Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
898 results for "HRP Conjugated Secondary Antibody" - showing 450-500
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of U87 cells using anti-GNG2 antibody (AAA19312).Overlay histogram showing U87 cells stained with AAA19312 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GNG2 Antibody (AAA19312, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
GNG2, Polyclonal Antibody (Cat# AAA19312)
Full Name
Anti-GNG2 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of 293T cells using anti-CDC45L antibody (AAA19360).Overlay histogram showing 293T cells stained with AAA19360 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- CDC45L Antibody (AAA19360, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CDC45L, Monoclonal Antibody (Cat# AAA19360)
Full Name
Anti-CDC45L Antibody (monoclonal, 6H6)
Gene Names
CDC45; CDC45L; CDC45L2; PORC-PI-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Figure 1. Western blot analysis of CISD2 using anti-CISD2 antibody (AAA19311).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human HELA whole cell lysatesLane 3: human MCF-7 whole cell lysatesLane 4: monkey kidney tissue lysatesLane 5: human SW620 whole cell lysatesLane 6: human Raji whole cell lysatesLane 7: rat kidney tissue lysatesLane 8: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CISD2 antigen affinity purified polyclonal antibody (Catalog # AAA19311) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for CISD2 at approximately 15KD. The expected band size for CISD2 is at 15KD.)
CISD2, Polyclonal Antibody (Cat# AAA19311)
Full Name
Anti-CISD2 Antibody
Gene Names
CISD2; ERIS; WFS2; ZCD2; NAF-1; Miner1
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of RH35 cells using anti- EIF4A1 antibody (AAA19380).Overlay histogram showing RH35 cells stained with AAA19380 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-EIF4A1 Antibody (AAA19380, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
EIF4A1, Monoclonal Antibody (Cat# AAA19380)
Full Name
Anti-EIF4A1 Antibody (monoclonal, 11B8)
Gene Names
EIF4A1; DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 13. Flow Cytometry analysis of MCF-7 cells using anti-EHD3 antibody (AAA19295).Overlay histogram showing MCF-7 cells stained with AAA19295 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EHD3 Antibody (AAA19295, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
EHD3, Polyclonal Antibody (Cat# AAA19295)
Full Name
Anti-EHD3 Antibody
Gene Names
EHD2; PAST2
Reactivity
Human, Monkey, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(FG Pancreatic Carcinoma Cell Lines stably expressing vector along (FG-V) the b3 integrin subunit (FG-b3) or a b3 truncation mutant (FG-759x). Src Mab (AAA28639) was diluted 1:500 in 1% BSA/TBST and incubated Overnight at 4 degree C. After washing 3x 5 min. with TBST the blots were incubated with 1:5000 Goat anti-mouse or Goat anti-rabbit secondary antibody for 1 hr at Room temperature. The blots were again washed 3x 5 min. with TBST and developed using ECL reagent.Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.)
