Recombinant Proteins

At AAA Biotech, we offer a comprehensive selection of high-quality recombinant proteins for use in a wide range of research areas, including immunology, neuroscience, stem cell research, cancer research and more. No matter whether you need recombinant proteins for cell expansion, polarization, differentiation, or cell processing applications, we have got you covered.

Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.

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Application Data (Recombinant JARID1B / KDM5B protein activity assay. 1 ?M H3K4me3 peptide was incubated with different concentrations of JARID1B / KDM5B protein in 10 ?l reaction system, then 10 ?l anti-H3K4me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Application Data (Recombinant JARID1B / KDM5B protein activity assay. 1 ?M H3K4me3 peptide was incubated with different concentrations of JARID1B / KDM5B protein in 10 ?l reaction system, then 10 ?l anti-H3K4me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant JARID1B / KDM5B protein gel. JARID1B / KDM5B protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue. MW: 179.9 kDa Purity: > 70%)

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JARID1B/KDM5B, Recombinant Protein (Cat# AAA59680)

Full Name

Recombinant JARID1B/KDM5B protein

Gene Names

KDM5B; CT31; PLU1; PUT1; PLU-1; JARID1B; FLJ10538; FLJ12459; FLJ12491; FLJ16281; FLJ23670; RBBP2H1A

Purity

The recombinant protein is >75% pure by SDS-PAGE.

Pricing

$765/0.02 mg $5,580/1 mg $10,880/2x1 mg $15,900/3x1 mg $20,635/4x1 mg $24,855/5x1 mg

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Application Data (HTRF Assay for Recombinant BRD1 (556-688) activity. 3.3 uM histone peptide H4K5/8/12/16 (4Ac) was incubated with BRD1 (556-688) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)

SDS-PAGE (Recombinant BRD1 (556-688) protein gel. BRD1 (556-688) protein was run on an SDS-PAGE gel and stained with Coomassie Blue.)

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BRD1, Recombinant Protein (Cat# AAA59683)

Full Name

Recombinant BRD1 (556-688) protein

Gene Names

BRD1; BRL; BRPF1; BRPF2

Purity

The recombinant protein is >86% pure by SDS-PAGE.

Pricing

$690/0.1 mg $3,330/1 mg $6,485/2x1 mg $9,480/3x1 mg $12,305/4x1 mg $14,730/5x1 mg

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SDS-PAGE (NSD2 (MMSET) activity assay. 2 ug recombinant nucleosomes were incubated with NSD2 in 30 ul reaction system for 3 hr. at room temp. Reaction products were run on a 12.5% SDS-PAGE gel and detected with H3K36me2 antibody. NSD2 only was used as negative control. Results show that nucleosomes are better than octamers as substrates for NSD2. )

SDS-PAGE (Recombinant NSD2 (MMSET) protein gel. NSD2 (MMSET) protein was run on a 8% SDS-PAGE gel and stained with Coomassie blue. MW: 154 kDa Purity: > 75%)

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NSD2, Recombinant Protein (Cat# AAA59692)

Full Name

Recombinant NSD2 (MMSET) protein

Purity

The recombinant protein is >= 75% pure by SDS-PAGE.

Pricing

$715/0.02 mg $5,580/1 mg $10,880/2x1 mg $15,900/3x1 mg $20,635/4x1 mg $24,855/5x1 mg

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Application Data (JMJD2C / KDM4C activity assay.1 uM H3K9me3 peptide was incubated with different concentrations of JMJD2C / KDM4C protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate, 25 uM (NH4)2Fe(SO4)2·6H2O for 1 hour, then 10 ul H3K9me2 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant JMJD2C / KDM4C protein gel. JMJD2C / KDM4C protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 126.1 kDa Purity: > 90%)

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JMJD2C/KDM4C, Recombinant Protein (Cat# AAA59695)

Full Name

Recombinant JMJD2C/KDM4C protein

Purity

The recombinant protein is >95% pure by SDS-PAGE.

Pricing

$765/0.02 mg $5,155/1 mg $10,050/2x1 mg $14,680/3x1 mg $19,060/4x1 mg $22,940/5x1 mg

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Application Data (HTRF assay for UTX / KDM6A activity 3 uM H3K27me3 peptide was incubated with different concentrations of UTX / KDM6A protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate and 25 uM (NH4)2Fe(SO4)2·6H2O for 1 hr, then 10 ul of H3K27me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Application Data (HTRF assay for UTX / KDM6A activity 3 uM H3K27me3 peptide was incubated with different concentrations of UTX / KDM6A protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate and 25 uM (NH4)2Fe(SO4)2·6H2O for 1 hr, then 10 ul of H3K27me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant UTX / KDM6A protein gel. UTX / KDM6A protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 158.8 kDa Purity: >70%)

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UTX/KDM6A, Recombinant Protein (Cat# AAA59697)

Full Name

Recombinant UTX/KDM6A protein

Pricing

$765/0.02 mg $1,495/2x0.02 mg $2,185/3x0.02 mg $2,835/4x0.02 mg $3,205/5x0.02 mg

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WB (Western Blot) (Histone Methyltransferase (HMT) Assay comparing Recombinant Polynucleosomes (H3.3) and histone octamers as substrates. 2 ug Recombinant Polynucleosomes (H3.3) were incubated with 0 ug , 0.25 ug, 0.5 ug and 1 ug NSD2-SET in reaction buffer for 3 hours at room temperature, respectively. Western Blot was used to detect the generation of reaction products (H3K36me2). NSD2-SET only and polynucleosomes only were used as negative control. The Western Blot result shows that polynucleosomes are more suitable substrate for NSD2 than histone octamers.)

Application Data (Recombinant Polynucleosomes (H3.3) protein DNA Gel-shift assay Polynucleosomes (H3.3) and free plasmid DNA were run on 1% agarose gel and stained with ethidium bromide. Intact polynucleosomes migrate much higher than free DNA, thus the DNA resolves at a higher molecular weight when nucleosome-bound.)

SDS-PAGE (Recombinant Polynucleosomes (H3.3) protein gel. Recombinant Polynucleosomes (H3.3) were run on a 12% SDS-PAGE gel and stained with Coomassie Blue.)

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Polynucleosomes, Recombinant Protein (Cat# AAA59702)

Full Name

Recombinant Polynucleosomes (H3.3)

Purity

The recombinant protein is >95% pure by SDS-PAGE.

Pricing

$725/0.02 mg $5,580/1 mg $10,880/2x1 mg $15,900/3x1 mg $20,635/4x1 mg $24,855/5x1 mg

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Application Data (NSD1-SET activity assay using Recombinant Nucleosomes as substrates. Recombinant Nucleosomes were used as substrates in an assay measuring the methyltransferase activity of NSD1-SET. Activity was detected by fluorography.)

