FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of NCCIT cells with Nanog antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
Rabbit anti-Human, Mouse Nanog Monoclonal Antibody | anti-NANOG antibody
Nanog Antibody
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
Nanog, Antibody; Nanog Antibody; Embryonic stem cell specific homeobox protein (Nanog) antibody; ENK antibody; FLJ12581 antibody; hNanog antibody; Homeobox protein NANOG antibody; Homeobox transcription factor Nanog antibody; homeobox transcription factor Nanog-delta 48 antibody; NANOG antibody; Nanog homeobox antibody; NANOG_HUMAN antibody; anti-NANOG antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Monoclonal
Clone Number
SC05-70
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-NANOG antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of NCCIT cells with Nanog antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of NCCIT cells with Nanog antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry)
(ICC staining Nanog in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining Nanog in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
WB (Western Blot)
(Western blot analysis of Nanog on F9 cell lysates using anti-Nanog antibody at 1/1, 000 dilution.)
WB (Western Blot)
(Western blot analysis of Nanog on F9 cell lysates using anti-Nanog antibody at 1/1, 000 dilution.)
Related Product Information for anti-NANOG antibody
Nanog (from Tir Na Nog, the mythologic Celtic land of the ever young) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst. High levels of human Nanog expression have been detected by Northern analysis in the undifferentiated NTERA-2 cl.D1 embryonal carcinoma cell line.
Product Categories/Family for anti-NANOG antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
305
NCBI Official Full Name
Homeobox protein NANOG
NCBI Official Synonym Full Names
Nanog homeobox
NCBI Official Symbol
NANOG
NCBI Protein Information
homeobox protein NANOG; homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48
UniProt Protein Name
Homeobox protein NANOG
UniProt Gene Name
NANOG
UniProt Synonym Gene Names
hNanog
UniProt Entry Name
NANOG_HUMAN
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Product Notes
The NANOG nanog (Catalog #AAA312248) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nanog Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nanog can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the NANOG nanog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nanog, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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