Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts of various sample, using Catenin-beta antibody.)

IHC (Immunohistochemistry) (AAA31090 showing positive staining in colon cancer tissue.)

IF (Immunofluorescence) (AAA31090 staining SW626 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Catenin-beta expression in 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA31090 staining MCF-7 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts of various sample, using Catenin-beta antibody.)

Compare (5 max)

Catenin-beta, Polyclonal Antibody (Cat# AAA31090)

Full Name

Catenin-beta Antibody

Gene Names

CTNNB1; EVR7; CTNNB; MRD19; armadillo

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Figure 13. IF analysis of STIM1 using anti-STIM1 antibody (AAA19731).STIM1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 12. IF analysis of STIM1 using anti-STIM1 antibody (AAA19731).STIM1 was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 11. IF analysis of STIM1 using anti-STIM1 antibody (AAA19731).STIM1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of K562 cells using anti-STIM1 antibody (AAA19731).Overlay histogram showing K562 cells stained with AAA19731 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-STIM1 Antibody (AAA19731, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of STIM1 using anti-STIM1 antibody (AAA19731).STIM1 was detected in a paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-STIM1 Antibody (AAA19731) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: rat PC-12 whole cell lysates,Lane 5: mouse liver tissue lysates,Lane 6: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STIM1 antigen affinity purified polyclonal antibody (#AAA19731) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STIM1 at approximately 85 kDa. The expected band size for STIM1 is at 77 kDa.)

Compare (5 max)

STIM1, Polyclonal Antibody (Cat# AAA19731)

Full Name

Anti-STIM1 Antibody Picoband

Gene Names

STIM1; GOK; TAM; TAM1; IMD10; STRMK; D11S4896E

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

WB (Western Blot) (Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)

IP (Immunoprecipitation) (Immunoprecipitation: 10ug of was used to immunoprecipitate from 500ug of Jurkat whole cell lysate. The antibody was collected on Protein A beads and eluted with sample buffer. 5uL of Jurkat whole cell lysate was then resolved via SDS-PAGE, transferred to PVDF and probed with 0.5ug . Proteins were visualized using anti-rabbit-HRP conjugate and an ECL system.)

ICC (Immunocytochemistry) (Immunocytochemistry: PI3-Kinase, p110b staining of human skeletal muscle. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern of cross banding striated muscle fibers.)

ICC (Immunocytochemistry) (Immunocytochemistry: PI3-Kinase, p110b staining in colorectal carcinoma. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a plasma membrane staining pattern.)

ICC (Immunocytochemistry) (Confocal Immunocytochemistry Analysis: HeLa cells were fixed, permeablized, and stained with (Cy3, red), DAPI (blue, nuclei), and Phalloidin-AlexaFluor488 (actin, green). Figure on the left has the DAPI filter off.)

ICC (Immunocytochemistry) (Immunocytochemistry: PI3-Kinase, p110b staining of human kidney 2 mm array spot. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is see as a staining to include the thin and distal microtubules.)

Compare (5 max)

Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26896)

Full Name

Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol

Gene Names

PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha

Reactivity

Human

Applications

Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry

Purity

Purified by Immunoaffinity chromatography.

Pricing

$1,230/0.1 mL $2,380/2x0.1 mL $3,460/3x0.1 mL $4,480/4x0.1 mL $5,260/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,DRP1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,DRP1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,DRP1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-spinal-cord tissue. 1,DRP1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1,DRP1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,DRP1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,DRP1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,DRP1 Polyclonal Antibody was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.)

WB (Western Blot) (Western Blot analysis of various cells using DRP1 Polyclonal Antibody diluted at 1:500)

WB (Western Blot) (Western Blot analysis of HELA cells using DRP1 Polyclonal Antibody diluted at 1:500)

Application Data (Experimental and therapeutic medicine 17.3 (2019): 2349-2358.)

