Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (CPSF3 monoclonal antibody. Western Blot analysis of CPSF3 expression in NIH/3T3.)
WB (Western Blot) (Western Blot analysis of CPSF3 expression in transfected 293T cell line by CPSF3 monoclonal antibody. Lane 1: CPSF3 transfected lysate (77.5kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged CPSF3 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CPSF3 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (CPSF3 monoclonal antibody, Western Blot analysis of CPSF3 expression in Hela NE.)
WB (Western Blot) (CPSF3 monoclonal antibody. Western Blot analysis of CPSF3 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (G3BP monoclonal antibody Western Blot analysis of G3BP expression in A-431)
WB (Western Blot) (Western blot analysis of G3BP over-expressed 293 cell line, cotransfected with G3BP Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with G3BP monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to G3BP on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to G3BP on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 1ug/ml])
WB (Western Blot) (Western Blot analysis of G3BP expression in transfected 293T cell line by G3BP monoclonal antibody Lane 1: G3BP transfected lysate (52.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
G3BP1 (Ras GTPase-activating Protein-binding Protein 1, G3BP-1, ATP-dependent DNA Helicase VIII, hDH VIII, GAP SH3 Domain-binding Protein 1, G3BP) (PE)
Gene Names
G3BP1; G3BP; HDH-VIII
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Application Data (Detection limit for recombinant GST tagged NME1 is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (NME1 monoclonal antibody (M02), clone 1D7. Western Blot analysis of NME1 expression in different cell lines.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NME1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NME1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
ELISA (ELISA plate was coated by 2019-nCoV NP, 100 uL/cell at 5ug/ml. The indirect ELISA analysis was performed by loading 100 uL per well of the anti-2019-nCoV N mAb (BN9) at various concentrations. The plate was incubated for 1 hours at 37 degree C, then washed 5 times. An anti hFc HRP conjugated mAb at a concentration of 1:2000, 100uL/well was used as the secondary antibody. Again, the plate was incubated for 1 hours at 37 degree C, then washed 5 times. Detection was performed using TMB substrate for 10 minutes at room temperature in the dark. The plate was stopped with 2M sulfuric acid. Signal was read on a spectrophotometer at 450 nm.)
ICC (Immunocytochemistry) (Confocal imaging of human liver using Ceruloplasmin Rabbit mAb (AAA28540,at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.)
IF (Immunofluorescence) (Immunofluorescence analysis of paraffin-embedded mouse liver using Ceruloplasmin Rabbit mAb (AAA28540) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of paraffin-embedded rat liver using Ceruloplasmin Rabbit mAb (AAA28540) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Ceruloplasmin Rabbit mAb (AAA28540) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Ceruloplasmin Rabbit mAb (AAA28540) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using Ceruloplasmin Rabbit mAb (AAA28540) at a dilution of 1:10000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using Ceruloplasmin Rabbit mAb (AAA28540) at 1:5000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
ICC (Immunocytochemistry) (ICC staining MSH6 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MSH6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-MSH6 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-MSH6 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MSH6 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MSH6 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of MSH6 on different lysates using anti-MSH6 antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: SW480 Lane 3: A549)
IF (Immunofluorescence) (Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red))
FCM (Flow Cytometry) (Flow Cytometric Analysis of T98G cells using Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)
WB (Western Blot) (Western Blot Analysis of human brain tissue lysate using Pgp9.5 / UchL1 Mouse Monoclonal Antibody (31A3).)
WB (Western Blot) (Western Blot of UchL1 (PGP9.5) in 1) human, 2) mouse and 3) rat brain Lysate using UchL1 Monoclonal Antibody (31A3).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Cerebellum stained with Pgp9.5 / UchL1 Monoclonal Antibody (31A3).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using NR6A1 mouse mAb with DAB staining.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded cerebellum tissues using NR6A1 mouse mAb with DAB staining.)