SDS-PAGE (Recombinant NSD1-SET protein gel. Recombinant NSD1-SET run on an SDS-PAGE gel and stained with Coomassie blue.)

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NSD1-SET, Recombinant Protein (Cat# AAA59705)

Full Name

Recombinant NSD1-SET protein

Gene Names

NSD1; STO; KMT3B; SOTOS; ARA267; SOTOS1

Purity

The recombinant protein is >95% pure by SDS-PAGE.

Pricing

$690/0.05 mg $4,610/1 mg $8,985/2x1 mg $13,125/3x1 mg $17,035/4x1 mg $20,480/5x1 mg

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Application Data (Recombinant SETD7 protein activity assay. 2 ug Calf Thymus Octamers was incubated with 0.5 ug SETD7 protein in reaction buffer containing radioactive 3H-SAM for 2 hour at room temperature. Activity was detected by fluorography.)

Application Data (Recombinant SETD7 protein HTRF activity assay. 1 ?M H3K4me0 (1-21aa) peptide was incubated with SETD7 in reaction buffer for 2 hour at room temperature. SETD7 enzyme was used in a HTRF assay to determine enzyme linearity. Methylated peptide (H3K4me1) was measured using H3K4me1-specific antibody.)

SDS-PAGE (Recombinant SETD7 protein gel. SETD7 protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)

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SETD7, Recombinant Protein (Cat# AAA59717)

Full Name

Recombinant SETD7 protein

Gene Names

SETD7; KMT7; SET7; SET9; SET7/9

Purity

The recombinant protein is >80% pure by SDS-PAGE.

Pricing

$725/0.02 mg $1,415/2x0.02 mg $2,070/3x0.02 mg $2,680/4x0.02 mg $3,035/5x0.02 mg

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Application Data (HTRF assay for SIRT1 (193-741) activity1 ?M H3K9ac (1-21aa.) peptide was incubated with different concentrations of SIRT1 (193-741) protein in 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA and 50 ?M NAD+ for 30 min at room temperature, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000.)

Application Data (HTRF assay for SIRT1 (193-741) activity1 ?M H3K9ac (1-21aa.) peptide was incubated with different concentrations of SIRT1 (193-741) protein in 10 ?l reaction system containing 25 mM Tris-HCl pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 0.1 mg/ml BSA and 50 ?M NAD+ for 30 min at room temperature, then 10 ?l anti-H3K9me0 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000.)

SDS-PAGE (Recombinant SIRT1 (193-741)12.5% SDS-PAGE with Coomassie Blue staining.MW: 101.5 kDaPurity: >80%)

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SIRT1, Recombinant Protein (Cat# AAA59738)

Full Name

Recombinant SIRT1 (193-741) protein

Purity

Recombinant SIRT1 (193-741) protein that includes amino acids 193-741 of human SIRT1 protein (accession number NM_012238.4) was expressed in E Coli and contains an N-terminal GST-Tag with a molecular weight of 101.5 kDa.The purity ofThe protein is > 80% b

Pricing

$725/0.1 mg $4,000/1 mg $7,795/2x1 mg $11,390/3x1 mg $14,785/4x1 mg $17,745/5x1 mg

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Application Data (HTRF assay for BRD4 (44-460), GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD4 (44-460), GST-tag protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Application Data (HTRF assay for BRD4 (44-460), GST-Tag activity 1 uM H4K5/8/12/16(ac4) peptide was incubated with different concentrations of BRD4 (44-460), GST-tag protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant BRD4 (44-460), GST-Tag protein gel 10% SDS-PAGE Coomassie staining MW: 73.2 kDa Purity: >88%)

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BRD4, Recombinant Protein (Cat# AAA60164)

Full Name

Recombinant BRD4 (44-460) protein, GST-Tag

Gene Names

BRD4; CAP; MCAP; HUNK1; HUNKI

Pricing

$690/0.1 mg $3,330/1 mg $6,485/2x1 mg $9,480/3x1 mg $12,305/4x1 mg $14,730/5x1 mg

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WB (Western Blot) (Recombinant p300 protein activity assay 0.5 ug of Histone H4 were incubated with 0 (-), 12.5 (+), 25 (++), or 50 (+++) ng of p300 protein, respectively in a 20 ul reaction system including 50 mM Tris pH 8.0, 1 mM TCEP, 0.1 mM EDTA, 50 ng/ul BSA and 20 uM acetyl-CoA for 30 minutes at 30 degree C. For each reaction, 7.5 ul product was run on a 12.5% SDS-PAGE gel and detected by Western Blot. p300 only was used as negative control. H4 pan-acetyl antibody was used to recognize products.)

SDS-PAGE (Recombinant p300 protein gel 8% SDS-PAGE Coomassie staining MW: 265.4 kDa Purity: >75% )

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p300, Recombinant Protein (Cat# AAA60167)

Full Name

Recombinant p300 protein

Gene Names

EP300; p300; KAT3B; RSTS2

Pricing

$850/0.01 mg $5,580/0.1 mg $10,880/2x0.1 mg $15,900/3x0.1 mg $20,635/4x0.1 mg $24,855/5x0.1 mg

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Application Data (Recombinant MLLT3 / AF9 (1-138) protein, His / DYKDDDDK-Tag activity assay 3 uM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of MLLT3 / AF9 (1-138) protein, in a 10 ul system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hr, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Application Data (Recombinant MLLT3 / AF9 (1-138) protein, His / DYKDDDDK-Tag activity assay 3 uM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of MLLT3 / AF9 (1-138) protein, in a 10 ul system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hr, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant MLLT3 / AF9 (1-138) protein, His / DYKDDDDK-Tag 10% SDS-PAGE Coomassie staining MW: 20.8 kDa Purity: >79%)

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MLLT3/AF9, Recombinant Protein (Cat# AAA60171)

Full Name

Recombinant MLLT3/AF9 (1-138) protein, His/DYKDDDDK Tag

Gene Names

MLLT3; AF9; YEATS3

Pricing

$690/0.02 mg $1,345/2x0.02 mg $1,960/3x0.02 mg $2,545/4x0.02 mg $2,860/5x0.02 mg

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Application Data (Recombinant YEATS4 / GAS41 protein activity assay 3 uM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS4 / GAS41 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Application Data (Recombinant YEATS4 / GAS41 protein activity assay 3 uM H3K27cr (crotonylated Lysine 27) peptide was incubated with different concentrations of YEATS4 / GAS41 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in binding buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant YEATS4 / GAS41 protein 12.5% SDS-PAGE Coomassie staining MW: 31 kDa Purity: > 81%)

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YEATS4, Recombinant Protein (Cat# AAA60172)

Full Name

Recombinant YEATS4 (GAS41) protein

Gene Names

YEATS4; YAF9; GAS41; NUBI-1; 4930573H17Rik; B230215M10Rik

Pricing

$725/0.1 mg $4,000/1 mg $7,795/2x1 mg $11,390/3x1 mg $14,785/4x1 mg $17,745/5x1 mg

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Application Data (HTRF assay for EGFR (672-1210, L858R) protein activity 1 micro;M TK substrate was incubated with different concentrations of EGFR (672-1210, L858R) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody and SA-XL665 (each of which was 1:100 diluted with 1× Detection Buffer) were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF KinEASE TK assay was used to detect the enzymatic activity.)

