMSE; Stlk; STRAD; NY-BR-96
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Applications
Western Blot, Flow Cytometry, Immunofluorescence, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Western blot analysis of lysate from mouse heart tissue lysate, using MYBPC3 Antibody (N-term). AAA28794 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
MYBPC3, Polyclonal Antibody (Cat# AAA28794)
Full Name
MYBPC3 Antibody (N-term)
Gene Names
MYBPC3; FHC; CMH4; CMD1MM; LVNC10; MYBP-C
Reactivity
Human, Mouse, Rat
(Predicted Reactivity: Rat)
(Predicted Reactivity: Rat)
Applications
Immunohistochemistry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
FCM (Flow Cytometry)
(RSPO2 Antibody (C-term) (AAA28708) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
RSPO2, Polyclonal Antibody (Cat# AAA28708)
Full Name
RSPO2 Antibody (C-term)
Gene Names
RSPO2; CRISTIN2
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Westen Blot, Immunohistochemistry, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Western blot analysis of LoVo cell lysate, using PIBF1 Antibody. The lane on the left is treated with the antigen-specific peptide.)
PIBF1, Polyclonal Antibody (Cat# AAA31130)
Full Name
PIBF1 Antibody
Gene Names
PIBF1; PIBF; CEP90; JBTS33; C13orf24
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
AOC3 / VAP-1, Polyclonal Antibody (Cat# AAA12319)
Full Name
Goat Polyclonal to Human AOC3 / VAP-1
Gene Names
AOC3; HPAO; SSAO; VAP1; VAP-1
Reactivity
Human, Hamster
Predicted Reactivity: Monkey, Mouse, Rat, Rabbit
Predicted Reactivity: Monkey, Mouse, Rat, Rabbit
Applications
Immunohistochemistry, Immunohistochemistry, Peptide ELISA
Purity
Immunoaffinity Purified
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11850)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
Immunohistochemistry, Western Blot
Pricing
IHC (Immunohistochemistry)
(The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using AAA29762(FIZ1 Antibody) at dilution 1/35.(Original magnification: 200))
FIZ1, Polyclonal Antibody (Cat# AAA29762)
Full Name
FIZ1 Antibody
Gene Names
FIZ1; ZNF798
Reactivity
Human, Mouse
Applications
Immunohistochemistry
Purity
Antigen affinity purification
Pricing
WB (Western Blot)
(Western blot analysis of Arylsulfatase B expression in HEK293T (A), NS-1 (B), H9C2 (C) whole cell lysates.)
Arylsulfatase B, Polyclonal Antibody (Cat# AAA27789)
Full Name
Anti-Arylsulfatase B Antibody
Gene Names
ARSB; ASB; G4S; MPS6
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment.)
RIPK3, Polyclonal Antibody (Cat# AAA23385)
Full Name
RIPK3 antibody - N-terminal region
Gene Names
RIPK3; RIP3
Reactivity
Tested Species Reactivty: Human
Predicted Species Reactivity: Cow, Dog, Horse, Human, Mouse, Pig, Rat
Predicted Species Reactivity: Cow, Dog, Horse, Human, Mouse, Pig, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Protein A purified
Pricing
IHC (Immunohistochemistry)
(Anti-5HT2A Receptor antibody, AAA11510, IHC(P)IHC(P): Rat Brain Tissue)
5HT2A Receptor, Polyclonal Antibody (Cat# AAA11510)
Full Name
Anti-5HT2A Receptor antibody
Gene Names
HTR2A; HTR2; 5-HT2A
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Anti-5HT1A Receptor antibody, AAA11520, IHC(P)IHC(P): Rat Brain Tissue)
5HT1A Receptor, Polyclonal Antibody (Cat# AAA11520)
Full Name
Anti-5HT1A Receptor antibody
Gene Names
HTR1A; G-21; 5HT1a; PFMCD; 5-HT1A; 5-HT-1A; ADRBRL1; ADRB2RL1
Reactivity
Reacts with: Human, Rat
Predicted to work with: Mouse
Predicted to work with: Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Cytokeratin 20 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Cytokeratin 20, Polyclonal Antibody (Cat# AAA27786)
Full Name
Anti-Cytokeratin 20 Antibody
Gene Names
KRT20; K20; CD20; CK20; CK-20; KRT21
Reactivity
Human, Mouse, Rat, Bovine, Pig
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Anti-SLC6A19 Antibody (C-Term) at 1:2000 dilution + 293 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 71 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
SLC6A19, Polyclonal Antibody (Cat# AAA28802)
Full Name
SLC6A19 Antibody (C-Term)
Gene Names
SLC6A19; HND; B0AT1
Reactivity
Human
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(Anti- TGM2 Picoband antibody, AAA11646, IHC(F)IHC(F): Human Placenta Tissue)
TGM2, Polyclonal Antibody (Cat# AAA11646)
Full Name
Anti-TGM2 Antibody
Gene Names
TGM2; TGC; TG(C)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
Application Data
(Overlay histogram showing U-2OS cells stained with AP10510c (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated n 2% bovine serum albumin to block non-specificprotein-protein interactions followed by the antibody (AP10510c, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used wasGoat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1?g/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events wasperformed)
CF150, Polyclonal Antibody (Cat# AAA28789)
Full Name
CF150 Antibody (Center)
Gene Names
MB21D1; cGAS; h-cGAS; C6orf150
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immmunohistochemistry)
(PLA2G7 Antibody (Center) (Cat. #AAA28698)IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PLA2G7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
PLA2G7, Polyclonal Antibody (Cat# AAA28698)
Full Name
PLA2G7 Antibody (Center)
Gene Names
PLA2G7; PAFAD; PAFAH; LP-PLA2; LDL-PLA2
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(PLA2G2D Antibody (C-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
PLA2G2D, Polyclonal Antibody (Cat# AAA28718)
Full Name
PLA2G2D Antibody (C-term)
Gene Names
PLA2G2D; SPLASH; sPLA2S; PLA2IID; sPLA2-IID
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
Calponin, Polyclonal Antibody (Cat# AAA14404)
Full Name
Calponin Antibody
Gene Names
CNN1; SMCC; Sm-Calp; HEL-S-14
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Pricing
Application Data
MMP-9, Polyclonal Antibody (Cat# AAA14085)
Full Name
Rabbit anti MMP-9 Polyclonal antibody
Gene Names
MMP9; GELB; CLG4B; MMP-9; MANDP2
Reactivity
Human, Rat, Mouse, Chicken, Dog
Applications
Western Blot, Immunohistochemistry
Purity
The Rabbit IgG is purified by Epitope Affinity Purification.
