At AAA Biotech, we offer a comprehensive selection of high-quality recombinant proteins for use in a wide range of research areas, including immunology, neuroscience, stem cell research, cancer research and more. No matter whether you need recombinant proteins for cell expansion, polarization, differentiation, or cell processing applications, we have got you covered.
Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.
Application Data (Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein SPR DataBiotinylated Human Fc gamma RIIIB (NA2) , His Tag captured on CM5 Chip via Anti-His Antibody can bind Rituximab, hFc Tag with an affinity constant of 4.3?M as determined in SPR assay (Biacore T200).)
Application Data (Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein SEC-HPLCThe purity of Biotinylated Human Fc gamma RIIIB (NA2) is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein Tris-Bis PAGEBiotinylated Human Fc gamma RIIIB (NA2) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
ELISA (Biotinylated Human IL-13 Protein ELISA DataImmobilized Biotinylated Human IL-13, His Tag at 2?g/ml (100?l/well) on the streptavidin (5?g/ml) precoated plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 1.4?g/ml determined by ELISA.)
Tris-Bis-PAGE (Biotinylated Human IL-13 Protein Tris-Bis PAGEBiotinylated IL-13 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.)
Application Data (Figure 5: ATAC-Seq Results are Consistent with Pre-indexed Assembled Tn5 Transposomes and Standard Tn5 Transposomes. 50,000 fresh K562 cells were assayed in ATAC-Seq with either Active Motif’s Pre-indexed Assembled Tn5 Transposomes (top four tracks) or standard, non-indexed Tn5 Assembled Transposomes (bottom track). ATAC-Seq results were consistent between both methods. Browser tracks are shown at Chr6:159,668,373-159,821,466.)
SDS-PAGE (Figure 1: 12.5% SDS PAGE Gel of Recombinant Tn5 Transposase Protein (MW 53kDa).)
WB (Western Blot) (Western blot assay for Recombinant Mononucleosomes H3.1 (K27M) - biotin 2 ug Recombinant Mononucleosomes H3.1 (K27M) - biotin were incubated with 0 or 100 nM PRC2 respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 3 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H3K27me1 antibody . Mononucleosomes H3.1 were used as a control. The Western Blot result shows that only the H3K27 of mononucleosomes H3.1 can be methylated by PRC2.)
SDS-PAGE (Recombinant Mononucleosomes H3.1 (K27M) - biotin 12.5% SDS-PAGE gel, stained with Coomassie Blue MW: 108 kDa Purity: >95%)
Application Data (Recombinant Mononucleosomes H3.1 (K27M) - biotin DNA gel Recombinant Mononucleosomes H3.1 (K27M) - biotin were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA which was used for assembly of nucleosomes. Lane 3: Intact mononucleosomes H3.1 (K27M) - biotin. Intact mononucleosomes H3.1 (K27M) - biotin migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrapped histone octamers to form nucleosomes.)
SDS-PAGE (Streptavidin pull down assay for Recombinant Mononucleosomes H3.2 – biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1hr at 4 degree C. Streptavidin beads were washed 3 times with 1 ml binding buffer. Then the beads were added 60 ul 2×SDS loading buffer and boiled for 10 min at 95 degree C. 2.4 ul samples were loaded and run on a 12.5% SDS-PAGE gel and stained by Commassie blue. The SDS-PAGE gel result showed that almost all of biotinylated mononucleosomes were pulled down by streptavidin beads.)
Application Data (Recombinant Mononucleosomes H3.2- biotin, DNA gel Recombinant Mononucleosomes H3.2 - biotin were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: Free 601 DNA which was used for assembly of nucleosome. Lane 3: Intact mononucleosomes H3.2 - biotin. Intact mononucleosomes H3.2- biotin migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrapped histone octamers to form nucleosomes.)
SDS-PAGE (Recombinant Mononucleosomes H3.2 – biotin 12.5% SDS-PAGE gel with Coomassie blue staining MW: 108 kDa Purity: >92%)
SDS-PAGE (Streptavidin pull down assay for Recombinant Mononucleosomes H3.3 (K9I) – biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1 hr at 4 degree C. Streptavidin beads were washed 3 times with 1 ml binding buffer. The beads were added to 60 ul 2×SDS loading buffer and boiled for 10 min at 95 degree C. 2.4 ul samples were loaded and run on a 12.5% SDS-PAGE gel stained with Coomassie blue. The SDS-PAGE gel shows that almost all of the biotinylated mononucleosomes were pulled down by streptavidin beads.)
Application Data (Recombinant Mononucleosomes H3.3 (K9I) - biotin DNA gel Recombinant Mononucleosomes H3.3 (K9I) - biotin were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: Free 601 DNA which was used for assembly of nucleosome. Lane 3: Intact mononucleosomes H3.3 (K9M) - biotin. Intact mononucleosomes H3.3 (K9I) - biotin migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrapped histone octamers to form nucleosomes.)
SDS-PAGE (Recombinant Mononucleosomes H3.3 (K9I) – biotin 12.5% SDS-PAGE with Coomassie blue staining MW: 108 kDa Purity: >92%)
WB (Western Blot) (Histone methyltransferase activity assay comparing recombinant polynucleosomes and histone octamers as substrates. 2 ug Recombinant Polynucleosomes H3.3 (G34R) were incubated with DOT1L in reaction buffer for 3 hours at room temperature. Western Blot was used for detecting the generation of reaction products (H3K79me1). DOT1L only and polynucleosomes only were used as negative control. The Western Blot result shows that polynucleosomes are more suitable substrate for DOT1L than histone octamers.)
Application Data (DNA Gel-shift assay for Recombinant Polynucleosomes H3.3 (G34R). Polynucleosomes H3.3 (G34R) and free plasmid DNA were run on a 1% agarose gel and stained with ethidium bromide. Intact polynucleosomes migrate much higher than free DNA, thus the DNA resolves at a higher molecular weight when nucleosome-bound.)
SDS-PAGE (Recombinant Polynucleosomes H3.3 (G34R) Polynucleosomes were run on a 12% SDS-PAGE gel and stained with Coomassie Blue. The purity of the nucleosomes H3.3 (G34L) is > 95%.)
HIV-1 gp120 (pCAAN5342) (Clade B) protein (amino acid 34-518)
Applications
Western Blot
Purity
> 95% (SDS-PAGE)
Pricing
What Are Recombinant Proteins?
Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.
The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.
Common Applications of Recombinant Proteins
Studying protein-protein and protein-DNA interactions
Functional assays to study biological pathways
Standard curves in ELISA and other quantitative assays
Use as antigens for antibody production
Development and screening of therapeutic drugs
Biomarker discovery and validation
Cell signaling and immune response studies
Vaccine research and development
Key Features of AAA Biotech’s Recombinant Proteins
High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.
Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.
Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.
Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.
Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.
Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.
Flexible Quantities: Available in various pack sizes to suit different experimental needs.
Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.
Why Buy Recombinant Proteins from AAA Biotech?
At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.
FAQ
1. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product.
2. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
3. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.
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