Compare (5 max)

DRP1, Polyclonal Antibody (Cat# AAA30750)

Full Name

DRP1 Rabbit Polyclonal Antibody

Gene Names

DNM1L; DLP1; DRP1; DVLP; EMPF; DYMPLE; HDYNIV

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunocytochemistry, Western Blot, Immunohistochemistry

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$335/0.05 mL $415/0.1 mL $785/2x0.1 mL $1,130/3x0.1 mL $1,455/4x0.1 mL $1,550/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (ICC staining MGMT in MCF-7 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MGMT in SK-BR-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MGMT in A172 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-MGMT antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGMT antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of MGMT on different cell lysates using anti- MGMT antibody at 1/1000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7 Lane 3: Daudi Lane 4: Raji Lane 5: SK-BR-3 Lane 6: SKOV3 Lane 7: Human liver Lane 8: Human kidney Lane 9: Human thymus Lane 10: Human placentae)

Compare (5 max)

MGMT, Polyclonal Antibody (Cat# AAA29958)

Full Name

MGMT Antibody

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

Peptide affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of PC12 cells using EGFR antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using EGFR antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using EGFR antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using EGFR antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate using EGFR antibody.)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and EGFR knockout (KO) HeLa cells, using EGFR antibody.)

WB (Western Blot) (Western blot analysis of extracts of OVCAR3 cells, using EGFR antibody.)

Compare (5 max)

EGFR, Polyclonal Antibody (Cat# AAA29798)

Full Name

EGFR Polyclonal Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

PDGF-B, Polyclonal Antibody (Cat# AAA29094)

Full Name

PDGF-B Polyclonal Antibody

Gene Names

PDGFB; SIS; SSV; PDGF2; c-sis; PDGF-2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistchemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

Compare (5 max)

MIP-3alpha, Polyclonal Antibody (Cat# AAA29367)

Full Name

MIP-3alpha Polyclonal Antibody

Gene Names

CCL20; CKb4; LARC; ST38; MIP3A; Exodus; MIP-3a; SCYA20; MIP-3-alpha

Reactivity

Human, Mouse

Applications

Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Rat spleen tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of p70 S6 Kinase (Phospho-Ser427) using HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

WB (Western Blot) (Western blot analysis of extracts from HepG(serum starvation treatment), using Phospho-p70 S6 Kinase (Ser427) Antibody. The lane on the left was treated with blocking peptide.)

Compare (5 max)

p70 S6 Kinase, Polyclonal Antibody (Cat# AAA31408)

Full Name

Phospho-p70 S6 Kinase (Ser427) Antibody

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Mouse stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Compare (5 max)

MAP3K3, Polyclonal Antibody (Cat# AAA31283)

Full Name

Phospho-MAP3K3 (Ser166) Antibody

Gene Names

MAP3K3; MEKK3; MAPKKK3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

PTN, Polyclonal Antibody (Cat# AAA29284)

Full Name

PTN Polyclonal Antibody

Gene Names

PTN; HARP; HBNF; HBGF8; NEGF1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells treated with EGF, HWEC cells treated with VEGF and JK cells using eNOS (Phospho-Ser1177) Antibody #AAA29721.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using eNOS(Phospho-Ser1177) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eNOS(Phospho-Ser1177) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

Compare (5 max)

eNOS, Polyclonal Antibody (Cat# AAA29721)

Full Name

eNOS (Phospho-Ser1177) Antibody

Gene Names

NOS3; eNOS; ECNOS

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

$305/0.05 mL $365/0.1 mL $680/2x0.1 mL $980/3x0.1 mL $1,260/4x0.1 mL $1,355/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human liver cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Acetyl-p53 (K373) in lysates of MDA-MB-435 cells(TSA 1M, 18 hr), using Acetyl-p53 (K373) Antibody(AF4363).)

WB (Western Blot) (Western blot analysis of extracts from MDA-MB-435 cells(TSA 1M, 18 hr), using Acetyl-p53 (Lys373) Antibody.Lane1 was treated with Ac-blocking peptide.Lane2 was treated with Non-Ac-blocking peptide.)