FCM (Flow Cytometry) (Flow cytometric analysis of K562 cells using NR6A1 mouse mAb (green) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells using NR6A1 mouse mAb (green). Blue)
WB (Western Blot) (Western blot analysis using NR6A1 mouse mAb against K562 (1), NTERA-2 (2), HEK293 (3), HUVE-12 (4), and HeLa (5) cell lysate.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-GCNF mouse mAb (dilution 1:200).)
WB (Western Blot) (Western blot detection of GCNF in Jurkat, MCF7 and Hela cell lysates using GCNF mouse mAb (1:3000 diluted).Predicted band size:54KDa.Observed band size:54KDa.)
WB (Western Blot) (NADK monoclonal antibody. Western Blot analysis of NADK expression in HepG2.)
WB (Western Blot) (NADK monoclonal antibody, Western Blot analysis of NADK expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged NADK is ~0.3ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of NADK expression in transfected 293T cell line by NADK monoclonal antibody. Lane 1: NADK transfected lysate (49.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NADK monoclonal antibody. Western Blot analysis of NADK expression in NIH/3T3.)
WB (Western Blot) (NADK monoclonal antibody. Western Blot analysis of NADK expression in Raw 264.7.)
WB (Western Blot) (Western Blot detection against Immunogen (75.17kD).)
WB (Western Blot) (ACBD3 monoclonal antibody. Western Blot analysis of ACBD3 expression in PC-12.)
WB (Western Blot) (ACBD3 monoclonal antibody, Western Blot analysis of ACBD3 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged ACBD3 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACBD3 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACBD3 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ACBD3 expression in transfected 293T cell line by ACBD3 monoclonal antibody. Lane 1: ACBD3 transfected lysate (60.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
ACBD3 (Golgi Resident Protein GCP60, Acyl-CoA-binding Domain-containing Protein 3, Golgi Complex-associated Protein 1, GOCAP1, Golgi Phosphoprotein 1, GOLPH1, PBR- and PKA-associated Protein 7, Peripheral Benzodiazepine Receptor-associated Protein PAP7, G
Gene Names
ACBD3; PAP7; GCP60; GOCAP1; GOLPH1
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
WB (Western Blot) (Western blot analysis of GABBR1 over-expressed 293 cell line, cotransfected with GABBR1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GABBR1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged GABBR1 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABBR1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of GABBR1 expression in transfected 293T cell line by GABBR1 monoclonal antibody. Lane 1: GABBR1 transfected lysate (95kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (GABBR1 monoclonal antibody, Western Blot analysis of GABBR1 expression in IMR-32.)
WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)
WB (Western Blot) (AKR1B10 monoclonal antibody, Western Blot analysis of AKR1B10 expression in HepG2.)
Application Data (Detection limit for recombinant GST tagged AKR1B10 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKR1B10 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AKR1B10 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of AKR1B10 expression in transfected 293T cell line by AKR1B10 monoclonal antibody. Lane 1: AKR1B10 transfected lysate (36kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (33.59kD).)
WB (Western Blot) (Detection of human SMAD2 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, K-562, A-549, RKO, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMAD2 recombinant monoclonal antibody (AAA23843 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot) (Detection of mouse SMAD2 by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3, mIMCD-3, A20, NIH 3T3, CT26, EL4, RenCa, F9, C2C12, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMAD2 recombinant monoclonal antibody (AAA23843 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation) (Detection of human SMAD2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMAD2 recombinant monoclonal antibody (AAA23843 lot 1) used for IP at 12 ul per reaction. SMAD2 was also immunoprecipitated by rabbit anti-SMAD2 antibody BL1658B-3F10. For blotting immunoprecipitated SMAD2, AAA23843 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IHC (Immunohistochemistry) (Detection of human SMAD2 in FFPE lung carcinoma by immunohistochemistry. Antibody: Rabbit anti-SMAD2 recombinant monoclonal antibody (AAA23843 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human SMAD2 in FFPE A549 cells by immunocytochemistry. Antibody: Rabbit anti-SMAD2 recombinant monoclonal antibody (AAA23843 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human Smad2 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-Smad2 recombinant monoclonal antibody (AAA23843 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (Western blot analysis of ORM1 over-expressed 293 cell line, cotransfected with ORM1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ORM1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged ORM1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ORM1 transfected lysate using ORM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ORM1 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ORM1 on formalin-fixed paraffin-embedded human stomach carcinoma tissue. [antibody concentration 1ug/ml])
WB (Western Blot) (Western Blot analysis of ORM1 expression in transfected 293T cell line by ORM1 monoclonal antibody. Lane 1: ORM1 transfected lysate (23.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ORM1 monoclonal antibody Western Blot analysis of ORM1 expression in MCF-7.)
