IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded mouse testis using PIWIL1 antibody at dilution of 1:100 (40x lens).)
Rabbit MIWI Polyclonal Antibody | anti-PIWIL1 antibody
MIWI Polyclonal Antibody
Gene Names
Piwil1; MIWI
Reactivity
Human, Mouse, Rat
Applications
Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity Purification
Synonyms
MIWI, Antibody; MIWI Polyclonal Antibody; Piwil1; Miwi; PIWIL1; anti-PIWIL1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Concentration
1mg/ml (varies by lot)
Sequence Length
862
Applicable Applications for anti-PIWIL1 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Species
Mouse
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of mouse MIWI
Calculated Molecular Weight
99kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded mouse testis using PIWIL1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded mouse testis using PIWIL1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded rat testis using PIWIL1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded rat testis using PIWIL1 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using PIWIL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using PIWIL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Related Product Information for anti-PIWIL1 antibody
The Drosophila piwi gene was identified as being required for the self-renewal of germline stem cells (1). Piwi homologs are well conserved among various species including Arabidopsis, C. elegans, and Homo sapiens (1). Both Miwi and Mili proteins are mouse homologs of Piwi and contain a C-terminal Piwi domain (2). Miwi and Mili bind to Piwi-interacting RNAs (piRNAs) in male germ cells and are essential for spermatogenesis in mice (3-5).
Product Categories/Family for anti-PIWIL1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
862
NCBI Official Full Name
Piwi-like protein 1
NCBI Official Synonym Full Names
piwi-like RNA-mediated gene silencing 1
NCBI Official Symbol
Piwil1
NCBI Official Synonym Symbols
MIWI
NCBI Protein Information
piwi-like protein 1; piwi like homolog 1; piwi-like homolog 1
UniProt Protein Name
Piwi-like protein 1
UniProt Gene Name
Piwil1
UniProt Synonym Gene Names
Miwi
UniProt Entry Name
PIWL1_MOUSE
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Product Notes
The PIWIL1 piwil1 (Catalog #AAA36919) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MIWI Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MIWI can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the PIWIL1 piwil1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MIWI, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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