Compare (5 max)

p53, Polyclonal Antibody (Cat# AAA31354)

Full Name

Acetyl-p53 (Lys373) Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (91%), Sheep (80%), Rabbit (91%), Dog (91%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

< >

<< Previous Next >>

Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of AXL (Phospho-Tyr702) using 293 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Compare (5 max)

AXL, Polyclonal Antibody (Cat# AAA31469)

Full Name

Phospho-AXL (Tyr702) Antibody

Gene Names

AXL; UFO; JTK11

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

Application Data

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

Compare (5 max)

AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26878)

Full Name

AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (MaxLight 490)

Gene Names

ACACA; ACC; ACAC; ACC1; ACCA; ACACAD

Reactivity

Mouse, Human

Applications

WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,175/0.1 mL $2,260/2x0.1 mL $3,290/3x0.1 mL $4,265/4x0.1 mL $5,000/5x0.1 mL

< >

<< Previous Next >>

Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody(Red), diluted at 1/600, was used as secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of ATP1A1 (Phospho-Tyr260) using HepG2 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Compare (5 max)

ATP1A1, Polyclonal Antibody (Cat# AAA31454)

Full Name

Phospho-ATP1A1 (Tyr260) Antibody

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (90%), Dog (100%), Xenopus (90%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

< >

<< Previous Next >>

IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of HeLa cells usingug CDK4 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using CDK4 Polyclonal Antibody at a dilution of 1:1000.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using CDK4 Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and CDK4 knockout (KO) HeLa cells, using CDK4 Polyclonal Antibody at 1:1000 dilution.)

Compare (5 max)

CDK4, Polyclonal Antibody (Cat# AAA22305)

Full Name

(KO Validated) CDK4 Polyclonal Antibody

Gene Names

CDK4; CMM3; PSK-J3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purity

Affinity purification

Pricing

$340/0.06 mL $475/0.12 mL $710/0.2 mL $1,365/2x0.2 mL $1,975/3x0.2 mL $2,550/4x0.2 mL $3,100/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using CCDC59 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using CCDC59 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human uterine cancer using CCDC59 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using CCDC59 antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using CCDC59 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung using CCDC59 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using CCDC59 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using CCDC59 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using CCDC59 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CCDC59 antibody.)

Compare (5 max)

CCDC59, Polyclonal Antibody (Cat# AAA29808)

Full Name

CCDC59 Polyclonal Antibody

Gene Names

CCDC59; BR22; TAP26; HSPC128

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using DAPK3 antibody at dilution of 1:100 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human placenta using DAPK3 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using DAPK3 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using DAPK3 antibody at dilution of 1:100 .)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using DAPK3 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using DAPK3 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DAPK3 antibody at 1:1000 dilution.)

Compare (5 max)

DAPK3, Polyclonal Antibody (Cat# AAA30573)

Full Name

DAPK3 Polyclonal Antibody

Gene Names

DAPK3; ZIP; ZIPK

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (DAB staining on IHC-P;Samples: Mouse Kidney Tissue;Primary Ab: 10ug/ml Rabbit Anti-Mouse IFNa AntibodySecond Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Sample: Recombinant IFNa, Mouse.)

Compare (5 max)

Interferon Alpha, Polyclonal Antibody (Cat# AAA21018)

Full Name

Polyclonal Antibody to Interferon Alpha (IFNa)

Gene Names

Ifna1; Ifa1

Reactivity

Mouse

Applications

Westen Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$220/0.1 mL $245/0.2 mL $375/0.5 mL $430/1 mL $1,100/5 mL $2,115/2x5 mL $3,075/3x5 mL $3,980/4x5 mL $4,840/5x5 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of SH-SY5Y cells using anti-Lumican/LUM antibody (AAA19744).Overlay histogram showing SH-SY5Y cells stained with AAA19744 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Lumican/LUM Antibody (AAA19744, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Lumican/LUM using anti-Lumican/LUM antibody (AAA19744).Lumican/LUM was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Lumican/LUM Antibody (AAA19744) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Lumican/LUM Antibody (AAA19744) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Lumican/LUM Antibody (AAA19744) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Lumican/LUM Antibody (AAA19744) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human placenta tissue lysates,Lane 3: human Caco-2 whole cell lysates,Lane 4: rat liver tissue lysates,Lane 5: mouse liver tissue lysates,Lane 6: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lumican/LUM antigen affinity purified polyclonal antibody (#AAA19744) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Lumican/LUM at approximately 70 kDa. The expected band size for Lumican/LUM is at 38 kDa.)

