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IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue.1,CA IX Monoclonal Antibody(12F10) was diluted at 1:200(4C,overnight).2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min).3,Secondary antibody was diluted at 1:200(room temperature, 30min).Negative control was used by secondary antibody only.)
Mouse anti-Human Carbonic Anhydrase IX Monoclonal Antibody | anti-CA9 antibody
Carbonic Anhydrase IX Mouse Monoclonal Antibody
Gene Names
CA9; MN; CAIX
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity purification using immunogen.
Synonyms
Carbonic Anhydrase IX, Antibody; Carbonic Anhydrase IX Mouse Monoclonal Antibody; CA-IX; CA9; CAH9; CAIX; Car9; Carbonate dehydratase IX; Carbonic anhydrase 9; anti-CA9 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Specificity
CA IX Mouse Monoclonal antibody detects endogenous CA IX protein.s
Purity/Purification
Affinity purification using immunogen.
Form/Format
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for anti-CA9 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Target Name
Carbonic Anhydrase IX
Preparation and Storage
Store at -20°C.
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue.1,CA IX Monoclonal Antibody(12F10) was diluted at 1:200(4C,overnight).2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min).3,Secondary antibody was diluted at 1:200(room temperature, 30min).Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue.1,CA IX Monoclonal Antibody(12F10) was diluted at 1:200(4C,overnight).2, Sodium citrate pH 6.0 was used for antibody retrieval(>98C,20min).3,Secondary antibody was diluted at 1:200(room temperature, 30min).Negative control was used by secondary antibody only.)
IF (Immunofluorescence)
(Immunofluorescence analysis of human-liver-cancer tissue.1,CA IX Monoclonal Antibody(12F10)(red) was diluted at 1:200(4C,overnight).2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min.Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence)
(Immunofluorescence analysis of human-liver-cancer tissue.1,CA IX Monoclonal Antibody(12F10)(red) was diluted at 1:200(4C,overnight).2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min.Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
WB (Western Blot)
(1B'B'Input: Hela Cell Lysate2B'B'IP product: IP dilute 1:200Western blot analysis: primary antibody : AAA300784 1:2,000Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000)
WB (Western Blot)
(1B'B'Input: Hela Cell Lysate2B'B'IP product: IP dilute 1:200Western blot analysis: primary antibody : AAA300784 1:2,000Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000)
WB (Western Blot)
(Western blot analysis of 1) Hela, 2) 293T, using AAA300784 diluted at 1:5,000.)
WB (Western Blot)
(Western blot analysis of 1) Hela, 2) 293T, using AAA300784 diluted at 1:5,000.)
Related Product Information for anti-CA9 antibody
The carbonic anhydrases (or carbonate dehydratases) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. CAIX is considered to be one of the best cellular biomarkers of hypoxia. Furthermore, recent studies examining the association between CAIX levels and various clinicopathological outcomes suggest that CAIX expression may also be a valuable prognostic indicator for overall survival. Antibodies against CAIX serve as excellent excellent biomarkers of hypoxic regions in many solid tumors.
Product Categories/Family for anti-CA9 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
SDS-PAGE MW: 49kd
NCBI Official Full Name
carbonic anhydrase 9
NCBI Official Synonym Full Names
carbonic anhydrase IX
NCBI Official Symbol
CA9
NCBI Official Synonym Symbols
MN; CAIX
NCBI Protein Information
carbonic anhydrase 9
UniProt Protein Name
Carbonic anhydrase 9
UniProt Gene Name
CA9
UniProt Synonym Gene Names
G250; MN; CA-IX; CAIX; RCC-associated antigen G250
UniProt Entry Name
CAH9_HUMAN
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Product Notes
The CA9 ca9 (Catalog #AAA300784) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Carbonic Anhydrase IX Mouse Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Carbonic Anhydrase IX can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the CA9 ca9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase IX, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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