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Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
658 results for "Tag Control Antibody" - showing 600-650
FCM (Flow Cytometry)
(FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)
STIP1, Monoclonal Antibody (Cat# AAA26541)
Full Name
STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (HRP)
Gene Names
STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Pricing
FCM (Flow Cytometry)
(FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)
STIP1, Monoclonal Antibody (Cat# AAA26009)
Full Name
STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (AP)
Gene Names
STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Pricing
ADPGK, Monoclonal Antibody (Cat# AAA24419)
Full Name
ADPGK (ADP-dependent Glucokinase, ADP-GK, RbBP-35, PSEC0260, 2610017G09Rik, DKFZp434B195) APC
Gene Names
ADPGK; ADP-GK; 2610017G09Rik
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
TCF19, Monoclonal Antibody (Cat# AAA24385)
Full Name
TCF19 (Transcription Factor 19, TCF-19, SC1, SC1-1, Transcription Factor SC1) (AP)
Gene Names
TCF19; SC1; TCF-19
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
ABL2, Monoclonal Antibody (Cat# AAA24411)
Full Name
ABL2 (Tyrosine-protein Kinase ABL2, Abelson Murine Leukemia Viral Oncogene Homolog 2, Abelson-related Gene Protein, Tyrosine Kinase ARG, ABLL, ARG) APC
Gene Names
ABL2; ARG; ABLL
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
CBS, Monoclonal Antibody (Cat# AAA24452)
Full Name
CBS (Cystathionine beta-synthase, Serine Sulfhydrase, Beta-thionase) APC
Gene Names
CBS; HIP4
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
CAPNS1, Monoclonal Antibody (Cat# AAA24156)
Full Name
CAPNS1 (Calpain Small Subunit 1, CSS1, Calcium-activated Neutral Proteinase Small Subunit, CANP Small Subunit, Calcium-dependent Protease Small Subunit, CDPS, Calcium-dependent Protease Small Subunit 1, Calpain Regulatory Subunit, CAPN4, CAPNS) (AP)
Gene Names
CAPNS1; CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
CAMKIV, Monoclonal Antibody (Cat# AAA24153)
Full Name
CAMKIV (Calcium/Calmodulin-dependent Protein Kinase Type IV, CAM Kinase-GR, CaMK IV, CAMK4) (AP)
Gene Names
CAMK4; caMK; CaMKIV; CaMK IV; CaMK-GR
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
ARNT, Monoclonal Antibody (Cat# AAA24140)
Full Name
ARNT (Aryl Hydrocarbon Receptor Nuclear Translocator, ARNT Protein, Class E Basic Helix-loop-helix Protein 2, bHLHe2, Dioxin Receptor, Nuclear Translocator, Hypoxia-inducible Factor 1-beta, HIF-1-beta, HIF1-beta, BHLHE2) (AP)
Gene Names
ARNT; HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
RAD18, Monoclonal Antibody (Cat# AAA24334)
Full Name
RAD18 (E3 Ubiquitin-protein Ligase RAD18, Postreplication Repair Protein RAD18, hRAD18, hHR18, RING Finger Protein 73, RNF73) (AP)
Gene Names
RAD18; RNF73
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
IF (Immunofluorescence)
(Immunofluorescence analysis of 293T cells using Mouse anti His-Tag mAb (AAA10622) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)
His-Tag, Antibody (Cat# AAA10622)
Full Name
His-Tag Monoclonal Antibody
Applications
Western Blot, Immunoprecipitation
Purity
Affinity Purification
Pricing
ABCF2, Monoclonal Antibody (Cat# AAA24409)
Full Name
ABCF2 (ATP-binding Cassette Sub-family F Member 2, Iron-inhibited ABC Transporter 2, HUSSY-18, DKFZp586K1823, EST133090, M-ABC1) APC
Gene Names
ABCF2; ABC28; HUSSY18; HUSSY-18; EST133090
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
TGIF2, Monoclonal Antibody (Cat# AAA24386)
Full Name
TGIF2 (TGF-beta-induced Transcription Factor 2, TGFB-induced Factor 2, Homeobox Protein TGIF2, 5'-TG-3'-interacting Factor 2) (AP)
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
UGP2, Monoclonal Antibody (Cat# AAA24401)
Full Name
UGP2 (UTP--glucose-1-phosphate Uridylyltransferase, UDP-glucose Pyrophosphorylase, UDPGP, UGPase, UGP1) (AP)
Gene Names
UGP2; UDPG; UGP1; UDPGP; UGPP1; UGPP2; UDPGP2; pHC379
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
CDC25C, Monoclonal Antibody (Cat# AAA24164)
Full Name
CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60) (AP)
Gene Names
CDC25C; CDC25; PPP1R60
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
RSK3, Monoclonal Antibody (Cat# AAA24347)
Full Name
RSK3, CT (Ribosomal S6 Kinase 3, RSK-3, pp90RSK3, 90kD Ribosomal Protein S6 Kinase 2, p90-RSK 2, p90RSK2, MAP Kinase-activated Protein Kinase 1c, MAPK-activated Protein Kinase 1c, MAPKAP Kinase 1c, MAPKAPK1C, MAPKAPK-1c, Ribosomal Protein S6 Kinase alpha-
Gene Names
RPS6KA2; RSK; HU-2; RSK3; p90RSK2; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Western BlotPositive WB detected in Recombinant proteinAll lanes: ftnA antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 24.8 kDaObserved band size: 30 kDa)
ftnA, Polyclonal Antibody (Cat# AAA27053)
Full Name
Rabbit anti-Helicobacter pylori (strain 700392/26695)(Campylobacter pylori) ftnA Polyclonal antibody
Reactivity
Helicobacter pylori
Applications
Western Blot
Purity
Protein A/G
Pricing
WB (Western Blot)
(Western blot analysis on V5-tagged recombinant protein (50kDa) using anti-V5 polyclonal antibody.)
