Parkin Polyclonal Antibody | anti-PARK2 antibody

Anti-Parkin Antibody

Cat. Num. AAA46524

• Gene Names: PARK2; PDJ; PRKN; AR-JP; LPRS2
• Reactivity: Human, Mouse, Rat
• Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: Parkin, Antibody; Anti-Parkin Antibody; E3 ubiquitin-protein ligase parkin; AR JP; E3 ubiquitin ligase; E3 ubiquitin protein ligase parkin; FRA6E; LPRS 2; LPRS2; PARK 2; PARK2; Parkin 2; Parkinson disease (autosomal recessive juvenile) 2; Parkinson disease (autosomal recessive, juvenile) 2, parkin; Parkinson disease protein 2; Parkinson juvenile disease protein 2; Parkinson protein 2 E3 ubiquitin protein ligase; Parkinson protein 2, E3 ubiquitin protein ligase (parkin); PDJ; PRKN 2; PRKN; PRKN2; PRKN2_HUMAN; Ubiquitin E3 ligase PRKN; parkin RBR E3 ubiquitin protein ligase; anti-PARK2 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: No cross reactivity with other proteins.
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 465

• Applicable Applications for anti-PARK2 antibody: ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Gene Name: parkin RBR E3 ubiquitin protein ligase
• Immunogen: E Coli-derived human Parkin recombinant protein (Position: I23-K416). Human Parkin shares 82% and 84% amino acid (aa) sequence identity with mouse and rat Parkin, respectively.
• Relevant Detection Systems: The lab provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescent kit in WB, supported by in IHC(P) and ICC.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

ICC (Immunocytochemistry)

(Anti- Parkin Picoband antibody, AAA46524, ICCICC: NEURO-2alpha Cell)

IHC (Immunohistochemistry)

(Anti- Parkin Picoband antibody, AAA46524, IHC(P)IHC(P): Human Intestinal Cancer Tissue)

IHC (Immunohistochemisry)

(Anti- Parkin Picoband antibody, AAA46524, IHC(P)IHC(P): Rat Brain Tissue)

IHC (Immunohiostchemistry)

(Anti- Parkin Picoband antibody, AAA46524, IHC(P)IHC(P): Mouse Brain Tissue)

WB (Western Blot)

(Anti- Parkin Picoband antibody, AAA46524, Western blottingAll lanes: Anti Parkin (AAA46524) at 0.5ug/mlLane 1: U87 Whole Cell Lysate at 40ugLane 2: Mouse Brain Tissue Lysate at 50ugPredicted bind size: 66KDObserved bind size: 66KD)

Related Product Information for anti-PARK2 antibody

Background: Parkin is a RING domain-containing E3 ubiquitin ligase involved in proteasome-dependent degradation of proteins. It is mapped to 6q26. This gene is important for mitochondrial quality control by lysosome-dependent degradation of damaged mitochondria through autophagy, or mitophagy. Parkin is expressed in neuronal processes and cell bodies of neurons, but not glial cells, in the midbrain, basal ganglia, cerebral cortex, and cerebellum. Parkin assimilated with actin filaments, suggesting that it is a cytoskeletal-associated protein. Parkin is identified as a transcriptional repressor of p53 independent of its ubiquitin ligase function. It also has been found that parkin was associated physically with mitochondrial DNA (mtDNA) in proliferating as well as in differentiated SH-SY5Y neuroblastoma cells.

References

1. Huynh, D. P., Scoles, D. R., Ho, T. H., Del Bigio, M. R., Pulst, S.-M. Parkin is associated with actin filaments in neuronal and nonneural cells. Ann. Neurol. 48: 737-744, 2000. 2. Kitada T, Asakawa S, Hattori N, Matsumine H, Yamamura Y, Minoshima S et al. (Apr 1998). "Mutations in the parkin gene cause autosomal recessive juvenile parkinsonism". Nature 392 (6676): 605-608. 3. Rothfuss, O., Fischer, H., Hasegawa, T., Maisel, M., Leitner, P., Miesel, F., Sharma, M., Bornemann, A., Berg, D., Gasser, T., Patenge, N. Parkin promotes mitochondrial genome integrity and supports mitochondrial DNA repair. Hum. Molec. Genet. 18: 3832-3850, 2009.

NCBI and Uniprot Product Information

• NCBI GI #: 169790969
• NCBI GeneID: 5071
• NCBI Accession #: NP_004553.2
• NCBI GenBank Nucleotide #: NM_004562.2
• UniProt Accession #: O60260; Q5TFV8; Q5VVX4; Q6Q2I6; Q8NI41; Q8NI43; Q8NI44; Q8WW07; A3FG77; A8K975; D3JZW7; D3K2X0
• NCBI Official Full Name: E3 ubiquitin-protein ligase parkin isoform 1
• NCBI Official Synonym Full Names: parkin RBR E3 ubiquitin protein ligase
• NCBI Official Symbol: PARK2
• NCBI Official Synonym Symbols: PDJ; PRKN; AR-JP; LPRS2
• NCBI Protein Information: E3 ubiquitin-protein ligase parkin
• UniProt Protein Name: E3 ubiquitin-protein ligase parkin
• UniProt Gene Name: PARK2
• UniProt Synonym Gene Names: PRKN; Parkin; Parkinson disease protein 2
• UniProt Entry Name: PRKN2_HUMAN