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1056 results for "Conjugated Primary Antibody" - showing 500-550
FCM (Flow Cytometry)
(Flow cytometric analysis of HL-60 cells with Arp3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Arp3, Monoclonal Antibody (Cat# AAA30475)
Full Name
Arp3 Antibody
Gene Names
ACTR3; ARP3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with DIABLO antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
DIABLO, Monoclonal Antibody (Cat# AAA30141)
Full Name
DIABLO Antibody
Gene Names
DIABLO; SMAC; DFNA64
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of A431 cells with RanGAP1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
RanGAP1, Monoclonal Antibody (Cat# AAA30497)
Full Name
RanGAP1 Antibody
Gene Names
RANGAP1; SD; Fug1; KIAA1835; MGC20266; RANGAP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with Septin 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Septin2, Monoclonal Antibody (Cat# AAA30437)
Full Name
Septin 2 Antibody
Gene Names
SEPT2; DIFF6; NEDD5; Pnutl3; hNedd5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Argonaute 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Argonaute 2, Monoclonal Antibody (Cat# AAA30277)
Full Name
Argonaute 2 Antibody
Gene Names
AGO2; Q10; EIF2C2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of THP-1 cells with eEF1A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
EEF1A1, Monoclonal Antibody (Cat# AAA30467)
Full Name
EEF1A1 Antibody
Gene Names
EEF1A1; CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Vimentin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the second antibody)
Vimentin, Monoclonal Antibody (Cat# AAA30119)
Full Name
Vimentin Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry)
(AAA31132 at 1/100 staining rat testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
EFNB2, Polyclonal Antibody (Cat# AAA31132)
Full Name
EFNB2 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of A549 cells with PRPF8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
PRPF8, Monoclonal Antibody (Cat# AAA30485)
Full Name
PRPF8 Antibody
Gene Names
PRPF8; PRP8; RP13; HPRP8; PRPC8; SNRNP220
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of HeLa cells, using S100A4 Antibody.)
S100A4, Monoclonal Antibody (Cat# AAA30187)
Full Name
S100A4 Antibody
Gene Names
S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, IP, FC/FACS
Purity
Affinity-chromatography
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with HDAC8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
HDAC8, Monoclonal Antibody (Cat# AAA30223)
Full Name
HDAC8 Antibody
Gene Names
HDAC8; HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of A549 cells with UQCRFS1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
UQCRFS1, Monoclonal Antibody (Cat# AAA30490)
Full Name
UQCRFS1 Antibody
Gene Names
UQCRFS1; RIP1; RIS1; RISP; UQCR5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
ProA affinity purified
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12186)
Full Name
RAT ANTI MOUSE CD11b:RPE
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Flow Cytometry
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of 293 cells with RALBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
RALBP1, Monoclonal Antibody (Cat# AAA30149)
Full Name
RALBP1 Antibody
Gene Names
RALBP1; RIP1; RLIP1; RLIP76
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HUVEC cells with LYVE1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
LYVE1, Monoclonal Antibody (Cat# AAA30270)
Full Name
LYVE1 Antibody
Gene Names
LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with STAT5a antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
STAT5a, Monoclonal Antibody (Cat# AAA30129)
Full Name
STAT5a Antibody
Gene Names
STAT5A; MGF; STAT5
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry)
(IHC image of AAA27003 diluted at 1:200 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
DMD, Polyclonal Antibody (Cat# AAA27003)
Full Name
DMD Antibody
Gene Names
DMD; BMD; CMD3B; MRX85; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272
Reactivity
Human
Applications
Immunohistochemistry
Purity
>95%, Protein G purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with MEK3+MEK6 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
MEK3+MEK6, Monoclonal Antibody (Cat# AAA30231)
Full Name
MEK3+MEK6 Antibody
Gene Names
MAP2K3; MEK3; MKK3; MAPKK3; PRKMK3; SAPKK2; SAPKK-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
alpha 1 Catenin, Polyclonal Antibody (Cat# AAA31379)
Full Name
alpha 1 Catenin Antibody