SRC, Monoclonal Antibody (Cat# AAA28639)
Full Name
SRC Antibody
Gene Names
SRC; ASV; SRC1; c-SRC; p60-Src
Reactivity
Human, mouse
Applications
WB, EIA, IF
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
IF (Immunofluorescence)
(Figure 7. IF analysis of METTL1 using anti- METTL1 antibody (AAA19336).METTL1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-METTL1 Antibody (AAA19336) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
METTL1, Polyclonal Antibody (Cat# AAA19336)
Full Name
Anti-METTL1 Antibody
Gene Names
METTL1; TRM8; TRMT8; C12orf1; YDL201w
Reactivity
Human, Rat
Applications
WB, IHC-P, ICC, IF, EIA
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Host: RabbitTarget Name: MUC1Sample Type: HepG2Lane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5ug/mlLysate Quantity: 25ug/lane/laneGel Concentration: 0.12)
MUC1, Polyclonal Antibody (Cat# AAA23492)
Full Name
MUC1 antibody - C-terminal region
Gene Names
MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Pig
Applications
IHC, WB
Purity
Affinity Purified
Pricing
DB (Dot Blot)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Phospho-HIST1H3B3 (S10), Polyclonal Antibody (Cat# AAA28670)
Full Name
Phospho-HIST1H3B3 (S10) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human
Applications
DB, EIA, IHC, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(WB Suggested Anti-SHH Antibody Titration: 0.2-1 ug/mlPositive Control: HepG2 cell lysate)
SHH, Polyclonal Antibody (Cat# AAA23516)
Full Name
SHH antibody - N-terminal region
Gene Names
SHH; TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5
Reactivity
Predicted Reactivity: Cow, Dog, Goat, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish, Chicken (Tested Reactivity: Human, Mouse, Chicken)
Applications
IHC, WB
Purity
Affinity Purified
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of U937 cells using anti-Coronin 1a/TACO/CORO1A antibody (AAA19291).Overlay histogram showing U937 cells stained with AAA19291 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Coronin 1a/TACO/CORO1A Antibody (AAA19291, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Coronin 1a/TACO/CORO1A, Polyclonal Antibody (Cat# AAA19291)
Full Name
Anti-Coronin 1a/TACO/CORO1A Antibody
Gene Names
CORO1A; p57; TACO; CLABP; HCORO1; CLIPINA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of A431 cells using anti-TMPRSS3 antibody (AAA19289).Overlay histogram showing A431 cells stained with AAA19289 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TMPRSS3 Antibody (AAA19289, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TMPRSS3, Polyclonal Antibody (Cat# AAA19289)
Full Name
Anti-TMPRSS3 Antibody
Gene Names
TMPRSS3; DFNB8; DFNB10; ECHOS1; TADG12
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of 293T cells using anti-TBP-1/PSMC3 antibody (AAA19314).Overlay histogram showing 293T cells stained with AAA19314 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TBP-1/PSMC3 Antibody (AAA19314, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
TBP-1/PSMC3, Polyclonal Antibody (Cat# AAA19314)
Full Name
Anti-TBP-1/PSMC3 Antibody
Gene Names
PSMC3; TBP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of U937 cells using anti-BDH1 antibody (AAA19328).Overlay histogram showing U937 cells stained with AAA19328 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BDH1 Antibody (AAA19328,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
BDH1, Polyclonal Antibody (Cat# AAA19328)
Full Name
Anti-BDH1 Antibody
Gene Names
BDH1; BDH; SDR9C1
Reactivity
Human, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 10. Flow Cytometry analysis of A549 cells using anti- U2AF65/U2AF2 antibody (AAA19375).Overlay histogram showing A549 cells stained with AAA19375 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-U2AF65/U2AF2 Antibody (AAA19375, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
U2AF65/U2AF2, Monoclonal Antibody (Cat# AAA19375)
Full Name
Anti-U2AF65/U2AF2 Antibody (monoclonal, 10F4)
Gene Names
U2AF2; U2AF65
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of HELA cells using anti-DNAJC10 antibody (AAA19320).Overlay histogram showing HELA cells stained with AAA19320 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DNAJC10 Antibody (AAA19320, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
DNAJC10, Polyclonal Antibody (Cat# AAA19320)
Full Name
Anti-DNAJC10 Antibody
Gene Names
DNAJC10; JPDI; MTHr; ERdj5; PDIA19
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of K562 cells using anti-D2AP antibody (M01756). Overlay histogram showing K562 cells stained with M01756 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-D2AP Antibody (M01756, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
CD2AP, Monoclonal Antibody (Cat# AAA19185)
Full Name
Anti-CD2AP Antibody (monoclonal, 5F8)
Gene Names
CD2AP; CMS
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of A549 cells using anti-Transketolase/TKT antibody (AAA19368).Overlay histogram showing A549 cells stained with AAA19368 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Transketolase/TKT Antibody (AAA19368, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Transketolase/TKT, Monoclonal Antibody (Cat# AAA19368)
Full Name
Anti-Transketolase/TKT Antibody (monoclonal, 2I3)
Gene Names
TKT; TK; TKT1; HEL107
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 11. Flow Cytometry analysis of RH35 cells using anti- eRF1/ETF1 antibody (AAA19382).Overlay histogram showing RH35 cells stained with AAA19382 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-eRF1/ETF1 Antibody (AAA19382, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
eRF1/ETF1, Monoclonal Antibody (Cat# AAA19382)
Full Name
Anti-eRF1/ETF1 Antibody (monoclonal, 3B6)
Gene Names
ETF1; ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of THP-1 cells using anti-INPPL1 antibody (AAA19364).Overlay histogram showing THP-1 cells stained with AAA19364 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- INPPL1 Antibody (AAA19364, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
INPPL1, Monoclonal Antibody (Cat# AAA19364)
Full Name
Anti-INPPL1 Antibody (monoclonal, 8C13)
Gene Names
INPPL1; OPSMD; SHIP2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of CD9 Antibody (Center) in HepG2 cell line lysates (35ug/lane). CD9 (arrow) was detected using the purified Pab.)