Application Data (HTRF assay for EGFR (672-1210, L858R) protein activity 1 micro;M TK substrate was incubated with different concentrations of EGFR (672-1210, L858R) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 5 nM SEB and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody and SA-XL665 (each of which was 1:100 diluted with 1× Detection Buffer) were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF KinEASE TK assay was used to detect the enzymatic activity.)

SDS-PAGE (Recombinant EGFR (672-1210, L858R) protein 10% SDS-PAGE Coomassie staining MW: 63.1 kDa Purity: >52%)

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EGFR, Recombinant Protein (Cat# AAA60175)

Full Name

Recombinant EGFR protein (672-1210, L858R) protein

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61

Pricing

$690/0.02 mg $1,345/2x0.02 mg $1,960/3x0.02 mg $2,545/4x0.02 mg $2,860/5x0.02 mg

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Application Data (HTRF assay for LATS1 protein activity 1 uM STK S1 substrate was incubated with different concentrations of LATS1 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hr. Then 10 ul detection reagents containing TK antibody and SA-XL665 (each of which was 1:100 diluted with 1× Detection Buffer) were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature.HTRF KinEASE STK assay was used to detect the enzymatic activity.)

Application Data (HTRF assay for LATS1 protein activity 1 uM STK S1 substrate was incubated with different concentrations of LATS1 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hr. Then 10 ul detection reagents containing TK antibody and SA-XL665 (each of which was 1:100 diluted with 1× Detection Buffer) were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature.HTRF KinEASE STK assay was used to detect the enzymatic activity.)

SDS-PAGE (Recombinant LATS1 protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 128.1 kDa Purity: >73%)

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LATS1, Recombinant Protein (Cat# AAA60177)

Full Name

Recombinant LATS1 protein

Gene Names

LATS1; wts; WARTS

Pricing

$690/0.02 mg $1,345/2x0.02 mg $1,960/3x0.02 mg $2,545/4x0.02 mg $2,860/5x0.02 mg

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Application Data (HTRF assay for Recombinant PDK1 protein, GST-Tag 1 uM STK S3 substrate was incubated with different concentrations of PDK1 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 2 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.)

Application Data (HTRF assay for Recombinant PDK1 protein, GST-Tag 1 uM STK S3 substrate was incubated with different concentrations of PDK1 protein in 10 ul reaction system containing 1×Enzymatic Buffer, 2 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.)

SDS-PAGE (Recombinant PDK1 protein, GST-Tag 10% SDS-PAGE Coomassie staining MW: 89.8 kDa Purity: >70%)

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PDK1, Recombinant Protein (Cat# AAA60178)

Full Name

Recombinant PDK1 protein, GST-Tag

Gene Names

PDPK1; PDK1; PDPK2; PDPK2P; PRO0461

Pricing

$690/0.02 mg $5,155/1 mg $10,050/2x1 mg $14,680/3x1 mg $19,060/4x1 mg $22,940/5x1 mg

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WB (Western Blot) (Recombinant KAT6B / MORF (718-1008) protein activity assay 0.5 ug Histone H3.1 was incubated with 0 ug (-), 0.1 ug (+), 0.2 ug (++), 0.4 ug (+++) KAT6B / MORF (718-1008) protein in a 20 ul reaction system containing 50 mM Tris-HCl pH 8.6, 2 mM MgCl2, 1 mM TCEP, 0.02% Triton X-100 and 20 uM Acetyl-CoA for 2 hr at RT. Reaction products were detected by Western blot using H3ac (pan-acetyl) antibody .)

SDS-PAGE (Recombinant KAT6B / MORF (718-1008) protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 35.7 kDa Purity: > 93%)

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KAT6B/MORF, Recombinant Protein (Cat# AAA60181)

Full Name

Recombinant KAT6B/MORF (718-1008) protein

Gene Names

KAT6B; qkf; MORF; MOZ2; GTPTS; MYST4; ZC2HC6B; querkopf

Pricing

$725/0.02 mg $5,155/1 mg $10,050/2x1 mg $14,680/3x1 mg $19,060/4x1 mg $22,940/5x1 mg

SDS-PAGE (Recombinant Estrogen Receptor-alpha protein gel 9% SDS-PAGE gel with Coomassie blue staining MW: 67.5 kDa Purity: >80%)

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Estrogen Receptor a, Recombinant Protein (Cat# AAA60203)

Full Name

Recombinant Estrogen Receptor a protein

Gene Names

ESR1; ER; ESR; Era; ESRA; NR3A1

Pricing

$700/0.02 mg $5,155/1 mg $10,050/2x1 mg $14,680/3x1 mg $19,060/4x1 mg $22,940/5x1 mg

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Application Data (MTase-Glo Assay for Recombinant SETD6 protein methyltransferase activity 1 uM substrate peptide and 1 uM SAM were incubated with different concentrations of recombinant SETD6 protein in an 8 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 1 hr. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence was read after another 30 min incubation. SAH standard curve (0-1 uM) was performed following the same protocol)

Application Data (MTase-Glo Assay for Recombinant SETD6 protein methyltransferase activity 1 uM substrate peptide and 1 uM SAM were incubated with different concentrations of recombinant SETD6 protein in an 8 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP for 1 hr. 5×MTase-Glo Reagent was added to the products and incubated for 30 min. Then MTase-Glo Detection was added and luminescence was read after another 30 min incubation. SAH standard curve (0-1 uM) was performed following the same protocol)

SDS-PAGE (Recombinant SETD6 protein gel 10% SDS-PAGE with Coomassie staining. MW: 52.1 kDa Purity: >90%)

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SETD6, Recombinant Protein (Cat# AAA60205)

Full Name

Recombinant SETD6 protein

Pricing

$700/0.02 mg $5,155/1 mg $10,050/2x1 mg $14,680/3x1 mg $19,060/4x1 mg $22,940/5x1 mg

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SDS-PAGE (Streptavidin pull down assay for Recombinant Mononucleosomes H3.3 (R8C) - biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1 hr at 4 degree C. Streptavidin beads were washed 3 times with 1 ml binding buffer. Then the beads were added 60 ul 2×SDS loading buffer and boiled for 10 min at 95 degree C. 2.4 ul samples were loaded and run on a 12.5% SDS-PAGE gel and stained by Commassie blue. The SDS-PAGE gel result showed that almost all of biotinylated mononucleosomes were pulled down by streptavidin beads.)

