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WB (Western Blot)
(Sample: Recombinant MMP9, Porcine; Antibody: Rabbit Anti-Porcine MMP9 Ab)
Matrix Metalloproteinase 9 (MMP9) Active Protein | MMP9 active protein
Active Matrix Metalloproteinase 9 (MMP9)
Gene Names
MMP9; GELB; CLG4B; MMP-9; MANDP2
Reactivity
Sus scrofa; Porcine (Pig)
Applications
Activity Assay, Cell Culture
Purity
>94%
Synonyms
Matrix Metalloproteinase 9 (MMP9); N/A; Active Matrix Metalloproteinase 9 (MMP9); GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase; MMP9 active protein
Host
E. coli
Reactivity
Sus scrofa; Porcine (Pig)
Purity/Purification
>94%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl and 5% trehalose.
Applicable Applications for MMP9 active protein
Activity Assay, Cell Culture
Source
Prokaryotic expression.
Residues
Ile169~Glu387
Tags
N-terminal His-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted isoelectric point
4.8
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Sample: Recombinant MMP9, Porcine; Antibody: Rabbit Anti-Porcine MMP9 Ab)
WB (Western Blot)
(Sample: Recombinant MMP9, Porcine; Antibody: Rabbit Anti-Porcine MMP9 Ab)
SDS_PAGE
(Sample: Active recombinant MMP9, Porcine)
SDS_PAGE
(Sample: Active recombinant MMP9, Porcine)
Gene Sequencing
(Gene Sequencing (extract))
Gene Sequencing
(Gene Sequencing (extract))
Bioactivity
(Mechanism: MMP9 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 92kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of recombinant pig MMP9 (1000ng, 500ng, 100ng, 10ng, 1ng, 0.1ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphatepolyacrylamide gel (SDS–PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP9 protein as negative control, and blood sample as positive control. Result: Gelatin hydrolysis by recombinant pig MMP9 (10-70kd) was shown in figure 1.)
Bioactivity
(Mechanism: MMP9 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 92kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9 and It is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of recombinant pig MMP9 (1000ng, 500ng, 100ng, 10ng, 1ng, 0.1ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphatepolyacrylamide gel (SDS–PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP9 protein as negative control, and blood sample as positive control. Result: Gelatin hydrolysis by recombinant pig MMP9 (10-70kd) was shown in figure 1.)
Sequence
Sequence
Product Categories/Family for MMP9 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 27.9kDa
Accurate Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 30kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
Matrix metalloproteinase-9
NCBI Official Synonym Full Names
matrix metallopeptidase 9
NCBI Official Symbol
MMP9
NCBI Official Synonym Symbols
GELB; CLG4B; MMP-9; MANDP2
NCBI Protein Information
matrix metalloproteinase-9
UniProt Protein Name
Matrix metalloproteinase-9
UniProt Gene Name
MMP9
UniProt Synonym Gene Names
CLG4B; MMP-9; GELB
UniProt Entry Name
MMP9_HUMAN
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Product Notes
The MMP9 mmp9 (Catalog #AAA149237) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Matrix Metalloproteinase 9 (MMP9) reacts with Sus scrofa; Porcine (Pig) and may cross-react with other species as described in the data sheet. AAA Biotech's Matrix Metalloproteinase 9 (MMP9) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the MMP9 mmp9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Matrix Metalloproteinase 9 (MMP9), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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