At AAA Biotech, we offer a comprehensive selection of high-quality recombinant proteins for use in a wide range of research areas, including immunology, neuroscience, stem cell research, cancer research and more. No matter whether you need recombinant proteins for cell expansion, polarization, differentiation, or cell processing applications, we have got you covered.
Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.
Application Data (HTRF assay for MBD2-MBD activity 1uM substrate DNA (5’-Biotin-GCAGCCGG/5mC/GCGAATC-3’) were incubated with different concentrations of MBD2-MBD protein in HTRF binding buffer contain 50mM HEPES-NaOH pH 7.4,0.1%BSA for 1 hour, Then 10ul detection reagents contain anti-His antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (MBD2-MBD protein gel. 12.5% SDS-PAGE gel stained with Coomassie Blue. MW: 11.19kDa Purity: >95%)
Application Data (HTRF assay for MBD4-MBD activity 1uM substrate DNA (5’-Biotin-GCAGCCGG/5mC/GCGAATC-3’) were incubated with different concentrations of MBD4-MBD protein in HTRF binding buffer contain 50mM HEPES-NaOH pH 7.4,0.1%BSA for 1 hour, Then 10ul detection reagents contain anti-His antibody (1:100) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (MBD4-MBD protein gel. 12.5% SDS-PAGE gel stained with Coomassie Blue. MW: 12.31kDa Purity: >95%)
Application Data (AMP-Glo assay for UBE2A activity 7.9uM ubiquitin, 63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2A in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 3 degree C for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
Application Data (AMP-Glo assay for UBE2E1 activity 7.9uM ubiquitin,63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2E1 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 3 degree C for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
Application Data (HTRF assay for Recombinant NTRK1 (440-796, F589L) activity 1uM TK substrate was incubated with different concentrations of NTRK1 (440-796, F589L) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant NTRK1 (440-796, F589L)protein 10% SDS-PAGE gel stained with Coomassie Blue. MW: 41.32kDa Purity >90%)
Application Data (HTRF assay for NTRK1 (440-796, A608D) activity 1uM TK substrate was incubated with different concentrations of NTRK1 (440-796, A608D) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for NTRK1 (440-796, A608D) activity 1uM TK substrate was incubated with different concentrations of NTRK1 (440-796, A608D) protein in 10ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT and 100uM ATP for 1 hour. Then 10ul detection reagents containing anti-TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (NTRK1 (440-796, A608D) protein gel. 10% SDS-PAGE gel stained with Coomassie Blue. MW: 41.32kDa Purity: >90%)
Application Data (AMP-Glo assay for UBE2R2 activity 7.9uM ubiquitin, 63 nM UBA1 and 25uM ATP were incubated with different concentrations of UBE2R2 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
SDS-PAGE (Recombinant UBE2R2 protein gel. UBE2R2 protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 29.33kDa Purity: >85%)
Application Data (AMP-Glo assay for UBA1 activity 7.9uM ubiquitin and 25uM ATP were incubated with different concentrations of UBA1 in 10ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml BSA at 37? for 1 hour. 10ul of AMP-Glo Reagent I was added to the reaction and incubated for 1 hour at room temperature. Then 20ul of AMP-Glo Detection Solution was added and luminescence was read after another 30 min incubation.)
SDS-PAGE (Recombinant UBA1 protein gel. UBA1 protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 119.12kDa Purity: >95%)
Application Data (HTRF Assay for Recombinant BRD2 activity 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD2 in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF Assay for Recombinant BRD2 activity 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD2 in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF Assay for Recombinant BRD4 activity 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD4 in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. All the operations and reactions were performed at room temperature. HTRF was used to detect the products.)
Application Data (HTRF Assay for Recombinant BRD4 activity 3 uM histone peptide H4K5/8/12/16(ac4) was incubated with BRD4 in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products. All the operations and reactions were performed at room temperature. HTRF was used to detect the products.)
SDS-PAGE (Recombinant BRD4 protein gel 10% SDS-PAGE Coomassie staining MW: 81.7kDa Purity: >95%)
Application Data (ADP-Glo assay for CDK9/CyclinT1 Complex activity 0.2 ug/ul PDKtide peptide substrate was incubated with different concentrations of CDK9/CyclinT1 Complex protein in a 10 ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 uM DTT, 0.1 mg/ml BSA and 100 uM ATP for 1 hour, then add 10 ul ADP-Glo Reagent at RT for 60min.Then add 20 ul Kinase Detection Reagent incubates at RT for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
Application Data (ADP-Glo assay for PI3Ka/p85 Complex activity 0.125 ug/ul PI-3 Kinase Lipid Substrate was incubated with different concentrations of PI3Ka/p85 Complex protein in a 10 ul reaction system containing 40 mM Tris-HCl pH 7.4, 20 mM MgCl2, 50 uM DTT, 0.1 mg/ml BSA and 100 uM ATP for 1 hour, then add 10 ul ADP-Glo Reagent at RT for 60min.Then add 20 ul Kinase Detection Reagent incubates at RT for 60min. All the operations and reactions were performed at RT. Finally, Luminescence measurement is collected by BMG.)
SDS-PAGE (Tau Proteins: Representation of seven Tau proteins, from top to bottom: Tau-441, Tau-410, Tau-412, Tau-381, Tau-383, Tau-352 and Tau-316 (Tau Protein Marker; .)
SDS-PAGE (Purity: SDS-PAGE image of purified Tau Proteins. Lane 1, Tau-441, BRSK2-phosphorylated. Lane 2, Tau-441 Protein)
SDS-PAGE (Tau Proteins: Representation of seven Tau proteins, from top to bottom: Tau-441, Tau-410, Tau-412, Tau-381, Tau-383, Tau-352 and Tau-316 (Tau Protein Marker; .)
SDS-PAGE (Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Purity Data (The purity of Dog ACE2 (18-739) Protein was determined to be >90% by densitometry. approx. MW 150kDa)
Specific Activity (Binding ability measured in a functional ELISA. Unlike human ACE2. Dog ACE2 (18-739) Protein (AAA73166) does not bind well to immoblized 2019-n-Cov Spike protein RBD .)
Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.
The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.
Common Applications of Recombinant Proteins
Studying protein-protein and protein-DNA interactions
Functional assays to study biological pathways
Standard curves in ELISA and other quantitative assays
Use as antigens for antibody production
Development and screening of therapeutic drugs
Biomarker discovery and validation
Cell signaling and immune response studies
Vaccine research and development
Key Features of AAA Biotech’s Recombinant Proteins
High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.
Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.
Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.
Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.
Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.
Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.
Flexible Quantities: Available in various pack sizes to suit different experimental needs.
Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.
Why Buy Recombinant Proteins from AAA Biotech?
At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.
FAQ
1. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product.
2. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
3. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.
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