Compare (5 max)

Lumican/LUM, Polyclonal Antibody (Cat# AAA19744)

Full Name

Anti-Lumican/LUM Antibody Picoband

Gene Names

LUM; LDC; SLRR2D

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of A-549 cells using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using Lamin A/C Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of various lysates using Lamin A/C Polyclonal Antibody at 1:1000 dilution.)

Compare (5 max)

Lamin A/C, Polyclonal Antibody (Cat# AAA22292)

Full Name

Lamin A/C Polyclonal Antibody

Gene Names

LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$445/0.12 mL $675/0.2 mL $1,290/2x0.2 mL $1,875/3x0.2 mL $2,420/4x0.2 mL $2,905/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using MAP1LC3B antibody at dilution of 1:100. NIH/3T3 cells were treated by Chloroquine (50 uM) for 20 hours(left). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using MAP1LC3B antibody at dilution of 1:100. Hela cells were treated by Chloroquine (50 uM) for 20 hours(left). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using MAP1LC3B antibody at dilution of 1:100. C6 cells were treated by Chloroquine (50 uM) for 20 hours(left). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using MAP1LC3B antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using MAP1LC3B antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using MAP1LC3B antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of mouse brain, using MAP1LC3B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 2min.)

Compare (5 max)

MAP1LC3B, Polyclonal Antibody (Cat# AAA28283)

Full Name

MAP1LC3B Polyclonal Antibody

Gene Names

MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U251 cells using anti-Acid Phosphatase/ACP5 antibody (AAA19481).Overlay histogram showing U251 cells stained with AAA19481 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Acid Phosphatase/ACP5 Antibody (AAA19481, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of Acid Phosphatase/ACP5 using anti-Acid Phosphatase/ACP5 antibody (AAA19481).Acid Phosphatase/ACP5 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-Acid Phosphatase/ACP5 Antibody (AAA19481) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Acid Phosphatase/ACP5 using anti-Acid Phosphatase/ACP5 antibody (AAA19481).Acid Phosphatase/ACP5 was detected in a paraffin-embedded section of human renal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Acid Phosphatase/ACP5 Antibody (AAA19481) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Acid Phosphatase/ACP5 using anti-Acid Phosphatase/ACP5 antibody (AAA19481).Acid Phosphatase/ACP5 was detected in a paraffin-embedded section of human lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Acid Phosphatase/ACP5 Antibody (AAA19481) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Acid Phosphatase/ACP5 using anti-Acid Phosphatase/ACP5 antibody (AAA19481).Acid Phosphatase/ACP5 was detected in a paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Acid Phosphatase/ACP5 Antibody (AAA19481) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Acid Phosphatase/ACP5 using anti-Acid Phosphatase/ACP5 antibody (AAA19481).Acid Phosphatase/ACP5 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Acid Phosphatase/ACP5 Antibody (AAA19481) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Acid Phosphatase/ACP5 using anti-Acid Phosphatase/ACP5 antibody (AAA19481).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human A431 whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human Hela whole cell lysates,Lane 5: human K562 whole cell lysates,Lane 6: human Raji whole cell lysates,Lane 7: rat lung tissue lysates,Lane 8: mouse lung tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Acid Phosphatase/ACP5 antigen affinity purified polyclonal antibody (#AAA19481) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Acid Phosphatase/ACP5 at approximately 39 kDa. The expected band size for Acid Phosphatase/ACP5 is at 37 kDa.)

Compare (5 max)

Acid phosphatase/ACP5, Polyclonal Antibody (Cat# AAA19481)

Full Name

Anti-Acid phosphatase/ACP5 Antibody Picoband

Gene Names

ACP5; TRAP; SPENCDI

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

PTEN, Polyclonal Antibody (Cat# AAA28948)

Full Name

PTEN (phospho Ser380/T382/T383) Polyclonal Antibody

Gene Names

PTEN; BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from VERO cells(H2O2 treatment), using Phospho-SAMHD1 (Thr592) Antibody. The lane on the left was treated with blocking peptide.)