CD9, Polyclonal Antibody (Cat# AAA28656)
Full Name
CD9 Antibody (Center)
Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Reactivity
Human
Applications
WB, EIA, IF, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of U251 cells using anti-RAB1B antibody (AAA19296).Overlay histogram showing U251 cells stained with AAA19296 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB1B Antibody (AAA19296, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
RAB1B, Polyclonal Antibody (Cat# AAA19296)
Full Name
Anti-RAB1B Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
WB (Western Blot)
(Western blot analysis of lysates from 293, Hela, mouse NIH/3T3, rat PC-12 cell line and rat brain tissue lysate(from left to right), using RPS6 Antibody (N-term). AAA28643 was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
RPS6, Monoclonal Antibody (Cat# AAA28643)
Full Name
RPS6 Antibody (N-term)
Gene Names
RPS6; S6
Reactivity
Human, rat (Predicted Reactivity: Monkey, Mouse)
Applications
EIA, IHC, WB, FC/FACS, IF
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of MCF-7 cells using anti-RGS6 antibody (AAA19308).Overlay histogram showing MCF-7 cells stained with AAA19308 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RGS6 Antibody (AAA19308, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
RGS6, Polyclonal Antibody (Cat# AAA19308)
Full Name
Anti-RGS6 Antibody
Gene Names
RGS6; GAP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of THP-1 cells using anti-PRMT8 antibody (AAA19321).Overlay histogram showing THP-1 cells stained with AAA19321 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PRMT8 Antibody (AAA19321,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PRMT8, Polyclonal Antibody (Cat# AAA19321)
Full Name
Anti-PRMT8 Antibody
Gene Names
PRMT8; HRMT1L3; HRMT1L4
Reactivity
Human, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 12. Flow Cytometry analysis of U937 cells using anti-DYNLL1/PIN antibody (AAA19276).Overlay histogram showing U937 cells stained with AAA19276 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DYNLL1/PIN Antibody (AAA19276, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
DYNLL1/PIN, Polyclonal Antibody (Cat# AAA19276)
Full Name
Anti-DYNLL1/PIN Antibody
Gene Names
DYNLL1; LC8; PIN; DLC1; DLC8; LC8a; DNCL1; hdlc1; DNCLC1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of A549 cells using anti-Hsp90 alpha antibody (AAA19359).Overlay histogram showing A549 cells stained with AAA19359 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Hsp90 alpha Antibody (AAA19359, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Hsp90 alpha, Monoclonal Antibody (Cat# AAA19359)
Full Name
Anti-Hsp90 alpha Antibody (monoclonal, 6B5)
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; HSP89A; HSP90A; HSP90N; HSPCAL1; HSPCAL4
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical of paraffin-embedded human esophageal tissue using AAA18801 at dilution of 1:200)
Cyfra21-1, Monoclonal Antibody (Cat# AAA18801)
Full Name
Mouse anti- human Cyfra21-1(KS19.1)monoclonal Antibody
Reactivity
Human
Applications
EIA, WB, IHC
Purity
>95%, Protein G Purified
Pricing
FCM (Flow Cytometry)
(Figure 7. Flow Cytometry analysis of U20S cells using anti- ADA antibody (AAA19184). Overlay histogram showing U20S cells stained with AAA19184 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- ADA Antibody (AAA19184, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
ADA, Monoclonal Antibody (Cat# AAA19184)
Full Name
Anti-ADA Antibody (monoclonal, 6D4)
Reactivity
Human
Applications
WB, IHC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
IF (Immunofluorescence)
