IHC (Immunohiostchemistry)
(IHC image of diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
Rabbit anti-Human MAP3K7 Polyclonal Antibody | anti-MAP3K7 antibody
Rabbit anti-Human MAP3K7 Polyclonal Antibody
Gene Names
MAP3K7; TAK1; MEKK7; TGF1a
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Antigen Affinity Purified
Synonyms
MAP3K7, Antibody; Rabbit anti-Human MAP3K7 Polyclonal Antibody; Mitogen-activated protein kinase kinase kinase 7 (EC 2.7.11.25) (Transforming growth factor-beta-activated kinase 1) (TGF-beta-activated kinase 1); MAP3K7; TAK1; MAP3K7 (Ab-187) Antibody; anti-MAP3K7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
Applicable Applications for anti-MAP3K7 antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen Species
Human
Immunogen
Synthesized peptide derived from Human Mitogen-activated protein kinase kinase kinase 7 protein (179-194aa)
Conjugation
Non-conjugated
Preparation and Storage
Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.
IHC (Immunohiostchemistry)
(IHC image of diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
IHC (Immunohiostchemistry)
(IHC image of diluted at 1:100 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.)
WB (Western Blot)
(Western BlotPositive WB detected in: K562 whole cell lysate, Hela whole cell lysate, A549 whole cell lysateAll lanes: MAP3K7 antibody at 1:500SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 68, 65, 57, 54 kDaObserved band size: 68 kDa)
WB (Western Blot)
(Western BlotPositive WB detected in: K562 whole cell lysate, Hela whole cell lysate, A549 whole cell lysateAll lanes: MAP3K7 antibody at 1:500SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 68, 65, 57, 54 kDaObserved band size: 68 kDa)
Related Product Information for anti-MAP3K7 antibody
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK. MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2-induced apoptosis. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B. Promotes TRIM5 capsid-specific restriction activity.
Product Categories/Family for anti-MAP3K7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67,196 Da
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 7 isoform A
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 7
NCBI Official Symbol
MAP3K7
NCBI Official Synonym Symbols
TAK1; MEKK7; TGF1a
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 7; TGF-beta activated kinase 1; TGF-beta-activated kinase 1; transforming growth factor-beta-activated kinase 1
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 7
UniProt Gene Name
MAP3K7
UniProt Synonym Gene Names
TAK1; TGF-beta-activated kinase 1
UniProt Entry Name
M3K7_HUMAN
Similar Products
Product Notes
The MAP3K7 map3k7 (Catalog #AAA244097) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Human MAP3K7 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAP3K7 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the MAP3K7 map3k7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP3K7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.