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Product Notes
The Cas9 (Catalog #AAA11444) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Cas9 (CRISPR Associated Protein 9), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What does a Cas9 ELISA kit measure in CRISPR experiments?
A Cas9 ELISA kit measures how much Cas9 protein is present in a sample. In CRISPR experiments, it helps researchers check whether the Cas9 protein is there and in what amount, so they know if the CRISPR system is being used properly.
Can a Cas9 ELISA kit quantify Cas9 protein expression in cells?
Yes, the kit can measure how much Cas9 protein is produced inside cells. Scientists use it to confirm that cells are successfully making Cas9 after CRISPR delivery, which helps them understand how well the experiment is working.
Is this kit suitable for detecting Cas9 in cell lysates or culture media?
Yes, this kit can detect Cas9 protein in cell lysates and sometimes in culture media. This allows researchers to test samples taken from broken cells or liquids where cells grow, making it useful for different lab experiments.
How is Cas9 ELISA used to monitor CRISPR delivery efficiency?
Cas9 ELISA is used to see if Cas9 was successfully delivered into cells. By measuring how much Cas9 protein is present, researchers can tell whether the CRISPR tools entered the cells properly and how effective their delivery method was.
Can this assay distinguish active Cas9 from degraded protein?
No, this ELISA test mainly measures the total amount of Cas9 protein. It can't confirm if Cas9 is active or degraded. Other tests are needed to check if the protein still works.
Is Cas9 ELISA commonly used in gene-editing validation studies?
Yes, Cas9 ELISA is commonly used in gene-editing studies. It confirms Cas9 protein presence in experiments, a key step to verify correct CRISPR gene editing.
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