(Figure 11. IF analysis of Tubulin alpha using anti- Tubulin alpha antibody (AAA19381).Tubulin alpha was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- Tubulin alpha Antibody (AAA19381) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Tubulin alpha, Monoclonal Antibody (Cat# AAA19381)
Full Name
Anti-Tubulin alpha Antibody (monoclonal, 7B12)
Gene Names
TUBA1A; LIS3; TUBA3; B-ALPHA-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry)
(PINK1 Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PINK1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)
PINK1, Monoclonal Antibody (Cat# AAA28638)
Full Name
PINK1 Antibody
Gene Names
PINK1; BRPK; PARK6
Reactivity
Human
Applications
EIA, IHC, IF, WB
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
FCM (Flow Cytometry)
(Figure 8. Flow Cytometry analysis of K562 cells using anti-PDIA6 antibody (AAA19282).Overlay histogram showing K562 cells stained with AAA19282 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDIA6 Antibody (AAA19282, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
PDIA6, Polyclonal Antibody (Cat# AAA19282)
Full Name
Anti-PDIA6 Antibody
Gene Names
PDIA6; P5; ERP5; TXNDC7
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry)
(Figure 9. Flow Cytometry analysis of K562 cells using anti-MCM6 antibody (AAA19372).Overlay histogram showing K562 cells stained with AAA19372 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- MCM6 Antibody (AAA19372, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
MCM6, Monoclonal Antibody (Cat# AAA19372)
Full Name
Anti-MCM6 Antibody (monoclonal, 10I9)
Gene Names
MCM6; Mis5; P105MCM; MCG40308
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
anti-Rabbit IgG+HRP, Secondary Antibody (Cat# AAA13854)
Full Name
anti-Rabbit IgG+HRP
Reactivity
Rabbit
Applications
Western Blot, Immunohistochemistry
Purity
Goats were immunized with highly purified Rabbit IgG
Affinity purified and conjugated with horseradish peroxidase (HRP)
Affinity purified and conjugated with horseradish peroxidase (HRP)
Pricing
WB (Western Blot)
(Fig1: Western blot analysis of Annexin A1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control:Lane 1: A431 cell lysateLane 2: Rat uterus tissue lysate)
Annexin A1 Autoantibody (Anti-ANXA1), ELISA Kit (Cat# AAA10593)
Full Name
Human Annexin A1 Autoantibody (Anti-ANXA1) ELISA Kit
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Pricing
IF (Immunofluorescence)
(AAA31072 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
LKB1, Polyclonal Antibody (Cat# AAA31072)
Full Name
Phospho-LKB1 (Ser428) Antibody
Gene Names
STK11; PJS; LKB1; hLKB1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(Western blot analysis of GPR84 expression in A431 whole cell lysates)
GPR84, Polyclonal Antibody (Cat# AAA31105)
Full Name
GPR84 Antibody
Gene Names
GPR84; EX33; GPCR4
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31248 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31248 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PDE1C, Polyclonal Antibody (Cat# AAA31248)
Full Name
PDE1C Antibody
Gene Names
PDE1C; Hcam3; DFNA74; hCam-3; cam-PDE 1C
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(88%), Horse(100%), Rabbit(88%), Dog(100%)
Predicted Reactivity: Pig(88%), Horse(100%), Rabbit(88%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(Immunofluorescent analysis of Ephrin A5 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Ephrin A5, Polyclonal Antibody (Cat# AAA27798)
Full Name
Anti-Ephrin A5 Antibody
Gene Names
EFNA5; AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Rabbit
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Flow Cytometry
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
WB (Western Blot)
(Western BlotPositive WB detected in: U87 whole cell lysateAll lanes: ATAT1 antibody at 1:1000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 45, 46, 47, 38, 37, 36, 34 kDaObserved band size: 47 kDa)