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing 293F transfected cells stained with AAA27030 (red line) at 1:100. The cells were incubated in 1x PBS/10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
HA-Tag, Monoclonal Antibody (Cat# AAA27030)
Full Name
HA-Tag Monoclonal Antibody
Reactivity
All
Applications
Western Blot, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein A purified
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Human heart tissue using (AAA28740) performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
COL5A1, Polyclonal Antibody (Cat# AAA28740)
Full Name
COL5A1 Antibody (N-term)
Gene Names
COL5A1; EDSC
Reactivity
Human, mouse
Applications
Immunohistochemistry, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistchemistry)
(Immunohistochemical analysis of paraffin-embedded Human kidney. 1, Antibody was diluted at 1:200(4° overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min))
MET, Polyclonal Antibody (Cat# AAA29724)
Full Name
MET Antibody
Gene Names
MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antigen Affinity Purification
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/25.)
CD63, Polyclonal Antibody (Cat# AAA29720)
Full Name
CD63 Antibody
Gene Names
CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of Collagen 2 alpha 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Collagen 2 alpha 1, Polyclonal Antibody (Cat# AAA27787)
Full Name
Anti-Collagen 2 alpha 1 Antibody
Gene Names
COL2A1; AOM; ANFH; SEDC; STL1; COL11A3
Reactivity
Human, Mouse, Rat, Bovine, Dog, Porcine, Rabbit
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
IHC (Immunohistochemistry)
(IHC: Human ovary carcinoma stained with Anti-Legumain (AAA14072) 1:200 dilution. Staining of formalin-fixed tissue requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Legumain, Polyclonal Antibody (Cat# AAA14072)
Full Name
Rabbit anti Legumain Polyclonal Antibody
Gene Names
LGMN; AEP; LGMN1; PRSC1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Epitope Affinity Purification
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Ryanodine Receptor 2 (phospho-Ser2808) staining in U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Ryanodine Receptor 2 (phospho-Ser2808), Polyclonal Antibody (Cat# AAA29761)
Full Name
Ryanodine Receptor 2 (phospho-Ser2808) Antibody
Gene Names
RYR2; RyR; ARVC2; ARVD2; RYR-2; VTSIP
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Immunogen affinity chromatography
Pricing
WB (Western Blot)
(CHAK1 Antibody (AAA28787) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the CHAK1 antibody detected the CHAK1 protein (arrow).)
TRPM7 (CHAK1), Polyclonal Antibody (Cat# AAA28787)
Full Name
TRPM7 (CHAK1) Antibody (C-term)
Gene Names
TRPM7; CHAK; CHAK1; ALSPDC; LTRPC7; LTrpC-7; TRP-PLIK
Reactivity
Human, Mouse
Predicted: Rat
Predicted: Rat
Applications
Western Blot, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(Anti- SFTP A1/2 Picoband antibody, AAA11644, IHC(F)IHC(F): Mouse Lung Tissue)
SFTPA1/2, Polyclonal Antibody (Cat# AAA11644)
Full Name
Anti-SFTPA1/2 Antibody
Gene Names
SFTPA2; PSAP; PSPA; SP-A; SPA2; PSP-A; SFTP1; SPAII; COLEC5; SFTPA2B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen Affinity Purified
Pricing
FCM (Flow Cytometry)
(SERPINH1 Antibody (Center) (Cat. #AAA28730) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SERPINH1, Polyclonal Antibody (Cat# AAA28730)
Full Name
SERPINH1 Antibody (Center)
Gene Names
SERPINH1; CBP1; CBP2; OI10; gp46; AsTP3; HSP47; PIG14; PPROM; RA-A47; SERPINH2
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(TMPRSS2 Antibody (N-term) (RB18784) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TMPRSS2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
TMPRSS2, Polyclonal Antibody (Cat# AAA28791)
Full Name
TMPRSS2 Antibody (N-term)
Gene Names
TMPRSS2; PP9284; PRSS10
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing