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Reactivity
Gene Name
Isotype
Host
Application
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494 results for "Blocking Peptide" - showing 450-494
Application Data
(At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
PAR4, Polyclonal Antibody (Cat# AAA31375)
Full Name
Phospho-PAR4 (Thr163) Antibody
Gene Names
PAWR; PAR4; Par-4
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (92%), Rabbit (100%), Chicken (100%), Xenopus (92%)
Predicted Reactivity: Pig (100%), Zebrafish (92%), Rabbit (100%), Chicken (100%), Xenopus (92%)
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
ABCB10, Blocking Peptide (Cat# AAA13682)
Full Name
ABCB10 Immunizing Peptide
Gene Names
ABCB10; M-ABC2; MTABC2; EST20237
Reactivity
Human, Mouse, Rat
Pricing
WB (Western Blot)
(Validation Data Western blot analysis of extracts of various cell lines, using GAA antibody (AAA28197) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 60s)
GAA, Polyclonal Antibody (Cat# AAA28197)
Full Name
GAA Polyclonal Antibody
Gene Names
GAA; LYAG
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunoprecipitation
Purity
Affinity Purification
Pricing
IHC (Immunohistochemistry)
p16, Polyclonal Antibody (Cat# AAA13850)
Full Name
p16 (C20) Antibody
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Pricing
ChIP (Chromatin Immunoprecipitation)
(Chromatin immunoprecipitation was performed with cross-linked chromatin from 293T, using Acetyl-Histone H3-K27 Rabbit pAb antibody (AAA28163) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram constructed the ratios of the ratio of the immunoprecipitated DNA versus the input.)
H3K27AC, Antibody (Cat# AAA28163)
Full Name
Histone H3K27AC Polyclonal Antibody
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation
Purity
Affinity Purification
Pricing
WB (Western Blot)
(Western blot analysis of extracts of rat thymus, using T2R38 antibody (AAA27928) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 90s.)
T2R38, Polyclonal Antibody (Cat# AAA27928)
Full Name
Taste receptor type 2 member 38 (T2R38) (PTC bitter taste receptor) (Taste receptor type 2 member 61)
Gene Names
TAS2R38; PTC; T2R38; T2R61
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
WB (Western Blot)
(Anti-SLC6A19 Antibody (C-Term) at 1:2000 dilution + 293 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 71 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
SLC6A19, Polyclonal Antibody (Cat# AAA28802)
Full Name
SLC6A19 Antibody (C-Term)
Gene Names
SLC6A19; HND; B0AT1
Reactivity
Human
Applications
Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IF (Immunofluorescence)
(AAA31211 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA31211 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
SEPT4, Polyclonal Antibody (Cat# AAA31211)
Full Name
SEPT4 Antibody
Gene Names
SEPTIN4; H5; ARTS; MART; SEP4; CE5B3; SEPT4; PNUTL2; hucep-7; BRADEION; C17orf47; hCDCREL-2
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Predicted Reactivity: Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Pricing
IHC (Immunohistochemistry)
(AAA30939 at 1/100 staining rat appendiceal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Chk1, Polyclonal Antibody (Cat# AAA30939)
Full Name
Phospho-Chk1 (Ser280) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA31015 at 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
CREB, Polyclonal Antibody (Cat# AAA31015)
Full Name
Phospho-CREB (Ser142) Antibody
Gene Names
CREB1; CREB; CREB-1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immmunohistochemistry)
(PLA2G7 Antibody (Center) (Cat. #AAA28698)IHC analysis in formalin fixed and paraffin embedded tonsil followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PLA2G7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
PLA2G7, Polyclonal Antibody (Cat# AAA28698)
Full Name
PLA2G7 Antibody (Center)
Gene Names
PLA2G7; PAFAD; PAFAH; LP-PLA2; LDL-PLA2
Reactivity
Human
Applications
Immunohistochemistry, Flow Cytometry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(AAA30967 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Lamin A/C, Polyclonal Antibody (Cat# AAA30967)
Full Name
Phospho-Lamin A/C (Ser392) Antibody
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(AAA30924 staining MCF-7 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Ki67, Polyclonal Antibody (Cat# AAA30924)
Full Name
Ki67 Antibody
Gene Names
MKI67; KIA; MIB-; MIB-1; PPP1R105
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientifiv).
Pricing
IHC (Immunohistochemistry)
(At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Histone H3, Polyclonal Antibody (Cat# AAA31353)
Full Name
Acetyl-Histone H3 (Lys122) Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Bovine (100%)
Predicted Reactivity: Bovine (100%)
Applications
Western Blot, Immunohistochemistry, Peptide ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
IF (Immunofluorescence)
(Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with TNF using NFkappaB-p65 (Phospho-Ser276) Antibody.)
NFkappaB-p65, Polyclonal Antibody (Cat# AAA29703)
Full Name
NFkappaB-p65(Phospho-Ser276) Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of MMP12 Antibody (C-term) with 293 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)
MMP12, Polyclonal Antibody (Cat# AAA28713)
Full Name
MMP12 Antibody (C-term)
Gene Names
MMP12; ME; HME; MME; MMP-12
Reactivity
Human, mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(AAA30991 at 1/200 staining Rat brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary Ab.)
