FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-CD11a research biosimilar antibody Efalizumab (hu1124, AAA72240) Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of Efalizumab (AAA72240-23.0, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)
Mouse anti-Human CD11a Antibody | anti-CD11a antibody
Anti-CD11a [hu1124 (Efalizumab)], Mouse IgG2a
Gene Names
ITGAL; CD11A; LFA-1; LFA1A
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A affinity purified
Synonyms
CD11a; N/A; Anti-CD11a [hu1124 (Efalizumab)], Mouse IgG2a; Anti-CD11a [hu1124 (Efalizumab)]; LFA-1; integrin alpha L; anti-CD11a antibody
Host
Mouse
Reactivity
Human
Isotype
Mouse IgG2a, Kappa
Clone Number
hu1124 (Efalizumab)
Specificity
Binds to human LFA-1.
Purity/Purification
Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD11a antibody
FCM/FACS (Flow Cytometry), Blocking
Immunogen
Efalizumab binds to CD11a, a subunit of leukocyte function antigen-1 (LFA-1), which is expressed on all leukocytes.
Chimeric Use Statement
This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-CD11a research biosimilar antibody Efalizumab (hu1124, AAA72240) Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of Efalizumab (AAA72240-23.0, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)
FCM/FACS (Flow Cytometry)
(Flow-cytometry using the anti-CD11a research biosimilar antibody Efalizumab (hu1124, AAA72240) Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of Efalizumab (AAA72240-23.0, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody (ab150073) and cells analysed on a FACSCanto flow-cytometer.)
IHC (Immunohistochemistry)
(Immunohistochemical staining of rat spleen tissue using anti-CD11a antibody (AAA72240) hu1124 (Efalizumab). Anti-CD11a staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of hu1124 (AAA72240-10.0) was used to stain samples at a concentration of 3 ug/ml.)
IHC (Immunohistochemistry)
(Immunohistochemical staining of rat spleen tissue using anti-CD11a antibody (AAA72240) hu1124 (Efalizumab). Anti-CD11a staining of formaldehyde fixed paraffin embedded rat spleen tissue, at 40x magnification. The human IgG1-chimeric version of hu1124 (AAA72240-10.0) was used to stain samples at a concentration of 3 ug/ml.)
Related Product Information for anti-CD11a antibody
Efalizumab binds to the LFA-1 alpha I domain blocking the ICAM-1 binding site via steric hindrance.
Product Categories/Family for anti-CD11a antibody
References
Bauer et al. Population pharmacokinetics and pharmacodynamics of the anti-CD11a antibody hu1124 in human subjects with psoriasis. J Pharmacokinet Biopharm. 1999 Aug;27(4):397-420. PMID:10826130
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Integrin alpha-L
NCBI Official Synonym Full Names
integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
NCBI Official Symbol
ITGAL
NCBI Official Synonym Symbols
CD11A; LFA-1; LFA1A
NCBI Protein Information
integrin alpha-L; LFA-1A; LFA-1 alpha; integrin gene promoter; CD11 antigen-like family member A; lymphocyte function-associated antigen 1; leukocyte adhesion glycoprotein LFA-1 alpha chain; leukocyte function-associated molecule 1 alpha chain; antigen CD
UniProt Protein Name
Integrin alpha-L
UniProt Gene Name
ITGAL
UniProt Synonym Gene Names
CD11A; LFA-1A
UniProt Entry Name
ITAL_HUMAN
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Product Notes
The CD11a itgal (Catalog #AAA72240) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD11a [hu1124 (Efalizumab)], Mouse IgG2a reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD11a can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), Blocking. Researchers should empirically determine the suitability of the CD11a itgal for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD11a, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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