IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cell using ENO1 antibody. Blue: DAPI for nuclear staining.)
Rabbit ENO1 Polyclonal Antibody | anti-ENO1 antibody
ENO1 Antibody
Gene Names
ENO1; NNE; PPH; MPB1; ENO1L1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Antibodies were purified by affinity purification using immunogen.
Synonyms
ENO1, Antibody; ENO1 Antibody; ENO1; ENO1L1; MBP-1; MPB1; NNE; anti-ENO1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
The antibody detects endogenous level of total ENO1 protein.
Purity/Purification
Antibodies were purified by affinity purification using immunogen.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
341
Applicable Applications for anti-ENO1 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Type
Recombinant Protein
Immunogen Description
Recombinant protein of human ENO1.
Target Name
ENO1
Preparation and Storage
Store at -20 degree C
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cell using ENO1 antibody. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of HeLa cell using ENO1 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using ENO1 antibody.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using ENO1 antibody.)
Related Product Information for anti-ENO1 antibody
Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate. Mammalian enolase exists as three subunits: enolase-1 (a-enolase), enolase-2 (g-enolase) and enolase-3 (b-enolase) that can form both homo- and heterodimers. Expression of the enolase isoforms differs in a tissue specific manner (1). Enolase-1 plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion (2,3). Abnormal expression of enolase-1 is associated with tumor progression in some cases of breast and lung cancer (4-7). Alternatively, an enolase-1 splice variant (MBP-1) binds the c-myc promoter p2 and may function as a tumor suppressor. For this reason enolase-1 is considered as a potential therapeutic target in the treatment of some forms of cancer (8).
Product Categories/Family for anti-ENO1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
36,928 Da
NCBI Official Full Name
c-myc promoter-binding protein-1 isoform MBP-1
NCBI Official Synonym Full Names
enolase 1, (alpha)
NCBI Official Symbol
ENO1
NCBI Official Synonym Symbols
NNE; PPH; MPB1; ENO1L1
NCBI Protein Information
alpha-enolase; c-myc promoter-binding protein-1
UniProt Protein Name
Alpha-enolase
UniProt Gene Name
ENO1
UniProt Synonym Gene Names
ENO1L1; MBPB1; MPB1; NNE
UniProt Entry Name
ENOA_HUMAN
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Product Notes
The ENO1 eno1 (Catalog #AAA305624) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ENO1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ENO1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the ENO1 eno1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ENO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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