Rabbit anti-Human VISTA Polyclonal Antibody | anti-VISTA antibody

VISTA Antibody

Cat. Num. AAA40885

• Gene Names: VSIR; B7H5; GI24; B7-H5; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot, ELISA
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: VISTA, Antibody; VISTA Antibody; VISTA molecule; VSIR; B7-H5; B7H5; GI24; PP2135; SISP1; DD1alpha; VISTA; C10orf54; chromosome 10 open reading frame 54; PD-1H; V-set immunoregulatory receptor; V-Type Immunoglobulin Domain-Containing Suppressor Of T-Cell Activation; Chromosome 10 Open Reading Frame 54; anti-VISTA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid; Supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 311

• Applicable Applications for anti-VISTA antibody: IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: The immunogen is located within the last 60 amino acids of VISTA.
• Conjugate: Unconjugated
• Homology: Predicted Species reactivity based on immunogen sequence: Mouse: (93%).; ; VISTA antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human VISTA.
• Preparation and Storage: VISTA antibody can be stored at 4°C for three months and -20°C stable for up to one year. As with all antibodies care should be taken to avoid repeated freeeze/thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

ICC (Immunocytochemistry)

(Immunocytochemistry of VISTA in overexpressing HEK293 cells with VISTA antibody at 1 μg/ml.)

IF (Immunofluorescence)

(Immunofluorescence of VISTA in human tonsil tissue with B7-H5 antibody at 20 μg/ml.)

IHC (Immunohistochemistry)

(Immunohistochemistry of VISTA in human tonsil tissue with B7-H5 antibody at 2 μg/ml.)

Related Product Information for anti-VISTA antibody

The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5).

Product Categories/Family for anti-VISTA antibody

Immunology

References

Mayya V., et al. Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. 2009, Sci. Signal. 2:RA46-RA46. Sakr M.A., et al.,GI24 enhances tumor invasiveness by regulating cell surface membrane-type 1 matrix metalloproteinase. 2010, Cancer Sci. 101:2368-2374.

NCBI and Uniprot Product Information

• NCBI GI #: 62339432
• NCBI GeneID: 64115
• NCBI Accession #: NP_071436
• NCBI GenBank Nucleotide #: NM_022153.1
• UniProt Accession #: Q9H7M9; Q6UXF3; Q8WUG3; Q8WYZ8; A1L0X9; A4ZYV1; A8MVH5
• NCBI Official Full Name: V-type immunoglobulin domain-containing suppressor of T-cell activation
• NCBI Official Synonym Full Names: V-set immunoregulatory receptor
• NCBI Official Symbol: VSIR
• NCBI Official Synonym Symbols: B7H5; GI24; B7-H5; PD-1H; SISP1; VISTA; PP2135; C10orf54; DD1alpha
• NCBI Protein Information: V-type immunoglobulin domain-containing suppressor of T-cell activation
• UniProt Protein Name: V-type immunoglobulin domain-containing suppressor of T-cell activation
• UniProt Gene Name: VSIR
• UniProt Synonym Gene Names: Sisp-1