SDS-PAGE (Recombinant Mononucleosomes H3.3 (R8C) - biotin, protein gel 12.5% SDS-PAGE gel with Coomassie blue staining MW: 108 kDa Purity: >92%)

Application Data (Recombinant Mononucleosomes H3.3 (R8C) - biotin, DNA gel Recombinant Mononucleosomes H3.3 (R8C), biotin, were run on a 2 agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: Free 601 DNA which was used for assembly of nucleosome. Lane 3: Intact mononucleosomes H3.3 (R8C), biotin. Intact mononucleosomes H3.3 (R8C) migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrapped histone octamers to form nucleosomes.)

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Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60207)

Full Name

Recombinant Mononucleosomes H3.3 (R8C)-biotin

Gene Names

H3F3A; H3F3; H3.3A

Pricing

$745/0.02 mg $6,325/1 mg $12,330/2x1 mg $18,020/3x1 mg $23,390/4x1 mg $28,205/5x1 mg

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Application Data (HTRF for IGF2BP2 activity 1 uM ACTB zipcode ssDNA (1212-1313) was incubated with different concentrations of IGF2BP2 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Application Data (HTRF for IGF2BP2 activity 1 uM ACTB zipcode ssDNA (1212-1313) was incubated with different concentrations of IGF2BP2 protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul anti-DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection. .)

SDS-PAGE (Recombinant IGF2BP2 protein gel 10% SDS-PAGE with Coomassie blue staining MW: 67.4 kDa Purity: >88%)

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IGF2BP2, Recombinant Protein (Cat# AAA60210)

Full Name

Recombinant IGF2BP2 protein

Gene Names

IGF2BP2; IMP2; IMP-2; VICKZ2

Pricing

$700/0.02 mg $5,155/1 mg $10,050/2x1 mg $14,680/3x1 mg $19,060/4x1 mg $22,940/5x1 mg

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Application Data (HTRF assay for SETD1A-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1A-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection)

Application Data (HTRF assay for SETD1A-SET Complex activity 1 uM H3 (1-21) was incubated with different concentrations of SETD1A-SET Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 100 uM SAM for 2 hour, then 10 ul H3K4me2 antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection)

SDS-PAGE (Recombinant SETD1A-SET Complex 10% SDS-PAGE with Coomassie blue staining MW of SETD1A-SET: 35.2 kDa WDR5 of 36.6 kDa RBBP5 of 59.2 kDa ASH2L of 68.7 kDa Purity: >95%)

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SETD1A-SET, Recombinant Protein (Cat# AAA60222)

Full Name

Recombinant SETD1A-SET complex

Pricing

$700/0.02 mg $5,155/1 mg $10,050/2x1 mg $14,680/3x1 mg $19,060/4x1 mg $22,940/5x1 mg

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WB (Western Blot) (Recombinant Mononucleosomes H3K27ac - biotin tested by Western blot Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes (H3K27ac) - biotinylated (Lane 2) were detected with anti-H3K27ac antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes (H3K27ac) - biotinylated can be detected by anti-H3K27ac antibody.)

Application Data (Recombinant Mononucleosomes H3K27ac - biotin, DNA gel. Mononucleosomes H3K27ac - biotin were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact mononucleosomes H3K27ac. Intact mononucleosomes.H3K27ac migrated much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wrap histone octamers to form nucleosomes.)

Mass Spec (Mass Spec analysis for Recombinant Mononucleosomes H3K27ac - biotin. Synthetic H3K27ac protein was analyzed by ESI-TOF mass spectrometry. Expected mass = 15313.8 Da. Determined mass = 15313.8 Da.)

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Mononucleosomes H3K27ac, Recombinant Protein (Cat# AAA60099)

Full Name

Recombinant Mononucleosomes H3K27ac-biotin

Gene Names

HIST1H2AC; H2A/l; H2AFL; dJ221C16.4

Pricing

$690/0.01 mg $1,345/2x0.01 mg $1,960/3x0.01 mg $2,545/4x0.01 mg $2,860/5x0.01 mg

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WB (Western Blot) (Western Blot analysis for Recombinant Mononucleosomes H3.3 (K27M). 1 ug PRC2 Complex was incubated with 2 ug wild type Mononucleosomes H3.3 or K27M mutant Mononucleosomes H3.3, respectively, in 30 ul reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 h at room temperature. 6 ul reaction products were loaded and run on a 12.5% SDS-PAGE gel. Western blot was used to detect the generation of reaction products (Anti-H3K27me3). The Western blot result shows that K27M mutation inhibits the activity of PRC2 Complex.)

Application Data (Recombinant Mononucleosomes H3.3 (K27M) - biotin, DNA gel. Recombinant Mononucleosomes H3.3 (K27M) - biotinylated were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: Intact monnucleosomes H3.3 (K27M). Lane 3: 601 DNA. Intact mononucleosomes H3.3 (K27M) migrated much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wrap histone octamers to form nucleosomes.)

SDS-PAGE (Streptavidin pull-down for Recombinant Mononucleosomes H3.3 (K27M) - biotin. Recombinant Mononucleosomes H3.3 (K27M) - biotinylated were pulled down by streptavidin beads. Input nucleosomes (Lane 2) and the nucleosomes (Lane 3) pulled down by streptavidin were run on a 13% SDS-PAGE gel and stained with Commassie blue. The SDS-PAGE gel result shows that almost all of biotinylated mononucleosomes H3.3 (K27M) are pulled down by streptavidin beads. * indicates streptavidin.)

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Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60101)

Full Name

Recombinant Mononucleosomes H3.3 (K27M)-biotin

Gene Names

HIST1H2AC; H2A/l; H2AFL; dJ221C16.4

Purity

The recombinant protein is >=95% by SDS-PAGE gel.

