IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MGMT antibody. Counter stained with hematoxylin.)
Rabbit anti-Human MGMT Monoclonal Antibody | anti-MGMT antibody
MGMT Antibody
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
ProA affinity purified
Synonyms
MGMT, Antibody; MGMT Antibody; 6 O methylguanine DNA methyltransferase antibody; 6-O-methylguanine-DNA methyltransferase antibody; Agat antibody; AGT antibody; AI267024 antibody; EC 2.1.1.63 antibody; Methylated DNA protein cysteine methyltransferase antibody; Methylated-DNA--protein-cysteine methyltransferase antibody; Methylguanine DNA methyltransferase antibody; MGC107020 antibody; MGMT antibody; MGMT_HUMAN antibody; O 6 methylguanine DNA alkyltransferase antibody; O 6 methylguanine DNA methyltransferase antibody; O-6-methylguanine-DNA methyltransferase antibody; O-6-methylguanine-DNA-alkyltransferase antibody; anti-MGMT antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Clone Number
JJ089-6
Purity/Purification
ProA affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-MGMT antibody
IHC (Immunohistochemistry), WB (Western Blot)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MGMT antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemisry)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MGMT antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGMT antibody. Counter stained with hematoxylin.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MGMT antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of MGMT on different lysates using anti-MGMT antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Hela)
WB (Western Blot)
(Western blot analysis of MGMT on different lysates using anti-MGMT antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Hela)
Related Product Information for anti-MGMT antibody
MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation, only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, high MGMT activity was associated with the presence of mutant p53, in a study of oral cancer cell lines. Similarly, MGMT activity was significantly lower in ovarian tumors with wildtype p53 than in tumors with mutant p53, supporting the view that wildtype p53 down-regulates the basal MGMT promoter.
Product Categories/Family for anti-MGMT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
21,646 Da
NCBI Official Full Name
Methylated-DNA--protein-cysteine methyltransferase
NCBI Official Synonym Full Names
O-6-methylguanine-DNA methyltransferase
NCBI Official Symbol
MGMT
NCBI Protein Information
methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase
UniProt Protein Name
Methylated-DNA--protein-cysteine methyltransferase
UniProt Gene Name
MGMT
UniProt Synonym Gene Names
MGMT
UniProt Entry Name
MGMT_HUMAN
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Product Notes
The MGMT mgmt (Catalog #AAA312381) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MGMT Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MGMT can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MGMT mgmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MGMT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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