WB (Western Blot) (Western Blot detection against Immunogen (45.98kD).)
Application Data (Detection limit for recombinant GST tagged PCNA is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PCNA transfected lysate using PCNA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PCNA rabbit polyclonal antibody)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PCNA on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody Lane 1: PCNA transfected lysate (28.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PCNA monoclonal antibody Western Blot analysis of PCNA expression in Hela.)
WB (Western Blot) (Western Blot detection against Immunogen (54.45kD).)
ICC (Immunocytochemistry) (ICC staining Phospho-eIF4E (S209) in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-eIF4E (S209) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-eIF4E (S209) in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Phospho-eIF4E (S209) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-Phospho-eIF4E (S209) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Phospho-eIF4E (S209) antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Phospho-eIF4E (S209) on different lysates using anti-Phospho-eIF4E (S209) antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse spleen Lane 2: Rat spleen)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Peroxiredoxin 2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Peroxiredoxin 2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Peroxiredoxin 2 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Peroxiredoxin 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Peroxiredoxin 2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Peroxiredoxin 2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Peroxiredoxin 2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Peroxiredoxin 2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Peroxiredoxin 2 on different lysates using anti-Peroxiredoxin 2 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-S100A10 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-S100A10 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-S100A10 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-S100A10 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-S100A10 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of S100A10 on human lung lysates using anti-S100A10 antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-CDC5L mAb. [Lot No. 2136C1a-1])
FCM (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-CDC5L mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2136C1a-1])
IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-CDC5L mAb. [Lot No. 2136C1a-1]Predicted molecular weight: 92 kDa)
WB (Western Blot) (Detection of CDC5L by Western blot.Samples: Whole cell lysate from human HeLa (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. 2136C1a-1]Predicted molecular weight: 92 kDa)
Quality Control (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-CDC5L monoclonal antibody.)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD13: Alexa Fluor 647 (AAA11927A647))
Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 (red in A) and Mouse anti CD21 antibody, clone LB21 (green in B). The merged image is in C with nuclei counterstained blue using DAPI. High power.)
Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD13: APC)
Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 (red in A) and Mouse anti CD21 antibody, clone LB21 (green in B). The merged image is in C with nuclei counterstained blue using DAPI. Medium power.)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD13)
Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 (red in A) and Mouse anti CD21 antibody, clone LB21 (green in B). The merged image is in C with nuclei counterstained blue using DAPI. Low power.)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 followed by Histar Detection System. Medium power)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD13:RPE)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD13:FITC)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 followed by Histar Detection System. Medium power)
Application Data (Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 488 (AAA11941A488))