Pricing

$715/0.02 mg $1,395/2x0.02 mg $2,035/3x0.02 mg $2,645/4x0.02 mg $2,970/5x0.02 mg

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WB (Western Blot) (Histone methyltransferase activity assay comparing recombinant nucleosomes and histone octamers as substrates. 2 ug biotinylated mononucleosomes H3.3 (G34V) were incubated with 0.2 ug DOT1L (1-420) in 30 ul reaction system containing 50 mM Tris-HCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 50 uM SAM for 3 hr at room temperature. 6 ul reaction products were loaded and run on a 12.5% SDS-PAGE gel. Western Blot was used to detect the generation of reaction products (Anit-H3K79me1). DOT1L only was used as negative control.)

Application Data (Recombinant Mononucleosomes H3.3 (G34V) - biotin, DNA gel. Recombinant Mononucleosomes H3.3 (G34V) - biotinylated were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact monnucleosomes H3.3 (G34V). Intact mononucleosomes H3.3 (G34V) migrated much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wrap histone octamers to form nucleosomes.)

SDS-PAGE (Streptavidin pull-down for Recombinant Mononucleosomes H3.3 (G34V) – biotin. Recombinant Mononucleosomes H3.3 (G34V) - biotinylated were pulled down by streptavidin beads. Input nucleosomes (Lane 2) and the nucleosomes (Lane 3) pulled down by streptavidin were run on a 12.5% SDS-PAGE gel and stained with Coomassie blue. The SDS-PAGE gel result shows that almost all of biotinylated mononucleosomes H3.3 (G34V) are pulled down by streptavidin beads. * indicates streptavidin.)

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Mononucleosomes H3.3, Recombinant Protein (Cat# AAA60103)

Full Name

Recombinant Mononucleosomes H3.3 (G34V)-biotin

Gene Names

HIST1H2AC; H2A/l; H2AFL; dJ221C16.4

Purity

The recombinant protein is >=95% pure by SDS-PAGE gel analysis.

Pricing

$715/0.02 mg $1,395/2x0.02 mg $2,035/3x0.02 mg $2,645/4x0.02 mg $2,970/5x0.02 mg

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WB (Western Blot) (Recombinant SUV420H2 (2-281) activity assay Recombinant Nucleosomes (H3.1) were incubated with SUV420H2 (2-281) protein. Reaction products were run on SDS-PAGE, and detected by Western Blot using Anti-H4K20me3.Results show the activity of SUV420H2 (2-281) with nucleosomes as substrate is better than with histone octamers.)

SDS-PAGE (Recombinant SUV420H2 (2-281) protein 10% SDS-PAGE Coomassie staining MW: 58.1 kDa Purity: > 90%)

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SUV420H2, Recombinant Protein (Cat# AAA60106)

Full Name

Recombinant SUV420H2 (2-281) protein

Gene Names

SUV420H2; KMT5C

Pricing

$715/0.05 mg $4,000/1 mg $7,795/2x1 mg $11,390/3x1 mg $14,785/4x1 mg $17,745/5x1 mg

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WB (Western Blot) (Western Blot analysis for Recombinant Mononucleosomes H3K9ac (EPL) - biotin. Recombinant Mononucleosomes H3K9ac(EPL) - biotinylated (Lane 1) and unmodified nucleosomes (Lane 2) were detected with anti-H3K9ac antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3K9ac (EPL) - biotin can be detected by anti-H3K9ac antibody.)

Application Data (Recombinant Mononucleosomes H3K9ac (EPL) - biotin, DNA gel. Recombinant Mononucleosomes H3K9ac (EPL) - biotin were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA. Lane 3: Intact monnucleosomes H3K9ac. Intact mononucleosomes H3K9ac migrated much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wrap histone octamers to form nucleosomes.)

SDS-PAGE (Streptavidin pull-down for Recombinant Mononucleosomes H3K9ac (EPL) - biotin. Recombinant Mononucleosomes H3K9ac (EPL) - biotin were pulled down by streptavidin beads. Input nucleosomes (Lane 2) and the nucleosomes (Lane 3) pulled down by streptavidin were run on a 13% SDS-PAGE gel and stained with Commassie blue. The SDS-PAGE gel result shows that most of biotinylated mononucleosomes H3K9ac are pulled down by streptavidin beads. * indicates streptavidin.)

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Mononucleosomes H3K9ac, Recombinant Protein (Cat# AAA60108)

Full Name

Recombinant Mononucleosomes H3K9ac (EPL)-biotin

Gene Names

HIST1H2AC; H2A/l; H2AFL; dJ221C16.4

Purity

The recombinant protein is >=95% by SDS-PAGE gel.

Pricing

$690/0.02 mg $1,345/2x0.02 mg $1,960/3x0.02 mg $2,545/4x0.02 mg $2,860/5x0.02 mg

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Application Data (Recombinant IDO1 protein activity assay 400 uM L-tryptophan was incubated with different concentrations of IDO1 protein in reaction buffer mixture. The reaction was stopped by the addition of 40 ul 30% (w/v) trichloroacetic acid and heated at 65 degree C for 15 min. After cooling down to 4 degree C, the reaction was centrifuged at 1,125 g for 10 min. 100 ul supernatant was transferred into 96-well plate and mixed with 100 ul of 2% (w/v) p-DMAB. The yellow pigment derived from kynurenine was measured at 490 nm.)

SDS-PAGE (Recombinant IDO1 protein gel 10% SDS-PAGE Coomassie staining MW: 47.5 kDa Purity: > 90%)

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IDO1, Recombinant Protein (Cat# AAA60111)

Full Name

Recombinant IDO1 protein

Gene Names

IDO1; IDO; INDO; IDO-1

Pricing

$700/0.1 mg $4,000/1 mg $7,795/2x1 mg $11,390/3x1 mg $14,785/4x1 mg $17,745/5x1 mg

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Application Data (HTRF assay for NFKB1 / p50 (1-434) activity 1 uM dsDNA oligos (DNA sequence: 5’-ATGGGGATTCCCCGC-3’) were incubated with different concentrations of protein in 10 ul reaction system containing 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 5% glycerol, 0.1% Triton X-100 for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)

Application Data (HTRF assay for NFKB1 / p50 (1-434) activity 1 uM dsDNA oligos (DNA sequence: 5’-ATGGGGATTCCCCGC-3’) were incubated with different concentrations of protein in 10 ul reaction system containing 20 mM Tris-HCl pH 8.0, 150 mM NaCl, 5% glycerol, 0.1% Triton X-100 for 1 hour, then 10 ul GST antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) were added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature.)

