At AAA Biotech, we offer a comprehensive selection of high-quality recombinant proteins for use in a wide range of research areas, including immunology, neuroscience, stem cell research, cancer research and more. No matter whether you need recombinant proteins for cell expansion, polarization, differentiation, or cell processing applications, we have got you covered.
Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.
Application Data (HTRF assay for AURKB activity 1 uM STK S2 substrate was incubated with different concentrations of AURKB protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 ?M ATP for 1 hour. The 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for AURKB activity 1 uM STK S2 substrate was incubated with different concentrations of AURKB protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 ?M ATP for 1 hour. The 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant AURKB protein gel 10% SDS-PAGE with Coomassie staining MW: 40.5 kDa Purity: >80%)
Application Data (HTRF assay for HIPK2 (1-640) activity 1 uM STK S3 substrate was incubated with different concentrations of HIPK2 (1-640) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for HIPK2 (1-640) activity 1 uM STK S3 substrate was incubated with different concentrations of HIPK2 (1-640) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant HIPK2 (1-640) protein 10% SDS-PAGE with Coomassie staining MW: 69.5 kDa Purity: >95%)
Application Data (HTRF assay for HIPK3 (163-562) activity 1 uM STK S3 substrate was incubated with different concentrations of HIPK3 (163-562) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 ?M ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant HIPK3 (163-562) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 46.6 kDa Purity: >95%)
Application Data (HTRF assay for LCK activity 1 uM TK substrate was incubated with different concentrations of LCK protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 5 nM SEB, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for LCK activity 1 uM TK substrate was incubated with different concentrations of LCK protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 5 nM SEB, 1 mM DTT and 100 uM ATP for 1 hour. The 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant LCK protein 10% SDS-PAGE with Coomassie staining MW: 59.3 kDa Purity: >90%)
Application Data (MTase-Glo assay for PRMT9 activity1uM histone H4 was incubated with different concentrations of PRMT9 protein in 8ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 1?M SAM at room temperature for 1 hour. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence was read after another 30 min incubation. SAH standard curve (0-1uM) was performed following the same protocol.)
Application Data (MTase-Glo assay for PRMT9 activity1uM histone H4 was incubated with different concentrations of PRMT9 protein in 8ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 1?M SAM at room temperature for 1 hour. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence was read after another 30 min incubation. SAH standard curve (0-1uM) was performed following the same protocol.)
Application Data (HTRF assay for Recombinant MBD1-MBD protein activity 1uM substrate DNA (5’-Biotin-GCAGCCGG/5mC/GCGAATC-3’) were incubated with different concentrations of MBD1-MBD protein in HTRF binding buffer contain 50mM HEPES-NaOH pH 7.4,0.1%BSA for 1 hour, Then 10ul detection reagents contain anti-His antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant MRM1-MBD protein 12.5% SDS-PAGE gel stained with Coomassie Blue. MW: 10.11kDa Purity: >95%)
ELISA (ELISA for ADPRHL1/ARH2 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of ADPRHL1/ARH2 was added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
ELISA (ELISA for ADPRHL1/ARH2 activity 25uM NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP1 in ADPR Buffer and incubated for 1 hour at room temperature. After washing, different concentrations of ADPRHL1/ARH2 was added into each well and incubated in reaction buffer for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
Application Data (AMP-Glo assay for UBE2R1 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2R1 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
SDS-PAGE (Recombinant UBE2R1 protein gel. UBE2D3 protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 28.9kDa Purity: >95%)
Application Data (MTase-Glo assay for METTL1/WDR4 Complex methyltransferase activity 1uM Substrate RNA and 1uM SAM was incubated with different concentrations of METTL1/WDR4 Complex in 8ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP at room temperature for 1 hour (0.2 U/?L RRI was added in this system). 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence were read after another 30 min incubation.)
Application Data (MTase-Glo assay for METTL1/WDR4 Complex methyltransferase activity 1uM Substrate RNA and 1uM SAM was incubated with different concentrations of METTL1/WDR4 Complex in 8ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP at room temperature for 1 hour (0.2 U/?L RRI was added in this system). 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added, and luminescence were read after another 30 min incubation.)
SDS-PAGE (Recombinant METTL1/WDR4 protein gel. METTL1/WDR4 protein was run on a 12.5% SDS-PAGE gel and stained with Coomasse Blue. MW: WDR4: 48.8kDa METTL1: 31.47kDa Purity: >90%)
Application Data (ADP-Glo assay for MAP2K7 (MEK7) activity 1 uM JNK2 was incubated with different concentrations of MAP2K7 (MEK7) protein in a 10 ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 uM DTT, 0.1 mg/ml BSA and 100 uM ATP for 1 hour, then add 10 ul ADP-Glo Reagent at 25 degree C for 60min. Then 20 ul Kinase Detection Reagent incubates at 25 degree C for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
SDS-PAGE (Recombinant MAP2K7 (MEK7) protein gel. Recombinant MAP2K7 protein was run on an 10 % SDS-PAGE gel and stained with Coomassie blue. MW: 48.77kDa Purity: > 95%)
Application Data (HTRF assay for PRKCB activity 1uM STK S1 substrate was incubated with different concentrations of PRKCB protein in 10ul reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT, 1×Lipid activator and 100uM ATP for 1 hour. The 10ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for PRKCB activity 1uM STK S1 substrate was incubated with different concentrations of PRKCB protein in 10ul reaction system containing 1×Enzymatic Buffer, 10 mM MgCl2, 1 mM DTT, 1×Lipid activator and 100uM ATP for 1 hour. The 10ul detection reagents containing anti-STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant PRKCB protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 78.3kDa Purity: >95%)
ELISA (ELISA for ZC3HAV1/PARP13 activity NAD-Biotin and Actived DNA were added into ELISA plate (coated with histone H2A and H2B mixture) with PARP13 in ADPR Buffer and incubated for 1 hour at room temperature. Following Streptavidin-HRP and ECL incubation, the plate was read in a luminometer or microtiter-plate reader.)