V5-Tag, Polyclonal Antibody (Cat# AAA17983)
Full Name
V5-Tag Antibody
Applications
Western Blot
Purity
Immunogen affinity purified
Pricing
WB (Western Blot)
(Positive WB detected in Recombinant proteinAll lanes: gB antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 43.3 kDaObserved band size: 46 kDa)
gB, Polyclonal Antibody (Cat# AAA27058)
Full Name
Rabbit anti-Epstein-Barr virus (strain B95-8)(HHV-4)(Human herpesvirus 4) gB Polyclonal Antibody
Reactivity
Epstein-Barr virus
Applications
Western Blot
Purity
Protein G
Pricing
ENO3, Monoclonal Antibody (Cat# AAA24202)
Full Name
ENO3 (Enolase 3, 2-phospho-D-glycerate Hydro-lyase, beta-Enolase, Muscle-specific Enolase, MSE, Skeletal Muscle Enolase) (AP)
Gene Names
ENO3; MSE; GSD13
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
Application Data
(Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SOX10, Monoclonal Antibody (Cat# AAA13845)
Full Name
SOX10 (Melanoma Marker) Mouse Monoclonal Antibody
Gene Names
SOX10; DOM; WS4; PCWH; WS2E; WS4C
Reactivity
Human, Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
WB (Western Blot)
(Western Blot: The cell lysate of T293 with over-expressed recombinant protein GFP was resolved onto 10% SDS-PAGE, then transferred onto NC membrane. Followed by an immune-blotting with Rabbit anti- GFP (AAA14083) at 1:1000.)
GFP, Antibody (Cat# AAA14083)
Full Name
Rabbit anti GFP Polyclonal antibody
Reactivity
Tag Antibody
Applications
Western Blot, Immunoprecipitation
Purity
The Rabbit IgG is purified by Affinity Chromatography.
Pricing
LeY, Monoclonal Recombinant Antibody (Cat# AAA14186)
Full Name
Anti-LeY [H18A]
Reactivity
Human
Applications
Immunohistochemistry
Purity
Protein A affinity purified
Pricing
IF (Immunofluorescence)
(Fig.6. Immunofluorescence analysis of rat kidney tissue. 1, COX IV Monoclonal Antibody (14Y2) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
COX IV, Monoclonal Antibody (Cat# AAA31542)
Full Name
Anti-COX IV Mouse Monoclonal Antibody (14Y2)
Gene Names
COX4I1; COX4; COXIV; COX4-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Pricing
FCM (Flow Cytometry)
(FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)
STIP1, Monoclonal Antibody (Cat# AAA26408)
Full Name
STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (FITC)
Gene Names
STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Pricing
FCM (Flow Cytometry)
(FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)
STIP1, Monoclonal Antibody (Cat# AAA26275)
Full Name
STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (Biotin)
Gene Names
STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Pricing
WB (Western Blot)
(Western BlotPositive WB detected in: recombinant proteinAll lanes: psaA Antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42 kDaObserved band size: 42 kDa)
psaA, Polyclonal Antibody (Cat# AAA27055)
Full Name
psaA Antibody
Reactivity
Streptococcus pneumoniae
Applications
Western Blot
Purity
Affinity-chromatography
Pricing
IHC (Immunohistchemistry)
(Fig.6. Immunohistochemical analysis of paraffin-embedded rat kidney tissue. 1, alpha-tubulin Monoclonal Antibody (3G5) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
alpha-Tubulin, Monoclonal Antibody (Cat# AAA31544)
Full Name
Anti-alpha-Tubulin Monoclonal Antibody (3G5)
Gene Names
TUBA1A; LIS3; TUBA3; B-ALPHA-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Pricing
WB (Western Blot)
(Positive WB detected in Recombinant proteinAll lanes: BZLF1 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 46 kDaObserved band size: 50 kDa)
BZLF1, Polyclonal Antibody (Cat# AAA27059)
Full Name
Rabbit anti-Epstein-Barr virus (strain B95-8)(HHV-4)(Human herpesvirus 4) BZLF1 Polyclonal Antibody
Reactivity
Epstein-Barr virus
Applications
Western Blot
Purity
> 95%, Protein G purified
Pricing
IHC (Immunohistchemistry)
(Fig.6. Immunohistochemical analysis of paraffin-embedded rat lung tissue. 1, beta-Tubulin Monoclonal Antibody (3G6) was diluted at 1:400 (4°C, overnight). Negative control was used by secondary antibody only.)