SDS-PAGE (Recombinant NFKB1 / p50 (1-434) protein gel 10% SDS-PAGE gel stained with Coomassie blue. MW: 73.9 kDa Purity: ?8 )

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NFkB1 p50, Recombinant Protein (Cat# AAA60112)

Full Name

Recombinant NFkB1 p50 (1-434) protein

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

Pricing

$690/0.05 mg $4,000/1 mg $7,795/2x1 mg $11,390/3x1 mg $14,785/4x1 mg $17,745/5x1 mg

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Application Data (Recombinant Mononucleosomes (H2A.Z) DNA gel Mononucleosomes (H2A.Z) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: free 601 DNA. Lane 3: Intact mononucleosomes. Intact mononucleosomes migrate much higher than free 601 DNA. The agarose gel result shows that almost all of 601 DNA wraps histone octamers to form mononucleosomes)

SDS-PAGE (Recombinant Mononucleosomes (H2A.Z) 13% SDS-PAGE Coomassie staining MW: 108.3 kDa Purity: ? 85%)

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Mononucleosomes, Recombinant Protein (Cat# AAA60120)

Full Name

Recombinant Mononucleosomes (H2A.Z)

Gene Names

H2AFZ; H2AZ; H2A.z; H2A/z; H2A.Z-1

Pricing

$690/0.05 mg $5,660/1 mg $11,035/2x1 mg $16,120/3x1 mg $20,930/4x1 mg $25,215/5x1 mg

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Application Data (HTRF for YTHDC1 (325-502) ActivityOligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDC1 (325-502) protein in a reaction system for 1 hour, then DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Application Data (HTRF for YTHDC1 (325-502) activity Oligo m6A ssDNA (GTTGG/m6A/CTT) was incubated with different concentrations of YTHDC1 (325-502) protein in a reaction system for 1 hour, then DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in the same buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant YTHDC1 (325-502) SDS PAGE gel 13% SDS-PAGE Coomassie staining MW: 24.9 kDa Purity: >90%)

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YTHDC1, Recombinant Protein (Cat# AAA60133)

Full Name

Recombinant YTHDC1 (325-502) protein

Gene Names

YTHDC1; YT521; YT521-B

Pricing

$745/0.1 mg $4,000/1 mg $7,795/2x1 mg $11,390/3x1 mg $14,785/4x1 mg $17,745/5x1 mg

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Application Data (ALKBH2 dioxygenase activity assay 1 ug of ssDNA oligos (5’-AAAGCAG(1mA)ATTCGAAAAAGCGAAA-3’) was incubated with 0 ug, 0.5 ug, 1 ug, 2 ug, 4 ug of ALKBH2 in 50 ul reaction system including 50 mM HEPES-NaOH pH 8.0, 50 ?M Fe(NH4)2(SO4)2, 1 mM 2-oxoglutarate, 2 mM ascorbate and 1 mM TCEP for 30 min at 37 degree C. Then ssDNA oligos were annealed with equimolar of non-methylated complement strand followed by 1 ug EcoRI digestion for 45 min at 37 degree C. 1/4 reaction products were run on a 20% Native PAGE gel and stained by ethidium bromide.)

SDS-PAGE (Recombinant ALKBH2 protein 10% SDS-PAGE Coomassie staining MW: 33.1 kDa Purity: >82%)

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ALKBH2, Recombinant Protein (Cat# AAA60148)

Full Name

Recombinant ALKBH2 protein

Gene Names

ALKBH2; ABH2

Pricing

$745/0.1 mg $4,000/1 mg $7,795/2x1 mg $11,390/3x1 mg $14,785/4x1 mg $17,745/5x1 mg

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Application Data (Recombinant NgTet1 activity assay 1 ug of 30 nt ssDNA oligos with a 5mCpG site were incubated with different concentrations of NgTet1 in a reaction system at 34 degree C for 1 hour. Sample was concentrated to 10 ul, and 1 ul was spotted onto nylon membrane. 5hmC Ab and 5-caC Ab were used to detect products. NgTet1 activity assay was detected by Dot-Blot..)

SDS-PAGE (Recombinant NgTet1 protein 10% SDS-PAGE Coomassie staining MW: 38.7 kDa Purity: >85%)

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NgTet1, Recombinant Protein (Cat# AAA60157)

Full Name

Recombinant NgTet1 (1-321) protein

Pricing

$725/0.1 mg $4,000/1 mg $7,795/2x1 mg $11,390/3x1 mg $14,785/4x1 mg $17,745/5x1 mg

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Application Data (HTRF assay for PLK3 (57-340) activity 1 uM STK S3 substrate was incubated with different concentrations of PLK3 (57-340) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 2 mg/ml CaSein and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Application Data (HTRF assay for PLK3 (57-340) activity 1 uM STK S3 substrate was incubated with different concentrations of PLK3 (57-340) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, 2 mg/ml CaSein and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant PLK3 (57-340) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 33.7 kDa Purity: >85%)

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PLK3, Recombinant Protein (Cat# AAA60243)

Full Name

Recombinant PLK3 (57-340) protein

Gene Names

PLK3; CNK; FNK; PRK

Pricing

$700/0.02 mg $5,155/1 mg $10,050/2x1 mg $14,680/3x1 mg $19,060/4x1 mg $22,940/5x1 mg

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Application Data (HTRF assay for JAK3 (781-1124) activity 1 uM TK substrate was incubated with different concentrations of JAK3 (781-1124) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Application Data (HTRF assay for JAK3 (781-1124) activity 1 uM TK substrate was incubated with different concentrations of JAK3 (781-1124) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant JAK3 (781-1124) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 39.8 kDa Purity: >80%.)

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JAK3, Recombinant Protein (Cat# AAA60244)

Full Name

Recombinant JAK3 (781-1124) protein

Gene Names

JAK3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN

Pricing

$700/0.02 mg $5,155/1 mg $10,050/2x1 mg $14,680/3x1 mg $19,060/4x1 mg $22,940/5x1 mg

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Application Data (HTRF assay for STK11 / LKB1 activity 1 uM STK S1 substrate was incubated with different concentrations of STK11 / LKB1 protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100u?M ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant STK11 / LKB1 protein 10% SDS-PAGEwith Coomassie staining MW: 49.6 kDa Purity: >80%)

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STK11/LKB1, Recombinant Protein (Cat# AAA60249)

Full Name

Recombinant STK11/LKB1 protein

Gene Names

STK11; PJS; LKB1; hLKB1

Pricing

$700/0.02 mg $5,155/1 mg $10,050/2x1 mg $14,680/3x1 mg $19,060/4x1 mg $22,940/5x1 mg

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Application Data (HTRF assay for MAP3K8 / COT (30-397) activity 1 uM STK S3 substrate was incubated with different concentrations of MAP3K8 / COT (30-397) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100 uM ATP for 1 hour. Then 10u?l detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant MAP3K8 / COT (30-397) protein 10% SDS-PAGE with Coomassie staining MW: 42.8 kDa Purity: >50%)

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MAP3K8/COT, Recombinant Protein (Cat# AAA60250)

Full Name

Recombinant MAP3K8 (30-397)/COT protein

Gene Names

MAP3K8; COT; EST; ESTF; TPL2; MEKK8; Tpl-2; c-COT

Pricing

$700/0.02 mg $5,155/1 mg $10,050/2x1 mg $14,680/3x1 mg $19,060/4x1 mg $22,940/5x1 mg

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SDS-PAGE (DNMT1 protein gel. DNMT1 (2.8 ug) run on a 4-20% SDS-PAGE gel and stained with Coomassie Blue. Arrow indicates recombinant DNMT1.)

