WB (Western Blot)
(Western blot of A431 cell lysate only (lane 1), AM0171 antibody only (lane 2), and AM0171 antibody immunoprecipitate from A431(lane 3). The blot was probed with mouse monoclonal anti-Annexin A5 antibody (AM0171) at 1:500 (lanes 1-3). The asterisk shows the antibody heavy chain in immunopreciptates at 50kDa, while Annexin A5 band is observed at 35kDa.)
Mouse anti-Human Annexin A5 Monoclonal Antibody | anti-A5 antibody
Annexin A5 Antibody
Gene Names
ANXA5; PP4; ANX5; ENX2; RPRGL3; HEL-S-7
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Synonyms
Annexin A5, Antibody; Annexin A5 Antibody; Annexin A5; anti-A5 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
M017
Specificity
Clone M017 detects a 35kDa* protein corresponding to the molecular mass of Annexin A5 on SDS-PAGE immunoblots of human A431, MDA-MB-231, MeWo, and PC3 cells, as well as human skin, breast, lung, and brain. The antibody works for ELISA, western blot, and immunoprecipitation. In addition, mass spectrometry analysis of immunoprecipitates using AM0171 in human MDA-MB-231 cell lysates confirms this antibody only detects Annexin A5 protein.
Form/Format
Mouse monoclonal, protein G purified antibody is supplied in 100ul phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide.
Applicable Applications for anti-A5 antibody
IP-MS (Immunoprecipitation-MassSpectrometry), ELISA, WB (Western Blot)
Immunogen
Clone M017 was generated from a proprietary antigen related to human Annexin A5 in the MDA-MB-231 breast cancer cell line.
Preparation and Storage
Store at -20 degree C. Stable for 1 year.
WB (Western Blot)
(Western blot of A431 cell lysate only (lane 1), AM0171 antibody only (lane 2), and AM0171 antibody immunoprecipitate from A431(lane 3). The blot was probed with mouse monoclonal anti-Annexin A5 antibody (AM0171) at 1:500 (lanes 1-3). The asterisk shows the antibody heavy chain in immunopreciptates at 50kDa, while Annexin A5 band is observed at 35kDa.)
WB (Western Blot)
(Western blot of A431 cell lysate only (lane 1), AM0171 antibody only (lane 2), and AM0171 antibody immunoprecipitate from A431(lane 3). The blot was probed with mouse monoclonal anti-Annexin A5 antibody (AM0171) at 1:500 (lanes 1-3). The asterisk shows the antibody heavy chain in immunopreciptates at 50kDa, while Annexin A5 band is observed at 35kDa.)
WB (Western Blot)
(Western blot of human PC3 cells (lane 1), human breast tissue (lane 2), lung tissue (lane 3), skin tissue (lane 4), and brain tissue (lane 5). The blot was probed with mouse monoclonal anti-Annexin A5 antibody (AM0171) at 1:500 (lanes 1-5).)
WB (Western Blot)
(Western blot of human PC3 cells (lane 1), human breast tissue (lane 2), lung tissue (lane 3), skin tissue (lane 4), and brain tissue (lane 5). The blot was probed with mouse monoclonal anti-Annexin A5 antibody (AM0171) at 1:500 (lanes 1-5).)
Related Product Information for anti-A5 antibody
The Annexin family is composed of at least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. Annexins have roles in membrane fusion, endocytosis, secretion, and repair. Annexin A1 binds to cellular membranes in a calcium-dependent manner, promotes membrane fusion and endocytosis, and has been implicated as an anti-inflammatory mediator. Annexin A2 is a cytoskeletal calcium-dependent phospholipid binding protein, which has been shown to be a mediator of corticosteroid activity, a substrate for serine/threonine kinases and growth regulated tyrosine kinases, and may play a role in secretion. Annexin A5 is a PKC inhibitor, directly interacts with VEGFR2 receptor, and binds phosphatidylserine to inhibit blood coagulation. Annexin A6 reverses transformation of A431 cells after overexpression, and this effect may involve annexin A6 targeting of p120 RasGAP to the plasma membrane to inactivate Ras.
References
Boye TL & Nylandsted J (2016) Biol Chem. 397(10):961.
Hoque M et al. (2014) Cell Signal. 26(6):1213.
Grewal T & Enrich C (2009) Cell Signal. 21(6):847.
Hoque M et al. (2014) Cell Signal. 26(6):1213.
Grewal T & Enrich C (2009) Cell Signal. 21(6):847.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,937 Da
NCBI Official Full Name
annexin A5
NCBI Official Synonym Full Names
annexin A5
NCBI Official Symbol
ANXA5
NCBI Official Synonym Symbols
PP4; ANX5; ENX2; RPRGL3; HEL-S-7
NCBI Protein Information
annexin A5; CBP-I; PAP-I; VAC-alpha; annexin V; annexin-5; anchorin CII; endonexin II; lipocortin V; calphobindin I; thromboplastin inhibitor; vascular anticoagulant-alpha; epididymis secretory protein Li 7; placental anticoagulant protein 4; placental an
UniProt Protein Name
Annexin A5
UniProt Gene Name
ANXA5
UniProt Synonym Gene Names
ANX5; ENX2; PP4; CBP-I; PP4; PAP-I; VAC-alpha
UniProt Entry Name
ANXA5_HUMAN
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Product Notes
The A5 anxa5 (Catalog #AAA71556) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Annexin A5 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Annexin A5 can be used in a range of immunoassay formats including, but not limited to, IP-MS (Immunoprecipitation-MassSpectrometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the A5 anxa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin A5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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