Application Data (Published customer image: Blockade of the mevalonate pathway increases CD9 and CD81. (A) RAW264.7 cells were untreated (-) or treated for 48 h with 50 ng/ml TSA (+) in the absence (-) or presence of 50 uM theophylline or 0.5 uM fluvastatin (Fluv) (+). The cells were lysed, and levels of CD9 and CD81 were examined by immunoblotting. Anti-actin blots show that comparable amounts of protein were loaded in each lane. (B) The mevalonate pathway and inhibitors. n-BP, nitrogenous bisphosphonate. (C) RAW264.7 cells were cultured for 24 h in the presence of indicated concentrations of fluvastatin, simvastatin (Simv), zoledronate (Zol), or risedronate (Ris). Levels of CD9 and CD81 were examined by immunoblotting. (D) RAW264.7 cells were cultured for 24 h in the absence (V, vehicle alone) or presence of mevalonate (Mev), farnesyl pyrophosphate (FPP), squalene (Squ), or geranylgeranyl pyrophosphate (GGPP). Although the actin level in the GGPP lane appears to be lower, an equal amount of protein was loaded. (E) RAW264.7 cells were cultured for 24 h in the absence (V) or presence of fluvastatin, zoledronate, farnesyl transferase inhibitor (FTI), or geranylgeranyl transferase inhibitor (GGTI). (F) RAW264.7 cells were untreated (-) or treated with fluvastatin (+) in the absence (V) or presence of mevalonate, FPP, squalene, or GGPP. (G) RAW264.7 cells were untreated (-) or treated with zoledronate (+) in the absence (V) or presence of mevalonate, FPP, squalene, or GGPP. (H) RAW264.7 cells were untreated (-) or treated with fluvastatin (+) in the absence (V) or presence of mevalonate, FPP, squalene, or GGPP and stimulated for 15 min with 0.1 ug/ml LPS (+). The cells were lysed, and levels of I?Ba were examined by immunoblotting. (I) RAW264.7 cells were cultured for 24 h in the indicated concentrations of HA1077. Levels of CD9 and CD81 were examined by immunoblotting.From: Jin Y, Tachibana I, Takeda Y, He P, Kang S, et al. (2013) Statins Decrease Lung Inflammation in Mice by Upregulating Tetraspanin CD9 in Macrophages. PLoS ONE 8(9): e73706.)
Application Data (Published customer image: Screening of a drug library for agents that upregulate CD9 or CD81 in RAW264.7 macrophages. (A) RAW264.7 cells were cultured for 24 h in the absence (V, vehicle alone) and presence of each drug (10 uM). The cells were lysed, and levels of CD9 and CD81 were examined by immunoblotting. Blots of results with fluvastatin (Fluv) and simvastatin (Simv) are shown. Anti-actin blots show that comparable amounts of protein were loaded in each lane. (B) After testing 1,165 drugs, levels of CD9 and CD81 relative to actin were quantified by densitometry. Fold changes of the expression levels compared with vehicle alone were calculated and plotted. Drugs that increased the level of either CD9 or CD81 more than 1.5-fold compared with vehicle alone were regarded as positive. Correlation between fold changes in CD9 and CD81 levels was analyzed using Pearson's correlation coefficient. (C) RAW264.7 cells were cultured in the absence (V) or presence of multiple statins (10 uM) and levels of CD9 and CD81 were examined by immunoblotting. The statins are arranged in order of decreasing lipophilicity. Ceri, cerivastatin; Simv, simvastatin; Fluv, fluvastatin; Ator, atorvastatin; Rosu, rosuvastatin; Prav, pravastatin. (D) RAW264.7 cells were cultured in the absence (shaded histograms) or presence (10 uM) of fluvastatin (open red histograms) and simvastatin (open blue histograms). Surface levels of CD9, CD63, CD81, and the integrin beta1 subunit were analyzed by flow cytometry.From: Jin Y, Tachibana I, Takeda Y, He P, Kang S, et al. (2013) Statins Decrease Lung Inflammation in Mice by Upregulating Tetraspanin CD9 in Macrophages. PLoS ONE 8(9): e73706.)
Application Data (Published customer image: Density fractionation of EVs. The figure shows sucrose gradients of EVs preparations from MLP29 (A) and RH (B). Aliquots of these fractions were used for RNA extraction and protein extraction; the most abundant transcripts were found in the fractions containing typical exosomal markers (Tsg101 or Aip1). RH preparations showed more diversity, with vesicle populations fractionating at different densities.From: Royo F, Schlangen K, Palomo L, Gonzalez E, Conde-Vancells J, et al. (2013) Transcriptome of Extracellular Vesicles Released by Hepatocytes. PLoS ONE 8(7): e68693.)
