WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells using Parkin antibody. The lane on the left is treated with synthesized peptide.)
Rabbit Parkin Polyclonal Antibody | anti-Parkin antibody
Parkin Antibody
Gene Names
PARK2; PDJ; PRKN; AR-JP; LPRS2
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
Parkin, Antibody; Parkin Antibody; PARK2; PRKN; PRKN2; Parkinson disease protein 2; Parkinson juvenile disease protein 2; anti-Parkin antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous levels of total Parkin protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
465
Applicable Applications for anti-Parkin antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Peptide
Immunogen Description
Synthesized peptide derived from human Parkin antibody.
Target Name
Parkin
Preparation and Storage
Store at -20 degree C
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells using Parkin antibody. The lane on the left is treated with synthesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Jurkat cells using Parkin antibody. The lane on the left is treated with synthesized peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Jukat cells, using Parkin antibody.)
WB (Western Blot)
(Western blot analysis of extracts from Jukat cells, using Parkin antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human brain tissue using Parkin antibody.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human brain tissue using Parkin antibody.)
Related Product Information for anti-Parkin antibody
Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins, such as BCL2, SYT11, CCNE1, GPR37, STUB1, a 22 kDa O-linked glycosylated isoform ofSNCAIP, SEPT5, ZNF746 and AIMP2. Mediates monoubiquitination as well as 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context. Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation. Mediates 'Lys-63'-linked polyubiquitination of SNCAIP, possibly playing a role in Lewy-body formation. Mediates monoubiquitination of BCL2, thereby acting as a positive regulator of autophagy. Promotes the autophagic degradation of dysfunctional depolarized mitochondria (mitophagy), potentially by the ubiquitination of mitochondrial proteins. Mediates 'Lys-48'-linked polyubiquitination of ZNF746, followed by degradation of ZNF746 by the proteasome; possibly playing a role in the regulation of neuron death. Limits the production of reactive oxygen species (ROS). Regulates cyclin-E during neuronal apoptosis. In collaboration with CHPF isoform 2, may enhance cell viability and protect cells from oxidative stress. Independently of its ubiquitin ligase activity, protects from apoptosis by the transcriptional repression of p53/TP53. May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity. May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. May represent a tumor suppressor gene.Fang Yang, J. Biol. Chem., Apr 2005; 280: 17154 - 17162. Yukiko Kuroda, Hum. Mol. Genet., Mar 2006; 15: 883 - 895. Iris H. Henn, J. Neurosci., Feb 2007; 27: 1868 - 1878. Monica Joch, Mol. Biol. Cell, Jun 2007; 10.1091/mbc.E05-11-1027.
Product Categories/Family for anti-Parkin antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,413 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase parkin isoform 1
NCBI Official Synonym Full Names
parkin RBR E3 ubiquitin protein ligase
NCBI Official Symbol
PARK2
NCBI Official Synonym Symbols
PDJ; PRKN; AR-JP; LPRS2
NCBI Protein Information
E3 ubiquitin-protein ligase parkin
UniProt Protein Name
E3 ubiquitin-protein ligase parkin
UniProt Gene Name
PARK2
UniProt Synonym Gene Names
PRKN; Parkin; Parkinson disease protein 2
UniProt Entry Name
PRKN2_HUMAN
Similar Products
Product Notes
The Parkin park2 (Catalog #AAA307161) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Parkin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Parkin can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Parkin park2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Parkin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.