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Western Blot (WB) (The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human TREM2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : 293T cell lysateLane 2 : TREM2 19-161aa Transfected 293T cell lysate)
Immunocytochemistry (ICC)/Immunofluorescence (IF) (ICC/IF analysis of TREM2 in HepG2 cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Immunocytochemistry (ICC)/Immunofluorescence (IF) (ICC/IF analysis of TREM2 in Raw264.7 cells. The cell was stained (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Flow Cytometry (FC/FACS) (Flow cytometry analysis of TREM2 in Hep3B cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).)
WB (Western Blot) (CDC2 monoclonal antibody, Western Blot analysis of CDC2 expression in Hela.)
Application Data (Detection limit for recombinant GST tagged CDC2 is ~30ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDC2 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.3ug/ml].)
WB (Western Blot) (Western Blot analysis of CDC2 expression in transfected 293T cell line by CDC2 monoclonal antibody. Lane 1: CDC2 transfected lysate (34.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (58.41kD).)
WB (Western Blot) (Detection of mouse SOX10 by western blot. Samples: Whole cell lysate from TCMK-1 (50 ug), B16-F10 (15 ug), NIH 3T3 (50 ug), and Malme-3M (15 ug) cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody (AAA23824 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)
WB (Western Blot) (Detection of human SOX10 by western blot. Samples: Whole cell lysate (15 ug) from HEK293T, Malme-3M, MCF-7, SK-MEL-28, Jurkat, MDA-MB-435, HeLa, and UACC-62 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody (AAA23824 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-GAPDH .)
IP (Immunoprecipitation) (Detection of human SOX10 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Malme-3M cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SOX10 recombinant monoclonal antibody (AAA23824 lot 1) used for IP at 20 ul/mg lysate. SOX10 was also immunoprecipitated by a rabbit anti-SOX10 antibodies . For blotting immunoprecipitated SOX10, AAA23824 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry) (Detection of human SOX10 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody (AAA23824 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human SOX10 in FFPE melanoma by IHC. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody (AAA23824 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human SOX10 in FFPE SK-MEL-28 cells by ICC. Antibody: Rabbit anti-SOX10 recombinant monoclonal antibody (AAA23824 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
WB (Western Blot) (Western blot analysis of ARNT over-expressed 293 cell line, cotransfected with ARNT Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ARNT monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western Blot analysis of ARNT expression in transfected 293T cell line by ARNT monoclonal antibody. Lane 1: ARNT transfected lysate (86.6kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged ARNT is ~0.03ng/ml as a capture antibod)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ARNT on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ARNT on formalin-fixed paraffin-embedded human lung. [antibody concentration 3ug/ml].)
WB (Western Blot) (ARNT monoclonal antibody Western Blot analysis of ARNT expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)
IP (Immunoprecipitation) (CD147 was immunoprecipitated using:Lane A:0.5 mg K562 Whole Cell Lysate2 uL anti-CD147 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD147 rabbit monoclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 42 kDaObserved band size: 50 kDa)
Application Data (Analysis of anti-CD147 reactivity on HeLa cells.)
IHC (Immunohistochemistry) (Immunochemical staining of human CD147 in human esophagus with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of squamous epithelium cell. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)
IHC (Immunohistochemistry) (Immunochemical staining of human CD147 in human gastric cancer with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.)
IHC (Immunohistchemistry) (Immunochemical staining of human CD147 in human liver with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of hepatocyte.)
IHC (Immunohistochemistry) (Immunochemical staining of human CD147 in human placenta with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing positive staining of trophoblast.)
IHC (Immunohistochemistry) (Immunochemical staining of human CD147 in human rectal cancer with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell in intestinal gland.)
IHC (Immunohistochemistry) (Immunochemical staining of human CD147 in human stomach with rabbit monoclonal antibody (1:5000, formalin-fixed paraffin embedded sections). The image showing membrane staining of epithelium cell.)
