FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with MTCO2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Rabbit anti-Human MTCO2 Monoclonal Antibody | anti-MTCO2 antibody
MTCO2 Antibody
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with MTCO2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
FCM (Flow Cytometry)
(Flow cytometric analysis of HepG2 cells with MTCO2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry)
(ICC staining MTCO2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining MTCO2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining MTCO2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining MTCO2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining MTCO2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining MTCO2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MTCO2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MTCO2 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of MTCO2 on different lysates using anti-MTCO2 antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: THP-1 Lane 3: Hela)
WB (Western Blot)
(Western blot analysis of MTCO2 on different lysates using anti-MTCO2 antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: THP-1 Lane 3: Hela)
NCBI and Uniprot Product Information
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Product Notes
The MTCO2 mt-co2 (Catalog #AAA30139) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MTCO2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MTCO2 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IP (Immunoprecipitation), FCM/FACS (Flow Cytometry). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the MTCO2 mt-co2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MTCO2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is the new monoclonal antibody for COVID?
MTCO2 is not a COVID antibody; it targets mitochondrial cytochrome c oxidase subunit II (MTCO2), a mitochondrial inner membrane protein involved in cellular respiration. COVID monoclonal antibodies target SARS-CoV-2 spike or nucleocapsid proteins.
Which species does this MTCO2 antibody detect?
The MTCO2 antibody detects human, mouse, and rat MTCO2 protein. It shows cross-reactivity across these mammalian species and is commonly validated in human cell lines like HeLa.
What is the clone number and isotype of the antibody?
MTCO2 antibodies vary by manufacturer; commonly available versions include polyclonal rabbit anti-MTCO2 IgG. Specific clone numbers depend on the vendor; check individual product datasheets for monoclonal clone identifiers and isotype information.
What dilution is recommended for mitochondrial staining or protein detection?
Recommended dilutions are 1:50–1:200 for immunohistochemistry (IHC) and immunofluorescence (IF/ICC), and 1:1,000–1:5,000 for Western blotting. Optimize dilutions based on cell type, detection method, and signal intensity requirements.
What storage conditions are required to maintain antibody stability?
Store at 2–8°C for short-term use or –20°C for long-term storage, protecting from light. Avoid repeated freeze-thaw cycles. For extended stability, lyophilized formats can be stored at –20°C for 3–5 years without loss of activity.
Does this antibody specifically detect mitochondrial MTCO2?
Yes, the MTCO2 monoclonal antibody (Clone sc06-88) specifically recognizes mitochondrial MTCO2 (cytochrome c oxidase subunit II), a key component of the electron transport chain. This antibody demonstrates high specificity for the target protein, making it suitable for precise detection in various applications.
Which species does the MTCO2 antibody react with?
The MTCO2 antibody reacts with human samples and has been validated for use in Western blotting and other immunological applications. Reactivity with other mammalian species may vary; AAA Biotech product documentation should be consulted for cross-reactivity information with specific non-human species.
What fixation method works best for MTCO2 staining?
For immunocytochemistry and immunohistochemistry applications, standard fixation protocols using 4% paraformaldehyde or ethanol-based fixatives are generally compatible with MTCO2 antibodies. Specific fixation recommendations depend on the intended application and specimen type; consultation with AAA Biotech technical support is recommended for optimized staining protocols.
How should the antibody be stored to maintain stability?
The MTCO2 antibody should be stored at 4°C (refrigerated) in a buffered solution, typically containing glycerol and minimal preservative (such as <0.02% sodium azide). Do not freeze or undergo freeze-thaw cycles, as this can compromise antibody functionality. Store in the original container with minimal disturbance for maximum stability.
What is MTCO2 stand for?
MTCO2 stands for Mitochondrially Encoded Cytochrome c Oxidase Subunit II. It is a protein found inside mitochondria and is part of the enzyme complex that helps cells produce energy. Scientists study it to understand how mitochondria work.
Can MTCO2 antibody be used to visualize mitochondria in cells?
Yes, the MTCO2 antibody can be used to visualize mitochondria in cells. MTCO2 lives inside mitochondria; staining it allows researchers to see its structure and distribution using microscope-based methods like immunofluorescence.
Is MTCO2 expression an indicator of mitochondrial activity or health?
Yes, MTCO2 expression reflects mitochondrial activity and health. It's a part of the energy-producing system. So, changes in its levels suggest problems or changes in mitochondrial function, especially in stressed cells.
Can this antibody be used for immunofluorescence or Western blot?
Yes, this antibody can be used for immunofluorescence or Western blot. These methods help researchers detect where MTCO2 is located in cells and measure how much of the protein is present in samples.
How does MTCO2 differ from other mitochondrial markers like TOM20?
MTCO2 is part of the mitochondrial inner membrane and is involved in energy production. Whereas TOM20 is located on the outer mitochondrial membrane and helps import proteins.
Is MTCO2 commonly studied in metabolic or neurodegenerative research?
Yes, MTCO2 is commonly studied in metabolic and neurodegenerative research. Mitochondria are key to energy production, and changes in MTCO2 are linked to disorders like Alzheimer’s, Parkinson’s, and metabolic diseases, where energy balance is affected.
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