Compare (5 max)

SAMHD1, Polyclonal Antibody (Cat# AAA31278)

Full Name

Phospho-SAMHD1 (Thr592) Antibody

Gene Names

SAMHD1; DCIP; CHBL2; HDDC1; MOP-5; SBBI88

Reactivity

Human, Mouse, Rat, Monkey

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Daudi cells using anti-Ubc13/UBE2N antibody (AAA19458).Overlay histogram showing Daudi cells stained with AAA19458 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Ubc13/UBE2N Antibody (AAA19458, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of Ubc13/UBE2N using anti-Ubc13/UBE2N antibody (AAA19458).Ubc13/UBE2N was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-Ubc13/UBE2N Antibody (AAA19458) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Ubc13/UBE2N using anti-Ubc13/UBE2N antibody (AAA19458).Ubc13/UBE2N was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Ubc13/UBE2N Antibody (AAA19458) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Ubc13/UBE2N using anti-Ubc13/UBE2N antibody (AAA19458).Ubc13/UBE2N was detected in a paraffin-embedded section of human lung squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Ubc13/UBE2N Antibody (AAA19458) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Ubc13/UBE2N using anti-Ubc13/UBE2N antibody (AAA19458).Ubc13/UBE2N was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Ubc13/UBE2N Antibody (AAA19458) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Ubc13/UBE2N using anti-Ubc13/UBE2N antibody (AAA19458).Ubc13/UBE2N was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Ubc13/UBE2N Antibody (AAA19458) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Ubc13/UBE2N using anti-Ubc13/UBE2N antibody (AAA19458).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Daudi whole cell lysates,Lane 2: human MOLT-4 whole cell lysates,Lane 3: human RT4 whole cell lysates,Lane 4: human HEL whole cell lysates,Lane 5: rat heart tissue lysates,Lane 6: rat thymus tissue lysates,Lane 7: rat C6 whole cell lysates,Lane 8: mouse thymus tissue lysates,Lane 9: mouse small intestine tissue lysates,Lane 10: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ubc13/UBE2N antigen affinity purified polyclonal antibody (#AAA19458) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Ubc13/UBE2N at approximately 17 kDa. The expected band size for Ubc13/UBE2N is at 17 kDa.)

Compare (5 max)

Ubc13/UBE2N, Polyclonal Antibody (Cat# AAA19458)

Full Name

Anti-Ubc13/UBE2N Antibody Picoband

Gene Names

UBE2N; UBC13; UbcH13; HEL-S-71; UbcH-ben; UBCHBEN; UBC13

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

Application Data

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

Compare (5 max)

AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26872)

Full Name

AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (AP)

Gene Names

ACACA; ACC; ACAC; ACC1; ACCA; ACACAD

Reactivity

Mouse, Human

Applications

WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,175/0.1 mL $2,260/2x0.1 mL $3,290/3x0.1 mL $4,265/4x0.1 mL $5,000/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U251 cells using anti-CGKI/PRKG1 antibody (AAA19246).Overlay histogram showing U251 cells stained with AAA19246 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CGKI/PRKG1 Antibody (AAA19246, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of RH35 cells using anti-CGKI/PRKG1 antibody (AAA19246).Overlay histogram showing RH35 cells stained with AAA19246 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CGKI/PRKG1 Antibody (AAA19246, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of CGKI/PRKG1 using anti- CGKI/PRKG1 antibody (AAA19246).CGKI/PRKG1 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-CGKI/PRKG1 Antibody (AAA19246) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of CGKI/PRKG1 using anti-CGKI/PRKG1 antibody (AAA19246).CGKI/PRKG1 was detected in paraffin-embedded section of hman breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CGKI/PRKG1 Antibody (AAA19246) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of CGKI/PRKG1 using anti-CGKI/PRKG1 antibody (AAA19246).CGKI/PRKG1 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CGKI/PRKG1 Antibody (AAA19246) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of CGKI/PRKG1 using anti-CGKI/PRKG1 antibody (AAA19246).CGKI/PRKG1 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CGKI/PRKG1 Antibody (AAA19246) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of CGKI/PRKG1 using anti-CGKI/PRKG1 antibody (AAA19246).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat lung tissue lysatesLane 2: rat heart tissue lysatesLane 3: mouse lung tissue lysatesLane 4: human HEK293 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CGKI/PRKG1 antigen affinity purified polyclonal antibody (Catalog # AAA19246) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for CGKI/PRKG1 at approximately 78KD. The expected band size for CGKI/PRKG1 is at 78KD.)

