WB (Western Blot)
(Western blot analysis of P19 nuclear extract (lane 1), P19 nuclear extract treated with retinoic acid (lane 2) and mouse ES nuclear extract (lane 3) probed with NANOG antibody (AAA50475) at a 1/500 dilution.)
Rabbit anti-Mouse NANOG Polyclonal Antibody | anti-Nanog antibody
NANOG antibody
Gene Names
Nanog; ENK; ecat4; 2410002E02Rik
Reactivity
Mouse
Applications
Western Blot, Immunofluorescence, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Antiserum to mouse NANOG was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Synonyms
NANOG, Antibody; NANOG antibody; RABBIT ANTI MOUSE NANOG; anti-Nanog antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antiserum to mouse NANOG was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Form/Format
Purified IgG - liquid
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Phosphate buffered saline
0.035% Sodium Azide (NaN3), 30% Glycerol
Applicable Applications for anti-Nanog antibody
WB (Western Blot), IF (Immunofluorescence), ChIP (Chromatin immunoprecipitation)
Immunogen
A peptide within the N-terminal region of mouse NANOG
Preparation and Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20 degree C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8 degree C for short term use (up to 4 weeks) and store the remaining aliquots at -20 degree C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
WB (Western Blot)
(Western blot analysis of P19 nuclear extract (lane 1), P19 nuclear extract treated with retinoic acid (lane 2) and mouse ES nuclear extract (lane 3) probed with NANOG antibody (AAA50475) at a 1/500 dilution.)
WB (Western Blot)
(Western blot analysis of P19 nuclear extract (lane 1), P19 nuclear extract treated with retinoic acid (lane 2) and mouse ES nuclear extract (lane 3) probed with NANOG antibody (AAA50475) at a 1/500 dilution.)
IF (Immunofluorescence)
(Immunofluorescence staining of mouse embryonic stem cells (mESCs) grown on mouse embryonic fibroblast feeder cells (MEFs) probed with NANOG antibody (AAA50475) at a 1/200 dilution.)
IF (Immunofluorescence)
(Immunofluorescence staining of mouse embryonic stem cells (mESCs) grown on mouse embryonic fibroblast feeder cells (MEFs) probed with NANOG antibody (AAA50475) at a 1/200 dilution.)
Related Product Information for anti-Nanog antibody
Rabbit anti Mouse NANOG antibody recognizes homeobox protein NANOG, otherwise known as homeobox transcription factor NANOG.
NANOG plays a role in the proliferation and self-renewal of embryonic stem (ES) cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages (Barral et al. 2019). Overexpression of NANOG has been shown to promote cells to enter into S phase and proliferation
NANOG plays a role in the proliferation and self-renewal of embryonic stem (ES) cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages (Barral et al. 2019). Overexpression of NANOG has been shown to promote cells to enter into S phase and proliferation
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,240 Da
NCBI Official Full Name
homeobox protein NANOG isoform 2
NCBI Official Synonym Full Names
Nanog homeobox
NCBI Official Symbol
Nanog
NCBI Official Synonym Symbols
ENK; ecat4; 2410002E02Rik
NCBI Protein Information
homeobox protein NANOG; ES cell-associated protein 4; homeobox transcription factor Nanog; early embryo specific expression NK family; early embryo specific expression NK-type homeobox protein
UniProt Protein Name
Homeobox protein NANOG
UniProt Gene Name
Nanog
UniProt Synonym Gene Names
Ecat4; Enk
UniProt Entry Name
NANOG_MOUSE
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Product Notes
The Nanog nanog (Catalog #AAA50475) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NANOG antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NANOG can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), ChIP (Chromatin immunoprecipitation). Researchers should empirically determine the suitability of the Nanog nanog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NANOG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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