beta-Tubulin, Monoclonal Antibody (Cat# AAA31540)
Full Name
Anti-beta-Tubulin Mouse Monoclonal Antibody (3G6)
Gene Names
TUBB3; CDCBM; TUBB4; beta-4; CFEOM3A
Reactivity
Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Sheep, Insect, Yeast, Hamster
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12081)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
Western Blot
Pricing
WB (Western Blot)
(STK33 monoclonal antibody, Western Blot analysis of STK33 expression in HeLa.)
STK33, Monoclonal Antibody (Cat# AAA25856)
Full Name
STK33 (Serine/Threonine Kinase 33) (PE)
Reactivity
Human
Applications
Immunofluorescence, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(Fig.8 Western blot analysis of Hela, GAPDH Monoclonal Antibody (2B5) was diluted at 1:10000 (25°C, 3h).)
GAPDH, Monoclonal Antibody (Cat# AAA31539)
Full Name
Anti-GAPDH Mouse Monoclonal Antibody (2B5)
Gene Names
GAPDH; G3PD; GAPD
Reactivity
Human, Mouse, Rat, Monkey, Dog, Chicken, Rabbit, Pig, Sheep, Yeast, Hamster
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Pricing
IF (Immunofluorescence)
(Fig.7. Immunofluorescence analysis of rat liver tissue. 1, Histone H3 Monoclonal Antibody (2D10) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 Labeled secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A: Target. Picture B: DAPI. Picture C: merge of A+B.)
Histone H3, Monoclonal Antibody (Cat# AAA31543)
Full Name
Anti-Histone H3 Mouse Monoclonal Antibody (2D10)
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat, Yeast
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
Pricing
IHC (Immunohistchemistry)
(Fig.6. Immunohistochemical analysis of paraffin-embedded rat testis tissue. 1, PCNA Monoclonal Antibod y(1D7) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C, 20min). 3, secondary antibody was diluted at 1:200 (room temperature, 30min). Negative control was used by secondary antibody only.)
PCNA, Monoclonal Antibody (Cat# AAA31541)
Full Name
Anti-PCNA Mouse Monoclonal Antibody (1D7)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11864)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
Immunofluorescence
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11930)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
WB (Western Blot)
(Western BlotPositive WB detected in: recombinant proteinAll lanes: csgA Antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 17 kDaObserved band size: 17 kDa)
csgA, Polyclonal Antibody (Cat# AAA27056)
Full Name
csgA Antibody
Gene Names
csgA; agfA; ECK1028; JW1025
Reactivity
E Coli (strain K12)
Applications
Western Blot
Purity
Affinity-chromatography
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA12211)
Full Name
MOUSE ANTI V5-TAG
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot, Radioimmunoassay
Pricing
WB (Western Blot)
(Positive WB detected in Recombinant proteinAll lanes: L1R antibody at 1:2000Secondary Goat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 26.8 kDabserved band size: 29 kDa)
L1R, Polyclonal Antibody (Cat# AAA27054)
Full Name
Rabbit anti-Vaccinia virus (strain Copenhagen)(VACV) L1R Polyclonal Antibody
Reactivity
Vaccinia virus
Applications
Western Blot
Purity
Protein G
Pricing
Application Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for the detection of V5 tagged WEEV_nsP3 protein by western blotting and immunofluorescenceImage caption: WEEV nsP3 interaction with host IKKbeta. A) U87MGs were transfected in a 6-well plate with 5 ug of pUC19 and WEEV_nsP3_HA for 24 hours. Cell lysates were resolved using SDS-PAGE and subsequently immunoblotted with V5 antibody and beta-actin served as a loading control. B) U87MGs were transfected with WEEV_nsP3_V5; cells were fixed after 24 hours and stained with antibodies against the endogenous IKKbeta and the V5 tag. Cells were incubated with appropriate secondary Alexa Fluor antibodies and the nuclei stained with DAPI. Co-localization of IKKbeta with WEEV_nsP3_V5 (yellow) was observed as shown by the arrows. B) Panels E -H serve as an example of transfected cells in a given field of view that show co-localization of IKKbeta and WEEV_nsP3_V5 24 hours post transfection. Panels I-L represent magnified images of other cells showing co-localization of IKKbeta and WEEV_nsP3_V5. Panel M is a magnified image of panel L. The co-localization was confirmed by Z-stack analysis. Co-localization was calculated to be approximately in 61% of cells (163 cells were counted of which 44% demonstrated expression of nsP3. Of those cells that expressed nsP3, 61% showed co-localization of both proteins). Images were taken using Nikon Eclipse TE2000-U at 60x magnification and are representative of 2 independent experiments.From: Amaya M, Voss K, Sampey G, Senina S, de la Fuente C, et al. (2014) The Role of IKKbeta in Venezuelan Equine Encephalitis Virus Infection. PLoS ONE 9(2): e86745.)