Compare (5 max)

cGKI/PRKG1, Polyclonal Antibody (Cat# AAA19246)

Full Name

Anti-cGKI/PRKG1 Antibody

Gene Names

PRKG1; 1; PKG; cGK; AAT8; cGK1; cGKI; cGK 1; PRKG1B; PRKGR1B; cGKI-BETA; cGKI-alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts of various cell lines.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PSMA5 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of U20S cell using PSMA5 antibody. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of U20S cells.)

IHC (Immunohistochemistry) (Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (Human Uterus: Formalin-Fixed, Paraffin-Embedded (FFPE))

Compare (5 max)

PSMA5, Polyclonal Antibody (Cat# AAA21278)

Full Name

Anti-PSMA5 Antibody

Gene Names

PSMA5; PSC5; ZETA

Reactivity

Human
Predicted: Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

$690/0.05 mL $1,325/2x0.05 mL $1,915/3x0.05 mL $2,475/4x0.05 mL $2,800/5x0.05 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

JIP-3, Polyclonal Antibody (Cat# AAA29055)

Full Name

JIP-3 Polyclonal Antibody

Gene Names

MAPK8IP3; syd; JIP3; SYD2; JSAP1

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using RUNX1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using RUNX1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using RUNX1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat heart using RUNX1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using RUNX1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using RUNX1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using RUNX1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using RUNX1 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of SH-SY5Y cells, using RUNX1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.)

Compare (5 max)

RUNX1, Polyclonal Antibody (Cat# AAA10677)

Full Name

RUNX1 Polyclonal Antibody

Gene Names

RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat ovarian tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis Smad2 (Phospho-Ser255) using heatshock treated HT29 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Compare (5 max)

Smad2, Polyclonal Antibody (Cat# AAA31390)

Full Name

Phospho-Smad2 (Ser255) Antibody

Gene Names

SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Zebrafish (89%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (89%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using SFPQ antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using SFPQ antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using SFPQ antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse lung using SFPQ antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using SFPQ antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using SFPQ antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using SFPQ antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using SFPQ antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SFPQ antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)

Compare (5 max)

SFPQ, Polyclonal Antibody (Cat# AAA10716)

Full Name

SFPQ Polyclonal Antibody

Gene Names

SFPQ; PSF; POMP100; PPP1R140

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

Application Data (ELISA plate was coated with purified human IgG, IgM, and IgA. Immunoglobulins were detected with serially diluted Goat Anti-Human IgG-HRP.)

Compare (5 max)

Goat Anti-Human IgG (gamma chain specific), Polyclonal Secondary Antibody (Cat# AAA14904)

Full Name

Goat Anti-Human IgG-HRP

Applications

ELISA

Purity

Affinity chromatography on human IgG covalently linked to agarose

Pricing

$245/1 mL $455/2x1 mL $655/3x1 mL $830/5x1 mL $845/4x1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

CD58, Polyclonal Antibody (Cat# AAA29194)

Full Name

CD58 Polyclonal Antibody

Gene Names

CD58; ag3; LFA3; LFA-3

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IHC (Immunohistchemistry) (Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

WB (Western Blot) (Dilution: WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

Compare (5 max)

Glut4, Polyclonal Antibody (Cat# AAA29286)

Full Name

Glut4 Polyclonal Antibody

Gene Names

SLC2A4; GLUT4

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human lung cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis IRF-7 (Phospho-Ser477) using Serum treated LOVO whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Compare (5 max)

IRF7, Polyclonal Antibody (Cat# AAA31458)

Full Name

Phospho-IRF7 (Ser477) Antibody

Gene Names

IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using FLNB antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human esophagus using FLNB antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate using FLNB antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver injury using FLNB antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using FLNB antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using FLNB antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using FLNB antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

Compare (5 max)

FLNB, Polyclonal Antibody (Cat# AAA10740)

Full Name

FLNB Polyclonal Antibody

Gene Names

FLNB; AOI; FH1; SCT; TAP; LRS1; TABP; FLN-B; FLN1L; ABP-278; ABP-280

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Anti-GLP1R/GLP-1R/GLP-1 Receptor antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-GLP1R/GLP-1R/GLP-1 Receptor antibody IHC of human Adrenal, Pheochromocytoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-GLP-1 Receptor / GLP1R antibody IHC of human pancreas, islet of Langerhans. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3-4 ug/ml.)

