At AAA Biotech, we offer a comprehensive selection of high-quality recombinant proteins for use in a wide range of research areas, including immunology, neuroscience, stem cell research, cancer research and more. No matter whether you need recombinant proteins for cell expansion, polarization, differentiation, or cell processing applications, we have got you covered.
Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.
WB (Western Blot) (Western Blot analysis for Recombinant Mononucleosomes H3K9me3 (EPL) - biotin. Unmodified nucleosomes (Lane 1) and Recombinant Mononucleosomes H3K9me3 (EPL) - biotinylated (Lane 2) were detected with anti-H3K9me3 antibody and anti-H4 antibody, respectively. H4 was detected as loading control. Only Recombinant Mononucleosomes H3K9me3 (EPL) - biotinylated can be detected by anti-H3K9me3 antibody.)
Application Data (Recombinant Mononucleosomes H3K9me3 (EPL) - biotin, DNA gel. Recombinant Mononucleosomes H3K9me3 (EPL) - biotinylated were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: Intact monnucleosomes H3K9me3. Lane 3: 601 DNA. Intact mononucleosomes H3K9me3 migrated much higher than free 601 DNA. The agarose gel result shows almost all of 601 DNA wrap histone octamers to form nucleosomes.)
SDS-PAGE (Streptavidin pull-down for Recombinant Mononucleosomes H3K9me3 (EPL) - biotin. Recombinant Mononucleosomes H3K9me3 (EPL) - biotin were pulled down by streptavidin beads. Input nucleosomes (Lane 2) and the nucleosomes (Lane 3) pulled down by streptavidin were run on a 13% SDS-PAGE gel and stained with Commassie blue. The SDS-PAGE gel result shows that almost all of biotinylated mononucleosomes H3K9me3 are pulled down by streptavidin beads. * indicates streptavidin.)
WB (Western Blot) (Histone methyltransferase activity assay comparing recombinant polynucleosomes and histone octamers as substrates. 2 ug Recombinant Polynucleosomes H3.3 (G34V) were incubated with DOT1L in reaction buffer for 3 hours at room temperature. Western Blot was used for detecting the generation of reaction products (H3K79me1). DOT1L only and polynucleosomes only were used as negative control. The Western Blot result shows that polynucleosomes are more suitable substrate for DOT1L than histone octamers.)
Application Data (DNA Gel-shift assay for Recombinant Polynucleosomes H3.3 (G34V). Polynucleosomes H3.3 (G34V) and free plasmid DNA were run on a 1% agarose gel and stained with ethidium bromide. Intact polynucleosomes migrate much higher than free DNA, thus the DNA resolves at a higher molecular weight when nucleosome-bound.)
SDS-PAGE (Recombinant Polynucleosomes H3.3 (G34V) Polynucleosomes were run on a 12% SDS-PAGE gel and stained with Coomassie Blue.)
WB (Western Blot) (Western blot assay for Recombinant Mononucleosomes H3.1 (K9M) 0.5 ug Recombinant Histone H3.1 (K9M) which was used for assembly of nucleosomes was incubated with 0 or 5 nM G9A respectively in reaction buffer containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 uM SAM for 3 hr at room temperature. Half of the reactions were run on a 12.5% SDS-PAGE gel and detected with H3K9me2 antibody . Histone H3.1 was used as a control. The Western Blot result shows that only the K9 of histone H3.1 can be methylated by G9A.)
SDS-PAGE (Recombinant Mononucleosomes H3.1 (K9M) 12.5% SDS-PAGE gel stained with Coomassie Blue MW: 108 kDa Purity: >95%)
Application Data (Recombinant Mononucleosomes H3.1 (K9M) DNA gel Recombinant Mononucleosomes H3.1 (K9M) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA which was used for assembly of nucleosomes. Lane 3: Intact mononucleosomes H3.1 (K9M). Intact mononucleosomes H3.1 (K9M) migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrapped histone octamers to form nucleosomes.)
SDS-PAGE (Streptavidin pull down assay for Recombinant Mononucleosomes H3.3 (K18I) – biotin 24 ug biotinylated mononucleosomes were incubated with 10 ul streptavidin beads for 1hr at 4 degree C. Streptavidin beads were washed 3 times with 1 ml binding buffer. Then the beads were added to 60 &mixeo;l 2×SDS loading buffer and boiled for 10 min at 95°&C. 2.4 micro;l samples were loaded and run on a 12.5% SDS-PAGE gel and stained by Commassie blue. The SDS-PAGE gel shows that almost all of biotinylated mononucleosomes were pulled down by streptavidin beads.)
Application Data (Recombinant Mononucleosomes H3.3 (K18I) - biotin DNA gel Recombinant Mononucleosomes H3.3 (K18I) - biotin were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: Free 601 DNA which was used for assembly of nucleosome. Lane 3: Intact mononucleosomes H3.3 (K18I) - biotin. Intact mononucleosomes H3.3 (K18I) - biotin migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrapped histone octamers to form nucleosomes.)
