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FCM/FACS (Flow Cytometry)
(Human B7H5 CHO-K cells were stained with Anti-B7-H5 / VISTA Reference Antibody (onvatilimab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC328=0.9471 ug/mL)
B7-H5/VISTA recombinant antibody
Anti-B7-H5/VISTA Reference Antibody (onvatilimab)
Gene Names
C10orf54; B7H5; GI24; B7-H5; SISP1; VISTA; PP2135; DD1alpha
Reactivity
Human, Mouse, Cynomolgus
Applications
Functional Assay, Flow Cytometry, Functional Assay, ELISA
Synonyms
B7-H5/VISTA, Recombinant Antibody; Anti-B7-H5/VISTA Reference Antibody (onvatilimab); B7-H5/VISTA recombinant antibody
Host
CHO Cell
Reactivity
Human, Mouse, Cynomolgus
Clonality
Monoclonal
Isotype
IgG1
Specificity
B7-H5/VISTA
Form/Format
Purified monoclonal antibody supplied in PBS, pH6.0, without preservative.This antibody is purified through a protein A column.
Concentration
1mg/ml (varies by lot)
Applicable Applications for B7-H5/VISTA recombinant antibody
FA (Functional Assay), FCM/FACS (Flow Cytometry), ELISA
Endotoxin
< 0.001EU/ug, determined by LAL method.
Conjugation
Unconjugated
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store at -80 degree C for 2 years under sterile conditions; -20 degree C for 1 year under sterile conditions; Avoid repeated freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(Human B7H5 CHO-K cells were stained with Anti-B7-H5 / VISTA Reference Antibody (onvatilimab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC328=0.9471 ug/mL)
FCM/FACS (Flow Cytometry)
(Human B7H5 CHO-K cells were stained with Anti-B7-H5 / VISTA Reference Antibody (onvatilimab) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC328=0.9471 ug/mL)
Application Data
(Immobilized human B7-H5 / VISTA His at 2 ug/mL can bind Anti-B7-H5 / VISTA Reference Antibody (onvatilimab), EC50=0.01513ug/mL)
Application Data
(Immobilized human B7-H5 / VISTA His at 2 ug/mL can bind Anti-B7-H5 / VISTA Reference Antibody (onvatilimab), EC50=0.01513ug/mL)
Application Data
(The purity of Anti-B7-H5 / VISTA Reference Antibody (onvatilimab)is more than 98.11% ,determined by SEC-HPLC.)
Application Data
(The purity of Anti-B7-H5 / VISTA Reference Antibody (onvatilimab)is more than 98.11% ,determined by SEC-HPLC.)
SDS-PAGE
(Anti-B7-H5 / VISTA Reference Antibody (onvatilimab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%)
SDS-PAGE
(Anti-B7-H5 / VISTA Reference Antibody (onvatilimab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%)
Product Categories/Family for B7-H5/VISTA recombinant antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,908 Da
NCBI Official Full Name
platelet receptor Gi24
NCBI Official Synonym Full Names
chromosome 10 open reading frame 54
NCBI Official Symbol
C10orf54
NCBI Official Synonym Symbols
B7H5; GI24; B7-H5; SISP1; VISTA; PP2135; DD1alpha
NCBI Protein Information
platelet receptor Gi24
UniProt Protein Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
UniProt Gene Name
C10orf54
UniProt Synonym Gene Names
; Sisp-1
UniProt Entry Name
VISTA_HUMAN
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Product Notes
The B7-H5/VISTA c10orf54 (Catalog #AAA296963) is a Recombinant Antibody produced from CHO Cell and is intended for research purposes only. The product is available for immediate purchase. The Anti-B7-H5/VISTA Reference Antibody (onvatilimab) reacts with Human, Mouse, Cynomolgus and may cross-react with other species as described in the data sheet. AAA Biotech's B7-H5/VISTA can be used in a range of immunoassay formats including, but not limited to, FA (Functional Assay), FCM/FACS (Flow Cytometry), ELISA. Researchers should empirically determine the suitability of the B7-H5/VISTA c10orf54 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B7-H5/VISTA, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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