IHC (Immunohistochemistry) (Anti-GLP1R/GLP-1R/GLP-1 Receptor antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-GLP1R/GLP-1R/GLP-1 Receptor antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-GLP1R/GLP-1R/GLP-1 Receptor antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Compare (5 max)

GLP1R/GLP-1R/GLP-1 Receptor, Polyclonal Antibody (Cat# AAA12337)

Full Name

Rabbit Polyclonal to Human GLP1R/GLP-1R/GLP-1 Receptor

Reactivity

Gibbon, Gorilla, Human
Predicted Reactivity: Monkey, Mouse, Rat, Bovine, Hamster, Pig, Rabbit, Sheep (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$690/0.05 mg $1,325/2x0.05 mg $1,915/3x0.05 mg $2,475/4x0.05 mg $2,800/5x0.05 mg

< >

<< Previous Next >>

WB (Western Blot) (25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Canonical 70 kDa isoform is identified, and a second isoform of 65 kDa is also present in some samples.)

WB (Western Blot) (WB Suggested Anti-SLC27A2 antibody Titration: 1 ug/mLSample Type: Human PANC1)

WB (Western Blot) (WB Suggested Anti-SLC27A2 antibody Titration: 1 ug/mLSample Type: Human MCF7)

WB (Western Blot) (WB Suggested Anti-SLC27A2 Antibody Titration: 1 ug/mlPositive Control: Jurkat lysate)

WB (Western Blot) (WB Suggested Anti-SLC27A2 Antibody Titration: 1 ug/mlPositive Control: Hela cell lysate)

WB (Western Blot) (Host: RabbitTarget Name: SLC27A2Sample Type: HelaLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5.0 ug/mlLysate Quantity: 25ug/lane/laneGel Concentration: 12%SLC27A2 is strongly supported by BioGPS gene expression data to be expressed in Human HeLa cells)

IHC (Immunohistochemistry) (Liver, Human: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (Liver, Human: Formalin-Fixed, Paraffin-Embedded (FFPE))

Compare (5 max)

SLC27A2, Polyclonal Antibody (Cat# AAA23511)

Full Name

SLC27A2 antibody - N-terminal region

Gene Names

SLC27A2; VLCS; FATP2; VLACS; ACSVL1; FACVL1; hFACVL1; HsT17226

Reactivity

Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit

Applications

IHC, WB

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)

IP (Immunoprecipitation) (Immunoprecipitation: 10ug of was used to immunoprecipitate from 500ug of Jurkat whole cell lysate. The antibody was collected on Protein A beads and eluted with sample buffer. 5uL of Jurkat whole cell lysate was then resolved via SDS-PAGE, transferred to PVDF and probed with 0.5ug . Proteins were visualized using anti-rabbit-HRP conjugate and an ECL system.)

ICC (Immunocytochemistry) (Immunocytochemistry: PI3-Kinase, p110b staining of human skeletal muscle. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern of cross banding striated muscle fibers.)

ICC (Immunocytochemistry) (Immunocytochemistry: PI3-Kinase, p110b staining in colorectal carcinoma. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a plasma membrane staining pattern.)

ICC (Immunocytochemistry) (Confocal Immunocytochemistry Analysis: HeLa cells were fixed, permeablized, and stained with (Cy3, red), DAPI (blue, nuclei), and Phalloidin-AlexaFluor488 (actin, green). Figure on the left has the DAPI filter off.)

ICC (Immunocytochemistry) (Immunocytochemistry: PI3-Kinase, p110b staining of human kidney 2 mm array spot. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is see as a staining to include the thin and distal microtubules.)

Compare (5 max)

Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26895)

Full Name

Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol

Gene Names

PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha

Reactivity

Human

Applications

Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry

Purity

Purified by Immunoaffinity chromatography.