Gene Names
CTNNA1; CAP102
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IP (Immunoprecipitation)
(EpCAM was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell LysateLane B:0.5 mg A431 Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell LysateLane D:0.5 mg Caco-2 Whole Cell Lysate0.5 uL anti-EpCAM rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-EpCAM rabbit monoclonal antibody,at 1:5000 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 40 kDaObserved band size: 40 kDa)
EpCAM, Monoclonal Antibody (Cat# AAA27736)
Full Name
Recombinant Anti-EpCAM Antibody, Rabbit Monoclonal
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Protein A
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Apg7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Apg7, Monoclonal Antibody (Cat# AAA30127)
Full Name
Apg7 Antibody
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of MCF-7 cells with PIST antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
PIST, Monoclonal Antibody (Cat# AAA30504)
Full Name
PIST Antibody
Gene Names
GOPC; CAL; FIG; PIST; GOPC1; dJ94G16.2
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human testis tissue reacted with PARK2 (Parkin) antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Parkin, Polyclonal Antibody (Cat# AAA28668)
Full Name
Parkin Antibody (C-term)
Gene Names
PARK2; PDJ; PRKN; AR-JP; LPRS2
Reactivity
Human, mouse
Applications
WB, EIA, IF, FC/FACS, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistchemistry)
(At 1/100 staining Human gastric cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
SRPK2, Polyclonal Antibody (Cat# AAA31293)
Full Name
Phospho-SRPK2 (Ser494/Ser497) Antibody
Gene Names
SRPK2; SFRSK2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with TDP43 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
TDP43, Monoclonal Antibody (Cat# AAA30332)
Full Name
TDP43 Antibody
Gene Names
TARDBP; ALS10; TDP-43
Reactivity
Human, Mouse, Rat, Zebrafish
Applications
Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with SHP1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
SHP1, Monoclonal Antibody (Cat# AAA29986)
Full Name
SHP1 Antibody
Gene Names
PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
IHC (Immunohistchemistry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
PACSIN2, Polyclonal Antibody (Cat# AAA28758)
Full Name
PACSIN2 Antibody (C-term)
Gene Names
PACSIN2; SDPII
Reactivity
Human, mouse
Applications
Immunohistochemistry, Western Blot, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with E2F1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
E2F1, Monoclonal Antibody (Cat# AAA30246)
Full Name
E2F1 Antibody
Gene Names
E2F1; RBP3; E2F-1; RBAP1; RBBP3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12231)
Full Name
RAT ANTI MOUSE CD11b:Low Endotoxin
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SiHa cells with IQGAP1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IQGAP1, Monoclonal Antibody (Cat# AAA30507)
Full Name
IQGAP1 Antibody
Gene Names
IQGAP1; SAR1; p195; HUMORFA01
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with Chk1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Chk1, Monoclonal Antibody (Cat# AAA30108)
Full Name
Chk1 Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of PC-3M cells with BAP31 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
BAP31, Monoclonal Antibody (Cat# AAA30501)
Full Name
BAP31 Antibody
Gene Names
BCAP31; CDM; BAP31; 6C6-AG; DXS1357E
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
Application Data
(Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)
CD11b, Monoclonal Antibody (Cat# AAA12181)
Full Name
RAT ANTI MOUSE CD11b
Gene Names
Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation
Pricing
Application Data
HIV-1 p24, Monoclonal Antibody (Cat# AAA13685)
Full Name
Anti-HIV-1 p24
Reactivity
Reacts with P24(HIC-1) proteins
Applications
Western Blot
Purity
Ion exchange column
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with ATF7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ATF7, Monoclonal Antibody (Cat# AAA30228)
Full Name
ATF7 Antibody
Gene Names
ATF7; ATFA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with TNFAIP3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
TNFAIP3, Monoclonal Antibody (Cat# AAA30161)
Full Name
TNFAIP3 Antibody
Gene Names
TNFAIP3; A20; OTUD7C; TNFA1P2
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
WB (Western Blot)
(WB Suggested Anti-SENP3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: 721_B cell lysateSENP3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)