Application Data (Mass Spec analysis for Recombinant Mononucleosomes H3S10ph (EPL) - biotin Histone H3S10ph (EPL) - biotin protein was analyzed by ESI-TOF mass spectrometry. Expected mass = 15306 Da. Determined mass = 15306 Da.)
Application Data (Recombinant Mononucleosomes H3S10ph (EPL) - biotin, DNA gel Mononucleosomes H3S10ph (EPL) - biotin, were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: Intact mononucleosome H3S10ph - biotin. Lane 3: 601 DNA. Intact mononucleosome H3S10ph - biotin migrated higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wraps histone octamers to form nucleosomes.)
SDS-PAGE (Recombinant Mononucleosomes H3S10ph (EPL) - biotin, SDS PAGE gel 13% SDS-PAGE gel, stained with Coomassie blue. Purity: ? 92%)
Application Data (Mass Spec analysis for Recombinant Mononucleosomes H3R2/8/17 citrul (EPL) Histone H3R2/8/17 citrul (EPL) protein was analyzed by ESI-TOF mass spectrometry. Expected mass = 15229 Da. Determined mass = 15229 Da.)
Application Data (Recombinant Mononucleosomes H3R2/8/17 citrul (EPL) DNA gel Mononucleosomes H3R2/8/17 citrul were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: Intact mononucleosome H3R2/8/17 citrul Lane 3: 601 DNA. Intact mononucleosome H3R2/8/17 citrul migrated higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wraps histone octamers to form nucleosomes.)
SDS-PAGE (Recombinant Mononucleosomes H3R2/8/17 citrul (EPL) SDS PAGE gel 13% SDS-PAGE gel, stained with Coomassie blue. Purity: ? 92%)
Application Data (Recombinant YTHDC2 binding assay 3 uM biotinylated ssDNA m6A oligos (GTTGG/m6A/CTT) were incubated with different concentrations of YTHDC2 protein in a 10 ul system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (Recombinant YTHDC2 binding assay 3 uM biotinylated ssDNA m6A oligos (GTTGG/m6A/CTT) were incubated with different concentrations of YTHDC2 protein in a 10 ul system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution reaction buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant YTHDC2 protein gel 8% SDS-PAGE Coomassie staining MW: 161.5 kDa Purity: >87%)
WB (Western Blot) (Western blot assay for Recombinant Mononucleosomes (H1.2) 2 ug Recombinant Mononucleosomes were incubated with 0, 12.5, 25, 50 ng DOT1L respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 2 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H3K79me1 antibody and H4 antibody, respectively. DOT1L protein and Recombinant Histone Octamers were used as controls. H4 was detected as the loading control. The result shows that mononucleosomes are better than octamers as substrates for DOT1L.)
SDS-PAGE (Recombinant Mononucleosomes (H1.2) protein gel 13% SDS-PAGE with Coomassie Blue staining Purity: ?95%)
Application Data (Recombinant Mononucleosomes (H1.2) DNA gel Recombinant Mononucleosomes (H1.2) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact monnucleosomes (H1.2). Intact mononucleosomes (H1.2) migrated much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wrap histone octamers to form nucleosomes.)
WB (Western Blot) (Western blot for Recombinant KAT1 / HAT1 protein activity 0.5 ug Histone H4 was incubated with 0 (-), 25 (+), 50 (++), 100 (+++) ng Recombinant HAT1 / KAT1 protein respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 20 uM acetyl-CoA for 2 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H4ac-pan antibody . Western blot was used to detect the production of acetylated Histone H4.)
SDS-PAGE (Recombinant KAT1 / HAT1 protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 50.8 kDa Purity: >90%)
Application Data (MTase-Glo Assay for Recombinant SETD3 protein methyltransferase activity 1 uM beta-actin peptide (66-TLKYPIEHGIVTNWD-80) and 1 uM SAM were incubated with different concentrations of recombinant SETD3 protein in an 8 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 1 hr. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence were read after another 30 min incubation. SAH standard curve (0-1 uM) was performed following the same protocol.)
Application Data (MTase-Glo Assay for Recombinant SETD3 protein methyltransferase activity 1 uM beta-actin peptide (66-TLKYPIEHGIVTNWD-80) and 1 uM SAM were incubated with different concentrations of recombinant SETD3 protein in an 8 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 1 hr. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence were read after another 30 min incubation. SAH standard curve (0-1 uM) was performed following the same protocol.)
SDS-PAGE (Recombinant SETD3 protein gel 10% SDS-PAGE Coomassie staining MW: 68.5 kDa Purity: >90%)
Mmp9; Clg4b; Gel B; MMP-9; B/MMP9; AW743869; pro-MMP-9
Purity
>95% by SDS-PAGE
Pricing
What Are Recombinant Proteins?
Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.
The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.
Common Applications of Recombinant Proteins
Studying protein-protein and protein-DNA interactions
Functional assays to study biological pathways
Standard curves in ELISA and other quantitative assays
Use as antigens for antibody production
Development and screening of therapeutic drugs
Biomarker discovery and validation
Cell signaling and immune response studies
Vaccine research and development
Key Features of AAA Biotech’s Recombinant Proteins
High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.
Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.
Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.
Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.
Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.
Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.
Flexible Quantities: Available in various pack sizes to suit different experimental needs.
Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.
Why Buy Recombinant Proteins from AAA Biotech?
At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.
FAQ
1. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product.
2. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
3. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.
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