ATAT1, Polyclonal Antibody (Cat# AAA27001)
Full Name
ATAT1 Antibody
Gene Names
ATAT1; TAT; MEC17; C6orf134; Nbla00487; alpha-TAT; alpha-TAT1
Reactivity
Human
Applications
Western Blot
Purity
>95%, Protein G purified
Pricing
IF (Immunofluorescence)
(AAA31138 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(AAA31138) and mouse anti-beta tubulin Ab for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)
Prestin, Polyclonal Antibody (Cat# AAA31138)
Full Name
Prestin Antibody
Gene Names
SLC26A5; PRES; DFNB61
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(AAA31214 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31214 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
MUM1, Polyclonal Antibody (Cat# AAA31214)
Full Name
MUM1 Antibody
Gene Names
PWWP3A; MUM1; MUM-1; EXPAND1; HSPC211
Reactivity
Human, Mouse, Rat, Monkey
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(88%), Sheep(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(88%), Sheep(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31182 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
PU.1/Spi1, Polyclonal Antibody (Cat# AAA31182)
Full Name
PU.1/Spi1 Antibody
Gene Names
SPI1; OF; PU.1; SFPI1; SPI-1; SPI-A
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IF (Immunofluorescence)
(Figure 3 and 4: ICC/IF analysis of MIF in Balb/3T3 cells. The cell was stained with AAA11724 (1;100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucelus (blue).Figure 5 and 6: ICC/IF analysis of MIF in HeLa cells line. The cell was stained with AAA11724 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue))
MIF, Monoclonal Antibody (Cat# AAA11724)
Full Name
MIF antibody
Gene Names
MIF; GIF; GLIF; MMIF
Reactivity
Human
Applications
Western Blot, Immunocytochemistry, Immunofluorescence
Purity
By protein-G affinity chromatography
Pricing
IF (Immunoflouorescence)
(AAA31039 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
ADD1, Polyclonal Antibody (Cat# AAA31039)
Full Name
Phospho-ADD1 (Ser726) Antibody
Gene Names
ADD1; ADDA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA31175 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31175 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Nicotinic Acetylcholine Receptor alpha 7, Polyclonal Antibody (Cat# AAA31175)
Full Name
Nicotinic Acetylcholine Receptor alpha 7 Antibody
Gene Names
CHRNA7; NACHRA7; CHRNA7-2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AAA10763) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.)
IgG (H+L), Secondary Antibody (Cat# AAA10763)
Full Name
HRP Goat Anti-Rabbit IgG (H+L) Antibody
Reactivity
Rabbit
Applications
Western Blot, Immunohistochemistry, Dot Blot
Purity
Affinity Purification
Pricing
IF (Immunofluorescence)
(AAA31187 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31187 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Syntrophin alpha 1, Polyclonal Antibody (Cat# AAA31187)
Full Name
Syntrophin alpha 1 Antibody
Gene Names
SNTA1; SNT1; LQT12; TACIP1; dJ1187J4.5
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Rabbit(88%), Dog(88%)
Predicted Reactivity: Rabbit(88%), Dog(88%)
Applications
Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(AAA30993 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IGF1R, Polyclonal Antibody (Cat# AAA30993)
Full Name
Phospho-IGF1R (Tyr1346) Antibody
Gene Names
IGF1R; IGFR; CD221; IGFIR; JTK13
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry-Paraffin)
(AAA31263 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
UBR4, Polyclonal Antibody (Cat# AAA31263)
Full Name
UBR4 Antibody
Gene Names
UBR4; p600; ZUBR1; RBAF600
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing