anti-Human MGMT Polyclonal Antibody | anti-MGMT antibody

Anti-MGMT Antibody

Cat. Num. AAA46549

• Reactivity: Human
• Applications: Immunocytochemistry, Immunohistochemistry, Western Blot
• Purity: Immunogen Affinity Purified
• Synonyms: MGMT, Antibody; Anti-MGMT Antibody; Methylated-DNA--protein-cysteine methyltransferase ; 6 O methylguanine DNA methyltransferase; 6-O-methylguanine-DNA methyltransferase; Agat; AGT; AI267024; Methylated DNA protein cysteine methyltransferase; Methylated-DNA--protein-cysteine methyltransferase; Methylguanine DNA methyltransferase; MGC107020; MGMT; MGMT_HUMAN; O 6 methylguanine DNA alkyltransferase; O 6 methylguanine DNA methyltransferase; O-6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase; anti-MGMT antibody

• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 207

• Applicable Applications for anti-MGMT antibody: ICC (Immunocytochemistry), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived human MGMT recombinant protein (Position: D2-N207). Human MGMT shares 70% and 69% amino acid (aa) sequence identity with mouse and rat MGMT, respectively.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

IHC (Immunohistochemisry)

(Anti- MGMT Picoband antibody, AAA46549, IHC(P)IHC(P): Human Placenta Tissue)

WB (Western Blot)

(Anti- MGMT Picoband antibody, AAA46549, Western blottingAll lanes: Anti MGMT (AAA46549) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: Human Placenta Tissue Lysate at 50ugLane 3: JURKAT Whole Cell Lysate at 40ugPredicted bind size: 21KDObserved bind size: 21KD)

WB (Western Blot)

(Anti- MGMT Picoband antibody, AAA46549, Western blottingAll lanes: Anti MGMT (AAA46549) at 0.5ug/mlWB: Recombinant Human MGMT Protein 0.5ngPredicted bind size: 21KDObserved bind size: 21KD)

Related Product Information for anti-MGMT antibody

Description: Rabbit IgG polyclonal antibody for Methylated-DNA--protein-cysteine methyltransferase(MGMT) detection. Tested with WB, IHC-P, ICC in Human.

Background: O6-alkylguanine DNA alkyltransferase (also known as AGT, MGMT or AGAT) is a protein that in humans is encoded by the O6-methylguanine DNA methyltransferase (MGMT) gene. MGMT is mapped to 10q26.3. This gene is crucial for genome stability. It repairs the naturally occurring mutagenic DNA lesion O6-methylguanine back to guanine and prevents mismatch and errors during DNA replication and transcription. It has been found that methylation of the MGMT promoter in the tumor was associated with longer survival.

References

1. Hegi, M. E., Diserens, A.-C., Godard, S., Dietrich, P.-Y., Regli, L., Ostermann, S., Otten, P., Van Melle, G., de Tribolet, N., Stupp, R. Clinical trial substantiates the predictive value of O-6-methylguanine-DNA methyltransferase promoter methylation in glioblastoma patients treated with temozolomide. Clin. Cancer Res. 10: 1871-1874, 2004. 2. Natarajan AT, Vermeulen S, Darroudi F, Valentine MB, Brent TP, Mitra S, Tano K (January 1992). "Chromosomal localization of human O6-methylguanine-DNA methyltransferase (MGMT) gene by in situ hybridization". Mutagenesis 7 (1): 83-5. 3. Shiraishi A, Sakumi K, Sekiguchi M (October 2000). "Increased susceptibility to chemotherapeutic alkylating agents of mice deficient in DNA repair methyltransferase". Carcinogenesis 21 (10): 1879-1883.

NCBI and Uniprot Product Information

• NCBI GI #: 530394832
• NCBI GeneID: 4255
• NCBI Accession #: XP_005252739.1
• NCBI GenBank Nucleotide #: XM_005252682.2
• UniProt Accession #: P16455; Q5VY78
• Molecular Weight: 21,646 Da
• NCBI Official Full Name: methylated-DNA--protein-cysteine methyltransferase isoform X2
• NCBI Official Synonym Full Names: O-6-methylguanine-DNA methyltransferase
• NCBI Official Symbol: MGMT
• NCBI Protein Information: methylated-DNA--protein-cysteine methyltransferase
• UniProt Protein Name: Methylated-DNA--protein-cysteine methyltransferase
• UniProt Gene Name: MGMT
• UniProt Synonym Gene Names: MGMT
• UniProt Entry Name: MGMT_HUMAN