At AAA Biotech, we offer a comprehensive selection of high-quality recombinant proteins for use in a wide range of research areas, including immunology, neuroscience, stem cell research, cancer research and more. No matter whether you need recombinant proteins for cell expansion, polarization, differentiation, or cell processing applications, we have got you covered.
Our recombinant proteins undergo rigorous quality testing. So, you can rely on AAA Biotech for high-quality recombinant proteins to support your research. Explore our catalog to find the right protein for your research needs.
Application Data (HTRF assay for JAK1 (866-1154) activity 1 uM TK substrate was incubated with different concentrations of JAK1 (866-1154) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM MnCl2, 1 mM DTT, and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant JAK1 (866-1154) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 34.2 kDa Purity: >95%)
Application Data (HTRF assay for MER (528-999) activity 1 uM TK substrate was incubated with different concentrations of JAK1 (866-1154) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM MnCl2, 1 mM DTT, and 100 uM ATP for 1 hour. Then 10 ul detection reagents containing TK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant MER(528-999) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 54 kDa Purity: >50%)
Application Data (This protein does not contain any Trp residues. Experience shows that this could result in more than 10% error in the computed extinction coefficient. Therefore, we have meassured the protein concentration by using the colorimetric assay based on the interaction between Coomassie brilliant blue and the arginine and aromatic residues (Bradford Method) and its maximum absorption shifts from 470 nm to 595 nm. The standard curve was performed with the protein BSA. 40 ul of the protein were analysed.DO595 = 0.66)
SDS-PAGE (Figure 1. SDS-PAGE analysis (17%) of 3 ul of recombinant allergen. Purity is >95% as determined by gel electrophoresis.)
SDS-PAGE (Figure 1. SDS-PAGE analysis (12%) of 10 ul of recombinant allergen. Purity is >95% as determined by gel electrophoresis. Bands slightly smaller correspond to the same protein.)
Application Data (Figure 3. Binding ability measured in a functional ELISA. Immobilized human ACE2(19-740) protein (Fc tag) can bind to SARS-CoV Spike Protein S1 (His tag).)
Application Data (Figure 2. Binding ability measured in a functional ELISA. Anti-2019-nCoV Spike Protein binds to immobilized SARS-CoV Spike Protein S1 (His tag).)
SDS-PAGE (Figure 1. SDS-PAGE gel imageThe purity of SARS-CoV Spike Protein S1 was determined to be >90% by densitometry, approx. MW 125 kDa.)
SDS-PAGE (Tau Proteins: Representation of seven Tau proteins, from top to bottom: Tau-441, Tau-410, Tau-412, Tau-381, Tau-383, Tau-352 and Tau-316 (Tau Protein Marker).)
SDS-PAGE (Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Application Data (Recombinant SMARCA4 / BRG1 (1448-1569) HTRF activity assay. 3 uM H3K14ac peptide was incubated with different concentrations of SMARCA4 / BRG1 (1448-1569) protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour, then 10 ul Anti-GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data (Recombinant SMARCA4 / BRG1 (1448-1569) HTRF activity assay. 3 uM H3K14ac peptide was incubated with different concentrations of SMARCA4 / BRG1 (1448-1569) protein in a 10 ul binding system containing 50 mM HEPES-NaOH pH 7.4, and 0.1% BSA for 1 hour, then 10 ul Anti-GST antibody and SA-XL665 mixture (each 1:100 dilution in Binding Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE (Recombinant SMARCA4 / BRG1 (1448-1569), GST-Tag protein gel. SMARCA4 / BRG1 (1448-1569), GST-Tag protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie blue.)
WB (Western Blot) (Recombinant KAT7 protein activity assay 0.5 ug Histone H4 was incubated with 0 ug (-), 0.4 ug (+) KAT7 in 20 ul reaction system for 2 hours at room temperature. Half of each reaction was run on a 13% SDS-PAGE gel, and products were detected by Western blot. H4ac (pan-acetyl) antibody was used to recognize acetylated histone H4.)
SDS-PAGE (Recombinant KAT7 protein gel. Recombinant KAT7 was run on a 10% SDS-PAGE gel and stained with Coomassie blue. Mw: 67 kDa Purity: >85%)
Application Data (MTase-Glo assay for METTL3 / METTL14 Complex m6A methyltransferase activity 1 ?M Substrate RNA (UAGAGGACCAGUCGGACCAGUCGGACCGAU) and 1 ?M SAM was incubated with different concentrations of METTL3 / METTL14 Complex in an 8 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, and 1 mM TCEP at room temperature for 1 hour. 5xMTase-Glo Reagent was added to the products and incubated for 30 min, then MTase-Glo Detection was added and luminescence were read after another 30 min incubation. SAH standard curve (0-1 uM) was performed following the same protocol.)
Application Data (MTase-Glo assay for METTL3 / METTL14 Complex m6A methyltransferase activity 1 ?M Substrate RNA (UAGAGGACCAGUCGGACCAGUCGGACCGAU) and 1 ?M SAM was incubated with different concentrations of METTL3 / METTL14 Complex in an 8 ul reaction system containing 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, and 1 mM TCEP at room temperature for 1 hour. 5xMTase-Glo Reagent was added to the products and incubated for 30 min, then MTase-Glo Detection was added and luminescence were read after another 30 min incubation. SAH standard curve (0-1 uM) was performed following the same protocol.)
