Application Data
Prestained Multicolor Protein Molecular Weight Marker (1.7-40 kDa) Accessory Reagent
Prestained Multicolor Protein Molecular Weight Marker (1.7-40 kDa)
Verifying Western transfer efficiency.
Approximate sizing of proteins on SDS-PAGE and Western blots.
2. Mix gently, but thoroughly, to ensure that the solution is homogeneous.
3. Load the following volumes of the ladder on
Min SDS-polyacrylamide gel: 5 ul per well, with a thickness of 0.75-1.0 mm
Large SDS-polyacrylamide gel: 10 ul per well, with a thickness of 0.75-1.0 mm.
Application Data
Application Data
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Product Notes
The Prestained Multicolor Protein Molecular Weight Marker (1.7-40 kDa) (Catalog #AAA13522) is an Accessory Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Prestained Multicolor Protein Molecular Weight Marker (1.7-40 kDa) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, WB (Western Blot). Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis. Verifying Western transfer efficiency. Approximate sizing of proteins on SDS-PAGE and Western blots. Researchers should empirically determine the suitability of the Prestained Multicolor Protein Molecular Weight Marker (1.7-40 kDa) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prestained Multicolor Protein Molecular Weight Marker (1.7-40 kDa), Accessory Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Frequently Asked Questions
What is a prestained multicolor protein molecular weight marker used for?
A prestained multicolor protein marker is used to estimate the molecular weight of proteins during SDS-PAGE and to monitor the progress of the electrophoresis run in real-time. It also serves as a visual indicator of transfer efficiency from the gel to the membrane during Western blotting, a process frequently performed using AAA Biotech laboratory reagents.
How does a prestained protein ladder help estimate protein size?
The ladder consists of several purified proteins of known molecular weights, each covalently bound to a colored dye. By comparing the position of your target protein band to the colored bands of the AAA Biotech ladder, you can estimate the size of your protein in kilodaltons (kDa). The multicolor format makes it easier to identify specific reference peaks quickly.
What is the advantage of using a multicolor protein marker?
The primary advantage is the ease of band identification. By using different colors (such as green, red, or orange) for specific reference bands, the AAA Biotech multicolor marker prevents confusion during electrophoresis and membrane orientation. This is particularly helpful when working with a wide range of proteins, as it provides clear "anchor points" for size estimation.
Can prestained protein markers be used for SDS-PAGE and Western blot?
Yes, they are designed for both applications. During SDS-PAGE, the colors allow you to see the separation as it happens. During Western blotting, the prestained proteins transfer onto the membrane along with your samples. This allows AAA Biotech users to confirm that the transfer was successful and provides a permanent size reference directly on the blot for downstream detection.
Do prestained markers affect protein migration accuracy?
Prestained markers provide an excellent estimate of size, though the attached dye can slightly shift the migration compared to unstained proteins. For most analytical purposes, this minor difference is negligible. AAA Biotech’s multicolor markers are calibrated against unstained standards to ensure they provide a reliable and consistent scale for daily research and diagnostic applications.
What molecular weight range does a prestained protein marker cover?
AAA Biotech offers markers that typically cover a broad range, such as 10 kDa to 250 kDa. This range is suitable for detecting the majority of proteins studied in biological research. The combination of low, medium, and high molecular weight bands in a single ladder ensures that researchers can accurately characterize both small peptides and large multi-subunit complexes.
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