SDS-PAGE (Recombinant Mononucleosomes H3.3 (K18I) – biotin 12.5% SDS-PAGE with Coomassie blue staining MW: 108 kDa Purity: >92%)
Application Data (Recombinant Mononucleosomes H3.3 (K18I) DNA gel Recombinant Mononucleosomes H3.3 (K18I) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA which was used for assembly of nucleosome. Lane 3: Intact mononucleosomes H3.3 (K18I). Intact mononucleosomes H3.3 (K18I) migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrapped histone octamers to form nucleosomes.)
Application Data (Recombinant Mononucleosomes H3.3 (K4I) DNA gel Recombinant Mononucleosomes H3.3 (K4I) were run on a 2% agarose gel and stained with ethidium bromide. Lane 1: DNA marker. Lane 2: 601 DNA which was used for assembly of nucleosome. Lane 3: Intact mononucleosomes H3.3 (K4I). Intact mononucleosomes H3.3 (K4I) migrated much higher than free 601 DNA. The agarose gel shows that almost all of 601 DNA wrapped histone octamers to form nucleosomes.)
Application Data (HTRF assay for BRPF1 (627-746) activity 3 uM histone H3K27ac peptide was incubated with different concentrations of BRPF1 (627-746) protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (HTRF assay for BRPF1 (627-746) activity 3 uM histone H3K27ac peptide was incubated with different concentrations of BRPF1 (627-746) protein in 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.1% BSA for 1 hour, then 10 ul DYKDDDDK antibody and SA-XL665 mixture (each 1:100 dilution in the reaction buffer) was added to each reaction system and incubated for 30 min. All operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant BRPF1 (627-746) protein gel. BRPF1 (627-746) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. M: 17.4 kDa Purity: > 90%)
ELISA (Biotinylated Human Siglec-10 (R119A) Protein ELISA DataImmobilized Anti-Siglec-10 Antibody, hFc Tag at 5?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human Siglec-10 (R119A) , His Tag with the EC50 of 61.7ng/ml determined by ELISA.)
Application Data (Biotinylated Human Siglec-10 (R119A) Protein SEC-HPLCThe purity of Biotinylated Human Siglec-10 (R119A) is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human Siglec-10 (R119A) Protein Tris-Bis PAGEBiotinylated Human Siglec-10 (R119A) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Application Data (Biotinylated Human NKG2C&CD94 Protein SEC-HPLCThe purity of Biotinylated Human NKG2C&CD94 is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human NKG2C&CD94 Protein Tris-Bis PAGEBiotinylated Human NKG2C&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Application Data (Biotinylated Human Fc gamma RI/CD64 Protein SPR DataBiotinylated Human Fc gamma RI, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Trastuzumab with an affinity constant of 0.91 nM as determined in SPR assay (Biacore T200).)
Application Data (Biotinylated Human Fc gamma RI/CD64 Protein SEC-HPLCThe purity of Biotinylated Human Fc gamma RI is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human Fc gamma RI/CD64 Protein Tris-Bis PAGEBiotinylated Human Fc gamma RI on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Application Data (Biotinylated Mouse CX3CL1/Fractalkine Protein SEC-HPLCThe purity of Biotinylated Mouse CX3CL1 is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Mouse CX3CL1/Fractalkine Protein Tris-Bis PAGEBiotinylated Mouse CX3CL1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
ELISA (Biotinylated Human EpCAM/TROP1 Protein ELISA DataImmobilized Biotinylated Human EpCAM, His Tag at 0.2?g/ml (100ul/Well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 15.6ng/ml determined by ELISA.)
Application Data (Biotinylated Human EpCAM/TROP1 Protein SEC-HPLCThe purity of Biotinylated Human EpCAM is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human EpCAM/TROP1 Protein Tris-Bis PAGEBiotinylated Human EpCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..)
ELISA (Biotinylated Human PD-L1/B7-H1 Protein ELISA DataImmobilized Anti-PD-L1 Antibody, hFc Tag at 0.5?g/ml (100?l/Well) on the plate. Dose response curve for Biotinylated Human PD-L1, His Tag with the EC50 of 2.8ng/ml determined by ELISA.)
Application Data (Biotinylated Human PD-L1/B7-H1 Protein SEC-HPLCThe purity of Biotinylated Human PD-L1 is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human PD-L1/B7-H1 Protein Tris-Bis PAGEBiotinylated Human PD-L1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
ELISA (Biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein ELISA DataImmobilized Human Latent TGF beta 1, His Tag at 2?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 1.2?g/ml determined by ELISA.)
Application Data (Biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein SEC-HPLCThe purity of Biotinylated Human ITGAV&ITGB6 is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human Integrin alpha V beta 6 (ITGAV&ITGB6) Heterodimer Protein Tris-Bis PAGEBiotinylated Human ITGAV&ITGB6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Application Data (Biotinylated Mouse PSMA/FOLH1 Protein (Primary Amine Labeling) SEC-HPLCThe purity of Biotinylated Mouse PSMA is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Mouse PSMA/FOLH1 Protein (Primary Amine Labeling) Tris-Bis PAGEBiotinylated Mouse PSMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
ELISA (Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) ELISA DataImmobilized Anti-CD3 Antibody, mFc Tag at 5?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3D, His Tag with the EC50 of 0.14?g/ml determined by ELISA.)