Pricing

$1,230/0.1 mL $2,380/2x0.1 mL $3,460/3x0.1 mL $4,480/4x0.1 mL $5,260/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using Vimentin Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using Vimentin Rabbit pAb at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat uterus using Vimentin Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using Vimentin Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using Vimentin Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using Vimentin Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using Vimentin Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using Vimentin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

Compare (5 max)

Vimentin, Polyclonal Antibody (Cat# AAA28432)

Full Name

Vimentin Rabbit pAb

Gene Names

ABCA12; LI2; ICR2B; ARCI4A; ARCI4B

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using NF-kB p65 Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using NF-kB p65 Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using NF-kB p65 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using NF-kB p65 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using NF-kB p65 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using NF-kB p65 Rabbit pAb.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NF-kB p65 antibody.)

Compare (5 max)

NF-kB p65, Polyclonal Antibody (Cat# AAA30722)

Full Name

NF-kB p65 Rabbit Polyclonal Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

Survivin, Polyclonal Antibody (Cat# AAA29126)

Full Name

Survivin Polyclonal Antibody

Gene Names

BIRC5; API4; EPR-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

Application Data

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cells, using CYCS antibody.)

Compare (5 max)

AcCoA Carboxylase, Polyclonal Antibody (Cat# AAA26877)

Full Name

AcCoA Carboxylase (Ser79), Phosphorylated (ACC, Acetyl Coenzyme A Carboxylase) (MaxLight 405)

Gene Names

ACACA; ACC; ACAC; ACC1; ACCA; ACACAD

Reactivity

Mouse, Human

Applications

WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,175/0.1 mL $2,260/2x0.1 mL $3,290/3x0.1 mL $4,265/4x0.1 mL $5,000/5x0.1 mL

< >

<< Previous Next >>

IP (Immunoprecipitation) (Immunoprecipitating GARS in Hela whole cell lysateLane 1: Rabbit monoclonal IgG (1ug)instead of AAA26911 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: AAA26911 (8ug)+ Hela whole cell lysate (500ug)Lane 3: Hela whole cell lysate (20ug))

IF (Immunofluorescence) (Immunofluorescent analysis of Hela cells using AAA26911 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using AAA26911 at dilution of 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta using AAA26911 at dilution 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung tissue using AAA26911 at dilution 1:100)

WB (Western Blot) (Western BlotPositive WB detected in:Hela whole cell lysate,K562 whole cell lysate,NIH/3T3 whole cell lysate,A549 whole cell lysate,PC3 whole cell lysate,U251 whole cell lysate,Mouse lung tissue,Mouse liver tissue,Mouse kidney tissueAll lanes: ANXA2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 39,41 kDaObserved band size: 39 kDa)

WB (Western Blot) (Western blotAll lanes: Annexin A2 antibody at 2ug/ml+Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 39,41 kDaObserved band size: 39 kDa)

Compare (5 max)

Annexin A2, Polyclonal Antibody (Cat# AAA26911)

Full Name

Rabbit anti-human Annexin A2 polyclonal Antibody

Gene Names

ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Caprylic Acid Ammonium Sulfate Precipitation purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

Application Data (Human alpha-2 Macroglobulin detected with Sheep anti alpha-2 Macroglobulin:HRP)

Compare (5 max)

ALPHA 2 MACROGLOBULIN, Polyclonal Antibody (Cat# AAA12240)

Full Name

SHEEP ANTI HUMAN ALPHA 2 MACROGLOBULIN

Gene Names

A2M; A2MD; CPAMD5; FWP007; S863-7

Applications

Immunohistochemistry

Pricing

$440/1 mL $840/2x1 mL $1,205/3x1 mL $1,555/4x1 mL $1,680/5x1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat brain using RAB11ARAB11B Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using RAB11ARAB11B Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using RAB11ARAB11B Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human brain using RAB11ARAB11B Rabbit pAb.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using RAB11ARAB11B antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using RAB11ARAB11B antibody.)

Compare (5 max)

RAB11A/RAB11B, Polyclonal Antibody (Cat# AAA30731)

Full Name

RAB11A/RAB11B Rabbit Polyclonal Antibody

Gene Names

RAB11A; YL8

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

Application Data (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

Application Data (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

Compare (5 max)

NOS2, Polyclonal Antibody (Cat# AAA29081)

Full Name

NOS2 Polyclonal Antibody

Gene Names

NOS2; NOS; INOS; NOS2A; HEP-NOS

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.