IF (Immunofluorescence) (Immunofluorescence staining of CD147 in monkey stomach with rabbit monoclonal antibody (1:1000, frozen section). The image showing membrane staining of gastric gland cell. The right panel: merge with DAPI)
IF (Immunofluorescence) (Immunofluorescence staining of human CD147 in HeLa cells with rabbit monoclonal antibody (1:200). The image showing membrane staining of HeLa cells.)
FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with CBL antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red).)
ICC (Immunocytochemistry) (ICC staining CBL (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded bladder cancer tissue using anti-CBL antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded ovarian cancer tissue using anti-CBL antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CBL on different cell lysates using anti-CBL antibody at 1/1, 000 dilution. Positive control: Lane 1: RAJI Lane 2: RAW264.7 Lane 3: K562 Lane 4: SKBR-3 Lane 5: 3T3-L1 Lane 6: THP-1 Lane 7: PC-12)
WB (Western Blot) (Western blot analysis of CBL on human CBL recombinant protein using anti-CBL antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-MARS mAb. [Lot No. MARSD10B4-2])
FCM (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-MARS mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. MARSD10B4-3])
IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-MARS mAb. [Lot No. MARSD10B4-2]Predicted molecular weight: 101 kDa)
WB (Western Blot) (Detection of MARS by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. MARSD10B4-3]Predicted molecular weight: 101 kDa)
Quality Control (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-MARS monoclonal antibody.)
SDS-PAGE (Whole cell lysates of MDCK were separated by 12% SDS-PAGE, and the membrane was blotted with anti-C-Myc(PTR2340) antibody. The HRP-conjugated Goat anti-M)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-STAT3 mAb. [Lot No. STAAD22A-1])
FCM (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-STAT3 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. STAAD22A-2])
IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-STAT3 mAb. [Lot No. STAAD22A-2]Predicted molecular weight: 87 kDa)
WB (Western Blot) (Detection of STAT3 by Western blot.Samples: Whole cell lysate from human HeLa (H, 25 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. STAAD22A-2]Predicted molecular weight: 87 kDa)
Quality Control (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-STAT3 monoclonal antibody.)
WB (Western Blot) (Western blot analysis of BIRC5 over-expressed 293 cell line, cotransfected with BIRC5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BIRC5 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged BIRC5 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of BIRC5 transfected lysate using BIRC5 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with BIRC5 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BIRC5 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of BIRC5 expression in transfected 293T cell line by BIRC5 monoclonal antibody. Lane 1: BIRC5 transfected lysate (16.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using Ring1 mouse mAb with DAB staining.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using Ring1 mouse mAb with DAB staining.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells using Ring1 mouse mAb (green) and negative control (red).)
WB (Western Blot) (Western blot analysis using Ring1 mouse mAb against MOLT-4 (1), LNCaP (2), Hela (3), HEK-293 (4) and Jurkat (5) cell lysate.)
ICC (Immunocytochemistry) (Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using anti-Ring1A mouse mAb (dilution 1:200).)
WB (Western Blot) (Western blot analysis of extracts from Jurkat, A549, MCF7 and C6 cell lysates using Ring1A mouse mAb (1:3000 diluted).Predicted band size:42KDa.Observed band size:54KDa.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BTAF1 mAb. [Lot No. BTA3D61-1])
FCM (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-BTAF1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. BTA3D61-1])
IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-BTAF1 mAb. [Lot No. BTA3D61-1]Predicted molecular weight: 206 kDa)
WB (Western Blot) (Detection of BTAF1 by Western blot.Samples: Whole cell lysate from human HeLa (H, 50 ug), mouse NIH3T3 (M, 50 ug) and rat F2408 (R, 50 ug) cells. [Lot No. BTA3D61-1]Predicted molecular weight: 206 kDa)
Quality Control (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control (Western blot analysis of immunized recombinant protein, using anti-BTAF1 monoclonal antibody.)
Application Data (Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (HDAC1 monoclonal antibody (M06), clone 1D6 Western Blot analysis of HDAC1 expression in Hela S3 NE.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged TP53 is approximately 3ng/ml as a capture antibody.)
WB (Western Blot) (TP53 monoclonal antibody, Western Blot analysis of TP53 expression in A-431.)