Application Data (Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) SEC-HPLCThe purity of Biotinylated Human CD3E&CD3D is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) Tris-Bis PAGEBiotinylated Human CD3E&CD3D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
ELISA (Biotinylated Human CD200 R1/CRTR2 Protein ELISA DataImmobilized Human CD200, hFc Tag at 5?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human CD200 R1, His Tag with the EC50 of 0.64?g/ml determined by ELISA.)
Application Data (Biotinylated Human CD200 R1/CRTR2 Protein SEC-HPLCThe purity of Biotinylated Human CD200 R1 is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human CD200 R1/CRTR2 Protein Tris-Bis PAGEBiotinylated Human CD200 R1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
ELISA (Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein ELISA DataImmobilized Mouse ITGAV&ITGB6, His Tag at 1?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Mouse GARP&Latent TGF beta 1 Complex, His Tag with the EC50 of 0.12?g/ml determined by ELISA.)
Application Data (Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein SEC-HPLCThe purity of Biotinylated Mouse GARP&Latent TGF beta 1 Complex is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Mouse GARP&Latent TGF beta 1 Complex Protein Tris-Bis PAGEBiotinylated Mouse GARP&Latent TGF beta 1 Complex on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
ELISA (Biotinylated Human GDF15 Protein (Primary Amine Labeling) ELISA DataImmobilized Human GFRAL, His Tag at 1?g/ml (100?l/Well) on the plate. Dose response curve for Biotinylated Human GDF15, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.)
Application Data (Biotinylated Human GDF15 Protein (Primary Amine Labeling) SEC-HPLCThe purity of Biotinylated Human GDF15 is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human GDF15 Protein (Primary Amine Labeling) Tris-Bis PAGEBiotinylated Human GDF15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
ELISA (Biotinylated Human CD4/LEU3 Protein ELISA DataImmobilized Biotinylated Human CD4, His Tag at 0.5?g/ml (100?l/Well) on the plate. Dose response curve for Anti-CD4 Antibody, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA.)
Application Data (Biotinylated Human CD4/LEU3 Protein SEC-HPLCThe purity of Biotinylated Human CD4 is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human CD4/LEU3 Protein Tris-Bis PAGEBiotinylated Human CD4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
ELISA (Biotinylated Human LAP (TGF beta 1) Protein ELISA DataImmobilized Human ITGAV&ITGB6, His Tag at 1?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human LAP (TGF beta 1) , His Tag with the EC50 of 0.86?g/ml determined by ELISA.)
ELISA (Biotinylated Human LAP (TGF beta 1) Protein ELISA DataImmobilized Biotinylated Human LAP (TGF beta 1) , His Tag at 0.5?g/ml (100?l/Well) on the plate. Dose response curve for Human TGF-beta RII, hFc Tag with the EC50 of 23.8ng/ml determined by ELISA.)
Application Data (Biotinylated Human LAP (TGF beta 1) Protein SEC-HPLCThe purity of Biotinylated Human LAP (TGF beta 1) is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human LAP (TGF beta 1) Protein Tris-Bis PAGEBiotinylated Human LAP (TGF beta 1) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
ELISA (Biotinylated Human LILRB4/CD85k/ILT3 Protein ELISA DataImmobilized Anti-LILRB4 Antibody, hFc Tag at 1?g/ml (100?l/well) on the plate. Dose response curve for Biotinylated Human LILRB4, His Tag with the EC50 of 7ng/ml determined by ELISA.)
Application Data (Biotinylated Human LILRB4/CD85k/ILT3 Protein SEC-HPLCThe purity of Biotinylated Human LILRB4 is greater than 95% as determined by SEC-HPLC.)
Tris-Bis-PAGE (Biotinylated Human LILRB4/CD85k/ILT3 Protein Tris-Bis PAGEBiotinylated Human LILRB4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Biotinylated Human LILRB4/CD85k/ILT3 Protein Recombinant
Purity
>95%, as determined by SDS-PAGE and HPLC
Pricing
What Are Recombinant Proteins?
Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.
The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.
Common Applications of Recombinant Proteins
Studying protein-protein and protein-DNA interactions
Functional assays to study biological pathways
Standard curves in ELISA and other quantitative assays
Use as antigens for antibody production
Development and screening of therapeutic drugs
Biomarker discovery and validation
Cell signaling and immune response studies
Vaccine research and development
Key Features of AAA Biotech’s Recombinant Proteins
High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.
Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.
Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.
Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.
Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.
Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.
Flexible Quantities: Available in various pack sizes to suit different experimental needs.
Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.
Why Buy Recombinant Proteins from AAA Biotech?
At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.
FAQ
1. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product.
2. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
3. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.
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