Application Data (Published customer image: The anti-inflammatory effects of statins are CD9-dependent. (A) BMDMs from WT mice were cultured for 24 h in the absence (-) or presence of 3 uM fluvastatin (Fluv) (+), and unstimulated (-) or stimulated for 24 h with 1 ug/ml LPS (+). The cells were lysed, and levels of CD9 and CD81 were examined by immunoblotting. Anti-actin blots show that comparable amounts of protein were loaded in each lane. (B) BMDMs from WT and CD9 KO mice were cultured in the absence or presence of the indicated concentrations of fluvastatin, and stimulated for 18 h with 10 ug/ml LPS (+). Activities of MMP-9 in culture supernatants were analyzed by gelatin zymography. (C) BMDMs from WT and CD9 KO mice were cultured in the absence (vehicle) or presence of 10 uM fluvastatin or simvastatin (Simv), and unstimulated (-) or stimulated for 18 h with 1 ug/ml LPS (+). Concentrations of TNF-a in culture supernatants were measured by ELISA. Each bar represents the mean +/- SEM. ?P < 0.05; ? ? P < 0.01.From: Jin Y, Tachibana I, Takeda Y, He P, Kang S, et al. (2013) Statins Decrease Lung Inflammation in Mice by Upregulating Tetraspanin CD9 in Macrophages. PLoS ONE 8(9): e73706.)
Application Data (Published customer image: Statins transfer CD14 from lipid rafts into CD9-enriched microdomains. (A) RAW264.7 cells were stimulated with 0.1 ug/ml LPS and, after the indicated times, the cells were lysed and protein levels were examined by immunoblotting. Anti-actin blots show that comparable amounts of protein were loaded in each lane. (B) RAW264.7 cells were untreated (-) or cultured for 24 h in the absence (-) or presence of 5 uM fluvastatin (Fluv) or simvastatin (Simv) (+) and stimulated for 2 h with 1 ug/ml LPS (+). Proteins in whole-cell lysate (WCL) and CD14 protein in immunoprecipitates (IP) with anti-TLR4 Ab were immunoblotted (IB). (C) RAW264.7 cells were treated as in B. Lysates of untreated (C, control) cultures or LPS-stimulated cultures in the absence (L) or presence of fluvastatin (FL) or simvastatin (SL) were fractionated by sucrose density gradients, and protein distributions were visualized by immunoblotting. The intensities of blots were quantified by densitometry, and percentages of density units of light membrane (LM) fractions are displayed to the right of the blots. Data shown are from one representative of three similar experiments. (D) Immunoblots of CD9 and CD81 proteins in whole-cell lysates and in immunoprecipitates with control IgG or anti-CD14 mAb. (E) Immunoblots of CD9 and CD81 proteins in whole-cell lysates and in immunoprecipitates with control IgG or anti-CD14 mAb from pooled LM fractions (4 and 5) and dense (D) fractions (9 and 10). In the presence of statins, more CD14/CD9 complexes were formed in dense fractions (arrowheads).From: Jin Y, Tachibana I, Takeda Y, He P, Kang S, et al. (2013) Statins Decrease Lung Inflammation in Mice by Upregulating Tetraspanin CD9 in Macrophages. PLoS ONE 8(9): e73706.)
Application Data (Published customer image: Fluvastatin and simvastatin increase CD9 and CD81 levels in RAW264.7 cells.(A) RAW264.7 cells were cultured for 24 h in the absence or presence of increasing concentrations of fluvastatin (Fluv) or simvastatin (Simv). The cells were lysed, and levels of CD9, CD63, and CD81 were examined by immunoblotting. Anti-actin blots show that comparable amounts of protein were loaded in each lane. (B) RAW264.7 cells were untreated (-) or cultured in the absence or presence of increasing concentrations of fluvastatin or simvastatin and stimulated for 24 h with 0.1 ug/ml LPS (+). Levels of CD9, CD63, and CD81 were examined by immunoblotting. Note that LPS downregulates CD9 and CD81 in the absence of statins (arrowheads). (C) RAW264.7 cells were cultured in the absence (-) or presence of 3 uM fluvastatin (+), and unstimulated (-) or stimulated for 24 h with 1 ug/ml LPS (+). mRNA levels of CD9 and CD81 were examined by reverse transcription PCR. GAPDH is an internal loading control. (D) RAW264.7 cells were cultured in the absence or presence of fluvastatin, and unstimulated or stimulated with LPS. Control (Cont) was an untreated culture. mRNA levels of CD9 and CD81 were examined by real-time PCR. Data shown are from one representative of three similar experiments. (E) Human monocytic THP-1 cells were treated for 4 h with 1 ug/ml phorbol 12-myristate 13-acetate, allowed to attach to a plate, and then cultured in the absence or presence of increasing concentrations of simvastatin. Levels of CD9, CD63, and CD81 were examined by immunoblotting. (F) Mouse 3T3 fibroblasts were cultured in the absence or presence of increasing concentrations of simvastatin. Levels of CD9, CD63, and CD81 were examined by immunoblotting.From: Jin Y, Tachibana I, Takeda Y, He P, Kang S, et al. (2013) Statins Decrease Lung Inflammation in Mice by Upregulating Tetraspanin CD9 in Macrophages. PLoS ONE 8(9): e73706.)
