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product-image-AAA72298_IHC15.png IHC (Immunohistochemistry) (Immunohistochemical staining of human MCF-7 tumour cell spheroid sections using anti-CAIX antibody M75. Anti-CAIX staining of formaldehyde fixed paraffin embedded human MCF-7 tumour cell spheroid sections, at 40x magnification. The mouse IgG1-chimeric version of M75 (AAA72298) successfully detected CAIX.)

anti-Human Carbonic Anhydrase IX Antibody | anti-CA9 antibody

Anti-Human Carbonic Anhydrase IX [M75]

Gene Names
CA9; MN; CAIX
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified. Protein A affinity purified
Synonyms
Carbonic Anhydrase IX; N/A; Anti-Human Carbonic Anhydrase IX [M75]; CA9; anti-CA9 antibody
Ordering
Reactivity
Human
Isotype
Mouse IgG1 kappa
Clone Number
M75
Specificity
This antibody binds to the epitope APGEEDLPA of human carbonic anhydrase IX.
Purity/Purification
Purified. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CA9 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Pub PMID
18041760
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

IHC (Immunohistochemistry)

(Immunohistochemical staining of human MCF-7 tumour cell spheroid sections using anti-CAIX antibody M75. Anti-CAIX staining of formaldehyde fixed paraffin embedded human MCF-7 tumour cell spheroid sections, at 40x magnification. The mouse IgG1-chimeric version of M75 (AAA72298) successfully detected CAIX.)

product-image-AAA72298_IHC15.png IHC (Immunohistochemistry) (Immunohistochemical staining of human MCF-7 tumour cell spheroid sections using anti-CAIX antibody M75. Anti-CAIX staining of formaldehyde fixed paraffin embedded human MCF-7 tumour cell spheroid sections, at 40x magnification. The mouse IgG1-chimeric version of M75 (AAA72298) successfully detected CAIX.)
Related Product Information for anti-CA9 antibody
MaTu-infected HeLa cells (MaTu=Mammary Tumour)
Describes how the MaTu antigen acts as a quasi-viral agent and the generation of monoclonal antibodies against it.
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.
This antibody binds to human carbonic anhydrase IX, which reversibly hydrates carbon dioxide and participes in pH regulation. It is involved in control of cell proliferation and can contribute towards transformation. CA9 has recently been identified as a biomarker for cervical neoplasia (PubMed: 19670419).
Product Categories/Family for anti-CA9 antibody
References
Pastorekova et al.
Virology 1992; 187(2):620-626

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
768
UniProt Accession #
Molecular Weight
49,698 Da
NCBI Official Full Name
carbonic anhydrase IX
NCBI Official Synonym Full Names
carbonic anhydrase 9
NCBI Official Symbol
CA9
NCBI Official Synonym Symbols
MN; CAIX
NCBI Protein Information
carbonic anhydrase 9
UniProt Protein Name
Carbonic anhydrase 9
UniProt Gene Name
CA9
UniProt Synonym Gene Names
G250; MN; CA-IX; CAIX; RCC-associated antigen G250

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Product Notes

The CA9 ca9 (Catalog #AAA72298) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human Carbonic Anhydrase IX [M75] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Carbonic Anhydrase IX can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the CA9 ca9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carbonic Anhydrase IX, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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