SP1, Polyclonal Antibody (Cat# AAA30991)
Full Name
Phospho-SP1 (Thr453) Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(All lanes : Anti-LAG3 Antibody (Center) at 1:1000 dilutionLane 1: human ovary lysateLane 2: mouse liver lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 57 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
LAG3, Polyclonal Antibody (Cat# AAA28766)
Full Name
LAG3 Antibody (Center)
Gene Names
LAG3; CD223
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
WB (Western Blot)
(Anti-Zebrafish hey2 Antibody (C-term)at 1:2000 dilution + ZF4 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
hey2, Polyclonal Antibody (Cat# AAA28798)
Full Name
Zebrafish hey2 Antibody (C-term)
Gene Names
hey2; grl; zgc:136746
Reactivity
Zebrafish
Applications
Western Blot
Pricing
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PI3K P85 a/y/beta antibody (left) or the same antibody preincubated with with blocking peptide (right))
PI3K, Polyclonal Antibody (Cat# AAA17933)
Full Name
PI3K antibody
Applications
Immunohistochemistry
Purity
PI3K antibody was purified by immunoaffinity chromatography
Pricing
WB (Western Blot)
(DRD2 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the DRD2 antibody detected the DRD2 protein (arrow).)
DRD2, Polyclonal Antibody (Cat# AAA28691)
Full Name
DRD2 Antibody (C-term)
Gene Names
DRD2; D2R; D2DR
Reactivity
Human, mouse
Applications
Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(SLC5A12 Antibody (C-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SLC5A12, Polyclonal Antibody (Cat# AAA28799)
Full Name
SLC5A12 Antibody (C-term)
Gene Names
SLC5A12; SMCT2
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Flow Cytometry
Pricing
WB (Western Blot)
(Western blot analysis of Phospho-Tyrosine Hydroxylase (Ser19) expression in various lysates)
Tyrosine Hydroxylase, Polyclonal Antibody (Cat# AAA30988)
Full Name
Phospho-Tyrosine Hydroxylase (Ser19) Antibody
Gene Names
TH; TYH; DYT14; DYT5b
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(All lanes : Anti-hDCAMKL1-V705 at 1:8000 dilution Lane 1: Human brain lysate Lane 2: Mouse brain lysate Lane 3: Rat brain lysate Lane 4: TT whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 82 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
DCAMKL1, Polyclonal Antibody (Cat# AAA28779)
Full Name
DCAMKL1 Antibody (C-term)
Gene Names
DCLK1; CL1; DCLK; CLICK1; DCDC3A; DCAMKL1
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IHC (Immunohistochemistry)
(AAA30944 at 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
HSP20, Polyclonal Antibody (Cat# AAA30944)
Full Name
Phospho-HSP20 (Ser16) Antibody
Gene Names
HSPB6; HEL55; Hsp20; PPP1R91
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry)
(KRAS Antibody (C-term)(AAA28748) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
KRAS, Polyclonal Antibody (Cat# AAA28748)
Full Name
KRAS Antibody (C-term)
Gene Names
KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
Reactivity
Human, Mouse, Rat (Predicted: Rat)
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistchemistry)
(Phospho-ERK1/2 (Thr202/Tyr204) Antibody for IHC in human testis)
ERK1/2, Polyclonal Antibody (Cat# AAA30930)
Full Name
Phospho-ERK1/2 (Thr202/Tyr204) Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IF (Immunofluorescence)
(LGR5/GPR49 Antibody (loop2) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).)