V5-TAG, Monoclonal Antibody (Cat# AAA11850)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
Immunohistochemistry, Western Blot
Pricing
IF (Immunofluorescence)
(Immunofluorescent staining using Western Blot analysis using Mouse anti Human IgG (Fc Specific) antibody (AAA14315) (Mouse anti Human IgG (Fc Specific) antibody))
Mouse anti Human IgG (Fc Specific) antibody, Monoclonal Antibody (Cat# AAA14315)
Full Name
Mouse anti Human IgG (Fc Specific) antibody
Applications
Immunofluorescence, Western Blot
Pricing
DUSP6, Monoclonal Antibody (Cat# AAA24194)
Full Name
DUSP6 (Dual Specificity Protein Phosphatase 6, Dual Specificity Protein Phosphatase PYST1, Mitogen-activated Protein Kinase Phosphatase 3, MAP Kinase Phosphatase 3, MKP3, MKP-3, PYST1) (AP)
Gene Names
DUSP6; HH19; MKP3; PYST1
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing
FCM (Flow Cytometry)
(FACS analysis of ES-2 cells stained with STIP1 monoclonal antibody clone 2E11 (Green) and non-stained ES-2 cells (Black) as negative control.)
STIP1, Monoclonal Antibody (Cat# AAA26142)
Full Name
STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (APC)
Gene Names
STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified
Pricing
WB (Western Blot)
(Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
MUTYH, Monoclonal Antibody (Cat# AAA24577)
Full Name
MUTYH (A/G-specific Adenine DNA Glycosylase, MutY Homolog, hMYH, MYH) APC
Gene Names
MUTYH; MYH
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(ADPGK monoclonal antibody. Western Blot analysis of ADPGK expression in Raw 264.7.)
ADPGK, Monoclonal Antibody (Cat# AAA25600)
Full Name
ADPGK (ADP-dependent Glucokinase, ADP-GK, RbBP-35, PSEC0260, 2610017G09Rik, DKFZp434B195) (PE)
Gene Names
ADPGK; ADP-GK; 2610017G09Rik
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
WB (Western Blot)
(CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in NIH/3T3.)
CDADC1, Monoclonal Antibody (Cat# AAA25345)
Full Name
CDADC1 (Cytidine and dCMP Deaminase Domain-containing Protein 1, Testis Development Protein NYD-SP15, MGC150615, MGC41774, MGC57136, NYD-SP15, BA103J18.1) (HRP)
Gene Names
CDADC1; NYD-SP15; bA103J18.1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
PKNOX2, Monoclonal Antibody (Cat# AAA24320)
Full Name
PKNOX2 (PREP2, Homeobox Protein PKNOX2, Homeobox Protein PREP-2, PBX/Knotted Homeobox 2, FLJ13074) (AP)
Gene Names
PKNOX2; PREP2
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
FCM (Flow Cytometry)
(Staining of C57BL/6 splenocytes with 0.25 ug of Mouse IgG2a kappa Isotype Control (FITC) (open histogram) or 0.25 ug of Qa-2 antibody (FITC) (filled histogram). Total viable cells were used for analysis.)
Qa2, Monoclonal Antibody (Cat# AAA17929)
Full Name
Qa2 antibody (FITC)
Gene Names
H2-Q7; Q9; Ped; Qa7; Qa-2; Qa-7; H-2Q7
Applications
Flow Cytometry
Pricing
PITX1, Monoclonal Antibody (Cat# AAA24319)
Full Name
PITX1 (Pituitary Homeobox 1, Paired-like Homeodomain Transcription Factor 1, Homeobox Protein PITX1, Hindlimb-expressed Homeobox Protein Backfoot, PTX1, BFT) (AP)
Gene Names
PITX1; BFT; CCF; POTX; PTX1; LBNBG
Reactivity
Human
Applications
EIA, IP, WB
Purity
Purified by Protein A Affinity Chromatography.
Pricing