Application Data (DNMT1 activity assay. Recombinant DNMT1 activity was incubated with 400 uM S-adenosylmethionine (SAM) and DNA substrate, followed by detection with anti-5mC antibody and chemiluminescent detection.)

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DNMT1, Recombinant Protein (Cat# AAA59635)

Full Name

Recombinant DNMT1 protein

Pricing

$745/0.01 mg $1,455/2x0.01 mg $2,125/3x0.01 mg $2,755/4x0.01 mg $3,120/5x0.01 mg

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Application Data (Recombinant CREBBP (1081-1197) HTRF activity assay. 3 uM H4K5/8/12/16 (ac4) peptide was incubated with different concentrations of CREBBP (1081-1197) protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (1:100 dilution in reaction buffer) was added to each reaction system and incubated for 1 hour. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

SDS-PAGE (Recombinant CREBBP (1081-1197) protein gel. CREBBP (1081-1197) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue.)

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CREBBP, Recombinant Protein (Cat# AAA59643)

Full Name

Recombinant CREBBP (1081-1197) protein

Purity

The recombinant protein is >85% pure by SDS-PAGE.

Pricing

$690/0.1 mg $4,000/1 mg $7,795/2x1 mg $11,390/3x1 mg $14,785/4x1 mg $17,745/5x1 mg

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Application Data (HTRF assay for PRC2 Complex activity 1 uM H3 (16-37) peptide was incubated with different concentrations of PRC2 Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 3 hours, then 10 ul of H3K27me1 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at RT. HTRF assay was used for detection.)

Application Data (HTRF assay for PRC2 Complex activity 1 uM H3 (16-37) peptide was incubated with different concentrations of PRC2 Complex in a 10 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 3 hours, then 10 ul of H3K27me1 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at RT. HTRF assay was used for detection.)

SDS-PAGE (Recombinant PRC2 Complex gel. PRC2 Complex was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW as follows: EZH2: 87 kDa. SUZ12: 83 kDa. EED: 50.2 kDa. RbAp46: 47.8 kDa. RbAp48: 47.7 kDa. Purity: > 90%)

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PRC2, Recombinant Protein (Cat# AAA59652)

Full Name

Recombinant PRC2 complex

Gene Names

EZH2; WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A

Pricing

$745/0.02 mg $5,580/1 mg $10,880/2x1 mg $15,900/3x1 mg $20,635/4x1 mg $24,855/5x1 mg

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Application Data (HTRF Assay for Recombinant SMARCA4/BRG1 (1448-1569) Activity. 3.3 uM histone peptide H3K14ac was incubated with SMARCA4/BRG1 (1448-1569) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)

Application Data (HTRF Assay for Recombinant SMARCA4/BRG1 (1448-1569) Activity. 3.3 uM histone peptide H3K14ac was incubated with SMARCA4/BRG1 (1448-1569) in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)

SDS-PAGE (Recombinant SMARCA4 / BRG1 (1448-1569) protein gel. SMARCA4 / BRG1 (1448-1569) protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue.)

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SMARCA4/BRG1, Recombinant Protein (Cat# AAA59660)

Full Name

Recombinant SMARCA4/BRG1 (1448-1569) protein

Gene Names

SMARCA4; BRG1; SNF2; SWI2; MRD16; RTPS2; BAF190; SNF2L4; SNF2LB; hSNF2b; BAF190A

Pricing

$725/0.1 mg $4,000/1 mg $7,795/2x1 mg $11,390/3x1 mg $14,785/4x1 mg $17,745/5x1 mg

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WB (Western Blot) (Western blot for PRMT1 methyltransferase activity 0.5 ug Histone H4 was incubated with 0 ug (-), 0.05 ?g (+), 0.1 ug (++), 0.2 ug (+++) PRMT1 system for 2 hr at room temperature. Products were detected by Western blot using H4R3me2a (asymmetric) antibody.)

SDS-PAGE (Recombinant PRMT1 protein gel. PRMT1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue.)

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PRMT1, Recombinant Protein (Cat# AAA59664)

Full Name

Recombinant PRMT1 protein

Pricing

$690/0.02 mg $1,345/2x0.02 mg $1,960/3x0.02 mg $2,545/4x0.02 mg $2,860/5x0.02 mg

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Application Data (Recombinant DNMT3B protein activity assay Recombinant DNMT3B protein activity was measured using DNMT Activity / Inhibition Assay, for 1 hour at 37 degree C)

SDS-PAGE (Recombinant DNMT3B protein gel. Recombinant DNMT3B protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)

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DNMT3B, Recombinant Protein (Cat# AAA59665)

Full Name

Recombinant DNMT3B protein

Gene Names

DNMT3B; ICF; ICF1; M.HsaIIIB

Purity

The recombinant protein is >60% pure by SDS-PAGE.

Pricing

$745/0.02 mg $5,155/1 mg $10,050/2x1 mg $14,680/3x1 mg $19,060/4x1 mg $22,940/5x1 mg

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DB (Dot Blot) (Dot Blot analysis for recombinant TET3 (824-1795) protein. 250 ng of 30 base oligonucleotide (contains a 5mCpG) was incubated with 0 ug, 312.5 and 625 ng TET3 protein, respectively, in buffer containing 50 mM HEPES pH 7.9, 50 ?M Fe(NH4)2(SO4)2, 1 mM ascorbate, 100 uM alpha-ketoglutarate, 200 uM ATP, 1 mM TCEP at room temperature for 1 hour. Sample was concentrated to 5 ul, then 1 ul sample was spotted onto positively charged nylon membrane. 5-hmC antibody was used to detect the generation of products.)

