IHC (Immunohistochemisry)
(DUT Antibody (C-term) (AAA289098)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DUT Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit DUT Polyclonal Antibody | anti-DUT antibody
DUT Antibody (C-term)
Gene Names
DUT; dUTPase
Reactivity
Human (Predicted Reactivity: Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
DUT, Antibody; DUT Antibody (C-term); Deoxyuridine 5'-triphosphate nucleotidohydrolase; mitochondrial; dUTPase; dUTP pyrophosphatase; DUT; anti-DUT antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This DUT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 170-198 amino acids from the C-terminal region of human DUT.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
170-198
Sequence Length
164
Applicable Applications for anti-DUT antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(DUT Antibody (C-term) (AAA289098)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DUT Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(DUT Antibody (C-term) (AAA289098)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DUT Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysate from A431 cell line, using DUT Antibody (C-term). AAA289098 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysate from A431 cell line, using DUT Antibody (C-term). AAA289098 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
WB (Western Blot)
(Anti-DUT Antibody (C-term)at 1:1000 dilution + SW620 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 26. 5 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-DUT Antibody (C-term)at 1:1000 dilution + SW620 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 26. 5 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-DUT antibody
This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.
References
Takatori, H., et al. Liver Int. 30(3):438-446(2010) Quesada-Soriano, I., et al. Biochimie 92(2):178-186(2010) Chanson, A., et al. Am. J. Clin. Nutr. 89(6):1927-1936(2009) Takacs, E., et al. FEBS Lett. 583(5):865-871(2009) Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26563
NCBI Official Full Name
deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial isoform 1
NCBI Official Synonym Full Names
deoxyuridine triphosphatase
NCBI Official Symbol
DUT
NCBI Official Synonym Symbols
dUTPase
NCBI Protein Information
deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
UniProt Protein Name
Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
UniProt Gene Name
DUT
UniProt Synonym Gene Names
dUTPase
UniProt Entry Name
DUT_HUMAN
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Product Notes
The DUT dut (Catalog #AAA289098) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 170-198. The DUT Antibody (C-term) reacts with Human (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's DUT can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the DUT dut for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DUT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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