SDS-PAGE
Diazepam Binding Inhibitor (DBI) Recombinant Protein | DBI recombinant protein
Recombinant Diazepam Binding Inhibitor (DBI)
Gene Names
Dbi; EP; Acbp; ACBD1; endozepine
Applications
Western Blot, SDS-Page
Purity
> 95%
Synonyms
Diazepam Binding Inhibitor (DBI); N/A; Recombinant Diazepam Binding Inhibitor (DBI); DBI recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Concentration
Original Concentration: 200 Ug/mL (varies by lot)
Sequence
SQAEFDKAA EEVKRLKTQP TDEEMMLFIYS HFKQATVGDV NTDRPGLLDL KGKAKWDSWN KLKGTSKESA MKTYVEKVDE LKKKYGI
Applicable Applications for DBI recombinant protein
WB (Western Blot), SDS-PAGE, Immunogen, Positive Control
Organism Species
Mus musculus
Source
Prokaryotic expression
Residues
Ser2~Ile87
Tags
N-terminal His Tag
Traits
Freeze-dried powder
Buffer
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose and Proclin300.
USAGE
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Subcellular Location
Endoplasmic reticulum lumen
Predicted isoelectric point
8.0
Predicted Molecular Mass
11.1kDa
Accurate Molecular Mass
13kDa as determined by SDS-PAGE reducing conditions.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-PAGE
SDS-PAGE
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
acyl-CoA-binding protein isoform 2
NCBI Official Synonym Full Names
diazepam binding inhibitor
NCBI Official Symbol
Dbi
NCBI Official Synonym Symbols
EP; Acbp; ACBD1; endozepine
NCBI Protein Information
acyl-CoA-binding protein; acyl-CoA binding protein; diazepam-binding inhibitor; diazepam binding inhibitor, splice form 1b
UniProt Protein Name
Acyl-CoA-binding protein
UniProt Gene Name
Dbi
UniProt Synonym Gene Names
ACBP; DBI; EP
UniProt Entry Name
ACBP_MOUSE
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Product Notes
The DBI dbi (Catalog #AAA134310) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Diazepam Binding Inhibitor (DBI) can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), SDS-PAGE, Immunogen, Positive Control. Researchers should empirically determine the suitability of the DBI dbi for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SQAEFDKAA EEVKRLKTQP TDEEMMLFIY S HFKQATVGDV NTDRPGLLDL KGKAKWDSWN KLKGTSKESA MKTYVEKVDE LKKKYGI. It is sometimes possible for the material contained within the vial of "Diazepam Binding Inhibitor (DBI), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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