Application Data (Staining of mouse spleen cells with Hamster anti Mouse CD81:FITC)
Application Data (Staining of mouse spleen with Hamster anti Mouse CD81: Alexa Fluor 647 (AAA11941A647))
Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse MARCO: FITC)
Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image wiith nuclei counterstained blue using DAPI. Low power)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image wiith nuclei counterstained blue using DAPI. high power)
Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse MARCO antibody, clone ED31 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)
WB (Western Blot) (Western Blot detection against Immunogen (36.89kD).)
Application Data (Detection limit for recombinant GST tagged RICTOR is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of RICTOR transfected lysate using RICTOR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RICTOR rabbit polyclonal antibody)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RICTOR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody. Lane 1: RICTOR transfected lysate (29.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (RICTOR monoclonal antibody Western Blot analysis of RICTOR expression in Hela NE.)
FCM (Flow Cytometry) (Flow cytometric analysis of 293T cells with BRD2 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining BRD2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining BRD2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining BRD2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-BRD2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-BRD2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of BRD2 on SiHa cell lysate using anti-BRD2 antibody at 1/2, 000 dilution.)
WB (Western Blot) (Western blot analysis of TAF7 over-expressed 293 cell line, cotransfected with TAF7 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TAF7 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged TAF7 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TAF7 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of TAF7 expression in transfected 293T cell line by TAF7 monoclonal antibody. Lane 1: TAF7 transfected lysate (40.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TAF7 monoclonal antibody, Western Blot analysis of TAF7 expression in MCF-7.)
Application Data (Detection limit for recombinant GST tagged PIK3R4 is approximately 0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PIK3R4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.6 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PIK3R4 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.6 ug/ml])
WB (Western Blot) (PIK3R4 monoclonal antibody (M02), clone 1B5 Western Blot analysis of PIK3R4 expression in HeLa.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PIK3R4 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PIK3R4 on HeLa cell. [antibody concentration 10 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with ERK2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining ERK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ERK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ERK2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-ERK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-ERK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ERK2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of ERK2 on different lysates using anti-ERK2 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: PC-12)
WB (Western Blot) (DCPS monoclonal antibody. Western Blot analysis of DCPS expression in PC-12.)
Application Data (Detection limit for recombinant GST tagged DCPS is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DCPS on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DCPS on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.5ug/ml])
WB (Western Blot) (DCPS monoclonal antibody Western Blot analysis of DCPS expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (63.18kD).)
IF (Immunofluorescence) (ICC/IF analysis of FABP7 in PC3 cells. The cell was stained with AAA11731 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
IF (Immunflorescense) (ICC/IF analysis of FABP7 in U87MG cells. The cell was stained with AAA11731 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
WB (Western Blot) (The tissue lysate of mouse brain (60ug) was resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FABP7 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
WB (Western Blot) (The Mouse tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP7 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Testis tissue lysate)
WB (Western Blot) (Detection of human Phospho-MCM2 (S53) by western blot. Samples: Whole cell lysate (10 ug) from HDLM-2, HeLa, Jurkat, MCF-7, MOLT-4, Malme-3M, HEK293T, and Raji cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho-MCM2 (S53) recombinant monoclonal antibody (AAA23835 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal .)