SDS-PAGE (Recombinant METTL3 / METTL14 complex, protein gel. Recombinant METTL3 / METTL14 complex was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: METTL3: 64.5 kDa, MW: METTL14, N-DYKDDDDK: 53.3 kDa. Purity: >85%)
Application Data (HTRF Assay for Recombinant PRC2 EZH2(Y641F) complex activity. 1 ?M histone H3K27me2 (21-44aa.) peptide was incubated with different concentrations of Recombinant PRC2 EZH2 (Y641F) Complex in reaction buffer including 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 ?M SAM for 3 hours at room temperature. Then 10 ?l anti-H3K27me3 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000.)
Application Data (HTRF Assay for Recombinant PRC2 EZH2(Y641F) complex activity. 1 ?M histone H3K27me2 (21-44aa.) peptide was incubated with different concentrations of Recombinant PRC2 EZH2 (Y641F) Complex in reaction buffer including 50 mM Tris-HCl pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP and 50 ?M SAM for 3 hours at room temperature. Then 10 ?l anti-H3K27me3 antibody and SA-XL665 mixture (1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min at room temperature. HTRF assay was used for detection. Fluorenscence intensity at 620 nm and 665 nm were measured respectively. The final HTRF signal was obtained by formula: HTRF signal = F665/F620*10000.)
SDS-PAGE (Recombinant PRC2 EZH2 (Y641F) complex protein gel. PRC2 EZH2(Y641F) complex was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW of components: EZH2 (Y641F): 87 kDa. SUZ12: 83 kDa. EED: 50.2 kDa. RbAp46: 47.8 kDa. RbAp48: 47.7 kDa. Purity: 85%)
Application Data (Recombinant DNMT3A protein activity assay Recombinant DNMT3A protein activity was measured using DNMT Activity / Inhibition Assay, for 1 hour at 37 degree C)
SDS-PAGE (Recombinant DNMT3A protein gel. Recombinant DNMT3A protein was run on an 8% SDS-PAGE gel and stained with Coomassie blue.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized IGFBP7 at 20 ug/ml (100 ul/well) can bind biotinylated human IGF2-nusa. The EC50 of biotinylated human IGF2-nusa is 0.57 ug/ml.)
Application Data (Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(Cat: 10108-H05H) at 2ug/mL (100uL/well) can bind SARS-CoV-2 Spike RBD (L452Q, F490S) Protein (His Tag)(Cat:40592-V08H113), the EC50 of SARS-CoV-2 Spike RBD (L452Q, F490S) Protein (His Tag)(Cat:40592-V08H113) is 8-48ng/mL.)
Recombinant proteins are purified laboratory reagents produced through genetic engineering. A specific gene of interest is inserted into a host organism, such as mammalian, bacterial, yeast, or insect cells, which then expresses the protein in a controlled environment.
The recombinant process utilized to generate the recombinant proteins in our catalog provides precise control over sequence modifications, expression levels, and large-scale production tailored to experimental needs. These recombinants are widely used in research to investigate protein-protein interactions, enzyme activities, receptor-ligand binding, and cellular responses. Additionally, recombinant proteins serve as standards or controls in immunostaining assays and support cell growth and differentiation in culture systems, particularly in immunology, oncology, and structural biology studies.
Common Applications of Recombinant Proteins
Studying protein-protein and protein-DNA interactions
Functional assays to study biological pathways
Standard curves in ELISA and other quantitative assays
Use as antigens for antibody production
Development and screening of therapeutic drugs
Biomarker discovery and validation
Cell signaling and immune response studies
Vaccine research and development
Key Features of AAA Biotech’s Recombinant Proteins
High Purity: Most proteins are purified to ≥95% using affinity chromatography and other validated techniques.
Biological Activity: If functional activity is tested and confirmed for a given protein, it will be noted directly on the product page.
Multiple Expression Systems: Available in E. coli, HEK293, CHO, yeast, and insect cells to match your assay requirements.
Custom Tags Available: His-tag, GST, FLAG, and Fc fusion options for easy purification and detection.
Wide Range of Targets: Cytokines, growth factors, enzymes, receptors, signaling proteins, and more.
Validated Consistency: Lot-to-lot consistency ensured through rigorous QC protocols.
Flexible Quantities: Available in various pack sizes to suit different experimental needs.
Ready-to-Use Format: Lyophilized or liquid formulations that are easy to reconstitute and use.
Why Buy Recombinant Proteins from AAA Biotech?
At AAA Biotech, we are committed to supporting the research community with recombinant proteins that offer exceptional performance and reliability. Our proteins are produced using industry-standard methods and are validated to meet the needs of academic, pharmaceutical, and biotechnology laboratories.
With competitive pricing, global shipping, and dedicated technical support, AAA Biotech makes it simple and convenient to source the high-quality recombinant proteins your work depends on.
FAQ
1. How are AAA Biotech recombinant proteins validated?
Each batch undergoes stringent quality control checks, including SDS-PAGE analysis, endotoxin testing (for select products), and activity assaying (for select products). Certificates of Analysis are provided with every product.
2. Are your proteins suitable for therapeutic development or only research?
AAA Biotech recombinant proteins are strictly for research-use only and are not intended for diagnostic or therapeutic purposes in humans or animals.
3. What types of expression systems do you use for recombinant protein production?
The production labs use a variety of expression platforms, including bacterial (E. coli), yeast, insect (baculovirus), and mammalian (HEK293, CHO) systems. The expression system used depends on the complexity and intended function/use of the protein.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.