WB (Western Blot) (Western Blot detection against Immunogen (37.62kD.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TP53 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 6ug/ml])
Application Data (Proximity Ligation Analysis of protein-protein interactions between TP53 and BAX. HeLa cells were stained with TP53 rabbit purified polyclonal 1:1200 and BAX mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
WB (Western Blot) (Western Blot analysis of TP53 expression in transfected 293T cell line by TP53 monoclonal antibody. Lane 1: TP53 transfected lysate (43.34kD) Lane 2: Non-transfected lysate.)
ICC (Immunocytochemistry) (ICC staining Phospho-c-Jun (S63) in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-c-Jun (S63) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-c-Jun (S63) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-c-Jun (S63) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-c-Jun (S63) antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Phospho-c-Jun (S63) on different lysates using anti-Phospho-c-Jun (S63) antibody at 1/1, 000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T)
FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with 14-3-3 epsilon antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining 14-3-3 epsilon in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining 14-3-3 epsilon in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining 14-3-3 epsilon in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-14-3-3 epsilon antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-14-3-3 epsilon antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of 14-3-3 epsilon on different lysates using anti-14-3-3 epsilon antibody at 1/1, 000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: 293T)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Biotin)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:RPE)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Azide Free)
Application Data (Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 followed by the Histar detection system . Low power)
Application Data (Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 followed by the Histar detection system . High power)
Application Data (Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 followed by the Histar detection system . Low power)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Alexa Fluor 647)
Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 , red in A and Mousse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 , red in A and Mousse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. medium power)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:FITC)
WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in NIH/3T3.)
WB (Western Blot) (Western Blot analysis of DLX5 expression in transfected 293T cell line by DLX5 monoclonal antibody (M12). Lane 1: DLX5 transfected lysate (31.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (DLX5 monoclonal antibody, Western Blot analysis of DLX5 expression in A-431.)
WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in Raw 264.7.)
WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (35.79kD).)
WB (Western Blot) (CMAS monoclonal antibody. Western Blot analysis of CMAS expression in Raw 264.7.)
WB (Western Blot) (CMAS monoclonal antibody, Western Blot analysis of CMAS expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged CMAS is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CMAS on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CMAS on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3ug/ml].)
WB (Western Blot) (CMAS monoclonal antibody. Western Blot analysis of CMAS expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Cullin 4a antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Cullin 4a in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cullin 4a in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human cervix tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Cullin 4a antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Cullin 4a on Hela cell lysates using anti-Cullin 4a antibody at 1/500 dilution.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
Application Data (Detection limit for recombinant GST tagged GMNN is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GMNN on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of GMNN expression in transfected 293T cell line by GMNN monoclonal antibody. Lane 1: GMNN transfected lysate (23.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (FGR monoclonal antibody (M01), clone 3G10. Western Blot analysis of FGR expression in Raw 264.7 (Cat # L024V1).)
WB (Western Blot) (FGR monoclonal antibody (M01), clone 3G10. Western Blot analysis of FGR expression in PC-12 (Cat # L012V1).)
Application Data (Detection limit for recombinant GST tagged FGR is approximately 0.3ng/ml as a capture antibody.)
WB (Western Blot) (FGR monoclonal antibody (M01), clone 3G10 Western Blot analysis of FGR expression in HeLa (Cat # L013V1).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml])
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in Raw 264.7.)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged ZNF622 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ZNF622 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of ZNF622 expression in transfected 293T cell line by ZNF622 monoclonal antibody. Lane 1: ZNF622 transfected lysate (54.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (Detection of human c-Maf by western blot of HEK293T transfected with myc tagged human or mouse MafA, MafB, or c-Maf. Antibody: Rabbit anti-c-Maf recombinant monoclonal antibody (AAA23812 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Myc Tag recombinant monoclonal antibody .)