LGR5/GPR49, Polyclonal Antibody (Cat# AAA28712)
Full Name
LGR5/GPR49 Antibody (loop2)
Gene Names
LGR5; FEX; HG38; GPR49; GPR67; GRP49
Reactivity
Human, mouse, rat
Applications
Western Blot, Immunofluorescence, Flow Cytometry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
IF (Immunofluorescence)
(AAA30940 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
GSK3 beta, Polyclonal Antibody (Cat# AAA30940)
Full Name
Phospho-GSK3 beta (Ser9) Antibody
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation
Purity
Peptide affinity purification
Pricing
IHC (Immunohistochemistry)
(AAA30946 at 1/100 staining rat cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Chk1, Polyclonal Antibody (Cat# AAA30946)
Full Name
Phospho-Chk1 (Ser286) Antibody
Gene Names
CHEK1; CHK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistchemistry)
(GLG1 Antibody (C-term) (AAA28703) IHCanalysis in formalin fixed and paraffinembedded skeletal muscle followed byperoxidase conjugation of the secondaryantibody and DAB staining. This data demonstrates the use of the GLG1 Antibody (C-term) for immunohistochemistry. Clinicalrelevance has not been evaluated)
GLG1, Polyclonal Antibody (Cat# AAA28703)
Full Name
GLG1 Antibody (C-term)
Gene Names
GLG1; CFR-1; ESL-1; MG160; MG-160
Reactivity
Human, mouse (Predicted Reactivity: Chicken, Hamster, Rat)
Applications
Western Blot, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(BCORL1 Antibody (N-term) (AAA28773) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
BCORL1, Polyclonal Antibody (Cat# AAA28773)
Full Name
BCORL1 Antibody (N-term)
Gene Names
BCORL1; BCoR-L1; CXorf10
Reactivity
Human
Applications
Flow Cytometry, Western Blot, Immunohistochemistry
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(AAA30965 at 1/100 staining mouse testis tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Myc, Polyclonal Antibody (Cat# AAA30965)
Full Name
Phospho-Myc (Ser62) Antibody
Gene Names
MYC; MRTL; MYCC; c-Myc; bHLHe39
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
(All lanes : Anti-DHCR7 Antibody (C-term) at 1:2000 dilution Lane 1: Caco2 whole cell lysateLane 2: mouse testis lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (please inquire) at 1/15000 dilution.Observed band size : 55kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
DHCR7, Polyclonal Antibody (Cat# AAA28753)
Full Name
DHCR7 Antibody (C-term)
Gene Names
DHCR7; SLOS
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with mouse Prodh Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
PRODH, Polyclonal Antibody (Cat# AAA28717)
Full Name
PRODH Antibody (Center)
Gene Names
Prodh; Pro1; Pro-1; Ym24d07
Reactivity
Human, mouse
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(SCNN1A Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
SCNN1A, Polyclonal Antibody (Cat# AAA28784)
Full Name
SCNN1A Antibody (Center)
Gene Names
SCNN1A; BESC2; ENaCa; SCNEA; SCNN1; ENaCalpha
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
FCM (Flow Cytometry)
(Overlay histogram showing HeLa cells stained with AAA28782(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AAA28782, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
HNRNPR, Polyclonal Antibody (Cat# AAA28782)
Full Name
HNRNPR Antibody (N-term)
Gene Names
HNRNPR; HNRPR; hnRNP-R
Reactivity
Human
Applications
Immunofluorescence, Western Blot, Immunohistochemistry, Flow Cytometry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
WB (Western Blot)
(Host: RabbitTarget Name: SCD2Sample Tissue: Mouse Pancreas lysatesAntibody Dilution: 1ug/ml)
SCD2, Polyclonal Antibody (Cat# AAA23601)
Full Name
SCD2 Antibody - N-terminal region
Gene Names
Scd2; swty; Scd-2; Mir5114; mir-5114
Reactivity
Mouse (Predicted Species Reactivity: Mouse)
Applications
Western Blot
Purity
Affinity purified
Pricing
Application Data
(Surface Plasmon Resonance Kinetic Characterization of Polyclonal Antibody Affinity. Purified polyclonal antibodies were immobilized on a Protein A/G coated Carterra LSA sensor chip at concentrations of 5, and 50 ug/mL in duplicate. Antibodies on the surface were exposed to interaction with peptides sequentially via microfluidic controlled flow at 333nM peptide concentration for kinetic characterization of the binders for affinity and specificity, followed by curve fitting using the Kinetics software. Kd determinations for both concentrations were averaged and results and standard deviation are shown.)
SLC26A3, Polyclonal Antibody (Cat# AAA23387)
Full Name
SLC26A3 antibody - middle region
Gene Names
SLC26A3; CLD; DRA
Reactivity
Tested Reactivity: Human
Predicted Reactivity: Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep
Predicted Reactivity: Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Purified
Pricing
WB (Western Blot)
(Western blot analysis of extracts from K562 cells(H2O2 treatment), using Phospho-EGFR (Tyr992)[Tyr1016] Ab. The lane on the left was treated with blocking peptide.)
EGFR, Polyclonal Antibody (Cat# AAA30961)
Full Name
Phospho-EGFR (Tyr1016) Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
IHC (Immunohistochemistry)
(AAA30943 at 1/100 staining rat testicular tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
I kappaB epsilon, Polyclonal Antibody (Cat# AAA30943)
Full Name
Phospho-I kappaB epsilon (Ser22) Antibody
Gene Names
NFKBIE; IKBE
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
WB (Western Blot)
TSPAN18, Polyclonal Antibody (Cat# AAA23603)
Full Name
TSPAN18 Antibody - middle region
Gene Names
Tspan18; BB226562; 6720430O15; 2610042G18Rik
Reactivity
Mouse
Applications
Western Blot
Purity
Affinity purified
Pricing
WB (Western Blot)
(Host: RabbitTarget Name: NADSYN1Sample Tissue: Human HCT116 Whole Cell Antibody Dilution: 1ug/ml)
NADSYN1, Polyclonal Antibody (Cat# AAA23597)
Full Name
NADSYN1 Antibody - C-terminal region
Reactivity
Tested Reactivity: Human
Predicted Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Yeast
Predicted Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Yeast
Applications
Western Blot
Purity
Affinity Purified
Pricing
ACADM, Blocking Peptide (Cat# AAA13683)
Full Name
ACADM Immunizing Peptide
Gene Names
ACADM; MCAD; ACAD1; MCADH
Reactivity
Human
Pricing