SDS-PAGE (Recombinant TET3 (824-1795) protein gel. Recombinant TET3 (824-1795) protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)

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Tet3, Recombinant Protein (Cat# AAA59672)

Full Name

Recombinant Tet3 (824-1795) protein

Gene Names

TET3; hCG_40738

Pricing

$690/0.01 mg $1,345/2x0.01 mg $1,960/3x0.01 mg $2,545/4x0.01 mg $2,860/5x0.01 mg

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Application Data (HDAC6 (H230A) Protein activity assay. 3 uM H3K9ac (1-21) peptides was incubated with different concentrations of HDAC6 (H230A) protein in reaction buffer for 30 minutes at 37 degree C followed by developing for 30 minutes at room temperature. Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection.)

Application Data (HDAC6 (H230A) Protein activity assay. 3 uM H3K9ac (1-21) peptides was incubated with different concentrations of HDAC6 (H230A) protein in reaction buffer for 30 minutes at 37 degree C followed by developing for 30 minutes at room temperature. Reaction product was detected by Anti-H3K9me0-Eu antibody. HTRF assay was used for activity detection.)

SDS-PAGE (Recombinant HDAC6 (H230A) protein gel HDAC6 (H230A) protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue.)

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HDAC6, Recombinant Protein (Cat# AAA59753)

Full Name

Recombinant HDAC6 (H230A) protein

Purity

The recombinant protein is >85% pure by SDS-PAGE.

Pricing

$625/0.02 mg $1,210/2x0.02 mg $1,765/3x0.02 mg $2,295/4x0.02 mg $2,550/5x0.02 mg

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WB (Western Blot) (Western Blot analysis for Recombinant Histone H3K79me3 (MLA) Recombinant Histone H3K27me3 (MLA) (Lane 1) and Histone H3.1 (Lane 2) were detected with H3K79me2me3 antibody . Histones stained by coomassie blue below were as loading control.)

SDS-PAGE (Recombinant Histone H3K79me3 (MLA) gel. Histone H3K79me3 (MLA) was run on a 12.5% SDS- PAGE gel and stained with Coomassie Blue. MW: 15.3 kDa Purity: > 90%)

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Histone H3K79me3, Recombinant Protein (Cat# AAA59760)

Full Name

Recombinant Histone H3K79me3 (MLA)

Gene Names

HIST1H3A; H3/A; H3FA

Purity

The recombinant histone is >92% pure by SDS-PAGE.

Pricing

$520/0.1 mg $1,330/1 mg $2,585/2x1 mg $3,770/3x1 mg $4,895/4x1 mg $5,715/5x1 mg

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DB (Dot Blot) (Dot blot for Recombinant ALKBH5 protein activity. 3 uM m6A ssDNA oligos (sequence: 5’-GTTGCCTGTTCGTGTTGG/m6A/CTTGCCTGT-3’) were incubated with different concentrations of ALKBH5 proteins in a 30 ul reaction system including 50 mM HEPES-NaOH pH 7.5, 100 uM 2-oxoglutarate, 100 uM ascorbate, 50 ?M (NH4)2Fe(SO4)2·6H2O and 1 mM TCEP for 2 hours at room temperature. Then each reaction was concentrated to a final volume of 10 ul. 1 ul of each reaction was spotted onto a positively charged nylon membrane and products were detected by Dot blot assay with m6A antibody .)

SDS-PAGE (Recombinant ALKBH5 protein gel Recombinant ALKBH5 was run on a 10% SDS-PAGE gel stained with Coomassie Blue. MW: 45.5 kDa Purity: > 90%)

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ALKBH5, Recombinant Protein (Cat# AAA59767)

Full Name

Recombinant ALKBH5 protein

Gene Names

ALKBH5; ABH5; OFOXD; OFOXD1

Pricing

$745/0.02 mg $1,455/2x0.02 mg $2,125/3x0.02 mg $2,755/4x0.02 mg $3,120/5x0.02 mg

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Application Data (ESI-TOF Mass Spec analysis for Recombinant Histone H3K79me2 (MLA). Expected mass: 15271 Da Observed mass: 15271 Da)

SDS-PAGE (Recombinant Histone H3K79me2 (MLA) protein gel. 12% SDS-PAGE Coomassie staining. Purity: > 85%)

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Histone H3K79me2, Recombinant Protein (Cat# AAA59776)

Full Name

Recombinant Histone H3K79me2 (MLA)

Gene Names

HIST1H3A; H3/A; H3FA

Pricing

$520/0.1 mg $1,330/1 mg $2,585/2x1 mg $3,770/3x1 mg $4,895/4x1 mg $5,715/5x1 mg

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Application Data (Recombinant IDH1 (R131C) protein activity assay 10 uM NADPH and 1 uM a-KG were incubated with 100 nM IDH1 (R132C) protein in 200 ul reaction system containing 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2 and 0.03% BSA (room temperature). Depletion of NADPH was monitored continuously at Abs 340 nm for 20 min.)

SDS-PAGE (Recombinant IDH1 (R132C) protein gel 10% SDS-PAGE Coomassie staining MW: 47.7 kDa Purity: > 90% )

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IDH1, Recombinant Protein (Cat# AAA59783)

Full Name

Recombinant IDH1 (R132C) protein

Pricing

$725/0.1 mg $4,000/1 mg $7,795/2x1 mg $11,390/3x1 mg $14,785/4x1 mg $17,745/5x1 mg

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Application Data (10 uM NADPH and 1 uM α-KG were incubated with 100 nM IDH1 (R132H) protein in 200 ul reaction system containing 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 10 mM MgCl2 and 0.03% BSA (room temperature). Depletion of NADPH was monitored continuously at Abs 340 nm for 45 min.)

SDS-PAGE (Recombinant IDH1 (R132H) protein10% SDS-PAGE Coomassie staining MW: 47.7 kDa Purity: > 90% )

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IDH1, Recombinant Protein (Cat# AAA59784)

Full Name

Recombinant IDH1 (R132H) protein

Pricing

$725/0.1 mg $4,000/1 mg $7,795/2x1 mg $11,390/3x1 mg $14,785/4x1 mg $17,745/5x1 mg

What Are Recombinant Proteins?

Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.

The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.

Common Applications of Recombinant Proteins

• Studying protein-protein and protein-DNA interactions


• Functional assays to study biological pathways


• Standard curves in ELISA and other quantitative assays


• Use as antigens for antibody production


• Development and screening of therapeutic drugs


• Biomarker discovery and validation


• Cell signaling and immune response studies


• Vaccine research and development

Key Features of AAA Biotech’s Recombinant Proteins

• High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.


• Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.


• Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.


• Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.


• Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.


• Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.


• Flexible Quantities: Available in various pack sizes to suit different experimental needs.


• Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.

Why Buy Recombinant Proteins from AAA Biotech?

At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.

With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.

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