Application Data
(ADP-Glo assay for MAP2K7 (MEK7) activity 1 uM JNK2 was incubated with different concentrations of MAP2K7 (MEK7) protein in a 10 ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 uM DTT, 0.1 mg/ml BSA and 100 uM ATP for 1 hour, then add 10 ul ADP-Glo Reagent at 25 degree C for 60min. Then 20 ul Kinase Detection Reagent incubates at 25 degree C for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
MAP2K7 recombinant protein
Recombinant MAP2K7 (MEK7) protein
Synonyms
MAP2K7; N/A; Recombinant MAP2K7 (MEK7) protein; Mitogen Activated Protein Kinase Kinase 7; MKK7; Kinase 7; JNKK2; MAPLL7; MEK; MEK7; PRKMK7; SAPKK-4; MAP2K7 recombinant protein
Host
Baculovirus
Form/Format
Recombinant MAP2K7 (MEK7) protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20% glycerol, 0.04% Triton X-100, 0.5 mM TCEP.
Protein Species
Human
Protein Details
Recombinant MAP2K7 (MEK7) protein was expressed in a baculovirus expression system as the full length protein (accession number NP_660186.1) with an N-terminal DYKDDDDK tag. The molecular weight of the protein is 48.77kDa.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Application Data
(ADP-Glo assay for MAP2K7 (MEK7) activity 1 uM JNK2 was incubated with different concentrations of MAP2K7 (MEK7) protein in a 10 ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 uM DTT, 0.1 mg/ml BSA and 100 uM ATP for 1 hour, then add 10 ul ADP-Glo Reagent at 25 degree C for 60min. Then 20 ul Kinase Detection Reagent incubates at 25 degree C for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
Application Data
(ADP-Glo assay for MAP2K7 (MEK7) activity 1 uM JNK2 was incubated with different concentrations of MAP2K7 (MEK7) protein in a 10 ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 uM DTT, 0.1 mg/ml BSA and 100 uM ATP for 1 hour, then add 10 ul ADP-Glo Reagent at 25 degree C for 60min. Then 20 ul Kinase Detection Reagent incubates at 25 degree C for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
SDS-PAGE
(Recombinant MAP2K7 (MEK7) protein gel. Recombinant MAP2K7 protein was run on an 10 % SDS-PAGE gel and stained with Coomassie blue. MW: 48.77kDa Purity: > 95%)
SDS-PAGE
(Recombinant MAP2K7 (MEK7) protein gel. Recombinant MAP2K7 protein was run on an 10 % SDS-PAGE gel and stained with Coomassie blue. MW: 48.77kDa Purity: > 95%)
Related Product Information for MAP2K7 recombinant protein
MAP2K7 (Dual specificity mitogen-activated protein kinase kinase 7), also known as JNKK2, MAPKK7, MEK, MEK 7, MKK7, PRKMK7, SAPKK-4 and SAPKK4. MAP2K7 is a dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by pro-inflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE.
Product Categories/Family for MAP2K7 recombinant protein
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Product Notes
The MAP2K7 (Catalog #AAA60411) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MAP2K7, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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