WB (Western Blot)
(Western blot analysis of different cell lines lysates using mouse mAB to annexin A5 dilution 1:2,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] canine A72, [7] equine NBL6, and [8] African green monkey COS1 cells.)
Rabbit Annexin A5 Polyclonal Antibody | anti-A5 antibody
Annexin A5
Gene Names
ANXA5; PP4; ANX5; ENX2; RPRGL3; HEL-S-7
Reactivity
Human, Mouse, Rat, Dog, Equine
Applications
Immunofluorescence, Western Blot, Immunocytochemistry
Synonyms
Annexin A5, Antibody; Annexin A5; anti-A5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog, Equine
Clonality
Polyclonal
Isotype
Rabbit IgG
Form/Format
Affinity Purified antibody
Liquid. Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Liquid. Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-A5 antibody
IF (Immunofluorescence), WB (Western Blot), ICC (Immunocytochemistry)
Immunogen
Full length human annexin A5 expressed in and purified from E Coli
Preparation and Storage
Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for six months without detectable loss of activity. The antibody is stableat 2-8 degree C for 1 year. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of different cell lines lysates using mouse mAB to annexin A5 dilution 1:2,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] canine A72, [7] equine NBL6, and [8] African green monkey COS1 cells.)
WB (Western Blot)
(Western blot analysis of different cell lines lysates using mouse mAB to annexin A5 dilution 1:2,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] canine A72, [7] equine NBL6, and [8] African green monkey COS1 cells.)
IF (Immunofluorescence)
(Immunofluorescent analysis of HeLa cells stained with mouse mAb to annexin A5, MO22200, dilution 1:1,000 in green, and costained with chicken pAb to vimentin dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA.)
IF (Immunofluorescence)
(Immunofluorescent analysis of HeLa cells stained with mouse mAb to annexin A5, MO22200, dilution 1:1,000 in green, and costained with chicken pAb to vimentin dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA.)
Related Product Information for anti-A5 antibody
The annexins are a large family of related proteins which share the property of binding to phophospholipid containing membranes in a Calcium dependent manner. Different members of the family were discovered by different laboratories and as a result the various members have many alternate names, such as lipocortin, calpactin, calelectrin and very many others. The widely used current nomenclature is now based on a letter to indicate membership in a particular one of several annexin sub-families and a number for individual gene products, hence the name annexin A5. The annexin family is defined by a compact disc structure formed from 16 closely packed alpha-helices which co-ordinate multiple calcium ions with phospholipid containing membranes. This domain is defined by 4 imperfect repeats of a ~77 amino acid sequence, each repeat forming 4 alpha-helices. Annexin A5 is expressed widely in tissues and has been used as a marker of apoptosis, as apoptotic cells may express binding sites for this protein on their cell surface. The protein binds to phosphatidylserine, a membrane lipid normally not found on the external surface of cells which becomes expressed on the cell surface during apoptosis. As a result fluorescent annexin A5 or annexin A5 antibody can be use to isolate apoptotic cells by fluorescence activated cell sorting.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,937 Da
NCBI Official Full Name
annexin A5
NCBI Official Synonym Full Names
annexin A5
NCBI Official Symbol
ANXA5
NCBI Official Synonym Symbols
PP4; ANX5; ENX2; RPRGL3; HEL-S-7
NCBI Protein Information
annexin A5; CBP-I; PAP-I; VAC-alpha; annexin V; annexin-5; anchorin CII; endonexin II; lipocortin V; calphobindin I; thromboplastin inhibitor; vascular anticoagulant-alpha; epididymis secretory protein Li 7; placental anticoagulant protein 4; placental an
UniProt Protein Name
Annexin A5
UniProt Gene Name
ANXA5
UniProt Synonym Gene Names
ANX5; ENX2; PP4; CBP-I; PP4; PAP-I; VAC-alpha
UniProt Entry Name
ANXA5_HUMAN
Similar Products
Product Notes
The A5 anxa5 (Catalog #AAA76693) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Annexin A5 reacts with Human, Mouse, Rat, Dog, Equine and may cross-react with other species as described in the data sheet. AAA Biotech's Annexin A5 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the A5 anxa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin A5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.