IHC (Immunohistochemisry)
(Fig: 3 Immunohistochemical analysis of Cre in a formalin-fixed, paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse using at 1: 2000. In this mouse line, Cre is expressed in the neurons brain. This mouse line also contains a LacZ reporter gene that becomes transcriptionally active upon Cre-mediated recombination. Brain tissue slices were stained with X-gal (blue color) prior to embedding. Cre staining is brown. Nuclear Red counterstain.)
Rabbit Cre Polyclonal Antibody | anti-Cre antibody
Polyclonal Antibody to Cre
Gene Names
TOP1; TOPI
Reactivity
Cre/lox Systems
Applications
Immunoprecipitation, Immunohistochemistry, Western Blot
Purity
Serum
Synonyms
Cre, Antibody; Polyclonal Antibody to Cre; TOP1; DNA topoisomerase I; Antibody to Cre; Anti-Cre Antibody; Cre Antibody; anti-Cre antibody
Host
Rabbit
Reactivity
Cre/lox Systems
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Serum
Form/Format
50 ul sera
Sequence Length
765
Applicable Applications for anti-Cre antibody
IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen Information
A synthethic peptide of the bacteriophage protein Cre (amino acids 57-73 KLNNRKWFPAEPEDVRD) was used as immunogen for this antibody
Antigen
Cre
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
For long-term storage, store at -20 degree C.
Avoid repeated freeze and thaw cycles.
IHC (Immunohistochemisry)
(Fig: 3 Immunohistochemical analysis of Cre in a formalin-fixed, paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse using at 1: 2000. In this mouse line, Cre is expressed in the neurons brain. This mouse line also contains a LacZ reporter gene that becomes transcriptionally active upon Cre-mediated recombination. Brain tissue slices were stained with X-gal (blue color) prior to embedding. Cre staining is brown. Nuclear Red counterstain.)
IHC (Immunohistochemisry)
(Fig: 3 Immunohistochemical analysis of Cre in a formalin-fixed, paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse using at 1: 2000. In this mouse line, Cre is expressed in the neurons brain. This mouse line also contains a LacZ reporter gene that becomes transcriptionally active upon Cre-mediated recombination. Brain tissue slices were stained with X-gal (blue color) prior to embedding. Cre staining is brown. Nuclear Red counterstain.)
IHC (Immunohiostchemistry)
(Fig: 2 Immunohistochemical analysis of Cre in a formalin-fixed, paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse using at 1: 2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. The arrow denotes an example of a stained neuron. Cre staining is also seen in the cytosol of cerebellar Purkinje cells. Hematoxylin-eosin counterstain.)
IHC (Immunohiostchemistry)
(Fig: 2 Immunohistochemical analysis of Cre in a formalin-fixed, paraffin embedded tissue section from the cerebellum of a Cre transgenic mouse using at 1: 2000. In this mouse line, Cre is expressed in the brain, predominantly in the nuclei of most types of neurons. The arrow denotes an example of a stained neuron. Cre staining is also seen in the cytosol of cerebellar Purkinje cells. Hematoxylin-eosin counterstain.)
WB (Western Blot)
(Fig: 1 Western blot analysis of Cre using at 1: 2000. Lane 1, HeLa cells stably transfected with a CRE expression plasmid. Lane 2, HeLa cells transfected with a neo plasmid (negative control).)
WB (Western Blot)
(Fig: 1 Western blot analysis of Cre using at 1: 2000. Lane 1, HeLa cells stably transfected with a CRE expression plasmid. Lane 2, HeLa cells transfected with a neo plasmid (negative control).)
Related Product Information for anti-Cre antibody
This antibody recognizes a 343 amino acid recombinase protein. The Cre (cyclization recombination) enzyme, a member of a large family of recombinases, recognizes loxP which is a sequence motif of 34 bp from the PI bateriophage. If a DNA seqment is flanked by two loxP sites in the same orientation, Cre excises that seqment leaving a single loxP site in the remaining DNA molecule. The excised DNA segment is subsequently degraded. Cre and lox comprise the Cre/lox system which was first developed in the late 1980's to artificially manipulate gene expression (reviewed in Branda and Dymecki, 2004; Ghosh and Duyne, 2002; and Gilbertson, 2003). As an example, mice with the Cre protein expressing in a specific cell type are bred with mice that contain a target gene surrounded by loxP sites. When the mice are bred, the cells carrying Cre will cause those cells to lose the target gene. If the Cre gene is, for example, bound to a promoter that only allows Cre production in neuronal cells, the target gene will be deleted only in those cells. Since 1989 the Cre/lox system has been used extensively and there are numerous animal, plant and bacterial stocks that already contain the Cre gene driven by ubiquitous, tissue-specific or inducible promoters and provide a quick method for breeding experiments. The Cre/lox system has the advantage of working in almost any type of cell. This has led to its application in many experiments, for example in selectively labeling neuronal cells in the brain thereby differentiating them from other types of surrounding cells.
Product Categories/Family for anti-Cre antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
90,726 Da
NCBI Official Full Name
DNA topoisomerase 1
NCBI Official Synonym Full Names
topoisomerase (DNA) I
NCBI Official Symbol
TOP1
NCBI Official Synonym Symbols
TOPI
NCBI Protein Information
DNA topoisomerase 1
UniProt Protein Name
DNA topoisomerase 1
UniProt Gene Name
TOP1
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Product Notes
The Cre top1 (Catalog #AAA78334) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Cre reacts with Cre/lox Systems and may cross-react with other species as described in the data sheet. AAA Biotech's Cre can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the Cre top1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cre, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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