WB (Western Blot) (Detection of human c-Maf by western blot. Samples: Whole cell lysate (50 ug) from RPMI-8226, Hep-G2, 786-O, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-c-Maf recombinant monoclonal antibody [BL-664A-5F2] (AAA23812 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation) (Detection of human c-Maf by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-c-Maf recombinant monoclonal antibody [BL-664A-5F2] (AAA23812 lot 3) used for IP at 6 ul per reaction. c-Maf was also immunoprecipitated by a previous lot of this antibody (lot 2). For blotting immunoprecipitated c-Maf, AAA23812 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry) (Detection of mouse c-MAF by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-c-MAF recombinant monoclonal antibody (AAA23812 lot 3) used at 1:125. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human c-MAF by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-c-MAF recombinant monoclonal antibody (AAA23812 lot 3) used at 1:125. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human c-MAF by immunocytochemistry. Sample: FFPE section of RPMI-8226 cells. Antibody: Rabbit anti-c-MAF recombinant monoclonal antibody (AAA23812 lot 3) used at 1:125. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)
Application Data (Detection limit for recombinant GST tagged MCM2 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of MCM2 transfected lysate using MCM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MCM2 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM2 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody. Lane 1: MCM2 transfected lysate (101.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (MCM2 monoclonal antibody Western Blot analysis of MCM2 expression in HeLa NE.)
IF (Immunofluorescence) (Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence) (Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence) (Confocal immunofluorescent analysis of CD138 Antibody (C-term) (Ascites) with U266 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
FCM (Flow Cytometry) (CD138 Antibody (C-term) (Ascites) flow cytometric analysis of T47D cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)
FCM (Flow Cytometry) (CD138 Antibody (C-term) (Ascites) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)
FCM (Flow Cytometry) (CD138 Antibody (C-term) (Ascites) flow cytometric analysis of U266 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-mouse lgG secondary antibodies were used for the analysis)
WB (Western Blot) (CD138 Antibody (C-term)(Ascites) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the CD138 antibody detected the CD138 protein (arrow).)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (FGL2 monoclonal antibody Western Blot analysis of FGL2 expression in PC-12.)
Application Data (Detection limit for recombinant GST tagged FGL2 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of FGL2 transfected lysate using FGL2 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with FGL2 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FGL2 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 0.5ug/ml].)
WB (Western Blot) (Western Blot analysis of FGL2 expression in transfected 293T cell line by FGL2 monoclonal antibody Lane 1: FGL2 transfected lysate (50kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (FGL2 monoclonal antibody Western Blot analysis of FGL2 expression in Raw 264.7.)
FCM (Flow Cytometry) (Flow cytometric analysis of K562 cells with CRM1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining CRM1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CRM1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CRM1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-CRM1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-CRM1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CRM1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CRM1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CRM1 on 293T and Hela cell lysates using anti-CRM1 antibody at 1/500 dilution.)
Application Data (Proximity Ligation Analysis of protein-protein interactions between STAT1 and PDGFRB Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal (1:1200) and 131071 (1:50). Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged PDGFRB is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PDGFRB transfected lysate using 131071 and Protein A Magnetic Bead, and immunoblotted with PDGFRB rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human uterus myoma using 131071.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human stomach using 131071.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 0.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 0.5 ug/ml])
Application Data (Detection limit for recombinant GST tagged FOXA2 is approximately 0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (FOXA2 monoclonal antibody (M12), clone 6C12 Western Blot analysis of FOXA2 expression in K-562 (Cat # L009V1).)
Application Data (Detection limit for recombinant GST tagged CSE1L is approximately 3ng/ml as a capture antibody.)
WB (Western Blot) (CSE1L monoclonal antibody (M08), clone 3D8 Western Blot analysis of CSE1L expression in Hela S3 NE.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged PTBP1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PTBP1 transfected lysate using PTBP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PTBP1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PTBP1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PTBP1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of PTBP1 expression in transfected 293T cell line by PTBP1 monoclonal antibody, Lane 1: PTBP1 transfected lysate (59.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PTBP1 monoclonal antibody, Western Blot analysis of PTBP1 expression in A-431.)
PTBP1 (Polypyrimidine Tract-binding Protein 1, PTB, Heterogeneous Nuclear Ribonucleoprotein I, hnRNP I, 57kD RNA-binding Protein PPTB-1, MGC10830, MGC8461) (PE)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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