WB (Western Blot) (Detection of human Phospho-MCM2 (S53) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-MCM2 antibody used for IP. Immunoprecipitates where either treated with phosphatase (+) or mock treated (-). For blotting immunoprecipitated Phospho-MCM2 (S53), AAA23835 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Total MCM2 .)
WB (Western Blot) (Detection of human Phospho-MCM2 (S53) by western blot. Samples: Whole cell lysate (10 ug) from BW5147.3, TCMK-1, mIMCD-3, A20, NIH 3T3, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho-MCM2 (S53) recombinant monoclonal antibody (AAA23835 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal .)
IHC (Immunohistochemistry) (Detection of mouse Phospho-MCM2 (S53) in FFPE mouse renal cell carcinoma by immunohistochemistry. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal antibody (AAA23835 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human Phospho-MCM2 (S53) in FFPE lung carcinoma by immunohistochemistry. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal antibody (AAA23835 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human Phospho-MCM2 (S53) in FFPE OVCAR-4 cells by immunocytochemistry. Antibody: Rabbit anti-MCM2 (S53) recombinant monoclonal antibody (AAA23835 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
Application Data (Detection limit for recombinant GST tagged NDUFA9 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NDUFA9 on NIH/3T3 cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NDUFA9 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.8ug/ml])
WB (Western Blot) (NDUFA9 monoclonal antibody Western Blot analysis of NDUFA9 expression in NIH/3T3.)
WB (Western Blot) (NDUFA9 monoclonal antibody. Western Blot analysis of NDUFA9 expression in Raw 264.7.)
WB (Western Blot) (NDUFA9 monoclonal antibody Western Blot analysis of NDUFA9 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (33.99kD).)
Application Data (Detection limit for recombinant GST tagged SHMT1 is ~1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 1ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot analysis of SHMT1 expression in transfected 293T cell line by SHMT1 monoclonal antibody. Lane 1: SHMT1 transfected lysate (53.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SHMT1 monoclonal antibody, Western Blot analysis of SHMT1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)
Application Data (Detection limit for recombinant GST tagged DNAJB6 is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DNAJB6 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of DNAJB6 expression in transfected 293T cell line by DNAJB6 monoclonal antibody. Lane 1: DNAJB6 transfected lysate (27KD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (DNAJB6 monoclonal antibody Western Blot analysis of DNAJB6 expression in Jurkat.)
WB (Western Blot) (DNAJB6 monoclonal antibody Western Blot analysis of DNAJB6 expression in C32.)
WB (Western Blot) (Western Blot detection against Immunogen (52.25kD).)
ICC (Immunocytochemistry) (ICC staining PRMT5 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PRMT5 on A431 cell lysates using anti-PRMT5 antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with HNF-4-alpha antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining HNF-4-alpha in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-HNF-4-alpha antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HNF-4-alpha antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HNF-4-alpha antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of HNF-4-alpha on human kidney lysates using anti-HNF-4-alpha antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western Blot analysis of TOMM22 expression in transfected 293T cell line by TOMM22 monoclonal antibody. Lane 1: TOMM22 transfected lysate (15.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TOMM22 monoclonal antibody. Western Blot analysis of TOMM22 expression in PC-12.)
WB (Western Blot) (Western blot analysis of TOMM22 over-expressed 293 cell line, cotransfected with TOMM22 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TOMM22 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged TOMM22 is 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TOMM22 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TOMM22 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot detection against Immunogen (41.73kD).)
IF (Immunofluorescence) (Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled Isotype Control Monoclonal Antibody (IgG1) (Green).DAPI was used to stain the cell nuclei (blue). (Negative Control))
IF (Immunofluorescence) (Confocal Immunofluorescent analysis of SK-OV-3 cells using AF488-labeled EpCAM Monoclonal Antibody (EGP40/837) (Green). DAPI was used to stain the cell nuclei (blue).)
WB (Western Blot) (Western Blot of HCT116 Cell Lysate using Ep-CAM Monoclonal Antibody (EGP40/837).)
Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.