SENP3, Polyclonal Antibody (Cat# AAA23562)
Full Name
SENP3 antibody - N-terminal region
Gene Names
SENP3; SSP3; Ulp1; SMT3IP1
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
Immunofluorescence, Western Blot
Purity
Affinity Purified
Pricing
Application Data
(Published clone specific image: Flow cytometric analysis of AM from NO2-exposed and control rats. Rats were exposed to NO2 for the indicated times and BAL cells were stained with antibodies to ED7, ED9, RM-4, and OX-6. To overcome autofluorescence signals, primary antibodies were detected using a biotin-PE/streptavidin-anti-streptavidin enhancing system and labeling of AM was analyzed by flow cytometry following gating by help of forward and sideward scatter properties. Shown are representative results of at least six animals per group.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)
CD172a, Monoclonal Antibody (Cat# AAA12001)
Full Name
MOUSE ANTI RAT CD172a
Gene Names
Sirpa; Bit; Ptpns1; SHPS-1
Applications
Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with MTCO2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
MTCO2, Monoclonal Antibody (Cat# AAA30139)
Full Name
MTCO2 Antibody
Gene Names
MT-CO2; COII; MTCO2; COX2
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY-5Y cells with RACK1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
RACK1, Monoclonal Antibody (Cat# AAA30521)
Full Name
RACK1 Antibody
Gene Names
GNB2L1; H12.3; HLC-7; PIG21; RACK1; Gnb2-rs1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Hela cells with RAD18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
RAD18, Monoclonal Antibody (Cat# AAA30157)
Full Name
RAD18 Antibody
Gene Names
RAD18; RNF73
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of A549 cells with FGFR3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
FGFR3, Monoclonal Antibody (Cat# AAA30329)
Full Name
FGFR3 Antibody
Gene Names
FGFR3; ACH; CEK2; JTK4; CD333; HSFGFR3EX
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of HepG2 cells with AAA27002 at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
SLC26A4, Polyclonal Antibody (Cat# AAA27002)
Full Name
SLC26A4 Antibody
Gene Names
SLC26A4; EVA; PDS; DFNB4; TDH2B
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%, Protein G purified
Pricing
Application Data
(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)
JAK3, Polyclonal Antibody (Cat# AAA31405)
Full Name
Phospho-JAK3 (Tyr981) Antibody
Gene Names
JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig (100%), Bovine (91%), Horse (100%), Sheep (100%), Dog (100%)
Predicted Reactivity: Pig (100%), Bovine (91%), Horse (100%), Sheep (100%), Dog (100%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data
(Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
CD206, Monoclonal Antibody (Cat# AAA12119)
Full Name
RAT ANTI MOUSE CD206:FITC
Gene Names
Mrc1; MR; CD206; AW259686
Applications
Flow Cytometry
Pricing
IF (Immunofluorescence)
(Immunofluorescence - anti-mScarlet Ab using hCEC cells transduced with mScarlet-Rab5a; cells were fixed with methanol and anti-mScarlet at 1/250;)
mScarlet, Polyclonal Antibody (Cat# AAA13885)
Full Name
anti-mScarlet
Reactivity
Reacts with Transfected cells proteins
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Immunoelectron Microscopy
Purity
This antibody is epitope-affinity purified from goat antiserum.
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY-5Y cells with CRMP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
CRMP1, Monoclonal Antibody (Cat# AAA30425)
Full Name
CRMP1 Antibody
Gene Names
CRMP1; DRP1; DRP-1; CRMP-1; DPYSL1; ULIP-3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of Jurkat cells with ZAP70 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ZAP70, Monoclonal Antibody (Cat# AAA30410)
Full Name
ZAP70 Antibody
Gene Names
ZAP70; SRK; STD; TZK; STCD; ZAP-70
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of K562 cells with Proteasome 20S C2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Proteasome 20S C2, Monoclonal Antibody (Cat# AAA30459)
Full Name
Proteasome 20S C2 Antibody
Gene Names
PSMA1; NU; HC2; PROS30; HEL-S-275
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS, IP
Purity
ProA affinity purified
Pricing
FCM (Flow Cytometry)
(Flow cytometric analysis of SH-SY-5Y cells with STUB1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
STUB1, Monoclonal Antibody (Cat# AAA30505)
Full Name
STUB1 Antibody
Gene Names
STUB1; CHIP; UBOX1; SCAR16; HSPABP2; NY-CO-7; SDCCAG7
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing